Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2476 and Pf1N1B4_2477 are separated by 266 nucleotides Pf1N1B4_2477 and Pf1N1B4_2478 are separated by 173 nucleotides Pf1N1B4_2478 and Pf1N1B4_2479 are separated by 609 nucleotides
Pf1N1B4_2476 - Predicted signal-transduction protein containing cAMP-binding and CBS domains, at 2,807,554 to 2,809,494
_2476
Pf1N1B4_2477 - response regulator, at 2,809,761 to 2,810,213
_2477
Pf1N1B4_2478 - Malate synthase G (EC 2.3.3.9), at 2,810,387 to 2,812,564
_2478
Pf1N1B4_2479 - Glycine cleavage system transcriptional antiactivator GcvR, at 2,813,174 to 2,813,692
_2479
Position (kb)
2810
2811
2812
2813 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2809.411 kb on + strand at 2809.412 kb on - strand at 2809.412 kb on - strand at 2809.412 kb on - strand at 2809.489 kb on - strand at 2809.587 kb on + strand at 2809.587 kb on + strand at 2809.587 kb on + strand at 2809.587 kb on + strand at 2809.587 kb on + strand at 2809.587 kb on + strand at 2809.587 kb on + strand at 2809.587 kb on + strand at 2809.588 kb on - strand at 2809.588 kb on - strand at 2809.588 kb on - strand at 2809.588 kb on - strand at 2809.588 kb on - strand at 2809.593 kb on + strand at 2809.593 kb on + strand at 2809.593 kb on + strand at 2809.594 kb on - strand at 2809.594 kb on - strand at 2809.594 kb on - strand at 2809.594 kb on - strand at 2809.594 kb on - strand at 2809.594 kb on - strand at 2809.594 kb on - strand at 2809.594 kb on - strand at 2809.594 kb on - strand at 2809.597 kb on + strand at 2809.598 kb on - strand at 2809.598 kb on - strand at 2809.598 kb on - strand at 2809.619 kb on - strand at 2809.656 kb on + strand at 2809.656 kb on + strand at 2809.657 kb on - strand at 2809.657 kb on - strand at 2809.775 kb on + strand at 2809.775 kb on + strand at 2809.776 kb on - strand at 2809.954 kb on + strand, within Pf1N1B4_2477 at 2809.954 kb on + strand, within Pf1N1B4_2477 at 2809.954 kb on + strand, within Pf1N1B4_2477 at 2809.954 kb on + strand, within Pf1N1B4_2477 at 2810.128 kb on + strand, within Pf1N1B4_2477 at 2810.128 kb on + strand, within Pf1N1B4_2477 at 2810.128 kb on + strand, within Pf1N1B4_2477 at 2810.129 kb on - strand, within Pf1N1B4_2477 at 2810.385 kb on - strand at 2810.657 kb on - strand, within Pf1N1B4_2478 at 2810.657 kb on - strand at 2810.657 kb on - strand, within Pf1N1B4_2478 at 2810.749 kb on - strand, within Pf1N1B4_2478 at 2810.907 kb on + strand, within Pf1N1B4_2478 at 2810.972 kb on - strand, within Pf1N1B4_2478 at 2811.296 kb on - strand at 2811.388 kb on + strand, within Pf1N1B4_2478 at 2811.388 kb on + strand, within Pf1N1B4_2478 at 2811.391 kb on - strand, within Pf1N1B4_2478 at 2811.391 kb on - strand, within Pf1N1B4_2478 at 2811.391 kb on - strand, within Pf1N1B4_2478 at 2811.391 kb on - strand, within Pf1N1B4_2478 at 2811.439 kb on + strand, within Pf1N1B4_2478 at 2811.440 kb on - strand, within Pf1N1B4_2478 at 2811.479 kb on - strand, within Pf1N1B4_2478 at 2811.489 kb on - strand, within Pf1N1B4_2478 at 2811.506 kb on - strand, within Pf1N1B4_2478 at 2811.601 kb on + strand, within Pf1N1B4_2478 at 2811.603 kb on + strand, within Pf1N1B4_2478 at 2811.603 kb on + strand, within Pf1N1B4_2478 at 2811.603 kb on + strand, within Pf1N1B4_2478 at 2811.603 kb on + strand, within Pf1N1B4_2478 at 2811.603 kb on + strand, within Pf1N1B4_2478 at 2811.603 kb on + strand, within Pf1N1B4_2478 at 2811.603 kb on + strand, within Pf1N1B4_2478 at 2811.604 kb on - strand, within Pf1N1B4_2478 at 2811.604 kb on - strand, within Pf1N1B4_2478 at 2811.604 kb on - strand, within Pf1N1B4_2478 at 2811.604 kb on - strand, within Pf1N1B4_2478 at 2811.604 kb on - strand, within Pf1N1B4_2478 at 2811.604 kb on - strand, within Pf1N1B4_2478 at 2811.604 kb on - strand, within Pf1N1B4_2478 at 2811.604 kb on - strand, within Pf1N1B4_2478 at 2811.699 kb on + strand, within Pf1N1B4_2478 at 2811.700 kb on - strand, within Pf1N1B4_2478 at 2811.987 kb on + strand, within Pf1N1B4_2478 at 2811.987 kb on + strand, within Pf1N1B4_2478 at 2811.988 kb on - strand, within Pf1N1B4_2478 at 2811.988 kb on - strand, within Pf1N1B4_2478 at 2812.086 kb on + strand, within Pf1N1B4_2478 at 2812.087 kb on - strand, within Pf1N1B4_2478 at 2812.137 kb on + strand, within Pf1N1B4_2478 at 2812.137 kb on + strand, within Pf1N1B4_2478 at 2812.138 kb on - strand, within Pf1N1B4_2478 at 2812.149 kb on + strand, within Pf1N1B4_2478 at 2812.149 kb on + strand, within Pf1N1B4_2478 at 2812.323 kb on + strand, within Pf1N1B4_2478 at 2812.323 kb on + strand, within Pf1N1B4_2478 at 2812.323 kb on + strand, within Pf1N1B4_2478 at 2812.375 kb on + strand at 2812.376 kb on - strand at 2812.407 kb on - strand at 2812.635 kb on + strand at 2812.635 kb on + strand at 2812.636 kb on - strand at 2812.636 kb on - strand at 2812.652 kb on + strand at 2812.653 kb on - strand at 2812.653 kb on - strand at 2812.653 kb on - strand at 2812.658 kb on + strand at 2812.658 kb on + strand at 2812.658 kb on + strand at 2812.659 kb on - strand at 2812.659 kb on - strand at 2812.659 kb on - strand at 2812.767 kb on + strand at 2812.767 kb on + strand at 2812.768 kb on - strand at 2812.768 kb on - strand at 2812.771 kb on + strand at 2812.771 kb on + strand at 2812.772 kb on - strand at 2812.788 kb on + strand at 2812.789 kb on - strand at 2812.791 kb on + strand at 2812.792 kb on - strand at 2812.792 kb on - strand at 2812.823 kb on + strand at 2812.823 kb on + strand at 2812.823 kb on + strand at 2812.824 kb on - strand at 2812.824 kb on - strand at 2812.827 kb on + strand at 2812.827 kb on + strand at 2812.827 kb on + strand at 2812.828 kb on - strand at 2812.828 kb on - strand at 2812.925 kb on + strand at 2812.925 kb on + strand at 2812.926 kb on - strand at 2812.931 kb on + strand at 2812.931 kb on + strand at 2812.931 kb on + strand at 2812.931 kb on + strand at 2812.932 kb on - strand at 2813.017 kb on - strand at 2813.128 kb on + strand at 2813.128 kb on + strand at 2813.128 kb on + strand at 2813.128 kb on + strand at 2813.129 kb on - strand at 2813.129 kb on - strand at 2813.129 kb on - strand at 2813.129 kb on - strand at 2813.129 kb on - strand at 2813.129 kb on - strand at 2813.129 kb on - strand at 2813.129 kb on - strand at 2813.130 kb on + strand at 2813.130 kb on + strand at 2813.130 kb on + strand at 2813.130 kb on + strand at 2813.130 kb on + strand at 2813.130 kb on + strand at 2813.130 kb on + strand at 2813.130 kb on + strand at 2813.130 kb on + strand at 2813.131 kb on - strand at 2813.131 kb on - strand at 2813.131 kb on - strand at 2813.131 kb on - strand at 2813.131 kb on - strand at 2813.131 kb on - strand at 2813.131 kb on - strand at 2813.131 kb on - strand at 2813.143 kb on - strand at 2813.143 kb on - strand at 2813.188 kb on - strand at 2813.339 kb on + strand, within Pf1N1B4_2479 at 2813.352 kb on + strand, within Pf1N1B4_2479 at 2813.353 kb on - strand, within Pf1N1B4_2479 at 2813.541 kb on + strand, within Pf1N1B4_2479 at 2813.541 kb on + strand, within Pf1N1B4_2479 at 2813.542 kb on - strand, within Pf1N1B4_2479
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 2,809,411 + -1.0 2,809,412 - -1.0 2,809,412 - -1.2 2,809,412 - +0.3 2,809,489 - -0.1 2,809,587 + -0.1 2,809,587 + +0.9 2,809,587 + -0.2 2,809,587 + +1.6 2,809,587 + -1.5 2,809,587 + +1.3 2,809,587 + +0.3 2,809,587 + -0.2 2,809,588 - -1.2 2,809,588 - +1.3 2,809,588 - -0.5 2,809,588 - -0.7 2,809,588 - -0.9 2,809,593 + -0.4 2,809,593 + +0.7 2,809,593 + +1.9 2,809,594 - -0.2 2,809,594 - +0.5 2,809,594 - +1.3 2,809,594 - +0.8 2,809,594 - -0.1 2,809,594 - -1.7 2,809,594 - +0.1 2,809,594 - -0.1 2,809,594 - +2.3 2,809,597 + +0.5 2,809,598 - +1.0 2,809,598 - +0.9 2,809,598 - -0.1 2,809,619 - -0.3 2,809,656 + +0.7 2,809,656 + -1.2 2,809,657 - -1.7 2,809,657 - -0.2 2,809,775 + +0.7 2,809,775 + -2.0 2,809,776 - +1.1 2,809,954 + Pf1N1B4_2477 0.43 -0.1 2,809,954 + Pf1N1B4_2477 0.43 +0.0 2,809,954 + Pf1N1B4_2477 0.43 -0.4 2,809,954 + Pf1N1B4_2477 0.43 +0.3 2,810,128 + Pf1N1B4_2477 0.81 -0.2 2,810,128 + Pf1N1B4_2477 0.81 +0.9 2,810,128 + Pf1N1B4_2477 0.81 -0.9 2,810,129 - Pf1N1B4_2477 0.81 +1.3 2,810,385 - -0.1 2,810,657 - Pf1N1B4_2478 0.12 +0.3 2,810,657 - -0.7 2,810,657 - Pf1N1B4_2478 0.12 -1.0 2,810,749 - Pf1N1B4_2478 0.17 -0.7 2,810,907 + Pf1N1B4_2478 0.24 -2.0 2,810,972 - Pf1N1B4_2478 0.27 -4.5 2,811,296 - +0.3 2,811,388 + Pf1N1B4_2478 0.46 -2.5 2,811,388 + Pf1N1B4_2478 0.46 -1.2 2,811,391 - Pf1N1B4_2478 0.46 -0.7 2,811,391 - Pf1N1B4_2478 0.46 -2.5 2,811,391 - Pf1N1B4_2478 0.46 -3.2 2,811,391 - Pf1N1B4_2478 0.46 -0.7 2,811,439 + Pf1N1B4_2478 0.48 -1.2 2,811,440 - Pf1N1B4_2478 0.48 -2.2 2,811,479 - Pf1N1B4_2478 0.50 -0.7 2,811,489 - Pf1N1B4_2478 0.51 -0.4 2,811,506 - Pf1N1B4_2478 0.51 -2.2 2,811,601 + Pf1N1B4_2478 0.56 -0.7 2,811,603 + Pf1N1B4_2478 0.56 -2.5 2,811,603 + Pf1N1B4_2478 0.56 -0.7 2,811,603 + Pf1N1B4_2478 0.56 -2.0 2,811,603 + Pf1N1B4_2478 0.56 +0.3 2,811,603 + Pf1N1B4_2478 0.56 -2.0 2,811,603 + Pf1N1B4_2478 0.56 -3.1 2,811,603 + Pf1N1B4_2478 0.56 -1.7 2,811,604 - Pf1N1B4_2478 0.56 -2.5 2,811,604 - Pf1N1B4_2478 0.56 +0.3 2,811,604 - Pf1N1B4_2478 0.56 -1.0 2,811,604 - Pf1N1B4_2478 0.56 -2.2 2,811,604 - Pf1N1B4_2478 0.56 -1.2 2,811,604 - Pf1N1B4_2478 0.56 -3.9 2,811,604 - Pf1N1B4_2478 0.56 -1.2 2,811,604 - Pf1N1B4_2478 0.56 -1.2 2,811,699 + Pf1N1B4_2478 0.60 -2.0 2,811,700 - Pf1N1B4_2478 0.60 -0.4 2,811,987 + Pf1N1B4_2478 0.73 -0.7 2,811,987 + Pf1N1B4_2478 0.73 -2.0 2,811,988 - Pf1N1B4_2478 0.74 +0.3 2,811,988 - Pf1N1B4_2478 0.74 -3.1 2,812,086 + Pf1N1B4_2478 0.78 -2.0 2,812,087 - Pf1N1B4_2478 0.78 -0.7 2,812,137 + Pf1N1B4_2478 0.80 -1.7 2,812,137 + Pf1N1B4_2478 0.80 -1.8 2,812,138 - Pf1N1B4_2478 0.80 +0.3 2,812,149 + Pf1N1B4_2478 0.81 -0.2 2,812,149 + Pf1N1B4_2478 0.81 -1.7 2,812,323 + Pf1N1B4_2478 0.89 -1.2 2,812,323 + Pf1N1B4_2478 0.89 +0.3 2,812,323 + Pf1N1B4_2478 0.89 -3.1 2,812,375 + -2.0 2,812,376 - -2.5 2,812,407 - -2.8 2,812,635 + -2.8 2,812,635 + -0.6 2,812,636 - -0.6 2,812,636 - -0.5 2,812,652 + -0.2 2,812,653 - -0.2 2,812,653 - +0.4 2,812,653 - -0.6 2,812,658 + +0.4 2,812,658 + -2.0 2,812,658 + -1.2 2,812,659 - -0.2 2,812,659 - -2.1 2,812,659 - -0.1 2,812,767 + +0.8 2,812,767 + -0.7 2,812,768 - +0.9 2,812,768 - +2.7 2,812,771 + -0.2 2,812,771 + -1.5 2,812,772 - +0.5 2,812,788 + +0.8 2,812,789 - -1.2 2,812,791 + +0.4 2,812,792 - -0.6 2,812,792 - +0.4 2,812,823 + +0.4 2,812,823 + +1.9 2,812,823 + +2.4 2,812,824 - +0.2 2,812,824 - -2.0 2,812,827 + -1.2 2,812,827 + +0.6 2,812,827 + -0.2 2,812,828 - +0.9 2,812,828 - +0.9 2,812,925 + -0.6 2,812,925 + +0.4 2,812,926 - -0.4 2,812,931 + +0.2 2,812,931 + +0.4 2,812,931 + -0.2 2,812,931 + -2.2 2,812,932 - -1.0 2,813,017 - -0.5 2,813,128 + +0.4 2,813,128 + +1.7 2,813,128 + +0.0 2,813,128 + -0.2 2,813,129 - -0.6 2,813,129 - -2.8 2,813,129 - -0.6 2,813,129 - -1.5 2,813,129 - -2.2 2,813,129 - -0.3 2,813,129 - -1.0 2,813,129 - +0.4 2,813,130 + +0.4 2,813,130 + +0.6 2,813,130 + +0.4 2,813,130 + -0.5 2,813,130 + +0.7 2,813,130 + +1.2 2,813,130 + -0.9 2,813,130 + -0.2 2,813,130 + +1.4 2,813,131 - -1.2 2,813,131 - -0.3 2,813,131 - -1.6 2,813,131 - +0.0 2,813,131 - -1.5 2,813,131 - -0.1 2,813,131 - +0.2 2,813,131 - -0.6 2,813,143 - -0.2 2,813,143 - +0.4 2,813,188 - +0.4 2,813,339 + Pf1N1B4_2479 0.32 +0.4 2,813,352 + Pf1N1B4_2479 0.34 -1.6 2,813,353 - Pf1N1B4_2479 0.34 -1.2 2,813,541 + Pf1N1B4_2479 0.71 -0.3 2,813,541 + Pf1N1B4_2479 0.71 -0.6 2,813,542 - Pf1N1B4_2479 0.71 +0.4
Or see this region's nucleotide sequence