Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3348 and Pf1N1B4_3349 are separated by 277 nucleotides Pf1N1B4_3349 and Pf1N1B4_3350 are separated by 136 nucleotides Pf1N1B4_3350 and Pf1N1B4_3351 are separated by 127 nucleotides Pf1N1B4_3351 and Pf1N1B4_3352 are separated by 92 nucleotides
Pf1N1B4_3348 - Nitroreductase family protein, at 3,735,881 to 3,736,474
_3348
Pf1N1B4_3349 - YcgN (Fragment), at 3,736,752 to 3,737,201
_3349
Pf1N1B4_3350 - FIG00954807: hypothetical protein, at 3,737,338 to 3,738,567
_3350
Pf1N1B4_3351 - FIG00954302: hypothetical protein, at 3,738,695 to 3,739,072
_3351
Pf1N1B4_3352 - tRNA (guanosine(18)-2'-O)-methyltransferase (EC 2.1.1.34), at 3,739,165 to 3,739,635
_3352
Position (kb)
3737
3738
3739 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3736.447 kb on - strand at 3736.465 kb on + strand at 3736.489 kb on - strand at 3736.564 kb on + strand at 3736.564 kb on + strand at 3736.633 kb on + strand at 3736.633 kb on + strand at 3736.633 kb on + strand at 3736.634 kb on - strand at 3736.634 kb on - strand at 3736.634 kb on - strand at 3736.634 kb on - strand at 3736.646 kb on + strand at 3736.646 kb on + strand at 3736.648 kb on + strand at 3736.648 kb on + strand at 3736.648 kb on + strand at 3736.649 kb on - strand at 3736.649 kb on - strand at 3736.649 kb on - strand at 3736.649 kb on - strand at 3736.649 kb on - strand at 3736.706 kb on + strand at 3736.709 kb on + strand at 3736.709 kb on + strand at 3736.709 kb on + strand at 3736.710 kb on - strand at 3736.710 kb on - strand at 3736.887 kb on + strand, within Pf1N1B4_3349 at 3736.887 kb on + strand, within Pf1N1B4_3349 at 3736.888 kb on - strand, within Pf1N1B4_3349 at 3736.888 kb on - strand, within Pf1N1B4_3349 at 3736.888 kb on - strand, within Pf1N1B4_3349 at 3736.888 kb on - strand, within Pf1N1B4_3349 at 3736.888 kb on - strand, within Pf1N1B4_3349 at 3736.890 kb on + strand at 3736.890 kb on + strand, within Pf1N1B4_3349 at 3736.890 kb on + strand at 3736.890 kb on + strand, within Pf1N1B4_3349 at 3736.891 kb on - strand, within Pf1N1B4_3349 at 3736.891 kb on - strand, within Pf1N1B4_3349 at 3736.891 kb on - strand, within Pf1N1B4_3349 at 3736.891 kb on - strand, within Pf1N1B4_3349 at 3736.891 kb on - strand, within Pf1N1B4_3349 at 3736.937 kb on + strand, within Pf1N1B4_3349 at 3737.117 kb on + strand, within Pf1N1B4_3349 at 3737.117 kb on + strand, within Pf1N1B4_3349 at 3737.117 kb on + strand, within Pf1N1B4_3349 at 3737.117 kb on + strand, within Pf1N1B4_3349 at 3737.117 kb on + strand, within Pf1N1B4_3349 at 3737.117 kb on + strand, within Pf1N1B4_3349 at 3737.117 kb on + strand, within Pf1N1B4_3349 at 3737.117 kb on + strand, within Pf1N1B4_3349 at 3737.117 kb on + strand at 3737.118 kb on - strand, within Pf1N1B4_3349 at 3737.135 kb on + strand, within Pf1N1B4_3349 at 3737.135 kb on + strand, within Pf1N1B4_3349 at 3737.135 kb on + strand, within Pf1N1B4_3349 at 3737.136 kb on - strand, within Pf1N1B4_3349 at 3737.136 kb on - strand, within Pf1N1B4_3349 at 3737.136 kb on - strand, within Pf1N1B4_3349 at 3737.136 kb on - strand, within Pf1N1B4_3349 at 3737.199 kb on + strand at 3737.247 kb on + strand at 3737.248 kb on - strand at 3737.291 kb on + strand at 3737.327 kb on + strand at 3737.327 kb on + strand at 3737.328 kb on - strand at 3737.328 kb on - strand at 3737.337 kb on + strand at 3737.425 kb on + strand at 3737.783 kb on + strand, within Pf1N1B4_3350 at 3738.113 kb on - strand, within Pf1N1B4_3350 at 3738.241 kb on + strand, within Pf1N1B4_3350 at 3738.244 kb on + strand, within Pf1N1B4_3350 at 3738.245 kb on - strand, within Pf1N1B4_3350 at 3738.245 kb on - strand, within Pf1N1B4_3350 at 3738.565 kb on + strand at 3738.600 kb on + strand at 3738.601 kb on - strand at 3738.601 kb on - strand at 3738.601 kb on - strand at 3738.669 kb on + strand at 3738.911 kb on + strand, within Pf1N1B4_3351 at 3738.912 kb on - strand, within Pf1N1B4_3351 at 3738.912 kb on - strand, within Pf1N1B4_3351 at 3738.956 kb on + strand, within Pf1N1B4_3351 at 3738.956 kb on + strand, within Pf1N1B4_3351 at 3738.956 kb on + strand, within Pf1N1B4_3351 at 3738.956 kb on + strand, within Pf1N1B4_3351 at 3739.045 kb on + strand at 3739.046 kb on - strand at 3739.052 kb on + strand at 3739.052 kb on + strand at 3739.109 kb on + strand at 3739.109 kb on + strand at 3739.109 kb on + strand at 3739.109 kb on + strand at 3739.110 kb on - strand at 3739.110 kb on - strand at 3739.110 kb on - strand at 3739.110 kb on - strand at 3739.113 kb on + strand at 3739.113 kb on + strand at 3739.113 kb on + strand at 3739.114 kb on - strand at 3739.114 kb on - strand at 3739.114 kb on - strand at 3739.114 kb on - strand at 3739.117 kb on + strand at 3739.118 kb on - strand at 3739.118 kb on - strand at 3739.271 kb on + strand, within Pf1N1B4_3352 at 3739.271 kb on + strand, within Pf1N1B4_3352 at 3739.271 kb on + strand, within Pf1N1B4_3352 at 3739.272 kb on - strand, within Pf1N1B4_3352 at 3739.337 kb on + strand, within Pf1N1B4_3352 at 3739.337 kb on + strand, within Pf1N1B4_3352 at 3739.337 kb on + strand, within Pf1N1B4_3352 at 3739.337 kb on + strand at 3739.337 kb on + strand, within Pf1N1B4_3352 at 3739.338 kb on - strand, within Pf1N1B4_3352 at 3739.338 kb on - strand, within Pf1N1B4_3352 at 3739.338 kb on - strand, within Pf1N1B4_3352 at 3739.361 kb on + strand, within Pf1N1B4_3352 at 3739.361 kb on + strand, within Pf1N1B4_3352 at 3739.467 kb on - strand, within Pf1N1B4_3352
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 3,736,447 - +0.7 3,736,465 + +1.5 3,736,489 - -0.1 3,736,564 + -0.7 3,736,564 + -0.1 3,736,633 + -2.4 3,736,633 + -0.4 3,736,633 + +1.2 3,736,634 - -2.1 3,736,634 - -1.1 3,736,634 - +0.5 3,736,634 - +0.1 3,736,646 + -0.7 3,736,646 + -0.1 3,736,648 + +0.2 3,736,648 + -0.1 3,736,648 + -1.4 3,736,649 - +1.2 3,736,649 - -1.2 3,736,649 - -3.0 3,736,649 - -1.1 3,736,649 - +0.9 3,736,706 + -0.8 3,736,709 + -0.3 3,736,709 + -1.7 3,736,709 + -1.1 3,736,710 - -0.1 3,736,710 - +0.9 3,736,887 + Pf1N1B4_3349 0.30 -0.5 3,736,887 + Pf1N1B4_3349 0.30 -1.1 3,736,888 - Pf1N1B4_3349 0.30 -0.5 3,736,888 - Pf1N1B4_3349 0.30 +0.5 3,736,888 - Pf1N1B4_3349 0.30 +0.2 3,736,888 - Pf1N1B4_3349 0.30 +0.7 3,736,888 - Pf1N1B4_3349 0.30 -0.1 3,736,890 + -0.5 3,736,890 + Pf1N1B4_3349 0.31 -2.1 3,736,890 + +1.2 3,736,890 + Pf1N1B4_3349 0.31 -0.1 3,736,891 - Pf1N1B4_3349 0.31 -0.4 3,736,891 - Pf1N1B4_3349 0.31 -0.5 3,736,891 - Pf1N1B4_3349 0.31 +0.1 3,736,891 - Pf1N1B4_3349 0.31 -0.1 3,736,891 - Pf1N1B4_3349 0.31 -0.1 3,736,937 + Pf1N1B4_3349 0.41 +0.7 3,737,117 + Pf1N1B4_3349 0.81 -0.1 3,737,117 + Pf1N1B4_3349 0.81 +0.7 3,737,117 + Pf1N1B4_3349 0.81 +0.1 3,737,117 + Pf1N1B4_3349 0.81 -0.7 3,737,117 + Pf1N1B4_3349 0.81 +0.9 3,737,117 + Pf1N1B4_3349 0.81 -0.5 3,737,117 + Pf1N1B4_3349 0.81 -0.3 3,737,117 + Pf1N1B4_3349 0.81 -0.7 3,737,117 + -0.1 3,737,118 - Pf1N1B4_3349 0.81 -2.9 3,737,135 + Pf1N1B4_3349 0.85 +0.9 3,737,135 + Pf1N1B4_3349 0.85 -2.9 3,737,135 + Pf1N1B4_3349 0.85 -0.4 3,737,136 - Pf1N1B4_3349 0.85 +1.8 3,737,136 - Pf1N1B4_3349 0.85 +0.5 3,737,136 - Pf1N1B4_3349 0.85 -0.8 3,737,136 - Pf1N1B4_3349 0.85 -0.7 3,737,199 + +0.7 3,737,247 + -0.8 3,737,248 - +0.3 3,737,291 + -0.1 3,737,327 + +2.2 3,737,327 + +0.2 3,737,328 - -0.1 3,737,328 - +0.9 3,737,337 + -2.4 3,737,425 + +0.9 3,737,783 + Pf1N1B4_3350 0.36 -0.1 3,738,113 - Pf1N1B4_3350 0.63 +1.5 3,738,241 + Pf1N1B4_3350 0.73 +0.7 3,738,244 + Pf1N1B4_3350 0.74 +0.7 3,738,245 - Pf1N1B4_3350 0.74 -1.1 3,738,245 - Pf1N1B4_3350 0.74 -0.1 3,738,565 + -1.3 3,738,600 + -0.5 3,738,601 - -0.1 3,738,601 - -0.1 3,738,601 - +1.7 3,738,669 + -0.7 3,738,911 + Pf1N1B4_3351 0.57 -0.4 3,738,912 - Pf1N1B4_3351 0.57 -0.7 3,738,912 - Pf1N1B4_3351 0.57 -1.4 3,738,956 + Pf1N1B4_3351 0.69 +0.9 3,738,956 + Pf1N1B4_3351 0.69 -0.8 3,738,956 + Pf1N1B4_3351 0.69 -1.1 3,738,956 + Pf1N1B4_3351 0.69 +1.5 3,739,045 + -0.1 3,739,046 - -0.7 3,739,052 + -2.9 3,739,052 + +1.5 3,739,109 + +0.5 3,739,109 + -0.7 3,739,109 + -1.1 3,739,109 + -0.2 3,739,110 - -0.5 3,739,110 - +0.9 3,739,110 - +0.3 3,739,110 - -1.4 3,739,113 + +0.5 3,739,113 + -2.4 3,739,113 + -0.3 3,739,114 - +0.7 3,739,114 - +0.3 3,739,114 - +1.5 3,739,114 - -1.7 3,739,117 + -1.7 3,739,118 - +1.2 3,739,118 - +0.7 3,739,271 + Pf1N1B4_3352 0.23 +1.2 3,739,271 + Pf1N1B4_3352 0.23 +0.3 3,739,271 + Pf1N1B4_3352 0.23 -0.1 3,739,272 - Pf1N1B4_3352 0.23 +2.2 3,739,337 + Pf1N1B4_3352 0.37 +1.5 3,739,337 + Pf1N1B4_3352 0.37 +0.2 3,739,337 + Pf1N1B4_3352 0.37 +0.7 3,739,337 + +0.9 3,739,337 + Pf1N1B4_3352 0.37 -0.7 3,739,338 - Pf1N1B4_3352 0.37 -1.7 3,739,338 - Pf1N1B4_3352 0.37 -1.3 3,739,338 - Pf1N1B4_3352 0.37 +0.9 3,739,361 + Pf1N1B4_3352 0.42 -0.3 3,739,361 + Pf1N1B4_3352 0.42 -0.1 3,739,467 - Pf1N1B4_3352 0.64 -0.1
Or see this region's nucleotide sequence