Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2737 and Pf1N1B4_2738 are separated by 244 nucleotides Pf1N1B4_2738 and Pf1N1B4_2739 are separated by 90 nucleotides Pf1N1B4_2739 and Pf1N1B4_2740 are separated by 105 nucleotides
Pf1N1B4_2737 - NAD(FAD)-utilizing dehydrogenases, at 3,075,733 to 3,077,010
_2737
Pf1N1B4_2738 - Deacetylases, including yeast histone deacetylase and acetoin utilization protein, at 3,077,255 to 3,078,175
_2738
Pf1N1B4_2739 - acetyltransferase, GNAT family, at 3,078,266 to 3,078,835
_2739
Pf1N1B4_2740 - Acyl-CoA thioesterase II (EC 3.1.2.-), at 3,078,941 to 3,079,810
_2740
Position (kb)
3077
3078
3079 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3076.399 kb on + strand, within Pf1N1B4_2737 at 3076.400 kb on - strand, within Pf1N1B4_2737 at 3076.400 kb on - strand, within Pf1N1B4_2737 at 3076.400 kb on - strand, within Pf1N1B4_2737 at 3076.576 kb on - strand, within Pf1N1B4_2737 at 3076.579 kb on + strand, within Pf1N1B4_2737 at 3076.579 kb on + strand, within Pf1N1B4_2737 at 3076.579 kb on + strand, within Pf1N1B4_2737 at 3076.579 kb on + strand, within Pf1N1B4_2737 at 3076.579 kb on + strand, within Pf1N1B4_2737 at 3076.579 kb on + strand, within Pf1N1B4_2737 at 3076.579 kb on + strand, within Pf1N1B4_2737 at 3076.580 kb on - strand, within Pf1N1B4_2737 at 3076.580 kb on - strand, within Pf1N1B4_2737 at 3076.580 kb on - strand, within Pf1N1B4_2737 at 3076.580 kb on - strand, within Pf1N1B4_2737 at 3076.729 kb on + strand, within Pf1N1B4_2737 at 3076.763 kb on + strand, within Pf1N1B4_2737 at 3076.763 kb on + strand, within Pf1N1B4_2737 at 3076.764 kb on - strand, within Pf1N1B4_2737 at 3076.764 kb on - strand, within Pf1N1B4_2737 at 3076.862 kb on + strand at 3076.863 kb on - strand, within Pf1N1B4_2737 at 3077.013 kb on + strand at 3077.013 kb on + strand at 3077.013 kb on + strand at 3077.013 kb on + strand at 3077.013 kb on + strand at 3077.014 kb on - strand at 3077.086 kb on + strand at 3077.086 kb on + strand at 3077.087 kb on - strand at 3077.087 kb on - strand at 3077.173 kb on + strand at 3077.174 kb on - strand at 3077.174 kb on - strand at 3077.252 kb on - strand at 3077.487 kb on + strand, within Pf1N1B4_2738 at 3077.487 kb on + strand, within Pf1N1B4_2738 at 3077.487 kb on + strand, within Pf1N1B4_2738 at 3077.488 kb on - strand, within Pf1N1B4_2738 at 3077.488 kb on - strand, within Pf1N1B4_2738 at 3077.509 kb on - strand, within Pf1N1B4_2738 at 3077.523 kb on + strand, within Pf1N1B4_2738 at 3077.523 kb on + strand, within Pf1N1B4_2738 at 3077.523 kb on + strand, within Pf1N1B4_2738 at 3077.524 kb on - strand, within Pf1N1B4_2738 at 3077.524 kb on - strand, within Pf1N1B4_2738 at 3077.524 kb on - strand, within Pf1N1B4_2738 at 3077.553 kb on + strand, within Pf1N1B4_2738 at 3077.553 kb on + strand, within Pf1N1B4_2738 at 3077.554 kb on - strand, within Pf1N1B4_2738 at 3077.751 kb on + strand, within Pf1N1B4_2738 at 3077.751 kb on + strand, within Pf1N1B4_2738 at 3077.805 kb on + strand, within Pf1N1B4_2738 at 3077.805 kb on + strand, within Pf1N1B4_2738 at 3077.805 kb on + strand, within Pf1N1B4_2738 at 3077.806 kb on - strand, within Pf1N1B4_2738 at 3077.806 kb on - strand, within Pf1N1B4_2738 at 3077.976 kb on + strand, within Pf1N1B4_2738 at 3077.976 kb on + strand, within Pf1N1B4_2738 at 3077.976 kb on + strand, within Pf1N1B4_2738 at 3077.976 kb on + strand, within Pf1N1B4_2738 at 3077.976 kb on + strand, within Pf1N1B4_2738 at 3077.977 kb on - strand, within Pf1N1B4_2738 at 3077.977 kb on - strand, within Pf1N1B4_2738 at 3077.977 kb on - strand, within Pf1N1B4_2738 at 3077.977 kb on - strand, within Pf1N1B4_2738 at 3077.987 kb on - strand, within Pf1N1B4_2738 at 3077.988 kb on + strand, within Pf1N1B4_2738 at 3077.988 kb on + strand, within Pf1N1B4_2738 at 3077.988 kb on + strand, within Pf1N1B4_2738 at 3077.988 kb on + strand, within Pf1N1B4_2738 at 3077.988 kb on + strand at 3077.988 kb on + strand, within Pf1N1B4_2738 at 3077.988 kb on + strand, within Pf1N1B4_2738 at 3077.988 kb on + strand, within Pf1N1B4_2738 at 3077.988 kb on + strand, within Pf1N1B4_2738 at 3077.988 kb on + strand, within Pf1N1B4_2738 at 3077.988 kb on + strand, within Pf1N1B4_2738 at 3077.988 kb on + strand, within Pf1N1B4_2738 at 3077.988 kb on + strand, within Pf1N1B4_2738 at 3077.988 kb on + strand, within Pf1N1B4_2738 at 3077.989 kb on - strand, within Pf1N1B4_2738 at 3077.989 kb on - strand, within Pf1N1B4_2738 at 3078.144 kb on + strand at 3078.145 kb on - strand at 3078.145 kb on - strand at 3078.156 kb on + strand at 3078.156 kb on + strand at 3078.157 kb on - strand at 3078.157 kb on - strand at 3078.157 kb on - strand at 3078.184 kb on + strand at 3078.184 kb on + strand at 3078.184 kb on + strand at 3078.184 kb on + strand at 3078.185 kb on - strand at 3078.185 kb on - strand at 3078.185 kb on - strand at 3078.186 kb on + strand at 3078.186 kb on + strand at 3078.186 kb on + strand at 3078.200 kb on + strand at 3078.200 kb on + strand at 3078.200 kb on + strand at 3078.220 kb on + strand at 3078.220 kb on + strand at 3078.221 kb on - strand at 3078.231 kb on + strand at 3078.231 kb on + strand at 3078.231 kb on + strand at 3078.231 kb on + strand at 3078.231 kb on + strand at 3078.232 kb on - strand at 3078.232 kb on - strand at 3078.232 kb on - strand at 3078.232 kb on - strand at 3078.538 kb on + strand, within Pf1N1B4_2739 at 3078.539 kb on - strand, within Pf1N1B4_2739 at 3078.593 kb on + strand, within Pf1N1B4_2739 at 3078.791 kb on + strand at 3078.792 kb on - strand at 3078.792 kb on - strand at 3078.833 kb on + strand at 3078.851 kb on + strand at 3078.851 kb on + strand at 3078.851 kb on + strand at 3078.851 kb on + strand at 3078.852 kb on - strand at 3078.852 kb on - strand at 3078.852 kb on - strand at 3078.852 kb on - strand at 3078.852 kb on - strand at 3078.879 kb on + strand at 3079.057 kb on - strand, within Pf1N1B4_2740 at 3079.127 kb on + strand, within Pf1N1B4_2740 at 3079.128 kb on - strand, within Pf1N1B4_2740 at 3079.128 kb on - strand, within Pf1N1B4_2740 at 3079.166 kb on + strand, within Pf1N1B4_2740
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 3,076,399 + Pf1N1B4_2737 0.52 -1.3 3,076,400 - Pf1N1B4_2737 0.52 -0.4 3,076,400 - Pf1N1B4_2737 0.52 -2.1 3,076,400 - Pf1N1B4_2737 0.52 +0.2 3,076,576 - Pf1N1B4_2737 0.66 +0.4 3,076,579 + Pf1N1B4_2737 0.66 -2.1 3,076,579 + Pf1N1B4_2737 0.66 -0.1 3,076,579 + Pf1N1B4_2737 0.66 -0.2 3,076,579 + Pf1N1B4_2737 0.66 +0.8 3,076,579 + Pf1N1B4_2737 0.66 +0.2 3,076,579 + Pf1N1B4_2737 0.66 -2.3 3,076,579 + Pf1N1B4_2737 0.66 +0.2 3,076,580 - Pf1N1B4_2737 0.66 -0.3 3,076,580 - Pf1N1B4_2737 0.66 -1.3 3,076,580 - Pf1N1B4_2737 0.66 -0.8 3,076,580 - Pf1N1B4_2737 0.66 +0.2 3,076,729 + Pf1N1B4_2737 0.78 +1.2 3,076,763 + Pf1N1B4_2737 0.81 +0.2 3,076,763 + Pf1N1B4_2737 0.81 +0.2 3,076,764 - Pf1N1B4_2737 0.81 -0.6 3,076,764 - Pf1N1B4_2737 0.81 -1.3 3,076,862 + -0.8 3,076,863 - Pf1N1B4_2737 0.88 -1.3 3,077,013 + +0.2 3,077,013 + +2.1 3,077,013 + +0.6 3,077,013 + +0.2 3,077,013 + -1.5 3,077,014 - +0.2 3,077,086 + +0.2 3,077,086 + +0.2 3,077,087 - -1.1 3,077,087 - -1.8 3,077,173 + -1.1 3,077,174 - +0.2 3,077,174 - -0.8 3,077,252 - +0.4 3,077,487 + Pf1N1B4_2738 0.25 -0.3 3,077,487 + Pf1N1B4_2738 0.25 +0.2 3,077,487 + Pf1N1B4_2738 0.25 -0.5 3,077,488 - Pf1N1B4_2738 0.25 +0.7 3,077,488 - Pf1N1B4_2738 0.25 -0.8 3,077,509 - Pf1N1B4_2738 0.28 -0.5 3,077,523 + Pf1N1B4_2738 0.29 -1.8 3,077,523 + Pf1N1B4_2738 0.29 +0.1 3,077,523 + Pf1N1B4_2738 0.29 +0.8 3,077,524 - Pf1N1B4_2738 0.29 -1.3 3,077,524 - Pf1N1B4_2738 0.29 -0.8 3,077,524 - Pf1N1B4_2738 0.29 -0.4 3,077,553 + Pf1N1B4_2738 0.32 +0.2 3,077,553 + Pf1N1B4_2738 0.32 -1.2 3,077,554 - Pf1N1B4_2738 0.32 -2.1 3,077,751 + Pf1N1B4_2738 0.54 -1.3 3,077,751 + Pf1N1B4_2738 0.54 +1.5 3,077,805 + Pf1N1B4_2738 0.60 -0.2 3,077,805 + Pf1N1B4_2738 0.60 -1.3 3,077,805 + Pf1N1B4_2738 0.60 -0.8 3,077,806 - Pf1N1B4_2738 0.60 -0.8 3,077,806 - Pf1N1B4_2738 0.60 -1.8 3,077,976 + Pf1N1B4_2738 0.78 +0.5 3,077,976 + Pf1N1B4_2738 0.78 +2.2 3,077,976 + Pf1N1B4_2738 0.78 -1.2 3,077,976 + Pf1N1B4_2738 0.78 -0.4 3,077,976 + Pf1N1B4_2738 0.78 -1.8 3,077,977 - Pf1N1B4_2738 0.78 -0.2 3,077,977 - Pf1N1B4_2738 0.78 -1.1 3,077,977 - Pf1N1B4_2738 0.78 -3.3 3,077,977 - Pf1N1B4_2738 0.78 +0.8 3,077,987 - Pf1N1B4_2738 0.79 -0.6 3,077,988 + Pf1N1B4_2738 0.80 -0.9 3,077,988 + Pf1N1B4_2738 0.80 -0.3 3,077,988 + Pf1N1B4_2738 0.80 +0.8 3,077,988 + Pf1N1B4_2738 0.80 -1.6 3,077,988 + -1.3 3,077,988 + Pf1N1B4_2738 0.80 -1.3 3,077,988 + Pf1N1B4_2738 0.80 +0.2 3,077,988 + Pf1N1B4_2738 0.80 -1.3 3,077,988 + Pf1N1B4_2738 0.80 -1.1 3,077,988 + Pf1N1B4_2738 0.80 +1.2 3,077,988 + Pf1N1B4_2738 0.80 +1.2 3,077,988 + Pf1N1B4_2738 0.80 -0.8 3,077,988 + Pf1N1B4_2738 0.80 +0.2 3,077,988 + Pf1N1B4_2738 0.80 -0.3 3,077,989 - Pf1N1B4_2738 0.80 -0.4 3,077,989 - Pf1N1B4_2738 0.80 +0.2 3,078,144 + -1.3 3,078,145 - +1.2 3,078,145 - -0.8 3,078,156 + +0.2 3,078,156 + -0.2 3,078,157 - +0.2 3,078,157 - +0.1 3,078,157 - +0.8 3,078,184 + +0.7 3,078,184 + +0.0 3,078,184 + +0.8 3,078,184 + +0.2 3,078,185 - +1.8 3,078,185 - -1.8 3,078,185 - -0.8 3,078,186 + +0.6 3,078,186 + -0.3 3,078,186 + -2.1 3,078,200 + +0.8 3,078,200 + +0.2 3,078,200 + -1.2 3,078,220 + -0.0 3,078,220 + +0.2 3,078,221 - +0.2 3,078,231 + -2.1 3,078,231 + -0.6 3,078,231 + +0.2 3,078,231 + -0.8 3,078,231 + +1.0 3,078,232 - -1.8 3,078,232 - +1.6 3,078,232 - -0.3 3,078,232 - +0.2 3,078,538 + Pf1N1B4_2739 0.48 -0.8 3,078,539 - Pf1N1B4_2739 0.48 +2.6 3,078,593 + Pf1N1B4_2739 0.57 -0.4 3,078,791 + +0.2 3,078,792 - +1.7 3,078,792 - -2.2 3,078,833 + +0.2 3,078,851 + +0.7 3,078,851 + +0.4 3,078,851 + -0.8 3,078,851 + +0.2 3,078,852 - -1.3 3,078,852 - -1.0 3,078,852 - -1.3 3,078,852 - -1.1 3,078,852 - -3.2 3,078,879 + -1.3 3,079,057 - Pf1N1B4_2740 0.13 +0.6 3,079,127 + Pf1N1B4_2740 0.21 -0.0 3,079,128 - Pf1N1B4_2740 0.21 -1.1 3,079,128 - Pf1N1B4_2740 0.21 -1.1 3,079,166 + Pf1N1B4_2740 0.26 -0.2
Or see this region's nucleotide sequence