Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3037 and Pf1N1B4_3038 overlap by 4 nucleotides Pf1N1B4_3038 and Pf1N1B4_3039 are separated by 48 nucleotides
Pf1N1B4_3037 - Sensor histidine kinase/response regulator, at 3,417,671 to 3,419,959
_3037
Pf1N1B4_3038 - Chemotaxis response regulator protein-glutamate methylesterase CheB (EC 3.1.1.61), at 3,419,956 to 3,420,966
_3038
Pf1N1B4_3039 - diguanylate cyclase (GGDEF domain) with PAS/PAC sensor, at 3,421,015 to 3,422,016
_3039
Position (kb)
3419
3420
3421 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1
2 at 3419.023 kb on + strand, within Pf1N1B4_3037 at 3419.023 kb on + strand, within Pf1N1B4_3037 at 3419.024 kb on - strand, within Pf1N1B4_3037 at 3419.024 kb on - strand, within Pf1N1B4_3037 at 3419.024 kb on - strand, within Pf1N1B4_3037 at 3419.024 kb on - strand, within Pf1N1B4_3037 at 3419.024 kb on - strand, within Pf1N1B4_3037 at 3419.024 kb on - strand, within Pf1N1B4_3037 at 3419.024 kb on - strand, within Pf1N1B4_3037 at 3419.153 kb on + strand, within Pf1N1B4_3037 at 3419.153 kb on + strand, within Pf1N1B4_3037 at 3419.153 kb on + strand at 3419.154 kb on - strand, within Pf1N1B4_3037 at 3419.207 kb on - strand, within Pf1N1B4_3037 at 3419.207 kb on - strand, within Pf1N1B4_3037 at 3419.207 kb on - strand, within Pf1N1B4_3037 at 3419.355 kb on - strand, within Pf1N1B4_3037 at 3419.401 kb on - strand, within Pf1N1B4_3037 at 3419.657 kb on + strand, within Pf1N1B4_3037 at 3419.657 kb on + strand, within Pf1N1B4_3037 at 3419.828 kb on + strand at 3419.828 kb on + strand at 3419.829 kb on - strand at 3419.829 kb on - strand at 3419.829 kb on - strand at 3419.829 kb on - strand at 3419.829 kb on - strand at 3419.829 kb on - strand at 3419.879 kb on + strand at 3419.879 kb on + strand at 3419.880 kb on - strand at 3419.880 kb on - strand at 3419.882 kb on + strand at 3419.882 kb on + strand at 3419.882 kb on + strand at 3419.882 kb on + strand at 3419.882 kb on + strand at 3419.883 kb on - strand at 3419.883 kb on - strand at 3419.883 kb on - strand at 3420.088 kb on + strand at 3420.088 kb on + strand, within Pf1N1B4_3038 at 3420.088 kb on + strand, within Pf1N1B4_3038 at 3420.088 kb on + strand, within Pf1N1B4_3038 at 3420.089 kb on - strand, within Pf1N1B4_3038 at 3420.700 kb on + strand at 3420.700 kb on + strand, within Pf1N1B4_3038 at 3420.701 kb on - strand, within Pf1N1B4_3038 at 3420.701 kb on - strand, within Pf1N1B4_3038 at 3420.701 kb on - strand, within Pf1N1B4_3038 at 3420.743 kb on - strand at 3420.743 kb on - strand, within Pf1N1B4_3038 at 3420.780 kb on + strand, within Pf1N1B4_3038 at 3420.807 kb on + strand, within Pf1N1B4_3038 at 3420.808 kb on - strand, within Pf1N1B4_3038 at 3420.829 kb on + strand, within Pf1N1B4_3038 at 3420.830 kb on - strand, within Pf1N1B4_3038 at 3420.869 kb on - strand at 3420.932 kb on + strand at 3420.974 kb on + strand at 3420.998 kb on + strand at 3420.999 kb on - strand at 3421.025 kb on + strand at 3421.025 kb on + strand at 3421.025 kb on + strand at 3421.025 kb on + strand at 3421.025 kb on + strand at 3421.026 kb on - strand at 3421.026 kb on - strand at 3421.026 kb on - strand at 3421.189 kb on - strand, within Pf1N1B4_3039 at 3421.189 kb on - strand, within Pf1N1B4_3039 at 3421.263 kb on + strand, within Pf1N1B4_3039 at 3421.284 kb on + strand, within Pf1N1B4_3039 at 3421.360 kb on + strand, within Pf1N1B4_3039 at 3421.361 kb on - strand, within Pf1N1B4_3039 at 3421.361 kb on - strand at 3421.361 kb on - strand, within Pf1N1B4_3039 at 3421.423 kb on + strand, within Pf1N1B4_3039 at 3421.423 kb on + strand, within Pf1N1B4_3039 at 3421.423 kb on + strand, within Pf1N1B4_3039 at 3421.423 kb on + strand, within Pf1N1B4_3039 at 3421.423 kb on + strand at 3421.423 kb on + strand, within Pf1N1B4_3039 at 3421.423 kb on + strand, within Pf1N1B4_3039 at 3421.424 kb on - strand, within Pf1N1B4_3039 at 3421.424 kb on - strand, within Pf1N1B4_3039 at 3421.424 kb on - strand, within Pf1N1B4_3039 at 3421.424 kb on - strand, within Pf1N1B4_3039 at 3421.424 kb on - strand, within Pf1N1B4_3039 at 3421.567 kb on + strand, within Pf1N1B4_3039 at 3421.567 kb on + strand, within Pf1N1B4_3039 at 3421.567 kb on + strand, within Pf1N1B4_3039 at 3421.568 kb on - strand, within Pf1N1B4_3039 at 3421.639 kb on + strand, within Pf1N1B4_3039 at 3421.639 kb on + strand, within Pf1N1B4_3039 at 3421.639 kb on + strand, within Pf1N1B4_3039 at 3421.640 kb on - strand, within Pf1N1B4_3039 at 3421.640 kb on - strand, within Pf1N1B4_3039 at 3421.640 kb on - strand, within Pf1N1B4_3039
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 3,419,023 + Pf1N1B4_3037 0.59 +0.7 3,419,023 + Pf1N1B4_3037 0.59 +1.1 3,419,024 - Pf1N1B4_3037 0.59 +1.1 3,419,024 - Pf1N1B4_3037 0.59 -0.4 3,419,024 - Pf1N1B4_3037 0.59 -0.5 3,419,024 - Pf1N1B4_3037 0.59 -0.5 3,419,024 - Pf1N1B4_3037 0.59 -1.9 3,419,024 - Pf1N1B4_3037 0.59 +1.1 3,419,024 - Pf1N1B4_3037 0.59 +0.6 3,419,153 + Pf1N1B4_3037 0.65 -0.4 3,419,153 + Pf1N1B4_3037 0.65 -1.2 3,419,153 + -0.5 3,419,154 - Pf1N1B4_3037 0.65 -0.9 3,419,207 - Pf1N1B4_3037 0.67 -1.9 3,419,207 - Pf1N1B4_3037 0.67 +2.4 3,419,207 - Pf1N1B4_3037 0.67 -0.9 3,419,355 - Pf1N1B4_3037 0.74 -0.8 3,419,401 - Pf1N1B4_3037 0.76 -0.9 3,419,657 + Pf1N1B4_3037 0.87 -1.2 3,419,657 + Pf1N1B4_3037 0.87 +0.8 3,419,828 + +0.3 3,419,828 + -0.9 3,419,829 - -0.5 3,419,829 - +0.1 3,419,829 - -1.5 3,419,829 - +0.5 3,419,829 - +0.3 3,419,829 - -0.6 3,419,879 + +0.3 3,419,879 + -0.9 3,419,880 - +0.1 3,419,880 - -0.9 3,419,882 + -0.3 3,419,882 + -1.5 3,419,882 + -0.7 3,419,882 + -0.3 3,419,882 + +0.1 3,419,883 - +1.4 3,419,883 - -0.7 3,419,883 - -0.6 3,420,088 + -0.9 3,420,088 + Pf1N1B4_3038 0.13 -1.9 3,420,088 + Pf1N1B4_3038 0.13 -1.9 3,420,088 + Pf1N1B4_3038 0.13 -0.8 3,420,089 - Pf1N1B4_3038 0.13 -2.9 3,420,700 + +0.1 3,420,700 + Pf1N1B4_3038 0.74 -6.6 3,420,701 - Pf1N1B4_3038 0.74 +0.7 3,420,701 - Pf1N1B4_3038 0.74 -0.9 3,420,701 - Pf1N1B4_3038 0.74 -2.8 3,420,743 - -0.9 3,420,743 - Pf1N1B4_3038 0.78 -2.7 3,420,780 + Pf1N1B4_3038 0.82 -2.7 3,420,807 + Pf1N1B4_3038 0.84 -3.9 3,420,808 - Pf1N1B4_3038 0.84 -1.5 3,420,829 + Pf1N1B4_3038 0.86 -2.3 3,420,830 - Pf1N1B4_3038 0.86 -1.5 3,420,869 - -0.9 3,420,932 + -1.9 3,420,974 + -0.9 3,420,998 + -0.3 3,420,999 - -0.4 3,421,025 + -1.3 3,421,025 + -1.3 3,421,025 + +0.1 3,421,025 + +0.1 3,421,025 + +1.1 3,421,026 - +0.4 3,421,026 - -1.5 3,421,026 - +2.1 3,421,189 - Pf1N1B4_3039 0.17 -0.7 3,421,189 - Pf1N1B4_3039 0.17 +0.2 3,421,263 + Pf1N1B4_3039 0.25 -0.9 3,421,284 + Pf1N1B4_3039 0.27 -1.5 3,421,360 + Pf1N1B4_3039 0.34 -1.2 3,421,361 - Pf1N1B4_3039 0.35 +0.1 3,421,361 - -0.5 3,421,361 - Pf1N1B4_3039 0.35 +1.7 3,421,423 + Pf1N1B4_3039 0.41 +0.3 3,421,423 + Pf1N1B4_3039 0.41 -1.5 3,421,423 + Pf1N1B4_3039 0.41 -0.3 3,421,423 + Pf1N1B4_3039 0.41 -0.6 3,421,423 + -0.5 3,421,423 + Pf1N1B4_3039 0.41 +1.7 3,421,423 + Pf1N1B4_3039 0.41 -0.9 3,421,424 - Pf1N1B4_3039 0.41 -0.3 3,421,424 - Pf1N1B4_3039 0.41 -0.4 3,421,424 - Pf1N1B4_3039 0.41 -0.3 3,421,424 - Pf1N1B4_3039 0.41 +0.7 3,421,424 - Pf1N1B4_3039 0.41 +0.6 3,421,567 + Pf1N1B4_3039 0.55 +0.5 3,421,567 + Pf1N1B4_3039 0.55 -0.5 3,421,567 + Pf1N1B4_3039 0.55 +0.8 3,421,568 - Pf1N1B4_3039 0.55 -0.3 3,421,639 + Pf1N1B4_3039 0.62 +0.7 3,421,639 + Pf1N1B4_3039 0.62 +0.7 3,421,639 + Pf1N1B4_3039 0.62 +0.8 3,421,640 - Pf1N1B4_3039 0.62 +1.4 3,421,640 - Pf1N1B4_3039 0.62 -1.5 3,421,640 - Pf1N1B4_3039 0.62 +0.8
Or see this region's nucleotide sequence