Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3151 and Pf1N1B4_3152 overlap by 4 nucleotides Pf1N1B4_3152 and Pf1N1B4_3153 are separated by 37 nucleotides Pf1N1B4_3153 and Pf1N1B4_3154 overlap by 39 nucleotides Pf1N1B4_3154 and Pf1N1B4_3155 are separated by 98 nucleotides
Pf1N1B4_3151 - Diacylglycerol kinase (EC 2.7.1.107), at 3,531,448 to 3,531,810
_3151
Pf1N1B4_3152 - LysR family transcriptional regulator PA3398, at 3,531,807 to 3,532,733
_3152
Pf1N1B4_3153 - hypothetical protein, at 3,532,771 to 3,532,893
_3153
Pf1N1B4_3154 - Ferredoxin--NADP(+) reductase (EC 1.18.1.2), at 3,532,855 to 3,533,634
_3154
Pf1N1B4_3155 - COG1720: Uncharacterized conserved protein, at 3,533,733 to 3,534,437
_3155
Position (kb)
3532
3533 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3531.808 kb on + strand at 3531.808 kb on + strand at 3531.905 kb on - strand at 3531.905 kb on - strand at 3531.928 kb on + strand, within Pf1N1B4_3152 at 3531.928 kb on + strand, within Pf1N1B4_3152 at 3531.928 kb on + strand, within Pf1N1B4_3152 at 3531.928 kb on + strand, within Pf1N1B4_3152 at 3531.928 kb on + strand, within Pf1N1B4_3152 at 3531.929 kb on - strand, within Pf1N1B4_3152 at 3531.929 kb on - strand, within Pf1N1B4_3152 at 3532.300 kb on - strand, within Pf1N1B4_3152 at 3532.319 kb on - strand, within Pf1N1B4_3152 at 3532.424 kb on - strand, within Pf1N1B4_3152 at 3532.424 kb on - strand, within Pf1N1B4_3152 at 3532.424 kb on - strand, within Pf1N1B4_3152 at 3532.424 kb on - strand, within Pf1N1B4_3152 at 3532.424 kb on - strand, within Pf1N1B4_3152 at 3532.424 kb on - strand, within Pf1N1B4_3152 at 3532.424 kb on - strand, within Pf1N1B4_3152 at 3532.424 kb on - strand, within Pf1N1B4_3152 at 3532.424 kb on - strand, within Pf1N1B4_3152 at 3532.424 kb on - strand, within Pf1N1B4_3152 at 3532.431 kb on + strand, within Pf1N1B4_3152 at 3532.704 kb on + strand at 3532.724 kb on + strand at 3532.724 kb on + strand at 3532.724 kb on + strand at 3532.779 kb on + strand at 3532.794 kb on + strand, within Pf1N1B4_3153 at 3532.794 kb on + strand, within Pf1N1B4_3153 at 3532.794 kb on + strand, within Pf1N1B4_3153 at 3532.794 kb on + strand at 3532.794 kb on + strand, within Pf1N1B4_3153 at 3532.794 kb on + strand, within Pf1N1B4_3153 at 3532.794 kb on + strand at 3532.794 kb on + strand, within Pf1N1B4_3153 at 3533.632 kb on + strand at 3533.632 kb on + strand at 3533.632 kb on + strand at 3533.632 kb on + strand at 3533.632 kb on + strand at 3533.632 kb on + strand at 3533.632 kb on + strand at 3533.632 kb on + strand at 3533.633 kb on - strand at 3533.633 kb on - strand at 3533.633 kb on - strand at 3533.633 kb on - strand at 3533.633 kb on - strand at 3533.734 kb on + strand at 3533.735 kb on - strand at 3533.735 kb on - strand at 3533.802 kb on + strand at 3533.802 kb on + strand at 3533.802 kb on + strand at 3533.802 kb on + strand at 3533.802 kb on + strand at 3533.802 kb on + strand at 3533.803 kb on - strand at 3533.803 kb on - strand at 3533.803 kb on - strand at 3533.803 kb on - strand at 3533.803 kb on - strand at 3533.831 kb on - strand, within Pf1N1B4_3155 at 3533.847 kb on - strand, within Pf1N1B4_3155 at 3533.847 kb on - strand, within Pf1N1B4_3155
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 3,531,808 + -1.6 3,531,808 + -0.4 3,531,905 - +0.0 3,531,905 - -1.6 3,531,928 + Pf1N1B4_3152 0.13 +0.0 3,531,928 + Pf1N1B4_3152 0.13 +0.0 3,531,928 + Pf1N1B4_3152 0.13 -0.3 3,531,928 + Pf1N1B4_3152 0.13 -1.3 3,531,928 + Pf1N1B4_3152 0.13 +0.0 3,531,929 - Pf1N1B4_3152 0.13 -1.4 3,531,929 - Pf1N1B4_3152 0.13 -1.0 3,532,300 - Pf1N1B4_3152 0.53 -0.6 3,532,319 - Pf1N1B4_3152 0.55 -1.8 3,532,424 - Pf1N1B4_3152 0.67 -1.0 3,532,424 - Pf1N1B4_3152 0.67 -2.0 3,532,424 - Pf1N1B4_3152 0.67 -1.3 3,532,424 - Pf1N1B4_3152 0.67 -2.1 3,532,424 - Pf1N1B4_3152 0.67 -1.0 3,532,424 - Pf1N1B4_3152 0.67 -1.6 3,532,424 - Pf1N1B4_3152 0.67 -1.6 3,532,424 - Pf1N1B4_3152 0.67 -2.3 3,532,424 - Pf1N1B4_3152 0.67 -3.1 3,532,424 - Pf1N1B4_3152 0.67 -2.6 3,532,431 + Pf1N1B4_3152 0.67 -1.9 3,532,704 + -0.1 3,532,724 + +0.0 3,532,724 + -1.0 3,532,724 + -1.0 3,532,779 + -1.8 3,532,794 + Pf1N1B4_3153 0.19 -0.7 3,532,794 + Pf1N1B4_3153 0.19 +0.0 3,532,794 + Pf1N1B4_3153 0.19 -2.0 3,532,794 + -2.0 3,532,794 + Pf1N1B4_3153 0.19 -1.6 3,532,794 + Pf1N1B4_3153 0.19 -2.0 3,532,794 + -1.0 3,532,794 + Pf1N1B4_3153 0.19 +0.0 3,533,632 + -1.0 3,533,632 + +0.0 3,533,632 + -0.5 3,533,632 + -0.2 3,533,632 + -0.4 3,533,632 + -1.6 3,533,632 + +1.6 3,533,632 + -0.4 3,533,633 - +0.0 3,533,633 - +0.8 3,533,633 - -0.6 3,533,633 - +0.0 3,533,633 - +0.0 3,533,734 + +0.0 3,533,735 - -2.3 3,533,735 - -0.3 3,533,802 + -1.3 3,533,802 + -0.4 3,533,802 + +0.0 3,533,802 + +1.6 3,533,802 + +0.0 3,533,802 + +0.0 3,533,803 - +0.4 3,533,803 - -1.0 3,533,803 - +1.0 3,533,803 - +1.0 3,533,803 - -2.0 3,533,831 - Pf1N1B4_3155 0.14 -2.0 3,533,847 - Pf1N1B4_3155 0.16 -1.8 3,533,847 - Pf1N1B4_3155 0.16 -0.8
Or see this region's nucleotide sequence