Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_3078

Experiment: L-Isoleucine (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_3077 and Pf1N1B4_3078 are separated by 33 nucleotidesPf1N1B4_3078 and Pf1N1B4_3079 are separated by 265 nucleotides Pf1N1B4_3077 - 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase (EC 2.3.1.117), at 3,459,950 to 3,460,984 _3077 Pf1N1B4_3078 - FIG138056: a glutathione-dependent thiol reductase, at 3,461,018 to 3,461,380 _3078 Pf1N1B4_3079 - Transcription-repair coupling factor (superfamily II helicase), at 3,461,646 to 3,462,377 _3079 Position (kb) 3461 3462Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 3460.997 kb on - strandat 3461.020 kb on - strandat 3461.020 kb on - strandat 3461.020 kb on - strandat 3461.038 kb on - strandat 3461.038 kb on - strandat 3461.038 kb on - strandat 3461.038 kb on - strandat 3461.042 kb on - strandat 3461.042 kb on - strandat 3461.042 kb on - strandat 3461.042 kb on - strandat 3461.042 kb on - strandat 3461.042 kb on - strandat 3461.042 kb on - strandat 3461.269 kb on - strand, within Pf1N1B4_3078at 3461.269 kb on - strand, within Pf1N1B4_3078at 3461.269 kb on - strand, within Pf1N1B4_3078at 3461.269 kb on - strand, within Pf1N1B4_3078at 3461.284 kb on - strand, within Pf1N1B4_3078at 3461.284 kb on - strand, within Pf1N1B4_3078at 3461.410 kb on + strandat 3461.411 kb on - strandat 3461.411 kb on - strandat 3461.411 kb on - strandat 3461.425 kb on + strandat 3461.426 kb on - strandat 3461.426 kb on - strandat 3461.473 kb on + strandat 3461.473 kb on + strandat 3461.559 kb on - strandat 3461.559 kb on - strandat 3461.559 kb on - strandat 3461.591 kb on + strandat 3461.591 kb on + strandat 3461.591 kb on + strandat 3461.592 kb on - strandat 3461.592 kb on - strandat 3461.592 kb on - strandat 3461.691 kb on - strandat 3461.777 kb on - strand, within Pf1N1B4_3079at 3461.777 kb on - strand, within Pf1N1B4_3079at 3461.784 kb on - strand, within Pf1N1B4_3079at 3461.785 kb on + strand, within Pf1N1B4_3079at 3461.785 kb on + strand, within Pf1N1B4_3079at 3461.785 kb on + strand, within Pf1N1B4_3079at 3461.785 kb on + strand, within Pf1N1B4_3079at 3461.786 kb on - strand, within Pf1N1B4_3079at 3461.786 kb on - strand, within Pf1N1B4_3079at 3461.786 kb on - strand, within Pf1N1B4_3079at 3461.879 kb on - strand, within Pf1N1B4_3079at 3461.879 kb on - strand, within Pf1N1B4_3079at 3461.879 kb on - strand, within Pf1N1B4_3079at 3461.891 kb on + strand, within Pf1N1B4_3079at 3462.065 kb on + strand, within Pf1N1B4_3079at 3462.065 kb on + strand, within Pf1N1B4_3079at 3462.065 kb on + strand, within Pf1N1B4_3079at 3462.066 kb on - strand, within Pf1N1B4_3079at 3462.066 kb on - strand, within Pf1N1B4_3079at 3462.066 kb on - strand, within Pf1N1B4_3079at 3462.066 kb on - strand, within Pf1N1B4_3079at 3462.066 kb on - strand, within Pf1N1B4_3079at 3462.066 kb on - strand, within Pf1N1B4_3079at 3462.066 kb on - strand, within Pf1N1B4_3079at 3462.066 kb on - strand, within Pf1N1B4_3079at 3462.069 kb on - strand, within Pf1N1B4_3079at 3462.134 kb on + strand, within Pf1N1B4_3079at 3462.136 kb on + strand, within Pf1N1B4_3079at 3462.136 kb on + strand, within Pf1N1B4_3079at 3462.136 kb on + strand, within Pf1N1B4_3079at 3462.136 kb on + strand, within Pf1N1B4_3079at 3462.136 kb on + strand, within Pf1N1B4_3079at 3462.137 kb on - strand, within Pf1N1B4_3079at 3462.200 kb on + strand, within Pf1N1B4_3079at 3462.312 kb on - strandat 3462.322 kb on + strandat 3462.378 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Isoleucine (C)
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3,460,997 - -1.1
3,461,020 - -0.5
3,461,020 - -0.3
3,461,020 - -1.2
3,461,038 - -0.3
3,461,038 - -1.4
3,461,038 - +1.0
3,461,038 - +0.0
3,461,042 - -0.3
3,461,042 - -1.5
3,461,042 - -1.6
3,461,042 - +1.0
3,461,042 - +1.0
3,461,042 - -1.0
3,461,042 - +2.0
3,461,269 - Pf1N1B4_3078 0.69 -2.0
3,461,269 - Pf1N1B4_3078 0.69 -2.0
3,461,269 - Pf1N1B4_3078 0.69 -1.5
3,461,269 - Pf1N1B4_3078 0.69 +0.0
3,461,284 - Pf1N1B4_3078 0.73 -1.0
3,461,284 - Pf1N1B4_3078 0.73 +0.0
3,461,410 + +1.0
3,461,411 - -2.0
3,461,411 - +0.0
3,461,411 - -0.5
3,461,425 + -2.0
3,461,426 - -1.4
3,461,426 - -0.9
3,461,473 + -1.5
3,461,473 + -0.4
3,461,559 - -2.5
3,461,559 - -0.7
3,461,559 - -0.5
3,461,591 + -0.7
3,461,591 + +1.0
3,461,591 + +0.2
3,461,592 - +0.5
3,461,592 - -1.7
3,461,592 - -3.3
3,461,691 - +0.3
3,461,777 - Pf1N1B4_3079 0.18 -2.0
3,461,777 - Pf1N1B4_3079 0.18 +1.0
3,461,784 - Pf1N1B4_3079 0.19 -1.0
3,461,785 + Pf1N1B4_3079 0.19 +0.5
3,461,785 + Pf1N1B4_3079 0.19 +0.5
3,461,785 + Pf1N1B4_3079 0.19 +0.0
3,461,785 + Pf1N1B4_3079 0.19 -0.5
3,461,786 - Pf1N1B4_3079 0.19 -1.3
3,461,786 - Pf1N1B4_3079 0.19 -0.3
3,461,786 - Pf1N1B4_3079 0.19 +0.6
3,461,879 - Pf1N1B4_3079 0.32 +0.0
3,461,879 - Pf1N1B4_3079 0.32 +2.4
3,461,879 - Pf1N1B4_3079 0.32 +0.6
3,461,891 + Pf1N1B4_3079 0.33 -1.5
3,462,065 + Pf1N1B4_3079 0.57 -1.4
3,462,065 + Pf1N1B4_3079 0.57 +1.8
3,462,065 + Pf1N1B4_3079 0.57 +0.2
3,462,066 - Pf1N1B4_3079 0.57 -0.7
3,462,066 - Pf1N1B4_3079 0.57 +1.6
3,462,066 - Pf1N1B4_3079 0.57 -0.5
3,462,066 - Pf1N1B4_3079 0.57 +1.0
3,462,066 - Pf1N1B4_3079 0.57 +0.8
3,462,066 - Pf1N1B4_3079 0.57 +1.6
3,462,066 - Pf1N1B4_3079 0.57 -0.2
3,462,066 - Pf1N1B4_3079 0.57 -0.1
3,462,069 - Pf1N1B4_3079 0.58 +1.4
3,462,134 + Pf1N1B4_3079 0.67 -0.2
3,462,136 + Pf1N1B4_3079 0.67 -1.7
3,462,136 + Pf1N1B4_3079 0.67 +0.2
3,462,136 + Pf1N1B4_3079 0.67 +0.6
3,462,136 + Pf1N1B4_3079 0.67 -2.0
3,462,136 + Pf1N1B4_3079 0.67 +0.0
3,462,137 - Pf1N1B4_3079 0.67 -1.0
3,462,200 + Pf1N1B4_3079 0.76 -1.0
3,462,312 - -0.4
3,462,322 + +0.0
3,462,378 + -1.0

Or see this region's nucleotide sequence