Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_33 and Pf1N1B4_34 are separated by 152 nucleotides Pf1N1B4_34 and Pf1N1B4_35 are separated by 432 nucleotides Pf1N1B4_35 and Pf1N1B4_36 are separated by 3 nucleotides
Pf1N1B4_33 - Outer membrane component of tripartite multidrug resistance system, at 37,992 to 39,422
_33
Pf1N1B4_34 - Xanthine permease, at 39,575 to 41,029
_34
Pf1N1B4_35 - hypothetical protein, at 41,462 to 41,629
_35
Pf1N1B4_36 - hypothetical protein, at 41,633 to 41,977
_36
Position (kb) 39
40
41
42 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 38.703 kb on + strand at 38.703 kb on + strand at 38.703 kb on + strand, within Pf1N1B4_33 at 38.703 kb on + strand, within Pf1N1B4_33 at 38.704 kb on - strand at 38.704 kb on - strand at 38.704 kb on - strand, within Pf1N1B4_33 at 38.706 kb on - strand, within Pf1N1B4_33 at 38.721 kb on - strand, within Pf1N1B4_33 at 38.863 kb on - strand, within Pf1N1B4_33 at 38.904 kb on - strand, within Pf1N1B4_33 at 38.968 kb on + strand, within Pf1N1B4_33 at 38.968 kb on + strand, within Pf1N1B4_33 at 38.969 kb on - strand at 39.156 kb on + strand, within Pf1N1B4_33 at 39.189 kb on + strand, within Pf1N1B4_33 at 39.548 kb on + strand at 39.691 kb on - strand at 39.883 kb on - strand, within Pf1N1B4_34 at 40.501 kb on + strand, within Pf1N1B4_34 at 40.501 kb on + strand, within Pf1N1B4_34 at 40.501 kb on + strand, within Pf1N1B4_34 at 40.995 kb on + strand at 40.995 kb on + strand at 40.996 kb on - strand at 40.996 kb on - strand at 40.996 kb on - strand at 41.030 kb on - strand at 41.133 kb on - strand at 41.167 kb on - strand at 41.169 kb on - strand at 41.249 kb on + strand at 41.250 kb on - strand at 41.250 kb on - strand at 41.250 kb on - strand at 41.330 kb on - strand at 41.336 kb on - strand at 41.336 kb on - strand at 41.543 kb on + strand, within Pf1N1B4_35 at 41.544 kb on - strand at 41.544 kb on - strand, within Pf1N1B4_35 at 41.544 kb on - strand, within Pf1N1B4_35 at 41.544 kb on - strand, within Pf1N1B4_35 at 41.544 kb on - strand, within Pf1N1B4_35 at 41.544 kb on - strand, within Pf1N1B4_35 at 41.544 kb on - strand, within Pf1N1B4_35 at 41.544 kb on - strand, within Pf1N1B4_35 at 41.549 kb on + strand at 41.615 kb on + strand at 41.615 kb on + strand at 41.616 kb on - strand at 41.616 kb on - strand at 41.616 kb on - strand at 41.616 kb on - strand at 41.616 kb on - strand at 41.616 kb on - strand at 41.616 kb on - strand at 41.618 kb on - strand at 41.618 kb on - strand at 41.646 kb on - strand at 41.731 kb on - strand, within Pf1N1B4_36 at 41.791 kb on + strand, within Pf1N1B4_36 at 41.792 kb on - strand, within Pf1N1B4_36 at 41.975 kb on + strand at 41.976 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 38,703 + +1.7 38,703 + +0.7 38,703 + Pf1N1B4_33 0.50 -1.3 38,703 + Pf1N1B4_33 0.50 +0.3 38,704 - +1.7 38,704 - -1.9 38,704 - Pf1N1B4_33 0.50 -0.5 38,706 - Pf1N1B4_33 0.50 +0.7 38,721 - Pf1N1B4_33 0.51 -0.3 38,863 - Pf1N1B4_33 0.61 +0.7 38,904 - Pf1N1B4_33 0.64 +0.1 38,968 + Pf1N1B4_33 0.68 -0.3 38,968 + Pf1N1B4_33 0.68 -1.9 38,969 - -1.3 39,156 + Pf1N1B4_33 0.81 -0.9 39,189 + Pf1N1B4_33 0.84 -0.3 39,548 + -0.6 39,691 - -0.7 39,883 - Pf1N1B4_34 0.21 -2.0 40,501 + Pf1N1B4_34 0.64 +0.3 40,501 + Pf1N1B4_34 0.64 -1.3 40,501 + Pf1N1B4_34 0.64 -3.1 40,995 + +0.0 40,995 + -1.6 40,996 - -1.3 40,996 - -1.0 40,996 - -0.6 41,030 - -1.3 41,133 - +0.0 41,167 - +1.7 41,169 - -0.3 41,249 + -0.3 41,250 - -1.7 41,250 - +0.6 41,250 - +0.7 41,330 - +0.1 41,336 - -1.3 41,336 - -0.3 41,543 + Pf1N1B4_35 0.48 -0.3 41,544 - +0.7 41,544 - Pf1N1B4_35 0.49 -0.6 41,544 - Pf1N1B4_35 0.49 +1.7 41,544 - Pf1N1B4_35 0.49 -2.0 41,544 - Pf1N1B4_35 0.49 -2.3 41,544 - Pf1N1B4_35 0.49 -0.9 41,544 - Pf1N1B4_35 0.49 -0.3 41,544 - Pf1N1B4_35 0.49 -0.7 41,549 + -0.9 41,615 + +0.3 41,615 + +0.7 41,616 - -0.1 41,616 - -0.3 41,616 - +0.1 41,616 - +2.5 41,616 - -1.3 41,616 - -1.3 41,616 - -0.8 41,618 - +0.4 41,618 - +1.7 41,646 - -0.3 41,731 - Pf1N1B4_36 0.28 +1.3 41,791 + Pf1N1B4_36 0.46 -0.1 41,792 - Pf1N1B4_36 0.46 +2.0 41,975 + +0.1 41,976 - -1.9
Or see this region's nucleotide sequence