Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_249 and Pf1N1B4_250 are separated by 181 nucleotides Pf1N1B4_250 and Pf1N1B4_251 are separated by 256 nucleotides Pf1N1B4_251 and Pf1N1B4_252 are separated by 8 nucleotides
Pf1N1B4_249 - Deoxyguanosinetriphosphate triphosphohydrolase (EC 3.1.5.1), at 305,561 to 306,892
_249
Pf1N1B4_250 - response regulator, at 307,074 to 307,466
_250
Pf1N1B4_251 - DNA-binding response regulator, LuxR family, at 307,723 to 308,349
_251
Pf1N1B4_252 - Hybrid sensory histidine kinase in two-component regulatory system with EvgA, at 308,358 to 311,261
_252
Position (kb) 307
308
309 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 306.745 kb on - strand, within Pf1N1B4_249 at 306.789 kb on + strand at 306.790 kb on - strand at 306.790 kb on - strand at 306.790 kb on - strand at 306.842 kb on + strand at 306.842 kb on + strand at 306.842 kb on + strand at 306.842 kb on + strand at 306.842 kb on + strand at 306.842 kb on + strand at 306.843 kb on - strand at 306.843 kb on - strand at 306.843 kb on - strand at 306.843 kb on - strand at 306.843 kb on - strand at 306.956 kb on + strand at 306.957 kb on - strand at 306.982 kb on - strand at 307.030 kb on + strand at 307.031 kb on - strand at 307.073 kb on + strand at 307.073 kb on + strand at 307.074 kb on - strand at 307.074 kb on - strand at 307.074 kb on - strand at 307.291 kb on - strand, within Pf1N1B4_250 at 307.325 kb on - strand, within Pf1N1B4_250 at 307.555 kb on - strand at 307.593 kb on + strand at 307.597 kb on + strand at 307.644 kb on + strand at 307.738 kb on - strand at 307.799 kb on - strand, within Pf1N1B4_251 at 308.021 kb on - strand, within Pf1N1B4_251 at 308.024 kb on + strand, within Pf1N1B4_251 at 308.024 kb on + strand at 308.025 kb on - strand at 308.025 kb on - strand, within Pf1N1B4_251 at 308.075 kb on + strand, within Pf1N1B4_251 at 308.076 kb on - strand at 308.089 kb on + strand, within Pf1N1B4_251 at 308.266 kb on + strand, within Pf1N1B4_251 at 308.386 kb on + strand at 308.451 kb on + strand at 308.452 kb on - strand at 308.549 kb on + strand at 308.565 kb on + strand at 308.601 kb on + strand at 308.601 kb on + strand at 308.601 kb on + strand at 308.785 kb on - strand, within Pf1N1B4_252 at 308.874 kb on + strand, within Pf1N1B4_252 at 308.874 kb on + strand, within Pf1N1B4_252 at 308.875 kb on - strand, within Pf1N1B4_252 at 308.875 kb on - strand, within Pf1N1B4_252 at 308.875 kb on - strand, within Pf1N1B4_252 at 308.875 kb on - strand, within Pf1N1B4_252 at 308.875 kb on - strand, within Pf1N1B4_252 at 308.875 kb on - strand, within Pf1N1B4_252 at 308.875 kb on - strand, within Pf1N1B4_252 at 308.880 kb on + strand, within Pf1N1B4_252 at 308.880 kb on + strand, within Pf1N1B4_252 at 308.880 kb on + strand, within Pf1N1B4_252 at 308.881 kb on - strand, within Pf1N1B4_252 at 308.881 kb on - strand, within Pf1N1B4_252 at 308.881 kb on - strand, within Pf1N1B4_252 at 308.934 kb on + strand, within Pf1N1B4_252 at 308.935 kb on - strand, within Pf1N1B4_252 at 308.944 kb on - strand, within Pf1N1B4_252 at 309.015 kb on + strand, within Pf1N1B4_252 at 309.015 kb on + strand, within Pf1N1B4_252 at 309.015 kb on + strand, within Pf1N1B4_252 at 309.016 kb on - strand, within Pf1N1B4_252 at 309.016 kb on - strand, within Pf1N1B4_252 at 309.016 kb on - strand, within Pf1N1B4_252 at 309.016 kb on - strand, within Pf1N1B4_252 at 309.016 kb on - strand, within Pf1N1B4_252 at 309.019 kb on + strand, within Pf1N1B4_252 at 309.019 kb on + strand, within Pf1N1B4_252 at 309.019 kb on + strand, within Pf1N1B4_252 at 309.020 kb on - strand, within Pf1N1B4_252 at 309.020 kb on - strand, within Pf1N1B4_252 at 309.020 kb on - strand, within Pf1N1B4_252 at 309.020 kb on - strand, within Pf1N1B4_252
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 306,745 - Pf1N1B4_249 0.89 -0.1 306,789 + +0.7 306,790 - -1.3 306,790 - -0.3 306,790 - +0.7 306,842 + -0.3 306,842 + -1.3 306,842 + +1.3 306,842 + -0.9 306,842 + -1.3 306,842 + -0.3 306,843 - +1.1 306,843 - +0.3 306,843 - -0.7 306,843 - +2.3 306,843 - +0.3 306,956 + +0.3 306,957 - +0.1 306,982 - +0.7 307,030 + -0.9 307,031 - -1.3 307,073 + -1.3 307,073 + +0.3 307,074 - -0.5 307,074 - -0.8 307,074 - -0.3 307,291 - Pf1N1B4_250 0.55 -0.3 307,325 - Pf1N1B4_250 0.64 +0.3 307,555 - -1.0 307,593 + -0.9 307,597 + -2.3 307,644 + +0.1 307,738 - -1.3 307,799 - Pf1N1B4_251 0.12 +0.5 308,021 - Pf1N1B4_251 0.48 -1.1 308,024 + Pf1N1B4_251 0.48 +0.7 308,024 + -1.3 308,025 - +0.7 308,025 - Pf1N1B4_251 0.48 +0.3 308,075 + Pf1N1B4_251 0.56 +1.3 308,076 - -1.9 308,089 + Pf1N1B4_251 0.58 +0.5 308,266 + Pf1N1B4_251 0.87 +0.3 308,386 + -0.1 308,451 + -1.6 308,452 - -0.3 308,549 + -3.1 308,565 + +0.4 308,601 + -0.3 308,601 + +0.1 308,601 + -0.8 308,785 - Pf1N1B4_252 0.15 -0.5 308,874 + Pf1N1B4_252 0.18 -0.6 308,874 + Pf1N1B4_252 0.18 +0.9 308,875 - Pf1N1B4_252 0.18 -1.0 308,875 - Pf1N1B4_252 0.18 -0.3 308,875 - Pf1N1B4_252 0.18 +0.3 308,875 - Pf1N1B4_252 0.18 -0.3 308,875 - Pf1N1B4_252 0.18 -1.3 308,875 - Pf1N1B4_252 0.18 +0.5 308,875 - Pf1N1B4_252 0.18 +0.3 308,880 + Pf1N1B4_252 0.18 +1.3 308,880 + Pf1N1B4_252 0.18 +0.6 308,880 + Pf1N1B4_252 0.18 -1.0 308,881 - Pf1N1B4_252 0.18 +0.9 308,881 - Pf1N1B4_252 0.18 -0.3 308,881 - Pf1N1B4_252 0.18 +0.7 308,934 + Pf1N1B4_252 0.20 -0.6 308,935 - Pf1N1B4_252 0.20 +1.3 308,944 - Pf1N1B4_252 0.20 +0.1 309,015 + Pf1N1B4_252 0.23 -1.9 309,015 + Pf1N1B4_252 0.23 -0.0 309,015 + Pf1N1B4_252 0.23 +0.1 309,016 - Pf1N1B4_252 0.23 +0.1 309,016 - Pf1N1B4_252 0.23 -2.3 309,016 - Pf1N1B4_252 0.23 +0.2 309,016 - Pf1N1B4_252 0.23 +0.1 309,016 - Pf1N1B4_252 0.23 +0.1 309,019 + Pf1N1B4_252 0.23 -1.3 309,019 + Pf1N1B4_252 0.23 -2.1 309,019 + Pf1N1B4_252 0.23 -2.3 309,020 - Pf1N1B4_252 0.23 -0.3 309,020 - Pf1N1B4_252 0.23 -1.3 309,020 - Pf1N1B4_252 0.23 +0.3 309,020 - Pf1N1B4_252 0.23 -0.3
Or see this region's nucleotide sequence