Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3240 and Pf1N1B4_3241 are separated by 616 nucleotides Pf1N1B4_3241 and Pf1N1B4_3242 are separated by 192 nucleotides Pf1N1B4_3242 and Pf1N1B4_3243 are separated by 15 nucleotides
Pf1N1B4_3240 - PhaK-like protein, at 3,626,273 to 3,627,532
_3240
Pf1N1B4_3241 - hypothetical protein, at 3,628,149 to 3,628,412
_3241
Pf1N1B4_3242 - GCN5-related N-acetyltransferase, at 3,628,605 to 3,629,000
_3242
Pf1N1B4_3243 - hypothetical protein, at 3,629,016 to 3,630,176
_3243
Position (kb)
3628
3629 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3627.261 kb on + strand, within Pf1N1B4_3240 at 3627.261 kb on + strand, within Pf1N1B4_3240 at 3627.262 kb on - strand, within Pf1N1B4_3240 at 3627.262 kb on - strand at 3627.262 kb on - strand, within Pf1N1B4_3240 at 3627.386 kb on - strand, within Pf1N1B4_3240 at 3627.396 kb on + strand, within Pf1N1B4_3240 at 3627.396 kb on + strand, within Pf1N1B4_3240 at 3627.510 kb on + strand at 3627.510 kb on + strand at 3627.511 kb on - strand at 3627.511 kb on - strand at 3627.511 kb on - strand at 3627.549 kb on - strand at 3627.554 kb on + strand at 3627.554 kb on + strand at 3627.554 kb on + strand at 3627.554 kb on + strand at 3627.555 kb on - strand at 3627.570 kb on - strand at 3627.685 kb on + strand at 3627.685 kb on + strand at 3627.686 kb on - strand at 3627.767 kb on + strand at 3627.807 kb on + strand at 3627.812 kb on - strand at 3628.158 kb on - strand at 3628.228 kb on + strand, within Pf1N1B4_3241 at 3628.273 kb on + strand, within Pf1N1B4_3241 at 3628.273 kb on + strand, within Pf1N1B4_3241 at 3628.274 kb on - strand, within Pf1N1B4_3241 at 3628.277 kb on + strand, within Pf1N1B4_3241 at 3628.478 kb on + strand at 3628.478 kb on + strand at 3628.478 kb on + strand at 3628.481 kb on + strand at 3628.481 kb on + strand at 3628.481 kb on + strand at 3628.482 kb on - strand at 3628.482 kb on - strand at 3628.565 kb on + strand at 3628.566 kb on - strand at 3628.747 kb on + strand, within Pf1N1B4_3242 at 3628.800 kb on - strand, within Pf1N1B4_3242 at 3628.800 kb on - strand at 3628.803 kb on + strand, within Pf1N1B4_3242 at 3628.803 kb on + strand, within Pf1N1B4_3242 at 3628.803 kb on + strand, within Pf1N1B4_3242 at 3628.803 kb on + strand, within Pf1N1B4_3242 at 3628.803 kb on + strand, within Pf1N1B4_3242 at 3628.803 kb on + strand, within Pf1N1B4_3242 at 3628.804 kb on - strand, within Pf1N1B4_3242 at 3628.804 kb on - strand, within Pf1N1B4_3242 at 3628.948 kb on + strand, within Pf1N1B4_3242 at 3628.949 kb on - strand, within Pf1N1B4_3242 at 3629.174 kb on - strand, within Pf1N1B4_3243 at 3629.174 kb on - strand, within Pf1N1B4_3243 at 3629.182 kb on + strand, within Pf1N1B4_3243 at 3629.235 kb on - strand, within Pf1N1B4_3243 at 3629.237 kb on - strand, within Pf1N1B4_3243 at 3629.271 kb on - strand, within Pf1N1B4_3243
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 3,627,261 + Pf1N1B4_3240 0.78 -0.9 3,627,261 + Pf1N1B4_3240 0.78 -1.0 3,627,262 - Pf1N1B4_3240 0.78 -0.3 3,627,262 - -1.0 3,627,262 - Pf1N1B4_3240 0.78 +0.4 3,627,386 - Pf1N1B4_3240 0.88 +0.4 3,627,396 + Pf1N1B4_3240 0.89 -2.3 3,627,396 + Pf1N1B4_3240 0.89 +0.6 3,627,510 + -0.4 3,627,510 + -0.0 3,627,511 - -2.6 3,627,511 - -1.0 3,627,511 - -0.0 3,627,549 - -1.0 3,627,554 + -0.6 3,627,554 + +0.3 3,627,554 + +0.6 3,627,554 + -0.6 3,627,555 - +1.0 3,627,570 - -0.0 3,627,685 + +2.3 3,627,685 + -0.4 3,627,686 - -0.0 3,627,767 + -2.0 3,627,807 + -1.3 3,627,812 - -0.0 3,628,158 - -0.4 3,628,228 + Pf1N1B4_3241 0.30 -1.6 3,628,273 + Pf1N1B4_3241 0.47 -1.6 3,628,273 + Pf1N1B4_3241 0.47 -1.0 3,628,274 - Pf1N1B4_3241 0.47 -1.0 3,628,277 + Pf1N1B4_3241 0.48 +1.3 3,628,478 + +1.6 3,628,478 + -1.0 3,628,478 + -0.0 3,628,481 + -1.2 3,628,481 + -0.3 3,628,481 + -1.0 3,628,482 - -0.6 3,628,482 - -2.6 3,628,565 + +0.8 3,628,566 - -2.6 3,628,747 + Pf1N1B4_3242 0.36 -1.0 3,628,800 - Pf1N1B4_3242 0.49 -2.0 3,628,800 - -1.0 3,628,803 + Pf1N1B4_3242 0.50 -1.0 3,628,803 + Pf1N1B4_3242 0.50 +0.4 3,628,803 + Pf1N1B4_3242 0.50 -0.4 3,628,803 + Pf1N1B4_3242 0.50 -1.6 3,628,803 + Pf1N1B4_3242 0.50 +2.0 3,628,803 + Pf1N1B4_3242 0.50 -0.7 3,628,804 - Pf1N1B4_3242 0.50 +0.2 3,628,804 - Pf1N1B4_3242 0.50 +0.1 3,628,948 + Pf1N1B4_3242 0.87 +0.2 3,628,949 - Pf1N1B4_3242 0.87 -0.0 3,629,174 - Pf1N1B4_3243 0.14 -2.3 3,629,174 - Pf1N1B4_3243 0.14 -1.0 3,629,182 + Pf1N1B4_3243 0.14 -0.9 3,629,235 - Pf1N1B4_3243 0.19 -1.0 3,629,237 - Pf1N1B4_3243 0.19 -0.0 3,629,271 - Pf1N1B4_3243 0.22 +0.6
Or see this region's nucleotide sequence