Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3077 and Pf1N1B4_3078 are separated by 33 nucleotides Pf1N1B4_3078 and Pf1N1B4_3079 are separated by 265 nucleotides
Pf1N1B4_3077 - 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase (EC 2.3.1.117), at 3,459,950 to 3,460,984
_3077
Pf1N1B4_3078 - FIG138056: a glutathione-dependent thiol reductase, at 3,461,018 to 3,461,380
_3078
Pf1N1B4_3079 - Transcription-repair coupling factor (superfamily II helicase), at 3,461,646 to 3,462,377
_3079
Position (kb)
3461
3462 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3460.997 kb on - strand at 3461.020 kb on - strand at 3461.020 kb on - strand at 3461.020 kb on - strand at 3461.038 kb on - strand at 3461.038 kb on - strand at 3461.038 kb on - strand at 3461.038 kb on - strand at 3461.042 kb on - strand at 3461.042 kb on - strand at 3461.042 kb on - strand at 3461.042 kb on - strand at 3461.042 kb on - strand at 3461.042 kb on - strand at 3461.042 kb on - strand at 3461.269 kb on - strand, within Pf1N1B4_3078 at 3461.269 kb on - strand, within Pf1N1B4_3078 at 3461.269 kb on - strand, within Pf1N1B4_3078 at 3461.269 kb on - strand, within Pf1N1B4_3078 at 3461.284 kb on - strand, within Pf1N1B4_3078 at 3461.284 kb on - strand, within Pf1N1B4_3078 at 3461.410 kb on + strand at 3461.411 kb on - strand at 3461.411 kb on - strand at 3461.411 kb on - strand at 3461.425 kb on + strand at 3461.426 kb on - strand at 3461.426 kb on - strand at 3461.473 kb on + strand at 3461.473 kb on + strand at 3461.559 kb on - strand at 3461.559 kb on - strand at 3461.559 kb on - strand at 3461.591 kb on + strand at 3461.591 kb on + strand at 3461.591 kb on + strand at 3461.592 kb on - strand at 3461.592 kb on - strand at 3461.592 kb on - strand at 3461.691 kb on - strand at 3461.777 kb on - strand, within Pf1N1B4_3079 at 3461.777 kb on - strand, within Pf1N1B4_3079 at 3461.784 kb on - strand, within Pf1N1B4_3079 at 3461.785 kb on + strand, within Pf1N1B4_3079 at 3461.785 kb on + strand, within Pf1N1B4_3079 at 3461.785 kb on + strand, within Pf1N1B4_3079 at 3461.785 kb on + strand, within Pf1N1B4_3079 at 3461.786 kb on - strand, within Pf1N1B4_3079 at 3461.786 kb on - strand, within Pf1N1B4_3079 at 3461.786 kb on - strand, within Pf1N1B4_3079 at 3461.879 kb on - strand, within Pf1N1B4_3079 at 3461.879 kb on - strand, within Pf1N1B4_3079 at 3461.879 kb on - strand, within Pf1N1B4_3079 at 3461.891 kb on + strand, within Pf1N1B4_3079 at 3462.065 kb on + strand, within Pf1N1B4_3079 at 3462.065 kb on + strand, within Pf1N1B4_3079 at 3462.065 kb on + strand, within Pf1N1B4_3079 at 3462.066 kb on - strand, within Pf1N1B4_3079 at 3462.066 kb on - strand, within Pf1N1B4_3079 at 3462.066 kb on - strand, within Pf1N1B4_3079 at 3462.066 kb on - strand, within Pf1N1B4_3079 at 3462.066 kb on - strand, within Pf1N1B4_3079 at 3462.066 kb on - strand, within Pf1N1B4_3079 at 3462.066 kb on - strand, within Pf1N1B4_3079 at 3462.066 kb on - strand, within Pf1N1B4_3079 at 3462.069 kb on - strand, within Pf1N1B4_3079 at 3462.134 kb on + strand, within Pf1N1B4_3079 at 3462.136 kb on + strand, within Pf1N1B4_3079 at 3462.136 kb on + strand, within Pf1N1B4_3079 at 3462.136 kb on + strand, within Pf1N1B4_3079 at 3462.136 kb on + strand, within Pf1N1B4_3079 at 3462.136 kb on + strand, within Pf1N1B4_3079 at 3462.137 kb on - strand, within Pf1N1B4_3079 at 3462.200 kb on + strand, within Pf1N1B4_3079 at 3462.312 kb on - strand at 3462.322 kb on + strand at 3462.378 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 3,460,997 - -1.1 3,461,020 - -0.5 3,461,020 - -0.3 3,461,020 - -1.2 3,461,038 - -0.3 3,461,038 - -1.4 3,461,038 - +1.0 3,461,038 - +0.0 3,461,042 - -0.3 3,461,042 - -1.5 3,461,042 - -1.6 3,461,042 - +1.0 3,461,042 - +1.0 3,461,042 - -1.0 3,461,042 - +2.0 3,461,269 - Pf1N1B4_3078 0.69 -2.0 3,461,269 - Pf1N1B4_3078 0.69 -2.0 3,461,269 - Pf1N1B4_3078 0.69 -1.5 3,461,269 - Pf1N1B4_3078 0.69 +0.0 3,461,284 - Pf1N1B4_3078 0.73 -1.0 3,461,284 - Pf1N1B4_3078 0.73 +0.0 3,461,410 + +1.0 3,461,411 - -2.0 3,461,411 - +0.0 3,461,411 - -0.5 3,461,425 + -2.0 3,461,426 - -1.4 3,461,426 - -0.9 3,461,473 + -1.5 3,461,473 + -0.4 3,461,559 - -2.5 3,461,559 - -0.7 3,461,559 - -0.5 3,461,591 + -0.7 3,461,591 + +1.0 3,461,591 + +0.2 3,461,592 - +0.5 3,461,592 - -1.7 3,461,592 - -3.3 3,461,691 - +0.3 3,461,777 - Pf1N1B4_3079 0.18 -2.0 3,461,777 - Pf1N1B4_3079 0.18 +1.0 3,461,784 - Pf1N1B4_3079 0.19 -1.0 3,461,785 + Pf1N1B4_3079 0.19 +0.5 3,461,785 + Pf1N1B4_3079 0.19 +0.5 3,461,785 + Pf1N1B4_3079 0.19 +0.0 3,461,785 + Pf1N1B4_3079 0.19 -0.5 3,461,786 - Pf1N1B4_3079 0.19 -1.3 3,461,786 - Pf1N1B4_3079 0.19 -0.3 3,461,786 - Pf1N1B4_3079 0.19 +0.6 3,461,879 - Pf1N1B4_3079 0.32 +0.0 3,461,879 - Pf1N1B4_3079 0.32 +2.4 3,461,879 - Pf1N1B4_3079 0.32 +0.6 3,461,891 + Pf1N1B4_3079 0.33 -1.5 3,462,065 + Pf1N1B4_3079 0.57 -1.4 3,462,065 + Pf1N1B4_3079 0.57 +1.8 3,462,065 + Pf1N1B4_3079 0.57 +0.2 3,462,066 - Pf1N1B4_3079 0.57 -0.7 3,462,066 - Pf1N1B4_3079 0.57 +1.6 3,462,066 - Pf1N1B4_3079 0.57 -0.5 3,462,066 - Pf1N1B4_3079 0.57 +1.0 3,462,066 - Pf1N1B4_3079 0.57 +0.8 3,462,066 - Pf1N1B4_3079 0.57 +1.6 3,462,066 - Pf1N1B4_3079 0.57 -0.2 3,462,066 - Pf1N1B4_3079 0.57 -0.1 3,462,069 - Pf1N1B4_3079 0.58 +1.4 3,462,134 + Pf1N1B4_3079 0.67 -0.2 3,462,136 + Pf1N1B4_3079 0.67 -1.7 3,462,136 + Pf1N1B4_3079 0.67 +0.2 3,462,136 + Pf1N1B4_3079 0.67 +0.6 3,462,136 + Pf1N1B4_3079 0.67 -2.0 3,462,136 + Pf1N1B4_3079 0.67 +0.0 3,462,137 - Pf1N1B4_3079 0.67 -1.0 3,462,200 + Pf1N1B4_3079 0.76 -1.0 3,462,312 - -0.4 3,462,322 + +0.0 3,462,378 + -1.0
Or see this region's nucleotide sequence