Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2853 and Pf1N1B4_2854 overlap by 4 nucleotides Pf1N1B4_2854 and Pf1N1B4_2855 are separated by 371 nucleotides Pf1N1B4_2855 and Pf1N1B4_2856 are separated by 3 nucleotides
Pf1N1B4_2853 - Pantoate--beta-alanine ligase (EC 6.3.2.1), at 3,200,225 to 3,201,085
_2853
Pf1N1B4_2854 - 3-methyl-2-oxobutanoate hydroxymethyltransferase (EC 2.1.2.11), at 3,201,082 to 3,201,882
_2854
Pf1N1B4_2855 - 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine pyrophosphokinase (EC 2.7.6.3), at 3,202,254 to 3,202,739
_2855
Pf1N1B4_2856 - Poly(A) polymerase (EC 2.7.7.19), at 3,202,743 to 3,204,143
_2856
Position (kb)
3201
3202 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3200.093 kb on + strand at 3200.093 kb on + strand at 3200.093 kb on + strand at 3200.093 kb on + strand at 3200.093 kb on + strand at 3200.093 kb on + strand at 3200.093 kb on + strand at 3200.094 kb on - strand at 3200.094 kb on - strand at 3200.149 kb on + strand at 3200.149 kb on + strand at 3200.152 kb on + strand at 3200.152 kb on + strand at 3200.153 kb on - strand at 3200.219 kb on + strand at 3200.226 kb on + strand at 3200.226 kb on + strand at 3200.362 kb on - strand, within Pf1N1B4_2853 at 3200.441 kb on - strand, within Pf1N1B4_2853 at 3200.472 kb on + strand, within Pf1N1B4_2853 at 3200.675 kb on + strand, within Pf1N1B4_2853 at 3201.140 kb on + strand at 3201.140 kb on + strand at 3201.140 kb on + strand at 3201.140 kb on + strand at 3201.141 kb on - strand at 3201.141 kb on - strand at 3201.141 kb on - strand at 3201.141 kb on - strand at 3201.141 kb on - strand at 3201.141 kb on - strand at 3201.141 kb on - strand at 3201.141 kb on - strand at 3201.214 kb on - strand, within Pf1N1B4_2854 at 3201.432 kb on + strand, within Pf1N1B4_2854 at 3201.433 kb on - strand, within Pf1N1B4_2854 at 3201.433 kb on - strand, within Pf1N1B4_2854 at 3201.433 kb on - strand, within Pf1N1B4_2854 at 3201.433 kb on - strand, within Pf1N1B4_2854 at 3201.433 kb on - strand, within Pf1N1B4_2854 at 3201.433 kb on - strand, within Pf1N1B4_2854 at 3201.433 kb on - strand, within Pf1N1B4_2854 at 3201.614 kb on + strand, within Pf1N1B4_2854 at 3201.684 kb on - strand, within Pf1N1B4_2854 at 3201.809 kb on + strand at 3201.809 kb on + strand at 3201.810 kb on - strand at 3201.810 kb on - strand at 3201.810 kb on - strand at 3201.810 kb on - strand at 3201.810 kb on - strand at 3201.810 kb on - strand at 3201.897 kb on + strand at 3201.897 kb on + strand at 3201.897 kb on + strand at 3201.897 kb on + strand at 3201.915 kb on + strand at 3201.915 kb on + strand at 3201.916 kb on - strand at 3201.945 kb on + strand at 3201.945 kb on + strand at 3201.945 kb on + strand at 3201.945 kb on + strand at 3201.945 kb on + strand at 3201.945 kb on + strand at 3201.945 kb on + strand at 3201.945 kb on + strand at 3201.945 kb on + strand at 3201.945 kb on + strand at 3201.945 kb on + strand at 3201.945 kb on + strand at 3201.946 kb on - strand at 3201.946 kb on - strand at 3201.947 kb on + strand at 3201.947 kb on + strand at 3201.947 kb on + strand at 3201.948 kb on - strand at 3201.976 kb on + strand at 3201.976 kb on + strand at 3201.976 kb on + strand at 3201.976 kb on + strand at 3201.976 kb on + strand at 3201.976 kb on + strand at 3201.976 kb on + strand at 3201.976 kb on + strand at 3201.976 kb on + strand at 3201.976 kb on + strand at 3201.976 kb on + strand at 3201.976 kb on + strand at 3201.976 kb on + strand at 3201.976 kb on + strand at 3201.976 kb on + strand at 3201.977 kb on - strand at 3201.977 kb on - strand at 3201.977 kb on - strand at 3202.026 kb on + strand at 3202.026 kb on + strand at 3202.026 kb on + strand at 3202.026 kb on + strand at 3202.026 kb on + strand at 3202.026 kb on + strand at 3202.026 kb on + strand at 3202.026 kb on + strand at 3202.026 kb on + strand at 3202.026 kb on + strand at 3202.026 kb on + strand at 3202.026 kb on + strand at 3202.026 kb on + strand at 3202.027 kb on - strand at 3202.027 kb on - strand at 3202.028 kb on + strand at 3202.028 kb on + strand at 3202.028 kb on + strand at 3202.028 kb on + strand at 3202.028 kb on + strand at 3202.028 kb on + strand at 3202.028 kb on + strand at 3202.029 kb on - strand at 3202.066 kb on + strand at 3202.077 kb on + strand at 3202.099 kb on + strand at 3202.100 kb on - strand at 3202.131 kb on + strand at 3202.207 kb on + strand at 3202.207 kb on + strand at 3202.207 kb on + strand at 3202.207 kb on + strand at 3202.207 kb on + strand at 3202.727 kb on - strand at 3202.727 kb on - strand at 3202.727 kb on - strand at 3202.727 kb on - strand at 3202.727 kb on - strand at 3202.745 kb on - strand at 3202.754 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 3,200,093 + -0.8 3,200,093 + -1.8 3,200,093 + +0.2 3,200,093 + +0.2 3,200,093 + -0.8 3,200,093 + -1.4 3,200,093 + -1.4 3,200,094 - +0.9 3,200,094 - -1.3 3,200,149 + -1.0 3,200,149 + -2.4 3,200,152 + +0.9 3,200,152 + +0.0 3,200,153 - -0.2 3,200,219 + -1.0 3,200,226 + -0.8 3,200,226 + +0.3 3,200,362 - Pf1N1B4_2853 0.16 -1.6 3,200,441 - Pf1N1B4_2853 0.25 +0.2 3,200,472 + Pf1N1B4_2853 0.29 +0.8 3,200,675 + Pf1N1B4_2853 0.52 -0.4 3,201,140 + -2.4 3,201,140 + -1.8 3,201,140 + +0.7 3,201,140 + -0.8 3,201,141 - -0.6 3,201,141 - -1.4 3,201,141 - -0.8 3,201,141 - -1.2 3,201,141 - +0.2 3,201,141 - +1.2 3,201,141 - +0.2 3,201,141 - -0.8 3,201,214 - Pf1N1B4_2854 0.16 -1.6 3,201,432 + Pf1N1B4_2854 0.44 +0.2 3,201,433 - Pf1N1B4_2854 0.44 -2.0 3,201,433 - Pf1N1B4_2854 0.44 +0.2 3,201,433 - Pf1N1B4_2854 0.44 -1.4 3,201,433 - Pf1N1B4_2854 0.44 -0.8 3,201,433 - Pf1N1B4_2854 0.44 -1.1 3,201,433 - Pf1N1B4_2854 0.44 -0.8 3,201,433 - Pf1N1B4_2854 0.44 +1.2 3,201,614 + Pf1N1B4_2854 0.66 -0.6 3,201,684 - Pf1N1B4_2854 0.75 -1.4 3,201,809 + -1.8 3,201,809 + -2.4 3,201,810 - -0.6 3,201,810 - +0.2 3,201,810 - -0.4 3,201,810 - -0.8 3,201,810 - -1.5 3,201,810 - -0.3 3,201,897 + -0.8 3,201,897 + +0.2 3,201,897 + -1.4 3,201,897 + +0.8 3,201,915 + -0.4 3,201,915 + -1.1 3,201,916 - +1.2 3,201,945 + +0.2 3,201,945 + -0.2 3,201,945 + -0.8 3,201,945 + -2.8 3,201,945 + -1.4 3,201,945 + -1.4 3,201,945 + -2.6 3,201,945 + -0.8 3,201,945 + -1.4 3,201,945 + -1.4 3,201,945 + -1.7 3,201,945 + -1.4 3,201,946 - -0.8 3,201,946 - -0.9 3,201,947 + -1.1 3,201,947 + +0.6 3,201,947 + -1.8 3,201,948 - -1.6 3,201,976 + -1.4 3,201,976 + -0.8 3,201,976 + -1.1 3,201,976 + -0.8 3,201,976 + -0.0 3,201,976 + -1.4 3,201,976 + -1.8 3,201,976 + -0.8 3,201,976 + -1.0 3,201,976 + +0.2 3,201,976 + -1.5 3,201,976 + -0.8 3,201,976 + -1.1 3,201,976 + -1.1 3,201,976 + -2.1 3,201,977 - -0.0 3,201,977 - -0.2 3,201,977 - +0.2 3,202,026 + -1.4 3,202,026 + -1.3 3,202,026 + -1.8 3,202,026 + +1.0 3,202,026 + -0.4 3,202,026 + +0.4 3,202,026 + +0.2 3,202,026 + -1.8 3,202,026 + -0.6 3,202,026 + -1.6 3,202,026 + -1.1 3,202,026 + -1.4 3,202,026 + -0.2 3,202,027 - +0.6 3,202,027 - -0.3 3,202,028 + -0.2 3,202,028 + -1.4 3,202,028 + +0.2 3,202,028 + -2.6 3,202,028 + -0.8 3,202,028 + -1.3 3,202,028 + +1.2 3,202,029 - -0.8 3,202,066 + +0.2 3,202,077 + -1.4 3,202,099 + +0.8 3,202,100 - +0.9 3,202,131 + -0.8 3,202,207 + -1.8 3,202,207 + +0.2 3,202,207 + -0.6 3,202,207 + +1.2 3,202,207 + -0.3 3,202,727 - -0.8 3,202,727 - +0.8 3,202,727 - +0.2 3,202,727 - +0.2 3,202,727 - -1.4 3,202,745 - -0.8 3,202,754 + +1.2
Or see this region's nucleotide sequence