Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2722 and Pf1N1B4_2723 overlap by 6 nucleotides Pf1N1B4_2723 and Pf1N1B4_2724 are separated by 187 nucleotides Pf1N1B4_2724 and Pf1N1B4_2725 are separated by 435 nucleotides
Pf1N1B4_2722 - Ribosomal large subunit pseudouridine synthase E (EC 4.2.1.70), at 3,061,429 to 3,062,064
_2722
Pf1N1B4_2723 - FIG00954142: hypothetical protein, at 3,062,059 to 3,062,268
_2723
Pf1N1B4_2724 - Transcriptional regulator, AsnC family, at 3,062,456 to 3,062,935
_2724
Pf1N1B4_2725 - FIG00954854: hypothetical protein, at 3,063,371 to 3,064,366
_2725
Position (kb)
3062
3063 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3061.732 kb on + strand, within Pf1N1B4_2722 at 3061.732 kb on + strand, within Pf1N1B4_2722 at 3061.732 kb on + strand, within Pf1N1B4_2722 at 3061.732 kb on + strand, within Pf1N1B4_2722 at 3061.733 kb on - strand, within Pf1N1B4_2722 at 3061.733 kb on - strand, within Pf1N1B4_2722 at 3061.733 kb on - strand, within Pf1N1B4_2722 at 3061.733 kb on - strand, within Pf1N1B4_2722 at 3061.733 kb on - strand, within Pf1N1B4_2722 at 3061.733 kb on - strand, within Pf1N1B4_2722 at 3061.809 kb on + strand, within Pf1N1B4_2722 at 3062.060 kb on + strand at 3062.061 kb on - strand at 3062.062 kb on + strand at 3062.062 kb on + strand at 3062.062 kb on + strand at 3062.062 kb on + strand at 3062.062 kb on + strand at 3062.062 kb on + strand at 3062.063 kb on - strand at 3062.063 kb on - strand at 3062.131 kb on + strand, within Pf1N1B4_2723 at 3062.132 kb on - strand, within Pf1N1B4_2723 at 3062.294 kb on + strand at 3062.294 kb on + strand at 3062.295 kb on - strand at 3062.309 kb on + strand at 3062.309 kb on + strand at 3062.310 kb on - strand at 3062.310 kb on - strand at 3062.310 kb on - strand at 3062.311 kb on + strand at 3062.311 kb on + strand at 3062.312 kb on - strand at 3062.312 kb on - strand at 3062.361 kb on + strand at 3062.361 kb on + strand at 3062.361 kb on + strand at 3062.362 kb on - strand at 3062.362 kb on - strand at 3062.362 kb on - strand at 3062.506 kb on + strand, within Pf1N1B4_2724 at 3062.507 kb on - strand, within Pf1N1B4_2724 at 3062.507 kb on - strand, within Pf1N1B4_2724 at 3062.600 kb on + strand, within Pf1N1B4_2724 at 3062.600 kb on + strand, within Pf1N1B4_2724 at 3062.601 kb on - strand, within Pf1N1B4_2724 at 3062.601 kb on - strand, within Pf1N1B4_2724 at 3062.601 kb on - strand, within Pf1N1B4_2724 at 3062.613 kb on + strand, within Pf1N1B4_2724 at 3062.613 kb on + strand, within Pf1N1B4_2724 at 3062.614 kb on - strand, within Pf1N1B4_2724 at 3062.704 kb on + strand, within Pf1N1B4_2724 at 3062.704 kb on + strand, within Pf1N1B4_2724 at 3062.704 kb on + strand, within Pf1N1B4_2724 at 3062.705 kb on - strand, within Pf1N1B4_2724 at 3062.705 kb on - strand, within Pf1N1B4_2724 at 3062.730 kb on + strand, within Pf1N1B4_2724 at 3062.730 kb on + strand, within Pf1N1B4_2724 at 3062.730 kb on + strand, within Pf1N1B4_2724 at 3062.731 kb on - strand, within Pf1N1B4_2724 at 3062.731 kb on - strand, within Pf1N1B4_2724 at 3062.790 kb on + strand, within Pf1N1B4_2724 at 3062.790 kb on + strand, within Pf1N1B4_2724 at 3062.790 kb on + strand, within Pf1N1B4_2724 at 3062.790 kb on + strand, within Pf1N1B4_2724 at 3062.790 kb on + strand, within Pf1N1B4_2724 at 3062.790 kb on + strand, within Pf1N1B4_2724 at 3062.791 kb on - strand, within Pf1N1B4_2724 at 3062.791 kb on - strand, within Pf1N1B4_2724 at 3062.791 kb on - strand, within Pf1N1B4_2724 at 3062.791 kb on - strand, within Pf1N1B4_2724 at 3062.791 kb on - strand, within Pf1N1B4_2724 at 3062.891 kb on + strand at 3062.891 kb on + strand at 3062.891 kb on + strand at 3062.892 kb on - strand at 3062.892 kb on - strand at 3062.957 kb on + strand at 3062.971 kb on - strand at 3062.974 kb on + strand at 3063.048 kb on + strand at 3063.054 kb on + strand at 3063.054 kb on + strand at 3063.154 kb on + strand at 3063.155 kb on - strand at 3063.195 kb on + strand at 3063.195 kb on + strand at 3063.195 kb on + strand at 3063.195 kb on + strand at 3063.196 kb on - strand at 3063.196 kb on - strand at 3063.265 kb on - strand at 3063.280 kb on + strand at 3063.284 kb on + strand at 3063.284 kb on + strand at 3063.284 kb on + strand at 3063.284 kb on + strand at 3063.284 kb on + strand at 3063.284 kb on + strand at 3063.284 kb on + strand at 3063.285 kb on - strand at 3063.285 kb on - strand at 3063.337 kb on + strand at 3063.337 kb on + strand at 3063.337 kb on + strand at 3063.338 kb on - strand at 3063.338 kb on - strand at 3063.411 kb on + strand at 3063.411 kb on + strand at 3063.411 kb on + strand at 3063.411 kb on + strand at 3063.411 kb on + strand at 3063.411 kb on + strand at 3063.411 kb on + strand at 3063.412 kb on - strand at 3063.412 kb on - strand at 3063.412 kb on - strand at 3063.737 kb on + strand, within Pf1N1B4_2725 at 3063.761 kb on + strand, within Pf1N1B4_2725 at 3063.761 kb on + strand, within Pf1N1B4_2725 at 3063.761 kb on + strand, within Pf1N1B4_2725 at 3063.761 kb on + strand, within Pf1N1B4_2725 at 3063.761 kb on + strand, within Pf1N1B4_2725 at 3063.761 kb on + strand, within Pf1N1B4_2725 at 3063.761 kb on + strand, within Pf1N1B4_2725 at 3063.761 kb on + strand, within Pf1N1B4_2725 at 3063.761 kb on + strand, within Pf1N1B4_2725 at 3063.762 kb on - strand, within Pf1N1B4_2725 at 3063.762 kb on - strand, within Pf1N1B4_2725 at 3063.762 kb on - strand, within Pf1N1B4_2725 at 3063.762 kb on - strand, within Pf1N1B4_2725 at 3063.762 kb on - strand, within Pf1N1B4_2725 at 3063.869 kb on + strand, within Pf1N1B4_2725 at 3063.882 kb on - strand, within Pf1N1B4_2725 at 3063.911 kb on + strand, within Pf1N1B4_2725
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 3,061,732 + Pf1N1B4_2722 0.48 +0.3 3,061,732 + Pf1N1B4_2722 0.48 -0.7 3,061,732 + Pf1N1B4_2722 0.48 +0.1 3,061,732 + Pf1N1B4_2722 0.48 -0.5 3,061,733 - Pf1N1B4_2722 0.48 -0.7 3,061,733 - Pf1N1B4_2722 0.48 -0.5 3,061,733 - Pf1N1B4_2722 0.48 -0.5 3,061,733 - Pf1N1B4_2722 0.48 +0.9 3,061,733 - Pf1N1B4_2722 0.48 -0.0 3,061,733 - Pf1N1B4_2722 0.48 +0.3 3,061,809 + Pf1N1B4_2722 0.60 -0.5 3,062,060 + +1.3 3,062,061 - +0.9 3,062,062 + -2.8 3,062,062 + -0.5 3,062,062 + +0.6 3,062,062 + -0.3 3,062,062 + +0.8 3,062,062 + -0.5 3,062,063 - +2.3 3,062,063 - +0.7 3,062,131 + Pf1N1B4_2723 0.34 -0.3 3,062,132 - Pf1N1B4_2723 0.35 +0.5 3,062,294 + -0.9 3,062,294 + +1.3 3,062,295 - -1.7 3,062,309 + +0.6 3,062,309 + -2.0 3,062,310 - -0.7 3,062,310 - +0.3 3,062,310 - -0.5 3,062,311 + +0.6 3,062,311 + +0.9 3,062,312 - +1.3 3,062,312 - -0.7 3,062,361 + -0.3 3,062,361 + +0.3 3,062,361 + +1.3 3,062,362 - +0.3 3,062,362 - +0.3 3,062,362 - +0.3 3,062,506 + Pf1N1B4_2724 0.10 -1.0 3,062,507 - Pf1N1B4_2724 0.11 -3.7 3,062,507 - Pf1N1B4_2724 0.11 -2.5 3,062,600 + Pf1N1B4_2724 0.30 -2.9 3,062,600 + Pf1N1B4_2724 0.30 -0.7 3,062,601 - Pf1N1B4_2724 0.30 -2.3 3,062,601 - Pf1N1B4_2724 0.30 +0.3 3,062,601 - Pf1N1B4_2724 0.30 -1.3 3,062,613 + Pf1N1B4_2724 0.33 -2.3 3,062,613 + Pf1N1B4_2724 0.33 -2.9 3,062,614 - Pf1N1B4_2724 0.33 -0.1 3,062,704 + Pf1N1B4_2724 0.52 -0.9 3,062,704 + Pf1N1B4_2724 0.52 -1.3 3,062,704 + Pf1N1B4_2724 0.52 -2.7 3,062,705 - Pf1N1B4_2724 0.52 -1.7 3,062,705 - Pf1N1B4_2724 0.52 -0.3 3,062,730 + Pf1N1B4_2724 0.57 -0.3 3,062,730 + Pf1N1B4_2724 0.57 -2.9 3,062,730 + Pf1N1B4_2724 0.57 -2.3 3,062,731 - Pf1N1B4_2724 0.57 -2.3 3,062,731 - Pf1N1B4_2724 0.57 +0.9 3,062,790 + Pf1N1B4_2724 0.70 +0.3 3,062,790 + Pf1N1B4_2724 0.70 -2.9 3,062,790 + Pf1N1B4_2724 0.70 -1.9 3,062,790 + Pf1N1B4_2724 0.70 -0.7 3,062,790 + Pf1N1B4_2724 0.70 -2.0 3,062,790 + Pf1N1B4_2724 0.70 +0.3 3,062,791 - Pf1N1B4_2724 0.70 -3.6 3,062,791 - Pf1N1B4_2724 0.70 -2.5 3,062,791 - Pf1N1B4_2724 0.70 -0.7 3,062,791 - Pf1N1B4_2724 0.70 -2.0 3,062,791 - Pf1N1B4_2724 0.70 -2.3 3,062,891 + +1.1 3,062,891 + -2.3 3,062,891 + -3.0 3,062,892 - +0.3 3,062,892 - +0.3 3,062,957 + -1.2 3,062,971 - -0.7 3,062,974 + -0.8 3,063,048 + +0.8 3,063,054 + -2.5 3,063,054 + -3.0 3,063,154 + -1.3 3,063,155 - -0.5 3,063,195 + -2.0 3,063,195 + -1.0 3,063,195 + -2.3 3,063,195 + -1.9 3,063,196 - -0.7 3,063,196 - +1.0 3,063,265 - -0.6 3,063,280 + +1.3 3,063,284 + +0.3 3,063,284 + +0.3 3,063,284 + -0.3 3,063,284 + +0.3 3,063,284 + -0.7 3,063,284 + -0.2 3,063,284 + +0.3 3,063,285 - +1.3 3,063,285 - -2.0 3,063,337 + -0.3 3,063,337 + -1.3 3,063,337 + +0.3 3,063,338 - -0.7 3,063,338 - -1.3 3,063,411 + -1.3 3,063,411 + -0.7 3,063,411 + +0.3 3,063,411 + +0.6 3,063,411 + +0.3 3,063,411 + +0.0 3,063,411 + +0.9 3,063,412 - -0.7 3,063,412 - +0.3 3,063,412 - -1.0 3,063,737 + Pf1N1B4_2725 0.37 +0.3 3,063,761 + Pf1N1B4_2725 0.39 +0.0 3,063,761 + Pf1N1B4_2725 0.39 -0.7 3,063,761 + Pf1N1B4_2725 0.39 -0.0 3,063,761 + Pf1N1B4_2725 0.39 -0.7 3,063,761 + Pf1N1B4_2725 0.39 +0.3 3,063,761 + Pf1N1B4_2725 0.39 -0.7 3,063,761 + Pf1N1B4_2725 0.39 -0.3 3,063,761 + Pf1N1B4_2725 0.39 +0.2 3,063,761 + Pf1N1B4_2725 0.39 -1.3 3,063,762 - Pf1N1B4_2725 0.39 -1.0 3,063,762 - Pf1N1B4_2725 0.39 +1.6 3,063,762 - Pf1N1B4_2725 0.39 +0.6 3,063,762 - Pf1N1B4_2725 0.39 -1.3 3,063,762 - Pf1N1B4_2725 0.39 -0.3 3,063,869 + Pf1N1B4_2725 0.50 +0.9 3,063,882 - Pf1N1B4_2725 0.51 +0.3 3,063,911 + Pf1N1B4_2725 0.54 +1.4
Or see this region's nucleotide sequence