Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3114 and Pf1N1B4_3115 are separated by 548 nucleotides Pf1N1B4_3115 and Pf1N1B4_3116 are separated by 485 nucleotides Pf1N1B4_3116 and Pf1N1B4_3117 are separated by 142 nucleotides
Pf1N1B4_3114 - hypothetical protein, at 3,500,678 to 3,500,806
_3114
Pf1N1B4_3115 - RNA polymerase sigma factor RpoS, at 3,501,355 to 3,502,362
_3115
Pf1N1B4_3116 - 4Fe-4S ferredoxin, iron-sulfur binding, at 3,502,848 to 3,503,171
_3116
Pf1N1B4_3117 - DNA mismatch repair protein MutS, at 3,503,314 to 3,505,893
_3117
Position (kb)
3501
3502
3503 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 3501.276 kb on - strand at 3501.348 kb on + strand at 3501.350 kb on + strand at 3501.350 kb on + strand at 3501.351 kb on - strand at 3501.367 kb on - strand at 3501.502 kb on - strand, within Pf1N1B4_3115 at 3501.514 kb on + strand, within Pf1N1B4_3115 at 3501.514 kb on + strand, within Pf1N1B4_3115 at 3501.514 kb on + strand, within Pf1N1B4_3115 at 3501.514 kb on + strand, within Pf1N1B4_3115 at 3501.514 kb on + strand, within Pf1N1B4_3115 at 3501.514 kb on + strand, within Pf1N1B4_3115 at 3501.514 kb on + strand, within Pf1N1B4_3115 at 3501.514 kb on + strand, within Pf1N1B4_3115 at 3501.515 kb on - strand, within Pf1N1B4_3115 at 3501.515 kb on - strand at 3501.515 kb on - strand, within Pf1N1B4_3115 at 3501.515 kb on - strand at 3501.515 kb on - strand, within Pf1N1B4_3115 at 3501.515 kb on - strand, within Pf1N1B4_3115 at 3501.515 kb on - strand, within Pf1N1B4_3115 at 3501.515 kb on - strand, within Pf1N1B4_3115 at 3501.523 kb on + strand, within Pf1N1B4_3115 at 3501.554 kb on - strand, within Pf1N1B4_3115 at 3501.554 kb on - strand at 3501.554 kb on - strand, within Pf1N1B4_3115 at 3501.554 kb on - strand, within Pf1N1B4_3115 at 3501.697 kb on + strand, within Pf1N1B4_3115 at 3501.764 kb on + strand, within Pf1N1B4_3115 at 3501.764 kb on + strand, within Pf1N1B4_3115 at 3501.764 kb on + strand at 3501.805 kb on + strand, within Pf1N1B4_3115 at 3501.806 kb on - strand, within Pf1N1B4_3115 at 3501.806 kb on - strand, within Pf1N1B4_3115 at 3501.864 kb on + strand, within Pf1N1B4_3115 at 3501.904 kb on + strand, within Pf1N1B4_3115 at 3501.904 kb on + strand, within Pf1N1B4_3115 at 3501.904 kb on + strand at 3501.904 kb on + strand at 3501.905 kb on - strand, within Pf1N1B4_3115 at 3501.905 kb on - strand at 3502.010 kb on + strand, within Pf1N1B4_3115 at 3502.010 kb on + strand, within Pf1N1B4_3115 at 3502.011 kb on - strand, within Pf1N1B4_3115 at 3502.066 kb on - strand, within Pf1N1B4_3115 at 3502.066 kb on - strand at 3502.244 kb on + strand, within Pf1N1B4_3115 at 3502.244 kb on + strand, within Pf1N1B4_3115 at 3502.244 kb on + strand, within Pf1N1B4_3115 at 3502.244 kb on + strand, within Pf1N1B4_3115 at 3502.245 kb on - strand, within Pf1N1B4_3115 at 3502.423 kb on + strand at 3502.423 kb on + strand at 3502.423 kb on + strand at 3502.424 kb on - strand at 3502.424 kb on - strand at 3502.424 kb on - strand at 3502.425 kb on + strand at 3502.425 kb on + strand at 3502.425 kb on + strand at 3502.425 kb on + strand at 3502.425 kb on + strand at 3502.426 kb on - strand at 3502.426 kb on - strand at 3502.426 kb on - strand at 3502.463 kb on + strand at 3502.463 kb on + strand at 3502.476 kb on + strand at 3502.476 kb on + strand at 3502.476 kb on + strand at 3502.477 kb on - strand at 3502.489 kb on - strand at 3502.499 kb on - strand at 3502.516 kb on - strand at 3502.537 kb on + strand at 3502.636 kb on - strand at 3502.650 kb on + strand at 3502.651 kb on - strand at 3502.653 kb on + strand at 3502.654 kb on - strand at 3503.241 kb on - strand at 3503.292 kb on + strand at 3503.293 kb on - strand at 3503.315 kb on + strand at 3503.315 kb on + strand at 3503.315 kb on + strand at 3503.315 kb on + strand at 3503.315 kb on + strand at 3503.316 kb on - strand at 3503.329 kb on + strand at 3503.329 kb on + strand at 3503.330 kb on - strand at 3503.330 kb on - strand at 3503.334 kb on + strand at 3503.334 kb on + strand at 3503.334 kb on + strand at 3503.334 kb on + strand at 3503.334 kb on + strand at 3503.334 kb on + strand at 3503.334 kb on + strand at 3503.334 kb on + strand at 3503.334 kb on + strand at 3503.334 kb on + strand at 3503.334 kb on + strand at 3503.334 kb on + strand at 3503.334 kb on + strand at 3503.334 kb on + strand at 3503.334 kb on + strand at 3503.334 kb on + strand at 3503.334 kb on + strand at 3503.334 kb on + strand at 3503.334 kb on + strand at 3503.334 kb on + strand at 3503.334 kb on + strand at 3503.334 kb on + strand at 3503.334 kb on + strand at 3503.335 kb on - strand at 3503.335 kb on - strand at 3503.335 kb on - strand at 3503.335 kb on - strand at 3503.335 kb on - strand at 3503.335 kb on - strand at 3503.335 kb on - strand at 3503.335 kb on - strand at 3503.335 kb on - strand at 3503.335 kb on - strand at 3503.335 kb on - strand at 3503.336 kb on + strand at 3503.336 kb on + strand at 3503.336 kb on + strand at 3503.336 kb on + strand at 3503.336 kb on + strand at 3503.336 kb on + strand at 3503.337 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 3,501,276 - +2.5 3,501,348 + +3.1 3,501,350 + +1.1 3,501,350 + +2.6 3,501,351 - +0.6 3,501,367 - +1.6 3,501,502 - Pf1N1B4_3115 0.15 +1.6 3,501,514 + Pf1N1B4_3115 0.16 +2.8 3,501,514 + Pf1N1B4_3115 0.16 +2.2 3,501,514 + Pf1N1B4_3115 0.16 +2.0 3,501,514 + Pf1N1B4_3115 0.16 +2.2 3,501,514 + Pf1N1B4_3115 0.16 +2.4 3,501,514 + Pf1N1B4_3115 0.16 +1.5 3,501,514 + Pf1N1B4_3115 0.16 +2.6 3,501,514 + Pf1N1B4_3115 0.16 +1.9 3,501,515 - Pf1N1B4_3115 0.16 +1.3 3,501,515 - +2.2 3,501,515 - Pf1N1B4_3115 0.16 +2.9 3,501,515 - +1.6 3,501,515 - Pf1N1B4_3115 0.16 +1.3 3,501,515 - Pf1N1B4_3115 0.16 +1.3 3,501,515 - Pf1N1B4_3115 0.16 +0.0 3,501,515 - Pf1N1B4_3115 0.16 +1.8 3,501,523 + Pf1N1B4_3115 0.17 +2.6 3,501,554 - Pf1N1B4_3115 0.20 +3.3 3,501,554 - +1.8 3,501,554 - Pf1N1B4_3115 0.20 +1.0 3,501,554 - Pf1N1B4_3115 0.20 +4.3 3,501,697 + Pf1N1B4_3115 0.34 +0.0 3,501,764 + Pf1N1B4_3115 0.41 +1.7 3,501,764 + Pf1N1B4_3115 0.41 +3.4 3,501,764 + +2.0 3,501,805 + Pf1N1B4_3115 0.45 +1.5 3,501,806 - Pf1N1B4_3115 0.45 +2.2 3,501,806 - Pf1N1B4_3115 0.45 +2.4 3,501,864 + Pf1N1B4_3115 0.50 +2.3 3,501,904 + Pf1N1B4_3115 0.54 +0.6 3,501,904 + Pf1N1B4_3115 0.54 +1.0 3,501,904 + +1.8 3,501,904 + +0.6 3,501,905 - Pf1N1B4_3115 0.55 +0.8 3,501,905 - +0.6 3,502,010 + Pf1N1B4_3115 0.65 +1.5 3,502,010 + Pf1N1B4_3115 0.65 +0.0 3,502,011 - Pf1N1B4_3115 0.65 +2.2 3,502,066 - Pf1N1B4_3115 0.71 +2.0 3,502,066 - +0.8 3,502,244 + Pf1N1B4_3115 0.88 +2.6 3,502,244 + Pf1N1B4_3115 0.88 -0.2 3,502,244 + Pf1N1B4_3115 0.88 +2.0 3,502,244 + Pf1N1B4_3115 0.88 +1.2 3,502,245 - Pf1N1B4_3115 0.88 +2.7 3,502,423 + +1.1 3,502,423 + -0.4 3,502,423 + -0.1 3,502,424 - +1.3 3,502,424 - +0.0 3,502,424 - +0.0 3,502,425 + +0.4 3,502,425 + -1.5 3,502,425 + +0.0 3,502,425 + +0.0 3,502,425 + +2.5 3,502,426 - -0.4 3,502,426 - -0.7 3,502,426 - -0.7 3,502,463 + -1.0 3,502,463 + -0.5 3,502,476 + -0.5 3,502,476 + +0.0 3,502,476 + -1.5 3,502,477 - +1.0 3,502,489 - -2.8 3,502,499 - -1.1 3,502,516 - -0.4 3,502,537 + +1.8 3,502,636 - +0.4 3,502,650 + -0.4 3,502,651 - +1.0 3,502,653 + -1.0 3,502,654 - -0.7 3,503,241 - +2.6 3,503,292 + +0.0 3,503,293 - -1.3 3,503,315 + -0.3 3,503,315 + -3.0 3,503,315 + -1.0 3,503,315 + -1.3 3,503,315 + -1.0 3,503,316 - +1.0 3,503,329 + +0.2 3,503,329 + +0.3 3,503,330 - +0.0 3,503,330 - +0.6 3,503,334 + -0.3 3,503,334 + -0.7 3,503,334 + +1.6 3,503,334 + -0.5 3,503,334 + -0.5 3,503,334 + -0.7 3,503,334 + -0.5 3,503,334 + +0.6 3,503,334 + -1.0 3,503,334 + -1.0 3,503,334 + -1.4 3,503,334 + -2.0 3,503,334 + -1.3 3,503,334 + -1.6 3,503,334 + +0.4 3,503,334 + -1.0 3,503,334 + +0.0 3,503,334 + -1.5 3,503,334 + +0.0 3,503,334 + -0.5 3,503,334 + -2.0 3,503,334 + -0.4 3,503,334 + -1.3 3,503,335 - -0.3 3,503,335 - -1.5 3,503,335 - -1.7 3,503,335 - +0.0 3,503,335 - -0.3 3,503,335 - -1.8 3,503,335 - -0.5 3,503,335 - +0.2 3,503,335 - -1.0 3,503,335 - -1.3 3,503,335 - -0.4 3,503,336 + -0.3 3,503,336 + -0.2 3,503,336 + -0.7 3,503,336 + -1.0 3,503,336 + -0.7 3,503,336 + -0.7 3,503,337 - +1.6
Or see this region's nucleotide sequence