Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3214 and Pf1N1B4_3215 are separated by 0 nucleotides Pf1N1B4_3215 and Pf1N1B4_3216 overlap by 4 nucleotides Pf1N1B4_3216 and Pf1N1B4_3217 overlap by 4 nucleotides
Pf1N1B4_3214 - Branched-chain amino acid transport ATP-binding protein LivF (TC 3.A.1.4.1), at 3,595,746 to 3,596,447
_3214
Pf1N1B4_3215 - Branched-chain amino acid transport ATP-binding protein LivG (TC 3.A.1.4.1), at 3,596,448 to 3,597,215
_3215
Pf1N1B4_3216 - Branched-chain amino acid transport system permease protein LivM (TC 3.A.1.4.1), at 3,597,212 to 3,598,468
_3216
Pf1N1B4_3217 - High-affinity branched-chain amino acid transport system permease protein LivH (TC 3.A.1.4.1), at 3,598,465 to 3,599,388
_3217
Position (kb)
3597
3598
3599 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3596.222 kb on + strand at 3596.222 kb on + strand, within Pf1N1B4_3214 at 3596.223 kb on - strand, within Pf1N1B4_3214 at 3596.223 kb on - strand, within Pf1N1B4_3214 at 3596.223 kb on - strand, within Pf1N1B4_3214 at 3596.223 kb on - strand at 3596.269 kb on + strand, within Pf1N1B4_3214 at 3596.269 kb on + strand, within Pf1N1B4_3214 at 3596.270 kb on - strand, within Pf1N1B4_3214 at 3596.416 kb on + strand at 3596.416 kb on + strand at 3596.416 kb on + strand at 3596.417 kb on - strand at 3596.417 kb on - strand at 3596.417 kb on - strand at 3596.449 kb on + strand at 3596.449 kb on + strand at 3596.449 kb on + strand at 3596.449 kb on + strand at 3596.450 kb on - strand at 3596.450 kb on - strand at 3596.450 kb on - strand at 3596.450 kb on - strand at 3596.464 kb on + strand at 3596.464 kb on + strand at 3596.810 kb on - strand, within Pf1N1B4_3215 at 3597.368 kb on + strand at 3597.541 kb on + strand, within Pf1N1B4_3216 at 3597.542 kb on - strand, within Pf1N1B4_3216 at 3597.672 kb on + strand, within Pf1N1B4_3216 at 3597.702 kb on + strand, within Pf1N1B4_3216 at 3597.702 kb on + strand, within Pf1N1B4_3216 at 3597.703 kb on - strand, within Pf1N1B4_3216 at 3597.717 kb on + strand, within Pf1N1B4_3216 at 3597.717 kb on + strand at 3597.717 kb on + strand, within Pf1N1B4_3216 at 3597.717 kb on + strand, within Pf1N1B4_3216 at 3597.717 kb on + strand, within Pf1N1B4_3216 at 3597.755 kb on - strand, within Pf1N1B4_3216 at 3597.755 kb on - strand, within Pf1N1B4_3216 at 3597.885 kb on + strand, within Pf1N1B4_3216 at 3597.985 kb on - strand at 3597.987 kb on + strand, within Pf1N1B4_3216 at 3597.987 kb on + strand, within Pf1N1B4_3216 at 3597.988 kb on - strand, within Pf1N1B4_3216 at 3597.988 kb on - strand, within Pf1N1B4_3216 at 3598.006 kb on - strand, within Pf1N1B4_3216 at 3598.012 kb on - strand, within Pf1N1B4_3216 at 3598.026 kb on + strand, within Pf1N1B4_3216 at 3598.026 kb on + strand at 3598.026 kb on + strand, within Pf1N1B4_3216 at 3598.039 kb on - strand, within Pf1N1B4_3216 at 3598.101 kb on + strand, within Pf1N1B4_3216 at 3598.101 kb on + strand, within Pf1N1B4_3216 at 3598.101 kb on + strand, within Pf1N1B4_3216 at 3598.101 kb on + strand, within Pf1N1B4_3216 at 3598.102 kb on - strand, within Pf1N1B4_3216 at 3598.102 kb on - strand, within Pf1N1B4_3216 at 3598.102 kb on - strand, within Pf1N1B4_3216 at 3598.102 kb on - strand, within Pf1N1B4_3216 at 3598.102 kb on - strand, within Pf1N1B4_3216 at 3598.102 kb on - strand, within Pf1N1B4_3216 at 3598.102 kb on - strand, within Pf1N1B4_3216 at 3598.102 kb on - strand, within Pf1N1B4_3216 at 3598.102 kb on - strand, within Pf1N1B4_3216 at 3598.102 kb on - strand, within Pf1N1B4_3216 at 3598.102 kb on - strand, within Pf1N1B4_3216 at 3598.222 kb on + strand, within Pf1N1B4_3216 at 3598.223 kb on - strand, within Pf1N1B4_3216 at 3598.463 kb on - strand at 3598.463 kb on - strand at 3598.468 kb on + strand at 3598.468 kb on + strand at 3598.468 kb on + strand at 3598.468 kb on + strand at 3598.469 kb on - strand at 3598.469 kb on - strand at 3598.469 kb on - strand at 3598.469 kb on - strand at 3598.557 kb on - strand at 3598.557 kb on - strand at 3598.798 kb on - strand, within Pf1N1B4_3217 at 3598.928 kb on + strand, within Pf1N1B4_3217 at 3599.237 kb on + strand, within Pf1N1B4_3217 at 3599.237 kb on + strand at 3599.237 kb on + strand, within Pf1N1B4_3217 at 3599.237 kb on + strand, within Pf1N1B4_3217 at 3599.237 kb on + strand, within Pf1N1B4_3217 at 3599.237 kb on + strand, within Pf1N1B4_3217 at 3599.237 kb on + strand, within Pf1N1B4_3217 at 3599.252 kb on + strand, within Pf1N1B4_3217 at 3599.252 kb on + strand, within Pf1N1B4_3217 at 3599.252 kb on + strand, within Pf1N1B4_3217 at 3599.252 kb on + strand, within Pf1N1B4_3217 at 3599.253 kb on - strand, within Pf1N1B4_3217 at 3599.253 kb on - strand, within Pf1N1B4_3217 at 3599.253 kb on - strand, within Pf1N1B4_3217 at 3599.304 kb on + strand at 3599.304 kb on + strand at 3599.304 kb on + strand at 3599.304 kb on + strand at 3599.305 kb on - strand at 3599.305 kb on - strand at 3599.327 kb on + strand at 3599.327 kb on + strand at 3599.327 kb on + strand at 3599.327 kb on + strand at 3599.327 kb on + strand at 3599.327 kb on + strand at 3599.327 kb on + strand at 3599.327 kb on + strand at 3599.327 kb on + strand at 3599.328 kb on - strand at 3599.328 kb on - strand at 3599.375 kb on + strand at 3599.375 kb on + strand at 3599.375 kb on + strand at 3599.375 kb on + strand at 3599.376 kb on - strand at 3599.376 kb on - strand at 3599.389 kb on + strand at 3599.412 kb on - strand at 3599.412 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 3,596,222 + +1.0 3,596,222 + Pf1N1B4_3214 0.68 -1.6 3,596,223 - Pf1N1B4_3214 0.68 +1.6 3,596,223 - Pf1N1B4_3214 0.68 +1.0 3,596,223 - Pf1N1B4_3214 0.68 -1.6 3,596,223 - -1.6 3,596,269 + Pf1N1B4_3214 0.75 -1.0 3,596,269 + Pf1N1B4_3214 0.75 -0.4 3,596,270 - Pf1N1B4_3214 0.75 -1.8 3,596,416 + -1.6 3,596,416 + -1.6 3,596,416 + -2.0 3,596,417 - -0.8 3,596,417 - -1.3 3,596,417 - -1.0 3,596,449 + +0.0 3,596,449 + -2.0 3,596,449 + +0.0 3,596,449 + +0.0 3,596,450 - -0.4 3,596,450 - +2.0 3,596,450 - -0.7 3,596,450 - -0.2 3,596,464 + +1.0 3,596,464 + -1.3 3,596,810 - Pf1N1B4_3215 0.47 -0.7 3,597,368 + -0.0 3,597,541 + Pf1N1B4_3216 0.26 -1.3 3,597,542 - Pf1N1B4_3216 0.26 -0.5 3,597,672 + Pf1N1B4_3216 0.37 -0.0 3,597,702 + Pf1N1B4_3216 0.39 -1.0 3,597,702 + Pf1N1B4_3216 0.39 -1.4 3,597,703 - Pf1N1B4_3216 0.39 -1.3 3,597,717 + Pf1N1B4_3216 0.40 -2.0 3,597,717 + -0.0 3,597,717 + Pf1N1B4_3216 0.40 -0.0 3,597,717 + Pf1N1B4_3216 0.40 +1.6 3,597,717 + Pf1N1B4_3216 0.40 -1.0 3,597,755 - Pf1N1B4_3216 0.43 -0.0 3,597,755 - Pf1N1B4_3216 0.43 -0.8 3,597,885 + Pf1N1B4_3216 0.54 -1.4 3,597,985 - -2.6 3,597,987 + Pf1N1B4_3216 0.62 -2.6 3,597,987 + Pf1N1B4_3216 0.62 -0.0 3,597,988 - Pf1N1B4_3216 0.62 -0.0 3,597,988 - Pf1N1B4_3216 0.62 -2.0 3,598,006 - Pf1N1B4_3216 0.63 +0.7 3,598,012 - Pf1N1B4_3216 0.64 -1.0 3,598,026 + Pf1N1B4_3216 0.65 +1.6 3,598,026 + -0.0 3,598,026 + Pf1N1B4_3216 0.65 -1.6 3,598,039 - Pf1N1B4_3216 0.66 -1.7 3,598,101 + Pf1N1B4_3216 0.71 -0.0 3,598,101 + Pf1N1B4_3216 0.71 -2.3 3,598,101 + Pf1N1B4_3216 0.71 -0.0 3,598,101 + Pf1N1B4_3216 0.71 +1.0 3,598,102 - Pf1N1B4_3216 0.71 -0.6 3,598,102 - Pf1N1B4_3216 0.71 -2.3 3,598,102 - Pf1N1B4_3216 0.71 -0.0 3,598,102 - Pf1N1B4_3216 0.71 -1.4 3,598,102 - Pf1N1B4_3216 0.71 +1.0 3,598,102 - Pf1N1B4_3216 0.71 -1.0 3,598,102 - Pf1N1B4_3216 0.71 -0.4 3,598,102 - Pf1N1B4_3216 0.71 -0.4 3,598,102 - Pf1N1B4_3216 0.71 -1.7 3,598,102 - Pf1N1B4_3216 0.71 -1.4 3,598,102 - Pf1N1B4_3216 0.71 -0.6 3,598,222 + Pf1N1B4_3216 0.80 -1.3 3,598,223 - Pf1N1B4_3216 0.80 -3.7 3,598,463 - -1.0 3,598,463 - -1.0 3,598,468 + -1.0 3,598,468 + -0.7 3,598,468 + +0.3 3,598,468 + -0.2 3,598,469 - -1.6 3,598,469 - -1.0 3,598,469 - -0.0 3,598,469 - -0.6 3,598,557 - -2.3 3,598,557 - -0.6 3,598,798 - Pf1N1B4_3217 0.36 +0.6 3,598,928 + Pf1N1B4_3217 0.50 -0.0 3,599,237 + Pf1N1B4_3217 0.84 -1.2 3,599,237 + +1.0 3,599,237 + Pf1N1B4_3217 0.84 -2.0 3,599,237 + Pf1N1B4_3217 0.84 -1.0 3,599,237 + Pf1N1B4_3217 0.84 -1.6 3,599,237 + Pf1N1B4_3217 0.84 -1.6 3,599,237 + Pf1N1B4_3217 0.84 -2.8 3,599,252 + Pf1N1B4_3217 0.85 -0.4 3,599,252 + Pf1N1B4_3217 0.85 +0.4 3,599,252 + Pf1N1B4_3217 0.85 -1.2 3,599,252 + Pf1N1B4_3217 0.85 -0.0 3,599,253 - Pf1N1B4_3217 0.85 +2.0 3,599,253 - Pf1N1B4_3217 0.85 -3.2 3,599,253 - Pf1N1B4_3217 0.85 -1.0 3,599,304 + -1.6 3,599,304 + -2.3 3,599,304 + -0.0 3,599,304 + -0.0 3,599,305 - -1.0 3,599,305 - -0.0 3,599,327 + -1.0 3,599,327 + -2.6 3,599,327 + +1.0 3,599,327 + -2.0 3,599,327 + -1.0 3,599,327 + -1.0 3,599,327 + -0.0 3,599,327 + -0.0 3,599,327 + -1.6 3,599,328 - -0.0 3,599,328 - -2.6 3,599,375 + -2.0 3,599,375 + -2.0 3,599,375 + -1.6 3,599,375 + -0.6 3,599,376 - -0.7 3,599,376 - -0.6 3,599,389 + -1.0 3,599,412 - -3.0 3,599,412 - -1.6
Or see this region's nucleotide sequence