Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2457 and Pf1N1B4_2458 are separated by 129 nucleotides Pf1N1B4_2458 and Pf1N1B4_2459 overlap by 17 nucleotides Pf1N1B4_2459 and Pf1N1B4_2460 are separated by 6 nucleotides
Pf1N1B4_2457 - Sarcosine oxidase alpha subunit (EC 1.5.3.1), at 2,786,534 to 2,789,551
_2457
Pf1N1B4_2458 - Sarcosine oxidase gamma subunit (EC 1.5.3.1), at 2,789,681 to 2,790,313
_2458
Pf1N1B4_2459 - hypothetical protein, at 2,790,297 to 2,790,440
_2459
Pf1N1B4_2460 - Formyltetrahydrofolate deformylase (EC 3.5.1.10), at 2,790,447 to 2,791,304
_2460
Position (kb)
2790
2791 Strain fitness (log2 ratio)
-2
-1
0
1 at 2789.549 kb on + strand at 2789.549 kb on + strand at 2789.550 kb on - strand at 2789.550 kb on - strand at 2789.680 kb on + strand at 2789.681 kb on - strand at 2790.096 kb on + strand, within Pf1N1B4_2458 at 2790.097 kb on - strand, within Pf1N1B4_2458 at 2790.125 kb on + strand, within Pf1N1B4_2458 at 2790.125 kb on + strand, within Pf1N1B4_2458 at 2790.125 kb on + strand, within Pf1N1B4_2458 at 2790.126 kb on - strand, within Pf1N1B4_2458 at 2790.126 kb on - strand, within Pf1N1B4_2458 at 2790.126 kb on - strand, within Pf1N1B4_2458 at 2790.142 kb on + strand, within Pf1N1B4_2458 at 2790.298 kb on + strand at 2790.312 kb on - strand at 2790.443 kb on + strand at 2790.444 kb on - strand at 2790.444 kb on - strand at 2790.446 kb on + strand at 2790.446 kb on + strand at 2790.446 kb on + strand at 2790.546 kb on + strand, within Pf1N1B4_2460 at 2790.547 kb on - strand, within Pf1N1B4_2460 at 2790.547 kb on - strand, within Pf1N1B4_2460 at 2790.722 kb on - strand, within Pf1N1B4_2460 at 2790.868 kb on - strand, within Pf1N1B4_2460 at 2790.966 kb on + strand, within Pf1N1B4_2460 at 2790.966 kb on + strand, within Pf1N1B4_2460 at 2790.966 kb on + strand, within Pf1N1B4_2460 at 2790.966 kb on + strand, within Pf1N1B4_2460 at 2790.967 kb on - strand, within Pf1N1B4_2460 at 2791.077 kb on + strand, within Pf1N1B4_2460 at 2791.078 kb on - strand, within Pf1N1B4_2460 at 2791.116 kb on + strand, within Pf1N1B4_2460 at 2791.116 kb on + strand, within Pf1N1B4_2460 at 2791.116 kb on + strand, within Pf1N1B4_2460 at 2791.116 kb on + strand, within Pf1N1B4_2460 at 2791.117 kb on - strand, within Pf1N1B4_2460 at 2791.117 kb on - strand, within Pf1N1B4_2460 at 2791.117 kb on - strand, within Pf1N1B4_2460 at 2791.117 kb on - strand, within Pf1N1B4_2460 at 2791.178 kb on + strand, within Pf1N1B4_2460 at 2791.179 kb on - strand, within Pf1N1B4_2460 at 2791.179 kb on - strand, within Pf1N1B4_2460 at 2791.179 kb on - strand, within Pf1N1B4_2460 at 2791.179 kb on - strand, within Pf1N1B4_2460 at 2791.179 kb on - strand, within Pf1N1B4_2460 at 2791.179 kb on - strand, within Pf1N1B4_2460 at 2791.302 kb on + strand at 2791.303 kb on - strand at 2791.363 kb on + strand at 2791.364 kb on - strand at 2791.364 kb on - strand at 2791.364 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 2,789,549 + -0.2 2,789,549 + +1.1 2,789,550 - +0.4 2,789,550 - -0.2 2,789,680 + -1.2 2,789,681 - -0.2 2,790,096 + Pf1N1B4_2458 0.66 +0.4 2,790,097 - Pf1N1B4_2458 0.66 -1.4 2,790,125 + Pf1N1B4_2458 0.70 -1.0 2,790,125 + Pf1N1B4_2458 0.70 -0.0 2,790,125 + Pf1N1B4_2458 0.70 +1.4 2,790,126 - Pf1N1B4_2458 0.70 +1.1 2,790,126 - Pf1N1B4_2458 0.70 -2.0 2,790,126 - Pf1N1B4_2458 0.70 -2.0 2,790,142 + Pf1N1B4_2458 0.73 -0.0 2,790,298 + -0.4 2,790,312 - -0.4 2,790,443 + -0.6 2,790,444 - -2.0 2,790,444 - +0.5 2,790,446 + -2.0 2,790,446 + -0.0 2,790,446 + +1.0 2,790,546 + Pf1N1B4_2460 0.12 -0.6 2,790,547 - Pf1N1B4_2460 0.12 -1.6 2,790,547 - Pf1N1B4_2460 0.12 -1.4 2,790,722 - Pf1N1B4_2460 0.32 -0.0 2,790,868 - Pf1N1B4_2460 0.49 -0.6 2,790,966 + Pf1N1B4_2460 0.60 +0.7 2,790,966 + Pf1N1B4_2460 0.60 -0.6 2,790,966 + Pf1N1B4_2460 0.60 +1.4 2,790,966 + Pf1N1B4_2460 0.60 +1.4 2,790,967 - Pf1N1B4_2460 0.61 -1.5 2,791,077 + Pf1N1B4_2460 0.73 -0.6 2,791,078 - Pf1N1B4_2460 0.74 -1.0 2,791,116 + Pf1N1B4_2460 0.78 -1.6 2,791,116 + Pf1N1B4_2460 0.78 +0.1 2,791,116 + Pf1N1B4_2460 0.78 -2.2 2,791,116 + Pf1N1B4_2460 0.78 -0.6 2,791,117 - Pf1N1B4_2460 0.78 -1.2 2,791,117 - Pf1N1B4_2460 0.78 +0.4 2,791,117 - Pf1N1B4_2460 0.78 +0.1 2,791,117 - Pf1N1B4_2460 0.78 -0.9 2,791,178 + Pf1N1B4_2460 0.85 +0.6 2,791,179 - Pf1N1B4_2460 0.85 +1.0 2,791,179 - Pf1N1B4_2460 0.85 -0.6 2,791,179 - Pf1N1B4_2460 0.85 +1.5 2,791,179 - Pf1N1B4_2460 0.85 -2.1 2,791,179 - Pf1N1B4_2460 0.85 +0.4 2,791,179 - Pf1N1B4_2460 0.85 -1.2 2,791,302 + -1.1 2,791,303 - -1.6 2,791,363 + -1.6 2,791,364 - +1.0 2,791,364 - +1.1 2,791,364 - -0.6
Or see this region's nucleotide sequence