Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3217 and Pf1N1B4_3218 are separated by 233 nucleotides Pf1N1B4_3218 and Pf1N1B4_3219 are separated by 450 nucleotides Pf1N1B4_3219 and Pf1N1B4_3220 are separated by 281 nucleotides
Pf1N1B4_3217 - High-affinity branched-chain amino acid transport system permease protein LivH (TC 3.A.1.4.1), at 3,598,465 to 3,599,388
_3217
Pf1N1B4_3218 - High-affinity leucine-specific transport system, periplasmic binding protein LivK (TC 3.A.1.4.1), at 3,599,622 to 3,600,749
_3218
Pf1N1B4_3219 - FIG00955830: hypothetical protein, at 3,601,200 to 3,601,406
_3219
Pf1N1B4_3220 - FIG00953621: hypothetical protein, at 3,601,688 to 3,603,883
_3220
Position (kb)
3599
3600
3601 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 3598.798 kb on - strand, within Pf1N1B4_3217 at 3598.928 kb on + strand, within Pf1N1B4_3217 at 3599.237 kb on + strand, within Pf1N1B4_3217 at 3599.237 kb on + strand at 3599.237 kb on + strand, within Pf1N1B4_3217 at 3599.237 kb on + strand, within Pf1N1B4_3217 at 3599.237 kb on + strand, within Pf1N1B4_3217 at 3599.237 kb on + strand, within Pf1N1B4_3217 at 3599.237 kb on + strand, within Pf1N1B4_3217 at 3599.252 kb on + strand, within Pf1N1B4_3217 at 3599.252 kb on + strand, within Pf1N1B4_3217 at 3599.252 kb on + strand, within Pf1N1B4_3217 at 3599.252 kb on + strand, within Pf1N1B4_3217 at 3599.253 kb on - strand, within Pf1N1B4_3217 at 3599.253 kb on - strand, within Pf1N1B4_3217 at 3599.253 kb on - strand, within Pf1N1B4_3217 at 3599.304 kb on + strand at 3599.304 kb on + strand at 3599.304 kb on + strand at 3599.304 kb on + strand at 3599.305 kb on - strand at 3599.305 kb on - strand at 3599.327 kb on + strand at 3599.327 kb on + strand at 3599.327 kb on + strand at 3599.327 kb on + strand at 3599.327 kb on + strand at 3599.327 kb on + strand at 3599.327 kb on + strand at 3599.327 kb on + strand at 3599.327 kb on + strand at 3599.328 kb on - strand at 3599.328 kb on - strand at 3599.375 kb on + strand at 3599.375 kb on + strand at 3599.375 kb on + strand at 3599.375 kb on + strand at 3599.376 kb on - strand at 3599.376 kb on - strand at 3599.389 kb on + strand at 3599.412 kb on - strand at 3599.412 kb on - strand at 3599.557 kb on + strand at 3599.557 kb on + strand at 3599.557 kb on + strand at 3599.558 kb on - strand at 3599.565 kb on + strand at 3599.597 kb on - strand at 3599.597 kb on - strand at 3599.623 kb on + strand at 3599.624 kb on - strand at 3599.645 kb on + strand at 3599.646 kb on - strand at 3599.754 kb on - strand, within Pf1N1B4_3218 at 3599.754 kb on - strand, within Pf1N1B4_3218 at 3599.828 kb on - strand, within Pf1N1B4_3218 at 3600.077 kb on - strand, within Pf1N1B4_3218 at 3600.195 kb on - strand, within Pf1N1B4_3218 at 3600.221 kb on - strand, within Pf1N1B4_3218 at 3600.277 kb on + strand, within Pf1N1B4_3218 at 3600.277 kb on + strand, within Pf1N1B4_3218 at 3600.277 kb on + strand, within Pf1N1B4_3218 at 3600.277 kb on + strand, within Pf1N1B4_3218 at 3600.277 kb on + strand at 3600.277 kb on + strand, within Pf1N1B4_3218 at 3600.277 kb on + strand, within Pf1N1B4_3218 at 3600.277 kb on + strand, within Pf1N1B4_3218 at 3600.277 kb on + strand, within Pf1N1B4_3218 at 3600.277 kb on + strand, within Pf1N1B4_3218 at 3600.277 kb on + strand, within Pf1N1B4_3218 at 3600.277 kb on + strand, within Pf1N1B4_3218 at 3600.277 kb on + strand, within Pf1N1B4_3218 at 3600.278 kb on - strand, within Pf1N1B4_3218 at 3600.278 kb on - strand, within Pf1N1B4_3218 at 3600.278 kb on - strand, within Pf1N1B4_3218 at 3600.278 kb on - strand, within Pf1N1B4_3218 at 3600.278 kb on - strand, within Pf1N1B4_3218 at 3600.278 kb on - strand, within Pf1N1B4_3218 at 3600.282 kb on - strand, within Pf1N1B4_3218 at 3600.337 kb on + strand, within Pf1N1B4_3218 at 3600.403 kb on + strand, within Pf1N1B4_3218 at 3600.404 kb on - strand, within Pf1N1B4_3218 at 3600.404 kb on - strand, within Pf1N1B4_3218 at 3600.404 kb on - strand, within Pf1N1B4_3218 at 3600.523 kb on + strand, within Pf1N1B4_3218 at 3600.523 kb on + strand, within Pf1N1B4_3218 at 3600.524 kb on - strand, within Pf1N1B4_3218 at 3600.734 kb on + strand at 3600.734 kb on + strand at 3600.735 kb on - strand at 3600.735 kb on - strand at 3600.737 kb on + strand at 3600.744 kb on - strand at 3600.744 kb on - strand at 3600.750 kb on - strand at 3600.763 kb on + strand at 3600.763 kb on + strand at 3600.764 kb on - strand at 3600.771 kb on + strand at 3600.771 kb on + strand at 3600.772 kb on - strand at 3600.772 kb on - strand at 3600.778 kb on + strand at 3600.778 kb on + strand at 3600.778 kb on + strand at 3600.779 kb on - strand at 3600.779 kb on - strand at 3600.779 kb on - strand at 3600.779 kb on - strand at 3600.779 kb on - strand at 3600.779 kb on - strand at 3600.779 kb on - strand at 3600.779 kb on - strand at 3600.779 kb on - strand at 3600.779 kb on - strand at 3600.780 kb on + strand at 3600.781 kb on - strand at 3600.781 kb on - strand at 3600.866 kb on + strand at 3600.964 kb on - strand at 3600.964 kb on - strand at 3600.964 kb on - strand at 3600.972 kb on - strand at 3600.972 kb on - strand at 3600.974 kb on + strand at 3600.975 kb on - strand at 3600.975 kb on - strand at 3600.975 kb on - strand at 3600.975 kb on - strand at 3600.975 kb on - strand at 3600.975 kb on - strand at 3601.064 kb on + strand at 3601.065 kb on - strand at 3601.115 kb on + strand at 3601.115 kb on + strand at 3601.115 kb on + strand at 3601.115 kb on + strand at 3601.115 kb on + strand at 3601.115 kb on + strand at 3601.115 kb on + strand at 3601.116 kb on - strand at 3601.116 kb on - strand at 3601.116 kb on - strand at 3601.116 kb on - strand at 3601.116 kb on - strand at 3601.116 kb on - strand at 3601.116 kb on - strand at 3601.116 kb on - strand at 3601.126 kb on - strand at 3601.126 kb on - strand at 3601.126 kb on - strand at 3601.154 kb on + strand at 3601.154 kb on + strand at 3601.154 kb on + strand at 3601.154 kb on + strand at 3601.154 kb on + strand at 3601.154 kb on + strand at 3601.154 kb on + strand at 3601.154 kb on + strand at 3601.155 kb on - strand at 3601.155 kb on - strand at 3601.155 kb on - strand at 3601.155 kb on - strand at 3601.155 kb on - strand at 3601.155 kb on - strand at 3601.155 kb on - strand at 3601.155 kb on - strand at 3601.155 kb on - strand at 3601.155 kb on - strand at 3601.155 kb on - strand at 3601.155 kb on - strand at 3601.155 kb on - strand at 3601.155 kb on - strand at 3601.158 kb on - strand at 3601.198 kb on + strand at 3601.198 kb on + strand at 3601.199 kb on - strand at 3601.418 kb on + strand at 3601.419 kb on - strand at 3601.519 kb on + strand at 3601.519 kb on + strand at 3601.519 kb on + strand at 3601.540 kb on - strand at 3601.562 kb on + strand at 3601.562 kb on + strand at 3601.562 kb on + strand at 3601.563 kb on - strand at 3601.563 kb on - strand at 3601.563 kb on - strand at 3601.680 kb on + strand at 3601.680 kb on + strand at 3601.680 kb on + strand at 3601.681 kb on - strand at 3601.681 kb on - strand at 3601.681 kb on - strand at 3601.689 kb on + strand at 3601.690 kb on - strand at 3601.712 kb on - strand at 3601.712 kb on - strand at 3601.724 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 3,598,798 - Pf1N1B4_3217 0.36 +0.6 3,598,928 + Pf1N1B4_3217 0.50 -0.0 3,599,237 + Pf1N1B4_3217 0.84 -1.2 3,599,237 + +1.0 3,599,237 + Pf1N1B4_3217 0.84 -2.0 3,599,237 + Pf1N1B4_3217 0.84 -1.0 3,599,237 + Pf1N1B4_3217 0.84 -1.6 3,599,237 + Pf1N1B4_3217 0.84 -1.6 3,599,237 + Pf1N1B4_3217 0.84 -2.8 3,599,252 + Pf1N1B4_3217 0.85 -0.4 3,599,252 + Pf1N1B4_3217 0.85 +0.4 3,599,252 + Pf1N1B4_3217 0.85 -1.2 3,599,252 + Pf1N1B4_3217 0.85 -0.0 3,599,253 - Pf1N1B4_3217 0.85 +2.0 3,599,253 - Pf1N1B4_3217 0.85 -3.2 3,599,253 - Pf1N1B4_3217 0.85 -1.0 3,599,304 + -1.6 3,599,304 + -2.3 3,599,304 + -0.0 3,599,304 + -0.0 3,599,305 - -1.0 3,599,305 - -0.0 3,599,327 + -1.0 3,599,327 + -2.6 3,599,327 + +1.0 3,599,327 + -2.0 3,599,327 + -1.0 3,599,327 + -1.0 3,599,327 + -0.0 3,599,327 + -0.0 3,599,327 + -1.6 3,599,328 - -0.0 3,599,328 - -2.6 3,599,375 + -2.0 3,599,375 + -2.0 3,599,375 + -1.6 3,599,375 + -0.6 3,599,376 - -0.7 3,599,376 - -0.6 3,599,389 + -1.0 3,599,412 - -3.0 3,599,412 - -1.6 3,599,557 + -0.4 3,599,557 + -1.5 3,599,557 + -0.0 3,599,558 - -2.8 3,599,565 + -2.0 3,599,597 - -2.2 3,599,597 - -1.3 3,599,623 + -2.6 3,599,624 - -3.3 3,599,645 + -1.0 3,599,646 - -0.0 3,599,754 - Pf1N1B4_3218 0.12 -1.0 3,599,754 - Pf1N1B4_3218 0.12 -2.0 3,599,828 - Pf1N1B4_3218 0.18 -1.6 3,600,077 - Pf1N1B4_3218 0.40 -1.2 3,600,195 - Pf1N1B4_3218 0.51 -0.0 3,600,221 - Pf1N1B4_3218 0.53 -2.3 3,600,277 + Pf1N1B4_3218 0.58 -1.0 3,600,277 + Pf1N1B4_3218 0.58 -1.0 3,600,277 + Pf1N1B4_3218 0.58 -1.1 3,600,277 + Pf1N1B4_3218 0.58 -1.0 3,600,277 + +1.6 3,600,277 + Pf1N1B4_3218 0.58 -0.0 3,600,277 + Pf1N1B4_3218 0.58 -2.8 3,600,277 + Pf1N1B4_3218 0.58 -0.7 3,600,277 + Pf1N1B4_3218 0.58 -0.4 3,600,277 + Pf1N1B4_3218 0.58 -0.3 3,600,277 + Pf1N1B4_3218 0.58 -1.0 3,600,277 + Pf1N1B4_3218 0.58 -2.3 3,600,277 + Pf1N1B4_3218 0.58 -0.0 3,600,278 - Pf1N1B4_3218 0.58 -1.6 3,600,278 - Pf1N1B4_3218 0.58 -0.0 3,600,278 - Pf1N1B4_3218 0.58 -1.6 3,600,278 - Pf1N1B4_3218 0.58 -1.6 3,600,278 - Pf1N1B4_3218 0.58 +0.6 3,600,278 - Pf1N1B4_3218 0.58 -0.9 3,600,282 - Pf1N1B4_3218 0.59 -1.0 3,600,337 + Pf1N1B4_3218 0.63 -0.0 3,600,403 + Pf1N1B4_3218 0.69 -1.6 3,600,404 - Pf1N1B4_3218 0.69 -1.0 3,600,404 - Pf1N1B4_3218 0.69 -0.5 3,600,404 - Pf1N1B4_3218 0.69 -0.0 3,600,523 + Pf1N1B4_3218 0.80 -0.0 3,600,523 + Pf1N1B4_3218 0.80 -2.0 3,600,524 - Pf1N1B4_3218 0.80 +1.6 3,600,734 + -0.0 3,600,734 + -2.6 3,600,735 - -0.0 3,600,735 - +0.3 3,600,737 + +1.0 3,600,744 - -0.7 3,600,744 - -0.0 3,600,750 - -0.0 3,600,763 + -1.0 3,600,763 + -1.2 3,600,764 - -0.4 3,600,771 + -0.0 3,600,771 + -0.4 3,600,772 - -1.0 3,600,772 - -2.6 3,600,778 + +0.4 3,600,778 + -1.6 3,600,778 + -0.0 3,600,779 - -2.0 3,600,779 - -2.6 3,600,779 - -3.6 3,600,779 - -0.0 3,600,779 - -2.0 3,600,779 - -3.0 3,600,779 - -1.6 3,600,779 - -0.7 3,600,779 - -4.1 3,600,779 - -2.0 3,600,780 + -0.8 3,600,781 - +1.0 3,600,781 - -2.9 3,600,866 + -1.2 3,600,964 - -2.8 3,600,964 - -3.6 3,600,964 - -3.3 3,600,972 - -2.6 3,600,972 - -1.0 3,600,974 + -0.0 3,600,975 - -1.5 3,600,975 - -0.0 3,600,975 - -1.6 3,600,975 - -2.0 3,600,975 - -1.0 3,600,975 - -0.0 3,601,064 + -2.0 3,601,065 - -3.2 3,601,115 + -1.6 3,601,115 + -1.0 3,601,115 + -1.3 3,601,115 + -1.0 3,601,115 + -0.0 3,601,115 + +0.2 3,601,115 + -1.3 3,601,116 - -3.3 3,601,116 - -4.1 3,601,116 - -2.6 3,601,116 - -3.2 3,601,116 - -1.3 3,601,116 - -1.6 3,601,116 - -4.3 3,601,116 - -2.0 3,601,126 - -2.6 3,601,126 - -2.0 3,601,126 - -3.5 3,601,154 + -0.6 3,601,154 + -2.6 3,601,154 + -0.0 3,601,154 + -0.0 3,601,154 + -2.0 3,601,154 + -0.0 3,601,154 + +1.0 3,601,154 + +0.6 3,601,155 - -0.0 3,601,155 - -2.8 3,601,155 - -1.6 3,601,155 - -3.8 3,601,155 - -2.8 3,601,155 - -1.8 3,601,155 - -2.6 3,601,155 - -2.8 3,601,155 - -0.4 3,601,155 - -2.3 3,601,155 - -0.0 3,601,155 - -3.5 3,601,155 - -0.0 3,601,155 - -3.0 3,601,158 - -2.0 3,601,198 + -0.8 3,601,198 + -1.8 3,601,199 - -3.0 3,601,418 + -0.3 3,601,419 - -2.8 3,601,519 + +1.0 3,601,519 + -1.0 3,601,519 + -0.6 3,601,540 - -0.4 3,601,562 + +0.4 3,601,562 + -1.8 3,601,562 + -0.4 3,601,563 - -0.3 3,601,563 - -1.8 3,601,563 - -1.6 3,601,680 + -0.6 3,601,680 + -0.3 3,601,680 + -1.0 3,601,681 - -1.0 3,601,681 - -0.0 3,601,681 - -0.0 3,601,689 + -2.8 3,601,690 - +1.0 3,601,712 - -0.0 3,601,712 - -1.6 3,601,724 - -0.6
Or see this region's nucleotide sequence