Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2656 and Pf1N1B4_2657 are separated by 279 nucleotides Pf1N1B4_2657 and Pf1N1B4_2658 overlap by 4 nucleotides Pf1N1B4_2658 and Pf1N1B4_2659 overlap by 1 nucleotides Pf1N1B4_2659 and Pf1N1B4_2660 are separated by 108 nucleotides
Pf1N1B4_2656 - hypothetical protein, at 2,992,241 to 2,992,963
_2656
Pf1N1B4_2657 - GTP cyclohydrolase II (EC 3.5.4.25), at 2,993,243 to 2,993,860
_2657
Pf1N1B4_2658 - Permeases of the major facilitator superfamily, at 2,993,857 to 2,994,276
_2658
Pf1N1B4_2659 - Vitamin B12 ABC transporter, B12-binding component BtuF, at 2,994,276 to 2,995,076
_2659
Pf1N1B4_2660 - Outer membrane vitamin B12 receptor BtuB, at 2,995,185 to 2,997,068
_2660
Position (kb)
2993
2994
2995 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2992.890 kb on + strand, within Pf1N1B4_2656 at 2992.890 kb on + strand, within Pf1N1B4_2656 at 2992.890 kb on + strand, within Pf1N1B4_2656 at 2992.891 kb on - strand at 2992.891 kb on - strand at 2992.891 kb on - strand at 2992.944 kb on - strand at 2992.944 kb on - strand at 2992.976 kb on + strand at 2992.976 kb on + strand at 2992.976 kb on + strand at 2993.016 kb on + strand at 2993.017 kb on - strand at 2993.017 kb on - strand at 2993.103 kb on + strand at 2993.113 kb on + strand at 2993.114 kb on - strand at 2993.168 kb on + strand at 2993.169 kb on - strand at 2993.178 kb on + strand at 2993.178 kb on + strand at 2993.192 kb on + strand at 2993.192 kb on + strand at 2993.192 kb on + strand at 2993.219 kb on + strand at 2993.219 kb on + strand at 2993.223 kb on + strand at 2993.223 kb on + strand at 2993.223 kb on + strand at 2993.223 kb on + strand at 2993.223 kb on + strand at 2993.223 kb on + strand at 2993.223 kb on + strand at 2993.223 kb on + strand at 2993.223 kb on + strand at 2993.223 kb on + strand at 2993.223 kb on + strand at 2993.225 kb on + strand at 2993.225 kb on + strand at 2993.225 kb on + strand at 2993.225 kb on + strand at 2993.225 kb on + strand at 2993.225 kb on + strand at 2993.225 kb on + strand at 2993.225 kb on + strand at 2993.225 kb on + strand at 2993.225 kb on + strand at 2993.225 kb on + strand at 2993.905 kb on + strand, within Pf1N1B4_2658 at 2993.906 kb on - strand, within Pf1N1B4_2658 at 2994.244 kb on + strand at 2994.244 kb on + strand at 2994.244 kb on + strand at 2994.244 kb on + strand at 2994.244 kb on + strand at 2994.244 kb on + strand at 2994.245 kb on - strand at 2994.245 kb on - strand at 2994.245 kb on - strand at 2994.245 kb on - strand at 2994.245 kb on - strand at 2994.256 kb on - strand at 2994.468 kb on + strand, within Pf1N1B4_2659 at 2994.469 kb on - strand, within Pf1N1B4_2659 at 2994.469 kb on - strand, within Pf1N1B4_2659 at 2994.469 kb on - strand, within Pf1N1B4_2659 at 2994.469 kb on - strand, within Pf1N1B4_2659 at 2994.469 kb on - strand, within Pf1N1B4_2659 at 2994.469 kb on - strand, within Pf1N1B4_2659 at 2994.469 kb on - strand, within Pf1N1B4_2659 at 2994.585 kb on + strand, within Pf1N1B4_2659 at 2994.585 kb on + strand, within Pf1N1B4_2659 at 2994.585 kb on + strand, within Pf1N1B4_2659 at 2994.585 kb on + strand, within Pf1N1B4_2659 at 2994.585 kb on + strand at 2994.585 kb on + strand, within Pf1N1B4_2659 at 2994.585 kb on + strand, within Pf1N1B4_2659 at 2994.585 kb on + strand, within Pf1N1B4_2659 at 2994.585 kb on + strand, within Pf1N1B4_2659 at 2994.585 kb on + strand, within Pf1N1B4_2659 at 2994.585 kb on + strand at 2994.585 kb on + strand, within Pf1N1B4_2659 at 2994.585 kb on + strand, within Pf1N1B4_2659 at 2994.585 kb on + strand, within Pf1N1B4_2659 at 2994.585 kb on + strand, within Pf1N1B4_2659 at 2994.585 kb on + strand, within Pf1N1B4_2659 at 2994.585 kb on + strand, within Pf1N1B4_2659 at 2994.586 kb on - strand, within Pf1N1B4_2659 at 2994.586 kb on - strand, within Pf1N1B4_2659 at 2994.586 kb on - strand, within Pf1N1B4_2659 at 2994.586 kb on - strand, within Pf1N1B4_2659 at 2994.586 kb on - strand, within Pf1N1B4_2659 at 2994.586 kb on - strand, within Pf1N1B4_2659 at 2994.586 kb on - strand, within Pf1N1B4_2659 at 2994.586 kb on - strand, within Pf1N1B4_2659 at 2994.586 kb on - strand at 2994.586 kb on - strand, within Pf1N1B4_2659 at 2994.586 kb on - strand, within Pf1N1B4_2659 at 2994.586 kb on - strand, within Pf1N1B4_2659 at 2994.586 kb on - strand, within Pf1N1B4_2659 at 2994.586 kb on - strand, within Pf1N1B4_2659 at 2994.586 kb on - strand, within Pf1N1B4_2659 at 2994.739 kb on + strand, within Pf1N1B4_2659 at 2994.740 kb on - strand, within Pf1N1B4_2659 at 2994.750 kb on + strand, within Pf1N1B4_2659 at 2994.751 kb on - strand, within Pf1N1B4_2659 at 2994.774 kb on + strand, within Pf1N1B4_2659 at 2994.774 kb on + strand, within Pf1N1B4_2659 at 2994.775 kb on - strand, within Pf1N1B4_2659 at 2995.267 kb on + strand at 2995.267 kb on + strand at 2995.268 kb on - strand at 2995.268 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 2,992,890 + Pf1N1B4_2656 0.90 +0.3 2,992,890 + Pf1N1B4_2656 0.90 +0.3 2,992,890 + Pf1N1B4_2656 0.90 -0.7 2,992,891 - -0.7 2,992,891 - -0.7 2,992,891 - +2.3 2,992,944 - +1.9 2,992,944 - -1.3 2,992,976 + +0.3 2,992,976 + -0.2 2,992,976 + -0.1 2,993,016 + -0.9 2,993,017 - +1.3 2,993,017 - +0.3 2,993,103 + +0.7 2,993,113 + -0.5 2,993,114 - +0.3 2,993,168 + +0.2 2,993,169 - +1.9 2,993,178 + -1.3 2,993,178 + -0.0 2,993,192 + -2.3 2,993,192 + +1.3 2,993,192 + +0.6 2,993,219 + +0.9 2,993,219 + -1.3 2,993,223 + +1.5 2,993,223 + +1.9 2,993,223 + +0.3 2,993,223 + +0.6 2,993,223 + +0.6 2,993,223 + +1.3 2,993,223 + -1.0 2,993,223 + +0.3 2,993,223 + +1.1 2,993,223 + -0.7 2,993,223 + -0.7 2,993,225 + +0.9 2,993,225 + -0.1 2,993,225 + -1.0 2,993,225 + -1.7 2,993,225 + +0.3 2,993,225 + -0.1 2,993,225 + +0.3 2,993,225 + -0.7 2,993,225 + +0.1 2,993,225 + +0.3 2,993,225 + +1.6 2,993,905 + Pf1N1B4_2658 0.11 +0.3 2,993,906 - Pf1N1B4_2658 0.12 +0.6 2,994,244 + +0.8 2,994,244 + +0.7 2,994,244 + -1.7 2,994,244 + +0.3 2,994,244 + -0.3 2,994,244 + +1.3 2,994,245 - -0.4 2,994,245 - -0.1 2,994,245 - -0.7 2,994,245 - +0.7 2,994,245 - -0.7 2,994,256 - -0.3 2,994,468 + Pf1N1B4_2659 0.24 +0.3 2,994,469 - Pf1N1B4_2659 0.24 -0.3 2,994,469 - Pf1N1B4_2659 0.24 -0.4 2,994,469 - Pf1N1B4_2659 0.24 -2.3 2,994,469 - Pf1N1B4_2659 0.24 +0.3 2,994,469 - Pf1N1B4_2659 0.24 +0.2 2,994,469 - Pf1N1B4_2659 0.24 -0.6 2,994,469 - Pf1N1B4_2659 0.24 -0.9 2,994,585 + Pf1N1B4_2659 0.39 -2.3 2,994,585 + Pf1N1B4_2659 0.39 +1.9 2,994,585 + Pf1N1B4_2659 0.39 -0.4 2,994,585 + Pf1N1B4_2659 0.39 -2.5 2,994,585 + +0.3 2,994,585 + Pf1N1B4_2659 0.39 +0.3 2,994,585 + Pf1N1B4_2659 0.39 -0.7 2,994,585 + Pf1N1B4_2659 0.39 -1.0 2,994,585 + Pf1N1B4_2659 0.39 -0.7 2,994,585 + Pf1N1B4_2659 0.39 +0.3 2,994,585 + +1.3 2,994,585 + Pf1N1B4_2659 0.39 +0.3 2,994,585 + Pf1N1B4_2659 0.39 -1.3 2,994,585 + Pf1N1B4_2659 0.39 -0.7 2,994,585 + Pf1N1B4_2659 0.39 +1.9 2,994,585 + Pf1N1B4_2659 0.39 -0.7 2,994,585 + Pf1N1B4_2659 0.39 -0.4 2,994,586 - Pf1N1B4_2659 0.39 -0.9 2,994,586 - Pf1N1B4_2659 0.39 -0.7 2,994,586 - Pf1N1B4_2659 0.39 -0.7 2,994,586 - Pf1N1B4_2659 0.39 -0.1 2,994,586 - Pf1N1B4_2659 0.39 -0.7 2,994,586 - Pf1N1B4_2659 0.39 +0.3 2,994,586 - Pf1N1B4_2659 0.39 -1.9 2,994,586 - Pf1N1B4_2659 0.39 -2.0 2,994,586 - -0.7 2,994,586 - Pf1N1B4_2659 0.39 -0.7 2,994,586 - Pf1N1B4_2659 0.39 +0.3 2,994,586 - Pf1N1B4_2659 0.39 +1.3 2,994,586 - Pf1N1B4_2659 0.39 +0.7 2,994,586 - Pf1N1B4_2659 0.39 +1.6 2,994,586 - Pf1N1B4_2659 0.39 -0.1 2,994,739 + Pf1N1B4_2659 0.58 -0.3 2,994,740 - Pf1N1B4_2659 0.58 -1.0 2,994,750 + Pf1N1B4_2659 0.59 -1.3 2,994,751 - Pf1N1B4_2659 0.59 -1.3 2,994,774 + Pf1N1B4_2659 0.62 -2.3 2,994,774 + Pf1N1B4_2659 0.62 +1.6 2,994,775 - Pf1N1B4_2659 0.62 +2.6 2,995,267 + -1.5 2,995,267 + -0.4 2,995,268 - -2.1 2,995,268 - -0.7
Or see this region's nucleotide sequence