Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2469 and Pf1N1B4_2470 are separated by 157 nucleotides Pf1N1B4_2470 and Pf1N1B4_2471 overlap by 4 nucleotides Pf1N1B4_2471 and Pf1N1B4_2472 are separated by 69 nucleotides
Pf1N1B4_2469 - Glutathione S-transferase family protein, at 2,800,239 to 2,800,904
_2469
Pf1N1B4_2470 - FIG00953361: hypothetical protein, at 2,801,062 to 2,801,241
_2470
Pf1N1B4_2471 - putative iron-regulated membrane protein, at 2,801,238 to 2,802,368
_2471
Pf1N1B4_2472 - Ferrichrome-iron receptor @ Ferric siderophore receptor, TonB dependent, at 2,802,438 to 2,804,876
_2472
Position (kb)
2801
2802
2803 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2800.291 kb on + strand at 2800.291 kb on + strand at 2800.292 kb on - strand at 2800.510 kb on - strand, within Pf1N1B4_2469 at 2800.510 kb on - strand, within Pf1N1B4_2469 at 2800.516 kb on + strand, within Pf1N1B4_2469 at 2800.627 kb on + strand, within Pf1N1B4_2469 at 2800.636 kb on + strand, within Pf1N1B4_2469 at 2800.660 kb on + strand, within Pf1N1B4_2469 at 2800.660 kb on + strand at 2800.660 kb on + strand, within Pf1N1B4_2469 at 2800.661 kb on - strand, within Pf1N1B4_2469 at 2800.661 kb on - strand, within Pf1N1B4_2469 at 2800.845 kb on + strand at 2800.845 kb on + strand at 2800.845 kb on + strand at 2800.845 kb on + strand at 2800.845 kb on + strand at 2800.846 kb on - strand at 2801.004 kb on + strand at 2801.004 kb on + strand at 2801.004 kb on + strand at 2801.004 kb on + strand at 2801.005 kb on - strand at 2801.005 kb on - strand at 2801.265 kb on + strand at 2801.266 kb on - strand at 2801.266 kb on - strand at 2801.302 kb on + strand at 2801.302 kb on + strand at 2801.303 kb on - strand at 2801.303 kb on - strand at 2801.303 kb on - strand at 2801.303 kb on - strand at 2801.303 kb on - strand at 2801.303 kb on - strand at 2801.303 kb on - strand at 2801.303 kb on - strand at 2801.422 kb on + strand, within Pf1N1B4_2471 at 2801.422 kb on + strand, within Pf1N1B4_2471 at 2801.422 kb on + strand, within Pf1N1B4_2471 at 2801.423 kb on - strand, within Pf1N1B4_2471 at 2801.423 kb on - strand, within Pf1N1B4_2471 at 2801.617 kb on - strand, within Pf1N1B4_2471 at 2801.617 kb on - strand, within Pf1N1B4_2471 at 2801.713 kb on - strand, within Pf1N1B4_2471 at 2801.713 kb on - strand, within Pf1N1B4_2471 at 2801.713 kb on - strand, within Pf1N1B4_2471 at 2801.870 kb on - strand, within Pf1N1B4_2471 at 2802.131 kb on - strand, within Pf1N1B4_2471 at 2802.448 kb on + strand at 2802.448 kb on + strand at 2802.448 kb on + strand at 2802.448 kb on + strand at 2802.448 kb on + strand at 2802.449 kb on - strand at 2802.449 kb on - strand at 2802.449 kb on - strand at 2802.449 kb on - strand at 2802.449 kb on - strand at 2802.484 kb on + strand at 2802.487 kb on + strand at 2802.488 kb on - strand at 2802.493 kb on + strand at 2802.494 kb on - strand at 2802.494 kb on - strand at 2802.494 kb on - strand at 2802.494 kb on - strand at 2802.517 kb on + strand at 2802.517 kb on + strand at 2802.517 kb on + strand at 2802.517 kb on + strand at 2802.517 kb on + strand at 2802.517 kb on + strand at 2802.518 kb on - strand at 2802.518 kb on - strand at 2802.559 kb on - strand at 2802.559 kb on - strand at 2802.559 kb on - strand at 2802.595 kb on + strand at 2802.613 kb on + strand at 2802.613 kb on + strand at 2802.613 kb on + strand at 2802.613 kb on + strand at 2802.613 kb on + strand at 2802.614 kb on - strand at 2802.614 kb on - strand at 2802.622 kb on + strand at 2802.623 kb on - strand at 2802.623 kb on - strand at 2802.664 kb on + strand at 2802.664 kb on + strand at 2802.664 kb on + strand at 2802.665 kb on - strand at 2802.665 kb on - strand at 2802.680 kb on - strand at 2802.680 kb on - strand at 2802.733 kb on + strand, within Pf1N1B4_2472 at 2802.734 kb on - strand, within Pf1N1B4_2472 at 2802.734 kb on - strand, within Pf1N1B4_2472 at 2802.826 kb on + strand, within Pf1N1B4_2472 at 2802.826 kb on + strand, within Pf1N1B4_2472 at 2802.826 kb on + strand, within Pf1N1B4_2472 at 2802.826 kb on + strand, within Pf1N1B4_2472 at 2802.827 kb on - strand, within Pf1N1B4_2472 at 2802.827 kb on - strand, within Pf1N1B4_2472 at 2802.827 kb on - strand, within Pf1N1B4_2472 at 2802.832 kb on + strand, within Pf1N1B4_2472 at 2802.832 kb on + strand, within Pf1N1B4_2472 at 2802.832 kb on + strand, within Pf1N1B4_2472 at 2802.832 kb on + strand, within Pf1N1B4_2472 at 2802.833 kb on - strand, within Pf1N1B4_2472 at 2802.833 kb on - strand, within Pf1N1B4_2472 at 2802.833 kb on - strand, within Pf1N1B4_2472 at 2802.833 kb on - strand, within Pf1N1B4_2472 at 2803.006 kb on + strand, within Pf1N1B4_2472 at 2803.006 kb on + strand, within Pf1N1B4_2472 at 2803.006 kb on + strand, within Pf1N1B4_2472 at 2803.007 kb on - strand, within Pf1N1B4_2472 at 2803.102 kb on + strand, within Pf1N1B4_2472 at 2803.103 kb on - strand, within Pf1N1B4_2472 at 2803.112 kb on - strand, within Pf1N1B4_2472 at 2803.138 kb on + strand, within Pf1N1B4_2472 at 2803.318 kb on + strand at 2803.319 kb on - strand, within Pf1N1B4_2472 at 2803.324 kb on + strand at 2803.325 kb on - strand, within Pf1N1B4_2472 at 2803.336 kb on + strand, within Pf1N1B4_2472 at 2803.336 kb on + strand, within Pf1N1B4_2472 at 2803.337 kb on - strand, within Pf1N1B4_2472 at 2803.337 kb on - strand, within Pf1N1B4_2472 at 2803.363 kb on + strand, within Pf1N1B4_2472 at 2803.363 kb on + strand, within Pf1N1B4_2472
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 2,800,291 + +1.4 2,800,291 + +0.9 2,800,292 - -0.1 2,800,510 - Pf1N1B4_2469 0.41 -0.6 2,800,510 - Pf1N1B4_2469 0.41 -0.3 2,800,516 + Pf1N1B4_2469 0.42 +2.4 2,800,627 + Pf1N1B4_2469 0.58 +0.2 2,800,636 + Pf1N1B4_2469 0.60 -1.2 2,800,660 + Pf1N1B4_2469 0.63 +1.7 2,800,660 + +0.4 2,800,660 + Pf1N1B4_2469 0.63 +1.4 2,800,661 - Pf1N1B4_2469 0.63 +0.4 2,800,661 - Pf1N1B4_2469 0.63 -2.0 2,800,845 + -0.4 2,800,845 + +1.4 2,800,845 + +0.4 2,800,845 + -1.2 2,800,845 + -0.2 2,800,846 - -0.4 2,801,004 + -2.0 2,801,004 + -0.3 2,801,004 + +0.9 2,801,004 + -1.0 2,801,005 - -1.6 2,801,005 - +0.8 2,801,265 + +1.4 2,801,266 - -1.6 2,801,266 - +0.8 2,801,302 + -0.2 2,801,302 + -1.2 2,801,303 - -1.2 2,801,303 - +0.4 2,801,303 - +0.9 2,801,303 - +1.4 2,801,303 - -2.0 2,801,303 - +0.4 2,801,303 - -0.4 2,801,303 - +0.4 2,801,422 + Pf1N1B4_2471 0.16 +0.4 2,801,422 + Pf1N1B4_2471 0.16 +1.7 2,801,422 + Pf1N1B4_2471 0.16 +0.9 2,801,423 - Pf1N1B4_2471 0.16 +0.1 2,801,423 - Pf1N1B4_2471 0.16 +0.4 2,801,617 - Pf1N1B4_2471 0.34 +0.8 2,801,617 - Pf1N1B4_2471 0.34 +0.4 2,801,713 - Pf1N1B4_2471 0.42 +0.5 2,801,713 - Pf1N1B4_2471 0.42 -0.6 2,801,713 - Pf1N1B4_2471 0.42 -0.6 2,801,870 - Pf1N1B4_2471 0.56 +0.7 2,802,131 - Pf1N1B4_2471 0.79 -0.6 2,802,448 + +1.6 2,802,448 + -0.6 2,802,448 + -1.2 2,802,448 + +0.4 2,802,448 + +0.9 2,802,449 - -1.2 2,802,449 - -0.8 2,802,449 - -1.6 2,802,449 - -0.6 2,802,449 - +0.0 2,802,484 + -0.9 2,802,487 + -0.4 2,802,488 - +0.4 2,802,493 + -0.6 2,802,494 - -0.8 2,802,494 - -1.2 2,802,494 - -1.0 2,802,494 - -1.2 2,802,517 + +0.1 2,802,517 + +0.4 2,802,517 + +0.4 2,802,517 + -0.4 2,802,517 + -1.6 2,802,517 + -1.5 2,802,518 - +0.9 2,802,518 - -1.6 2,802,559 - -0.5 2,802,559 - -2.0 2,802,559 - -1.0 2,802,595 + +0.4 2,802,613 + +0.4 2,802,613 + -2.0 2,802,613 + -0.6 2,802,613 + +1.4 2,802,613 + -0.1 2,802,614 - -0.6 2,802,614 - -0.1 2,802,622 + -0.6 2,802,623 - +0.6 2,802,623 - +0.4 2,802,664 + +1.9 2,802,664 + -1.5 2,802,664 + -0.6 2,802,665 - +0.6 2,802,665 - -0.2 2,802,680 - -0.1 2,802,680 - -1.1 2,802,733 + Pf1N1B4_2472 0.12 +1.9 2,802,734 - Pf1N1B4_2472 0.12 +1.9 2,802,734 - Pf1N1B4_2472 0.12 -0.6 2,802,826 + Pf1N1B4_2472 0.16 -1.0 2,802,826 + Pf1N1B4_2472 0.16 +0.4 2,802,826 + Pf1N1B4_2472 0.16 -1.6 2,802,826 + Pf1N1B4_2472 0.16 +0.5 2,802,827 - Pf1N1B4_2472 0.16 +2.4 2,802,827 - Pf1N1B4_2472 0.16 +2.9 2,802,827 - Pf1N1B4_2472 0.16 -1.0 2,802,832 + Pf1N1B4_2472 0.16 +1.4 2,802,832 + Pf1N1B4_2472 0.16 -0.2 2,802,832 + Pf1N1B4_2472 0.16 +1.7 2,802,832 + Pf1N1B4_2472 0.16 -1.4 2,802,833 - Pf1N1B4_2472 0.16 -0.2 2,802,833 - Pf1N1B4_2472 0.16 -2.4 2,802,833 - Pf1N1B4_2472 0.16 +0.4 2,802,833 - Pf1N1B4_2472 0.16 +0.4 2,803,006 + Pf1N1B4_2472 0.23 -0.6 2,803,006 + Pf1N1B4_2472 0.23 -1.6 2,803,006 + Pf1N1B4_2472 0.23 +0.4 2,803,007 - Pf1N1B4_2472 0.23 -0.6 2,803,102 + Pf1N1B4_2472 0.27 -1.1 2,803,103 - Pf1N1B4_2472 0.27 -0.6 2,803,112 - Pf1N1B4_2472 0.28 -0.4 2,803,138 + Pf1N1B4_2472 0.29 -0.1 2,803,318 + +0.4 2,803,319 - Pf1N1B4_2472 0.36 +2.2 2,803,324 + -1.2 2,803,325 - Pf1N1B4_2472 0.36 -1.4 2,803,336 + Pf1N1B4_2472 0.37 +0.1 2,803,336 + Pf1N1B4_2472 0.37 +0.0 2,803,337 - Pf1N1B4_2472 0.37 +2.4 2,803,337 - Pf1N1B4_2472 0.37 +0.4 2,803,363 + Pf1N1B4_2472 0.38 -1.6 2,803,363 + Pf1N1B4_2472 0.38 -0.6
Or see this region's nucleotide sequence