Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_325 and Pf1N1B4_326 are separated by 443 nucleotides Pf1N1B4_326 and Pf1N1B4_327 are separated by 2 nucleotides Pf1N1B4_327 and Pf1N1B4_328 are separated by 905 nucleotides
Pf1N1B4_325 - D amino acid oxidase (DAO) family (EC 1.4.3.3), at 383,627 to 384,769
_325
Pf1N1B4_326 - Glycosyl transferase, family 2:GtrA-like protein, at 385,213 to 385,503
_326
Pf1N1B4_327 - hypothetical protein, at 385,506 to 386,450
_327
Pf1N1B4_328 - Glucosyltransferase (side chain biosynthesis) (EC 2.4.1.-), at 387,356 to 388,624
_328
Position (kb) 385
386
387 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 384.525 kb on + strand, within Pf1N1B4_325 at 384.525 kb on + strand, within Pf1N1B4_325 at 384.525 kb on + strand, within Pf1N1B4_325 at 384.525 kb on + strand, within Pf1N1B4_325 at 384.525 kb on + strand, within Pf1N1B4_325 at 384.525 kb on + strand, within Pf1N1B4_325 at 384.525 kb on + strand, within Pf1N1B4_325 at 384.525 kb on + strand, within Pf1N1B4_325 at 384.526 kb on - strand, within Pf1N1B4_325 at 384.526 kb on - strand, within Pf1N1B4_325 at 384.526 kb on - strand, within Pf1N1B4_325 at 384.533 kb on + strand, within Pf1N1B4_325 at 384.533 kb on + strand, within Pf1N1B4_325 at 384.560 kb on + strand, within Pf1N1B4_325 at 384.560 kb on + strand, within Pf1N1B4_325 at 384.560 kb on + strand, within Pf1N1B4_325 at 384.561 kb on - strand, within Pf1N1B4_325 at 384.561 kb on - strand, within Pf1N1B4_325 at 384.564 kb on + strand, within Pf1N1B4_325 at 384.565 kb on - strand at 384.565 kb on - strand, within Pf1N1B4_325 at 384.596 kb on - strand, within Pf1N1B4_325 at 384.663 kb on - strand at 384.695 kb on + strand at 384.696 kb on - strand at 384.696 kb on - strand at 384.696 kb on - strand at 384.847 kb on + strand at 384.850 kb on - strand at 385.061 kb on + strand at 385.062 kb on - strand at 385.196 kb on + strand at 385.587 kb on + strand at 385.590 kb on - strand at 385.787 kb on - strand, within Pf1N1B4_327 at 385.787 kb on - strand, within Pf1N1B4_327 at 385.904 kb on - strand, within Pf1N1B4_327 at 385.904 kb on - strand, within Pf1N1B4_327 at 386.938 kb on + strand at 386.939 kb on - strand at 386.940 kb on + strand at 387.303 kb on + strand at 387.304 kb on - strand at 387.305 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 384,525 + Pf1N1B4_325 0.79 -1.0 384,525 + Pf1N1B4_325 0.79 +0.4 384,525 + Pf1N1B4_325 0.79 -0.8 384,525 + Pf1N1B4_325 0.79 -0.8 384,525 + Pf1N1B4_325 0.79 -1.0 384,525 + Pf1N1B4_325 0.79 +0.4 384,525 + Pf1N1B4_325 0.79 -1.2 384,525 + Pf1N1B4_325 0.79 -0.2 384,526 - Pf1N1B4_325 0.79 -0.2 384,526 - Pf1N1B4_325 0.79 -1.2 384,526 - Pf1N1B4_325 0.79 -0.2 384,533 + Pf1N1B4_325 0.79 +1.0 384,533 + Pf1N1B4_325 0.79 -0.1 384,560 + Pf1N1B4_325 0.82 +1.8 384,560 + Pf1N1B4_325 0.82 -0.2 384,560 + Pf1N1B4_325 0.82 -0.2 384,561 - Pf1N1B4_325 0.82 -0.5 384,561 - Pf1N1B4_325 0.82 -1.2 384,564 + Pf1N1B4_325 0.82 -1.1 384,565 - -0.2 384,565 - Pf1N1B4_325 0.82 +0.4 384,596 - Pf1N1B4_325 0.85 -1.0 384,663 - -1.5 384,695 + +1.0 384,696 - -0.6 384,696 - -1.2 384,696 - +0.0 384,847 + +0.8 384,850 - -1.2 385,061 + -2.7 385,062 - -3.5 385,196 + +0.8 385,587 + -3.5 385,590 - -2.4 385,787 - Pf1N1B4_327 0.30 -0.2 385,787 - Pf1N1B4_327 0.30 -2.5 385,904 - Pf1N1B4_327 0.42 +1.1 385,904 - Pf1N1B4_327 0.42 -1.8 386,938 + -3.0 386,939 - +0.4 386,940 + -1.5 387,303 + -1.5 387,304 - -1.8 387,305 + +0.2
Or see this region's nucleotide sequence