Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2488 and Pf1N1B4_2489 overlap by 8 nucleotides Pf1N1B4_2489 and Pf1N1B4_2490 overlap by 8 nucleotides Pf1N1B4_2490 and Pf1N1B4_2491 overlap by 4 nucleotides Pf1N1B4_2491 and Pf1N1B4_2492 are separated by 120 nucleotides
Pf1N1B4_2488 - 8-amino-7-oxononanoate synthase (EC 2.3.1.47), at 2,821,181 to 2,822,359
_2488
Pf1N1B4_2489 - Biotin synthesis protein BioH, at 2,822,352 to 2,823,083
_2489
Pf1N1B4_2490 - Biotin synthesis protein BioC, at 2,823,076 to 2,823,888
_2490
Pf1N1B4_2491 - Dethiobiotin synthetase (EC 6.3.3.3), at 2,823,885 to 2,824,565
_2491
Pf1N1B4_2492 - FIG00954771: hypothetical protein, at 2,824,686 to 2,824,982
_2492
Position (kb)
2823
2824 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2822.298 kb on + strand at 2822.298 kb on + strand at 2822.299 kb on - strand at 2822.299 kb on - strand at 2822.305 kb on - strand at 2822.305 kb on - strand at 2822.305 kb on - strand at 2822.305 kb on - strand at 2822.305 kb on - strand at 2822.305 kb on - strand at 2822.813 kb on + strand, within Pf1N1B4_2489 at 2822.813 kb on + strand, within Pf1N1B4_2489 at 2822.813 kb on + strand at 2822.813 kb on + strand, within Pf1N1B4_2489 at 2822.813 kb on + strand, within Pf1N1B4_2489 at 2822.813 kb on + strand, within Pf1N1B4_2489 at 2822.813 kb on + strand, within Pf1N1B4_2489 at 2822.813 kb on + strand, within Pf1N1B4_2489 at 2822.813 kb on + strand, within Pf1N1B4_2489 at 2822.813 kb on + strand, within Pf1N1B4_2489 at 2822.813 kb on + strand, within Pf1N1B4_2489 at 2822.814 kb on - strand, within Pf1N1B4_2489 at 2823.070 kb on + strand at 2823.070 kb on + strand at 2823.071 kb on - strand at 2823.071 kb on - strand at 2823.086 kb on + strand at 2823.086 kb on + strand at 2823.087 kb on - strand at 2823.163 kb on + strand, within Pf1N1B4_2490 at 2823.163 kb on + strand, within Pf1N1B4_2490 at 2823.163 kb on + strand, within Pf1N1B4_2490 at 2823.163 kb on + strand, within Pf1N1B4_2490 at 2823.164 kb on - strand, within Pf1N1B4_2490 at 2823.209 kb on + strand, within Pf1N1B4_2490 at 2823.209 kb on + strand, within Pf1N1B4_2490 at 2823.209 kb on + strand, within Pf1N1B4_2490 at 2823.210 kb on - strand, within Pf1N1B4_2490 at 2823.210 kb on - strand, within Pf1N1B4_2490 at 2823.210 kb on - strand, within Pf1N1B4_2490 at 2823.531 kb on + strand, within Pf1N1B4_2490 at 2823.532 kb on - strand, within Pf1N1B4_2490 at 2823.542 kb on + strand, within Pf1N1B4_2490 at 2823.543 kb on - strand, within Pf1N1B4_2490 at 2823.543 kb on - strand, within Pf1N1B4_2490 at 2823.543 kb on - strand, within Pf1N1B4_2490 at 2823.543 kb on - strand, within Pf1N1B4_2490 at 2823.553 kb on + strand, within Pf1N1B4_2490 at 2823.554 kb on - strand, within Pf1N1B4_2490 at 2823.554 kb on - strand, within Pf1N1B4_2490 at 2823.554 kb on - strand, within Pf1N1B4_2490 at 2823.625 kb on + strand, within Pf1N1B4_2490 at 2823.626 kb on - strand, within Pf1N1B4_2490 at 2823.626 kb on - strand, within Pf1N1B4_2490 at 2823.694 kb on + strand, within Pf1N1B4_2490 at 2823.811 kb on + strand at 2823.811 kb on + strand at 2823.812 kb on - strand at 2823.812 kb on - strand at 2823.812 kb on - strand at 2823.850 kb on + strand at 2823.850 kb on + strand at 2823.850 kb on + strand at 2823.851 kb on - strand at 2823.884 kb on + strand at 2823.885 kb on - strand at 2823.885 kb on - strand at 2823.897 kb on + strand at 2823.897 kb on + strand at 2823.897 kb on + strand at 2823.897 kb on + strand at 2823.897 kb on + strand at 2823.898 kb on - strand at 2823.898 kb on - strand at 2823.898 kb on - strand at 2824.089 kb on + strand, within Pf1N1B4_2491 at 2824.089 kb on + strand, within Pf1N1B4_2491 at 2824.089 kb on + strand, within Pf1N1B4_2491 at 2824.089 kb on + strand, within Pf1N1B4_2491 at 2824.090 kb on - strand, within Pf1N1B4_2491 at 2824.090 kb on - strand, within Pf1N1B4_2491 at 2824.090 kb on - strand, within Pf1N1B4_2491 at 2824.090 kb on - strand, within Pf1N1B4_2491 at 2824.090 kb on - strand, within Pf1N1B4_2491 at 2824.583 kb on + strand at 2824.583 kb on + strand at 2824.583 kb on + strand at 2824.730 kb on + strand, within Pf1N1B4_2492 at 2824.730 kb on + strand, within Pf1N1B4_2492 at 2824.880 kb on + strand, within Pf1N1B4_2492 at 2824.880 kb on + strand, within Pf1N1B4_2492 at 2824.881 kb on - strand, within Pf1N1B4_2492 at 2824.881 kb on - strand, within Pf1N1B4_2492 at 2824.881 kb on - strand, within Pf1N1B4_2492
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 2,822,298 + -1.3 2,822,298 + -1.3 2,822,299 - -2.7 2,822,299 - -1.7 2,822,305 - -2.0 2,822,305 - -2.0 2,822,305 - -1.7 2,822,305 - -2.5 2,822,305 - -1.7 2,822,305 - -3.0 2,822,813 + Pf1N1B4_2489 0.63 -1.3 2,822,813 + Pf1N1B4_2489 0.63 -2.0 2,822,813 + +0.9 2,822,813 + Pf1N1B4_2489 0.63 -2.5 2,822,813 + Pf1N1B4_2489 0.63 -0.3 2,822,813 + Pf1N1B4_2489 0.63 -1.7 2,822,813 + Pf1N1B4_2489 0.63 -1.7 2,822,813 + Pf1N1B4_2489 0.63 -1.7 2,822,813 + Pf1N1B4_2489 0.63 +1.3 2,822,813 + Pf1N1B4_2489 0.63 +0.3 2,822,813 + Pf1N1B4_2489 0.63 -1.6 2,822,814 - Pf1N1B4_2489 0.63 +0.3 2,823,070 + +0.3 2,823,070 + +1.2 2,823,071 - -1.5 2,823,071 - -1.3 2,823,086 + +0.7 2,823,086 + -1.0 2,823,087 - -0.7 2,823,163 + Pf1N1B4_2490 0.11 -1.3 2,823,163 + Pf1N1B4_2490 0.11 +0.3 2,823,163 + Pf1N1B4_2490 0.11 -1.7 2,823,163 + Pf1N1B4_2490 0.11 -1.7 2,823,164 - Pf1N1B4_2490 0.11 -0.3 2,823,209 + Pf1N1B4_2490 0.16 -0.3 2,823,209 + Pf1N1B4_2490 0.16 -1.7 2,823,209 + Pf1N1B4_2490 0.16 -2.0 2,823,210 - Pf1N1B4_2490 0.16 +0.9 2,823,210 - Pf1N1B4_2490 0.16 +0.3 2,823,210 - Pf1N1B4_2490 0.16 -2.7 2,823,531 + Pf1N1B4_2490 0.56 -1.7 2,823,532 - Pf1N1B4_2490 0.56 -1.6 2,823,542 + Pf1N1B4_2490 0.57 -0.4 2,823,543 - Pf1N1B4_2490 0.57 -1.7 2,823,543 - Pf1N1B4_2490 0.57 -2.7 2,823,543 - Pf1N1B4_2490 0.57 -1.0 2,823,543 - Pf1N1B4_2490 0.57 -1.0 2,823,553 + Pf1N1B4_2490 0.59 -0.3 2,823,554 - Pf1N1B4_2490 0.59 +0.3 2,823,554 - Pf1N1B4_2490 0.59 -0.3 2,823,554 - Pf1N1B4_2490 0.59 -1.7 2,823,625 + Pf1N1B4_2490 0.68 -0.4 2,823,626 - Pf1N1B4_2490 0.68 -1.7 2,823,626 - Pf1N1B4_2490 0.68 +2.7 2,823,694 + Pf1N1B4_2490 0.76 -0.7 2,823,811 + -1.7 2,823,811 + +0.3 2,823,812 - -1.7 2,823,812 - -2.7 2,823,812 - -1.7 2,823,850 + -0.4 2,823,850 + +0.3 2,823,850 + -1.1 2,823,851 - -1.7 2,823,884 + -0.9 2,823,885 - -1.8 2,823,885 - +1.9 2,823,897 + -1.0 2,823,897 + +1.3 2,823,897 + -0.1 2,823,897 + -2.0 2,823,897 + +0.3 2,823,898 - -0.7 2,823,898 - -1.3 2,823,898 - -1.3 2,824,089 + Pf1N1B4_2491 0.30 -2.8 2,824,089 + Pf1N1B4_2491 0.30 -2.0 2,824,089 + Pf1N1B4_2491 0.30 -2.3 2,824,089 + Pf1N1B4_2491 0.30 -1.3 2,824,090 - Pf1N1B4_2491 0.30 -0.7 2,824,090 - Pf1N1B4_2491 0.30 -2.0 2,824,090 - Pf1N1B4_2491 0.30 -0.1 2,824,090 - Pf1N1B4_2491 0.30 -1.7 2,824,090 - Pf1N1B4_2491 0.30 -2.8 2,824,583 + -1.1 2,824,583 + +1.3 2,824,583 + +0.1 2,824,730 + Pf1N1B4_2492 0.15 +0.3 2,824,730 + Pf1N1B4_2492 0.15 +1.3 2,824,880 + Pf1N1B4_2492 0.65 +1.3 2,824,880 + Pf1N1B4_2492 0.65 +0.7 2,824,881 - Pf1N1B4_2492 0.66 +0.0 2,824,881 - Pf1N1B4_2492 0.66 -0.3 2,824,881 - Pf1N1B4_2492 0.66 +1.3
Or see this region's nucleotide sequence