Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2432 and Pf1N1B4_2433 are separated by 137 nucleotides Pf1N1B4_2433 and Pf1N1B4_2434 are separated by 32 nucleotides Pf1N1B4_2434 and Pf1N1B4_2435 are separated by 40 nucleotides Pf1N1B4_2435 and Pf1N1B4_2436 are separated by 306 nucleotides
Pf1N1B4_2432 - Transcriptional regulator containing an amidase domain and an AraC-type DNA-binding HTH domain, at 2,757,405 to 2,758,349
_2432
Pf1N1B4_2433 - FIG00953108: hypothetical protein, at 2,758,487 to 2,758,903
_2433
Pf1N1B4_2434 - putative DNA binding protein, at 2,758,936 to 2,759,202
_2434
Pf1N1B4_2435 - hypothetical protein, at 2,759,243 to 2,759,395
_2435
Pf1N1B4_2436 - FIG00953563: hypothetical protein, at 2,759,702 to 2,760,103
_2436
Position (kb)
2758
2759 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2757.510 kb on + strand, within Pf1N1B4_2432 at 2757.619 kb on + strand, within Pf1N1B4_2432 at 2757.811 kb on - strand, within Pf1N1B4_2432 at 2758.088 kb on + strand, within Pf1N1B4_2432 at 2758.132 kb on + strand, within Pf1N1B4_2432 at 2758.132 kb on + strand, within Pf1N1B4_2432 at 2758.180 kb on - strand, within Pf1N1B4_2432 at 2758.279 kb on - strand at 2758.348 kb on - strand at 2758.488 kb on + strand at 2758.489 kb on - strand at 2758.521 kb on + strand at 2758.522 kb on - strand at 2758.522 kb on - strand at 2758.522 kb on - strand at 2758.537 kb on - strand, within Pf1N1B4_2433 at 2758.537 kb on - strand, within Pf1N1B4_2433 at 2758.660 kb on + strand, within Pf1N1B4_2433 at 2758.660 kb on + strand, within Pf1N1B4_2433 at 2758.661 kb on - strand, within Pf1N1B4_2433 at 2758.661 kb on - strand, within Pf1N1B4_2433 at 2758.661 kb on - strand, within Pf1N1B4_2433 at 2758.661 kb on - strand, within Pf1N1B4_2433 at 2758.937 kb on + strand at 2758.937 kb on + strand at 2758.937 kb on + strand at 2758.938 kb on - strand at 2758.938 kb on - strand at 2758.945 kb on + strand at 2758.945 kb on + strand at 2758.945 kb on + strand at 2759.076 kb on + strand, within Pf1N1B4_2434 at 2759.077 kb on - strand, within Pf1N1B4_2434 at 2759.180 kb on + strand at 2759.180 kb on + strand at 2759.180 kb on + strand at 2759.181 kb on - strand at 2759.181 kb on - strand at 2759.181 kb on - strand at 2759.181 kb on - strand at 2759.181 kb on - strand at 2759.247 kb on + strand at 2759.248 kb on - strand at 2759.248 kb on - strand at 2759.248 kb on - strand at 2759.248 kb on - strand at 2759.281 kb on + strand, within Pf1N1B4_2435 at 2759.317 kb on + strand, within Pf1N1B4_2435 at 2759.317 kb on + strand, within Pf1N1B4_2435 at 2759.329 kb on + strand, within Pf1N1B4_2435 at 2759.352 kb on + strand, within Pf1N1B4_2435 at 2759.353 kb on - strand, within Pf1N1B4_2435 at 2759.384 kb on + strand at 2759.384 kb on + strand at 2759.384 kb on + strand at 2759.384 kb on + strand at 2759.384 kb on + strand at 2759.384 kb on + strand at 2759.385 kb on - strand at 2759.503 kb on + strand at 2759.590 kb on + strand at 2759.622 kb on + strand at 2759.659 kb on + strand at 2759.660 kb on - strand at 2759.670 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 2,757,510 + Pf1N1B4_2432 0.11 +0.4 2,757,619 + Pf1N1B4_2432 0.23 -0.0 2,757,811 - Pf1N1B4_2432 0.43 +0.1 2,758,088 + Pf1N1B4_2432 0.72 -1.6 2,758,132 + Pf1N1B4_2432 0.77 +0.4 2,758,132 + Pf1N1B4_2432 0.77 -0.6 2,758,180 - Pf1N1B4_2432 0.82 +1.1 2,758,279 - +0.4 2,758,348 - -2.2 2,758,488 + -0.6 2,758,489 - -0.9 2,758,521 + +0.8 2,758,522 - +1.4 2,758,522 - -0.6 2,758,522 - +0.4 2,758,537 - Pf1N1B4_2433 0.12 +1.0 2,758,537 - Pf1N1B4_2433 0.12 +1.0 2,758,660 + Pf1N1B4_2433 0.41 +1.4 2,758,660 + Pf1N1B4_2433 0.41 -0.6 2,758,661 - Pf1N1B4_2433 0.42 -0.0 2,758,661 - Pf1N1B4_2433 0.42 +1.0 2,758,661 - Pf1N1B4_2433 0.42 -1.2 2,758,661 - Pf1N1B4_2433 0.42 +0.5 2,758,937 + -0.9 2,758,937 + +1.1 2,758,937 + +1.2 2,758,938 - +0.4 2,758,938 - +0.1 2,758,945 + -0.9 2,758,945 + -1.9 2,758,945 + +0.1 2,759,076 + Pf1N1B4_2434 0.52 +0.6 2,759,077 - Pf1N1B4_2434 0.53 -0.2 2,759,180 + +0.1 2,759,180 + -1.4 2,759,180 + +2.0 2,759,181 - +0.8 2,759,181 - +0.5 2,759,181 - +0.7 2,759,181 - +0.4 2,759,181 - -0.6 2,759,247 + -0.6 2,759,248 - -0.6 2,759,248 - +1.4 2,759,248 - +0.4 2,759,248 - -1.4 2,759,281 + Pf1N1B4_2435 0.25 -0.6 2,759,317 + Pf1N1B4_2435 0.48 -0.1 2,759,317 + Pf1N1B4_2435 0.48 -0.9 2,759,329 + Pf1N1B4_2435 0.56 -0.4 2,759,352 + Pf1N1B4_2435 0.71 -0.6 2,759,353 - Pf1N1B4_2435 0.72 -1.3 2,759,384 + -1.2 2,759,384 + +0.4 2,759,384 + -1.2 2,759,384 + -1.4 2,759,384 + -0.2 2,759,384 + -0.4 2,759,385 - -1.6 2,759,503 + -0.2 2,759,590 + -1.2 2,759,622 + -0.4 2,759,659 + -0.0 2,759,660 - -1.2 2,759,670 - +1.0
Or see this region's nucleotide sequence