Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2461 and Pf1N1B4_2462 are separated by 240 nucleotides Pf1N1B4_2462 and Pf1N1B4_2463 are separated by 1 nucleotides Pf1N1B4_2463 and Pf1N1B4_2464 are separated by 273 nucleotides Pf1N1B4_2464 and Pf1N1B4_2465 are separated by 59 nucleotides
Pf1N1B4_2461 - Threonine dehydrogenase and related Zn-dependent dehydrogenases, at 2,791,510 to 2,792,709
_2461
Pf1N1B4_2462 - FIG00955037: hypothetical protein, at 2,792,950 to 2,793,567
_2462
Pf1N1B4_2463 - Acyltransferase family protein, at 2,793,569 to 2,794,453
_2463
Pf1N1B4_2464 - Ribosomal protein S6 glutaminyl transferase, at 2,794,727 to 2,795,233
_2464
Pf1N1B4_2465 - Two-component response regulator CreB, at 2,795,293 to 2,795,973
_2465
Position (kb)
2793
2794
2795 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2792.638 kb on + strand at 2792.707 kb on + strand at 2792.823 kb on + strand at 2792.841 kb on + strand at 2793.312 kb on + strand, within Pf1N1B4_2462 at 2793.478 kb on + strand, within Pf1N1B4_2462 at 2793.479 kb on - strand, within Pf1N1B4_2462 at 2793.479 kb on - strand, within Pf1N1B4_2462 at 2793.557 kb on + strand at 2793.557 kb on + strand at 2793.557 kb on + strand at 2793.558 kb on - strand at 2793.558 kb on - strand at 2793.558 kb on - strand at 2793.558 kb on - strand at 2793.558 kb on - strand at 2793.558 kb on - strand at 2793.561 kb on + strand at 2793.562 kb on - strand at 2793.562 kb on - strand at 2793.669 kb on + strand, within Pf1N1B4_2463 at 2793.912 kb on + strand, within Pf1N1B4_2463 at 2793.930 kb on + strand, within Pf1N1B4_2463 at 2793.931 kb on - strand, within Pf1N1B4_2463 at 2793.931 kb on - strand, within Pf1N1B4_2463 at 2793.931 kb on - strand, within Pf1N1B4_2463 at 2793.931 kb on - strand, within Pf1N1B4_2463 at 2793.960 kb on + strand, within Pf1N1B4_2463 at 2793.960 kb on + strand, within Pf1N1B4_2463 at 2793.960 kb on + strand, within Pf1N1B4_2463 at 2793.961 kb on - strand, within Pf1N1B4_2463 at 2793.961 kb on - strand, within Pf1N1B4_2463 at 2793.961 kb on - strand, within Pf1N1B4_2463 at 2793.984 kb on - strand, within Pf1N1B4_2463 at 2794.057 kb on - strand, within Pf1N1B4_2463 at 2794.057 kb on - strand, within Pf1N1B4_2463 at 2794.203 kb on + strand, within Pf1N1B4_2463 at 2794.203 kb on + strand, within Pf1N1B4_2463 at 2794.203 kb on + strand, within Pf1N1B4_2463 at 2794.203 kb on + strand at 2794.203 kb on + strand, within Pf1N1B4_2463 at 2794.204 kb on - strand, within Pf1N1B4_2463 at 2794.204 kb on - strand, within Pf1N1B4_2463 at 2794.204 kb on - strand, within Pf1N1B4_2463 at 2794.329 kb on + strand, within Pf1N1B4_2463 at 2794.329 kb on + strand, within Pf1N1B4_2463 at 2794.330 kb on - strand, within Pf1N1B4_2463 at 2794.453 kb on + strand at 2794.453 kb on + strand at 2794.453 kb on + strand at 2794.453 kb on + strand at 2794.453 kb on + strand at 2794.453 kb on + strand at 2794.454 kb on - strand at 2794.454 kb on - strand at 2794.454 kb on - strand at 2794.454 kb on - strand at 2794.488 kb on + strand at 2794.489 kb on - strand at 2794.489 kb on - strand at 2794.563 kb on - strand at 2794.690 kb on - strand at 2794.725 kb on - strand at 2794.809 kb on - strand, within Pf1N1B4_2464 at 2794.809 kb on - strand, within Pf1N1B4_2464 at 2794.809 kb on - strand, within Pf1N1B4_2464 at 2794.970 kb on + strand, within Pf1N1B4_2464 at 2794.970 kb on + strand, within Pf1N1B4_2464 at 2794.970 kb on + strand, within Pf1N1B4_2464 at 2794.971 kb on - strand, within Pf1N1B4_2464 at 2794.971 kb on - strand, within Pf1N1B4_2464 at 2795.101 kb on + strand, within Pf1N1B4_2464 at 2795.101 kb on + strand, within Pf1N1B4_2464 at 2795.272 kb on + strand at 2795.272 kb on + strand at 2795.273 kb on - strand at 2795.273 kb on - strand at 2795.273 kb on - strand at 2795.273 kb on - strand at 2795.273 kb on - strand at 2795.276 kb on + strand at 2795.371 kb on - strand, within Pf1N1B4_2465
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 2,792,638 + -1.2 2,792,707 + -0.8 2,792,823 + +1.9 2,792,841 + +0.6 2,793,312 + Pf1N1B4_2462 0.59 +0.8 2,793,478 + Pf1N1B4_2462 0.85 +0.6 2,793,479 - Pf1N1B4_2462 0.86 -0.1 2,793,479 - Pf1N1B4_2462 0.86 +1.9 2,793,557 + +0.8 2,793,557 + +0.9 2,793,557 + -1.2 2,793,558 - -1.0 2,793,558 - +0.4 2,793,558 - -0.6 2,793,558 - -1.2 2,793,558 - +1.4 2,793,558 - -0.6 2,793,561 + -0.4 2,793,562 - +0.4 2,793,562 - +0.4 2,793,669 + Pf1N1B4_2463 0.11 -0.6 2,793,912 + Pf1N1B4_2463 0.39 -0.6 2,793,930 + Pf1N1B4_2463 0.41 -1.2 2,793,931 - Pf1N1B4_2463 0.41 -2.2 2,793,931 - Pf1N1B4_2463 0.41 +1.9 2,793,931 - Pf1N1B4_2463 0.41 +0.7 2,793,931 - Pf1N1B4_2463 0.41 +1.4 2,793,960 + Pf1N1B4_2463 0.44 +1.4 2,793,960 + Pf1N1B4_2463 0.44 -0.2 2,793,960 + Pf1N1B4_2463 0.44 -1.8 2,793,961 - Pf1N1B4_2463 0.44 -0.6 2,793,961 - Pf1N1B4_2463 0.44 -1.4 2,793,961 - Pf1N1B4_2463 0.44 -0.6 2,793,984 - Pf1N1B4_2463 0.47 -1.6 2,794,057 - Pf1N1B4_2463 0.55 -0.6 2,794,057 - Pf1N1B4_2463 0.55 +0.8 2,794,203 + Pf1N1B4_2463 0.72 +0.4 2,794,203 + Pf1N1B4_2463 0.72 -0.6 2,794,203 + Pf1N1B4_2463 0.72 -1.6 2,794,203 + -0.6 2,794,203 + Pf1N1B4_2463 0.72 +1.4 2,794,204 - Pf1N1B4_2463 0.72 -0.3 2,794,204 - Pf1N1B4_2463 0.72 -0.2 2,794,204 - Pf1N1B4_2463 0.72 -0.4 2,794,329 + Pf1N1B4_2463 0.86 +2.4 2,794,329 + Pf1N1B4_2463 0.86 -2.4 2,794,330 - Pf1N1B4_2463 0.86 -0.6 2,794,453 + -1.6 2,794,453 + -1.6 2,794,453 + -0.1 2,794,453 + +0.4 2,794,453 + -1.6 2,794,453 + -0.1 2,794,454 - +0.4 2,794,454 - -1.0 2,794,454 - +1.9 2,794,454 - +0.4 2,794,488 + +0.4 2,794,489 - -0.1 2,794,489 - -0.6 2,794,563 - +0.9 2,794,690 - -0.6 2,794,725 - +0.4 2,794,809 - Pf1N1B4_2464 0.16 -0.2 2,794,809 - Pf1N1B4_2464 0.16 +1.9 2,794,809 - Pf1N1B4_2464 0.16 -0.6 2,794,970 + Pf1N1B4_2464 0.48 -1.2 2,794,970 + Pf1N1B4_2464 0.48 +2.4 2,794,970 + Pf1N1B4_2464 0.48 +1.9 2,794,971 - Pf1N1B4_2464 0.48 -0.4 2,794,971 - Pf1N1B4_2464 0.48 +0.1 2,795,101 + Pf1N1B4_2464 0.74 +1.4 2,795,101 + Pf1N1B4_2464 0.74 -1.2 2,795,272 + +0.4 2,795,272 + +0.9 2,795,273 - -1.0 2,795,273 - +0.4 2,795,273 - +1.8 2,795,273 - -0.4 2,795,273 - +2.4 2,795,276 + -2.0 2,795,371 - Pf1N1B4_2465 0.11 -2.5
Or see this region's nucleotide sequence