Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2491 and Pf1N1B4_2492 are separated by 120 nucleotides Pf1N1B4_2492 and Pf1N1B4_2493 are separated by 277 nucleotides Pf1N1B4_2493 and Pf1N1B4_2494 are separated by 228 nucleotides
Pf1N1B4_2491 - Dethiobiotin synthetase (EC 6.3.3.3), at 2,823,885 to 2,824,565
_2491
Pf1N1B4_2492 - FIG00954771: hypothetical protein, at 2,824,686 to 2,824,982
_2492
Pf1N1B4_2493 - Acyl-CoA dehydrogenase (EC 1.3.8.7), at 2,825,260 to 2,827,065
_2493
Pf1N1B4_2494 - GGDEF domain protein, at 2,827,294 to 2,828,583
_2494
Position (kb)
2825
2826
2827
2828 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2824.583 kb on + strand at 2824.583 kb on + strand at 2824.583 kb on + strand at 2824.730 kb on + strand, within Pf1N1B4_2492 at 2824.730 kb on + strand, within Pf1N1B4_2492 at 2824.880 kb on + strand, within Pf1N1B4_2492 at 2824.880 kb on + strand, within Pf1N1B4_2492 at 2824.881 kb on - strand, within Pf1N1B4_2492 at 2824.881 kb on - strand, within Pf1N1B4_2492 at 2824.881 kb on - strand, within Pf1N1B4_2492 at 2824.906 kb on + strand, within Pf1N1B4_2492 at 2824.906 kb on + strand, within Pf1N1B4_2492 at 2824.906 kb on + strand, within Pf1N1B4_2492 at 2824.906 kb on + strand, within Pf1N1B4_2492 at 2824.906 kb on + strand, within Pf1N1B4_2492 at 2824.907 kb on - strand, within Pf1N1B4_2492 at 2824.907 kb on - strand at 2824.970 kb on + strand at 2824.971 kb on - strand at 2824.971 kb on - strand at 2825.038 kb on + strand at 2825.038 kb on + strand at 2825.038 kb on + strand at 2825.039 kb on - strand at 2825.039 kb on - strand at 2825.039 kb on - strand at 2825.057 kb on + strand at 2825.057 kb on + strand at 2825.058 kb on - strand at 2825.058 kb on - strand at 2825.058 kb on - strand at 2825.058 kb on - strand at 2825.067 kb on + strand at 2825.067 kb on + strand at 2825.067 kb on + strand at 2825.067 kb on + strand at 2825.067 kb on + strand at 2825.068 kb on - strand at 2825.068 kb on - strand at 2825.068 kb on - strand at 2825.068 kb on - strand at 2825.068 kb on - strand at 2825.178 kb on + strand at 2825.178 kb on + strand at 2825.179 kb on - strand at 2825.179 kb on - strand at 2825.179 kb on - strand at 2825.237 kb on + strand at 2825.237 kb on + strand at 2825.260 kb on - strand at 2825.290 kb on - strand at 2825.506 kb on + strand, within Pf1N1B4_2493 at 2825.507 kb on - strand, within Pf1N1B4_2493 at 2825.552 kb on + strand, within Pf1N1B4_2493 at 2825.707 kb on + strand, within Pf1N1B4_2493 at 2825.824 kb on + strand, within Pf1N1B4_2493 at 2826.296 kb on + strand, within Pf1N1B4_2493 at 2826.497 kb on + strand, within Pf1N1B4_2493 at 2826.577 kb on + strand, within Pf1N1B4_2493 at 2826.621 kb on + strand, within Pf1N1B4_2493 at 2826.835 kb on + strand, within Pf1N1B4_2493 at 2826.859 kb on + strand, within Pf1N1B4_2493 at 2826.959 kb on - strand at 2826.970 kb on - strand at 2826.998 kb on + strand at 2826.998 kb on + strand at 2827.060 kb on + strand at 2827.060 kb on + strand at 2827.060 kb on + strand at 2827.061 kb on - strand at 2827.064 kb on - strand at 2827.171 kb on - strand at 2827.217 kb on - strand at 2827.217 kb on - strand at 2827.217 kb on - strand at 2827.217 kb on - strand at 2827.217 kb on - strand at 2827.217 kb on - strand at 2827.217 kb on - strand at 2827.237 kb on - strand at 2827.237 kb on - strand at 2827.237 kb on - strand at 2827.313 kb on + strand at 2827.313 kb on + strand at 2827.340 kb on + strand at 2827.341 kb on - strand at 2827.347 kb on - strand at 2827.347 kb on - strand at 2827.347 kb on - strand at 2827.347 kb on - strand at 2827.372 kb on + strand at 2827.372 kb on + strand at 2827.372 kb on + strand at 2827.373 kb on - strand at 2827.373 kb on - strand at 2827.373 kb on - strand at 2827.422 kb on + strand at 2827.422 kb on + strand at 2827.422 kb on + strand at 2827.422 kb on + strand at 2827.422 kb on + strand at 2827.423 kb on - strand, within Pf1N1B4_2494 at 2827.423 kb on - strand, within Pf1N1B4_2494 at 2827.423 kb on - strand, within Pf1N1B4_2494 at 2827.423 kb on - strand, within Pf1N1B4_2494 at 2827.423 kb on - strand, within Pf1N1B4_2494 at 2827.423 kb on - strand, within Pf1N1B4_2494 at 2827.501 kb on + strand, within Pf1N1B4_2494 at 2827.501 kb on + strand, within Pf1N1B4_2494 at 2827.501 kb on + strand, within Pf1N1B4_2494 at 2827.501 kb on + strand, within Pf1N1B4_2494 at 2827.501 kb on + strand, within Pf1N1B4_2494 at 2827.501 kb on + strand, within Pf1N1B4_2494 at 2827.501 kb on + strand, within Pf1N1B4_2494 at 2827.501 kb on + strand, within Pf1N1B4_2494 at 2827.501 kb on + strand, within Pf1N1B4_2494 at 2827.502 kb on - strand, within Pf1N1B4_2494 at 2827.502 kb on - strand, within Pf1N1B4_2494 at 2827.502 kb on - strand, within Pf1N1B4_2494 at 2827.502 kb on - strand, within Pf1N1B4_2494 at 2827.502 kb on - strand, within Pf1N1B4_2494 at 2827.502 kb on - strand, within Pf1N1B4_2494 at 2827.502 kb on - strand, within Pf1N1B4_2494 at 2827.511 kb on - strand, within Pf1N1B4_2494 at 2827.537 kb on + strand, within Pf1N1B4_2494 at 2827.538 kb on - strand, within Pf1N1B4_2494 at 2827.538 kb on - strand, within Pf1N1B4_2494 at 2827.538 kb on - strand, within Pf1N1B4_2494 at 2827.541 kb on - strand, within Pf1N1B4_2494 at 2827.541 kb on - strand at 2827.610 kb on + strand, within Pf1N1B4_2494 at 2827.610 kb on + strand, within Pf1N1B4_2494 at 2827.611 kb on - strand, within Pf1N1B4_2494 at 2827.611 kb on - strand, within Pf1N1B4_2494 at 2828.008 kb on + strand, within Pf1N1B4_2494 at 2828.009 kb on - strand, within Pf1N1B4_2494
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 2,824,583 + -1.1 2,824,583 + +1.3 2,824,583 + +0.1 2,824,730 + Pf1N1B4_2492 0.15 +0.3 2,824,730 + Pf1N1B4_2492 0.15 +1.3 2,824,880 + Pf1N1B4_2492 0.65 +1.3 2,824,880 + Pf1N1B4_2492 0.65 +0.7 2,824,881 - Pf1N1B4_2492 0.66 +0.0 2,824,881 - Pf1N1B4_2492 0.66 -0.3 2,824,881 - Pf1N1B4_2492 0.66 +1.3 2,824,906 + Pf1N1B4_2492 0.74 -1.1 2,824,906 + Pf1N1B4_2492 0.74 -0.7 2,824,906 + Pf1N1B4_2492 0.74 +0.1 2,824,906 + Pf1N1B4_2492 0.74 -1.0 2,824,906 + Pf1N1B4_2492 0.74 -1.5 2,824,907 - Pf1N1B4_2492 0.74 +0.3 2,824,907 - +0.3 2,824,970 + -0.5 2,824,971 - +0.7 2,824,971 - -0.7 2,825,038 + -1.3 2,825,038 + -1.1 2,825,038 + +0.2 2,825,039 - -0.3 2,825,039 - -0.6 2,825,039 - -0.7 2,825,057 + -0.2 2,825,057 + -1.3 2,825,058 - +0.3 2,825,058 - -1.3 2,825,058 - +1.3 2,825,058 - +0.3 2,825,067 + -0.7 2,825,067 + -1.7 2,825,067 + -0.7 2,825,067 + -0.7 2,825,067 + +1.9 2,825,068 - -1.7 2,825,068 - -1.7 2,825,068 - -1.7 2,825,068 - +0.2 2,825,068 - +0.7 2,825,178 + +0.1 2,825,178 + +0.0 2,825,179 - +0.3 2,825,179 - +0.3 2,825,179 - -2.8 2,825,237 + -1.3 2,825,237 + -1.3 2,825,260 - -0.7 2,825,290 - +0.7 2,825,506 + Pf1N1B4_2493 0.14 -0.7 2,825,507 - Pf1N1B4_2493 0.14 +0.3 2,825,552 + Pf1N1B4_2493 0.16 -2.0 2,825,707 + Pf1N1B4_2493 0.25 -0.7 2,825,824 + Pf1N1B4_2493 0.31 -1.7 2,826,296 + Pf1N1B4_2493 0.57 +0.3 2,826,497 + Pf1N1B4_2493 0.68 +0.3 2,826,577 + Pf1N1B4_2493 0.73 -2.3 2,826,621 + Pf1N1B4_2493 0.75 -2.4 2,826,835 + Pf1N1B4_2493 0.87 +0.3 2,826,859 + Pf1N1B4_2493 0.89 -1.7 2,826,959 - -0.7 2,826,970 - +0.3 2,826,998 + -1.7 2,826,998 + -1.7 2,827,060 + -0.1 2,827,060 + -1.7 2,827,060 + -1.3 2,827,061 - -0.1 2,827,064 - +1.9 2,827,171 - -0.3 2,827,217 - -0.5 2,827,217 - -0.1 2,827,217 - -0.3 2,827,217 - +1.1 2,827,217 - -0.3 2,827,217 - -2.0 2,827,217 - -1.3 2,827,237 - -1.0 2,827,237 - -0.4 2,827,237 - +0.3 2,827,313 + +0.1 2,827,313 + -2.3 2,827,340 + +0.3 2,827,341 - -1.1 2,827,347 - +0.3 2,827,347 - +0.3 2,827,347 - -0.2 2,827,347 - +0.0 2,827,372 + +0.0 2,827,372 + +0.3 2,827,372 + +0.3 2,827,373 - -0.5 2,827,373 - +0.3 2,827,373 - -0.3 2,827,422 + +0.3 2,827,422 + +1.7 2,827,422 + +0.6 2,827,422 + -1.0 2,827,422 + -0.7 2,827,423 - Pf1N1B4_2494 0.10 +1.7 2,827,423 - Pf1N1B4_2494 0.10 -0.7 2,827,423 - Pf1N1B4_2494 0.10 -0.2 2,827,423 - Pf1N1B4_2494 0.10 +0.3 2,827,423 - Pf1N1B4_2494 0.10 -1.3 2,827,423 - Pf1N1B4_2494 0.10 +1.3 2,827,501 + Pf1N1B4_2494 0.16 +0.3 2,827,501 + Pf1N1B4_2494 0.16 -0.7 2,827,501 + Pf1N1B4_2494 0.16 +0.3 2,827,501 + Pf1N1B4_2494 0.16 +0.7 2,827,501 + Pf1N1B4_2494 0.16 +1.1 2,827,501 + Pf1N1B4_2494 0.16 -0.3 2,827,501 + Pf1N1B4_2494 0.16 -0.9 2,827,501 + Pf1N1B4_2494 0.16 -1.3 2,827,501 + Pf1N1B4_2494 0.16 +0.9 2,827,502 - Pf1N1B4_2494 0.16 +1.3 2,827,502 - Pf1N1B4_2494 0.16 -0.7 2,827,502 - Pf1N1B4_2494 0.16 +1.7 2,827,502 - Pf1N1B4_2494 0.16 +1.3 2,827,502 - Pf1N1B4_2494 0.16 +0.0 2,827,502 - Pf1N1B4_2494 0.16 +0.3 2,827,502 - Pf1N1B4_2494 0.16 +0.9 2,827,511 - Pf1N1B4_2494 0.17 +0.3 2,827,537 + Pf1N1B4_2494 0.19 +0.3 2,827,538 - Pf1N1B4_2494 0.19 -1.1 2,827,538 - Pf1N1B4_2494 0.19 -2.7 2,827,538 - Pf1N1B4_2494 0.19 -0.4 2,827,541 - Pf1N1B4_2494 0.19 +0.0 2,827,541 - -0.7 2,827,610 + Pf1N1B4_2494 0.24 -0.3 2,827,610 + Pf1N1B4_2494 0.24 -0.7 2,827,611 - Pf1N1B4_2494 0.25 +0.1 2,827,611 - Pf1N1B4_2494 0.25 +0.3 2,828,008 + Pf1N1B4_2494 0.55 +0.6 2,828,009 - Pf1N1B4_2494 0.55 +0.3
Or see this region's nucleotide sequence