Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2383 and Pf1N1B4_2384 are separated by 165 nucleotides Pf1N1B4_2384 and Pf1N1B4_2385 are separated by 160 nucleotides
Pf1N1B4_2383 - periplasmic binding protein, putative, at 2,700,552 to 2,701,394
_2383
Pf1N1B4_2384 - 5,10-methylenetetrahydrofolate reductase (EC 1.5.1.20), at 2,701,560 to 2,702,405
_2384
Pf1N1B4_2385 - Adenosylhomocysteinase (EC 3.3.1.1), at 2,702,566 to 2,703,975
_2385
Position (kb)
2701
2702
2703 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2700.741 kb on + strand, within Pf1N1B4_2383 at 2700.949 kb on + strand, within Pf1N1B4_2383 at 2700.950 kb on - strand, within Pf1N1B4_2383 at 2700.955 kb on + strand, within Pf1N1B4_2383 at 2701.030 kb on + strand, within Pf1N1B4_2383 at 2701.030 kb on + strand, within Pf1N1B4_2383 at 2701.030 kb on + strand, within Pf1N1B4_2383 at 2701.030 kb on + strand, within Pf1N1B4_2383 at 2701.031 kb on - strand, within Pf1N1B4_2383 at 2701.031 kb on - strand at 2701.038 kb on + strand, within Pf1N1B4_2383 at 2701.072 kb on + strand, within Pf1N1B4_2383 at 2701.110 kb on - strand, within Pf1N1B4_2383 at 2701.258 kb on + strand, within Pf1N1B4_2383 at 2701.258 kb on + strand, within Pf1N1B4_2383 at 2701.258 kb on + strand, within Pf1N1B4_2383 at 2701.259 kb on - strand, within Pf1N1B4_2383 at 2701.259 kb on - strand, within Pf1N1B4_2383 at 2701.259 kb on - strand, within Pf1N1B4_2383 at 2701.259 kb on - strand, within Pf1N1B4_2383 at 2701.264 kb on + strand, within Pf1N1B4_2383 at 2701.264 kb on + strand, within Pf1N1B4_2383 at 2701.264 kb on + strand, within Pf1N1B4_2383 at 2701.264 kb on + strand, within Pf1N1B4_2383 at 2701.264 kb on + strand, within Pf1N1B4_2383 at 2701.264 kb on + strand, within Pf1N1B4_2383 at 2701.265 kb on - strand, within Pf1N1B4_2383 at 2701.265 kb on - strand, within Pf1N1B4_2383 at 2701.265 kb on - strand, within Pf1N1B4_2383 at 2701.265 kb on - strand, within Pf1N1B4_2383 at 2701.265 kb on - strand, within Pf1N1B4_2383 at 2701.347 kb on + strand at 2701.379 kb on + strand at 2701.394 kb on + strand at 2701.395 kb on - strand at 2701.435 kb on - strand at 2701.447 kb on + strand at 2701.447 kb on + strand at 2701.448 kb on - strand at 2701.448 kb on - strand at 2701.501 kb on + strand at 2701.502 kb on - strand at 2701.506 kb on - strand at 2701.506 kb on - strand at 2701.513 kb on + strand at 2701.621 kb on + strand at 2701.622 kb on - strand at 2701.622 kb on - strand at 2701.721 kb on - strand, within Pf1N1B4_2384 at 2701.795 kb on + strand, within Pf1N1B4_2384 at 2701.795 kb on + strand, within Pf1N1B4_2384 at 2701.795 kb on + strand, within Pf1N1B4_2384 at 2701.795 kb on + strand, within Pf1N1B4_2384 at 2701.795 kb on + strand, within Pf1N1B4_2384 at 2701.795 kb on + strand, within Pf1N1B4_2384 at 2701.795 kb on + strand, within Pf1N1B4_2384 at 2701.795 kb on + strand, within Pf1N1B4_2384 at 2701.796 kb on - strand, within Pf1N1B4_2384 at 2701.796 kb on - strand, within Pf1N1B4_2384 at 2701.796 kb on - strand, within Pf1N1B4_2384 at 2701.796 kb on - strand, within Pf1N1B4_2384 at 2701.796 kb on - strand, within Pf1N1B4_2384 at 2701.796 kb on - strand, within Pf1N1B4_2384 at 2701.796 kb on - strand, within Pf1N1B4_2384 at 2701.867 kb on + strand, within Pf1N1B4_2384 at 2701.867 kb on + strand, within Pf1N1B4_2384 at 2701.868 kb on - strand, within Pf1N1B4_2384 at 2701.868 kb on - strand, within Pf1N1B4_2384 at 2701.868 kb on - strand, within Pf1N1B4_2384 at 2701.868 kb on - strand, within Pf1N1B4_2384 at 2701.868 kb on - strand, within Pf1N1B4_2384 at 2701.873 kb on + strand, within Pf1N1B4_2384 at 2701.873 kb on + strand, within Pf1N1B4_2384 at 2701.873 kb on + strand, within Pf1N1B4_2384 at 2701.873 kb on + strand, within Pf1N1B4_2384 at 2701.873 kb on + strand, within Pf1N1B4_2384 at 2701.873 kb on + strand, within Pf1N1B4_2384 at 2701.873 kb on + strand, within Pf1N1B4_2384 at 2701.873 kb on + strand, within Pf1N1B4_2384 at 2701.873 kb on + strand, within Pf1N1B4_2384 at 2701.873 kb on + strand, within Pf1N1B4_2384 at 2701.873 kb on + strand, within Pf1N1B4_2384 at 2701.873 kb on + strand, within Pf1N1B4_2384 at 2701.873 kb on + strand, within Pf1N1B4_2384 at 2701.873 kb on + strand, within Pf1N1B4_2384 at 2701.874 kb on - strand, within Pf1N1B4_2384 at 2701.874 kb on - strand, within Pf1N1B4_2384 at 2701.874 kb on - strand, within Pf1N1B4_2384 at 2701.874 kb on - strand, within Pf1N1B4_2384 at 2701.874 kb on - strand, within Pf1N1B4_2384 at 2701.874 kb on - strand, within Pf1N1B4_2384 at 2701.874 kb on - strand, within Pf1N1B4_2384 at 2701.874 kb on - strand, within Pf1N1B4_2384 at 2701.874 kb on - strand, within Pf1N1B4_2384 at 2701.894 kb on + strand, within Pf1N1B4_2384 at 2701.894 kb on + strand, within Pf1N1B4_2384 at 2701.894 kb on + strand, within Pf1N1B4_2384 at 2701.894 kb on + strand, within Pf1N1B4_2384 at 2701.895 kb on - strand, within Pf1N1B4_2384 at 2701.895 kb on - strand, within Pf1N1B4_2384 at 2701.895 kb on - strand, within Pf1N1B4_2384 at 2702.120 kb on + strand, within Pf1N1B4_2384 at 2702.120 kb on + strand, within Pf1N1B4_2384 at 2702.121 kb on - strand, within Pf1N1B4_2384 at 2702.143 kb on + strand, within Pf1N1B4_2384 at 2702.143 kb on + strand, within Pf1N1B4_2384 at 2702.143 kb on + strand, within Pf1N1B4_2384 at 2702.143 kb on + strand, within Pf1N1B4_2384 at 2702.143 kb on + strand, within Pf1N1B4_2384 at 2702.143 kb on + strand, within Pf1N1B4_2384 at 2702.144 kb on - strand, within Pf1N1B4_2384 at 2702.144 kb on - strand, within Pf1N1B4_2384 at 2702.144 kb on - strand, within Pf1N1B4_2384 at 2702.144 kb on - strand, within Pf1N1B4_2384 at 2702.144 kb on - strand, within Pf1N1B4_2384 at 2702.144 kb on - strand, within Pf1N1B4_2384 at 2702.144 kb on - strand, within Pf1N1B4_2384 at 2702.144 kb on - strand, within Pf1N1B4_2384 at 2702.144 kb on - strand, within Pf1N1B4_2384 at 2702.282 kb on - strand, within Pf1N1B4_2384 at 2702.387 kb on - strand at 2702.411 kb on + strand at 2702.412 kb on - strand at 2702.427 kb on + strand at 2702.427 kb on + strand at 2702.427 kb on + strand at 2702.427 kb on + strand at 2702.427 kb on + strand at 2702.428 kb on - strand at 2702.428 kb on - strand at 2702.496 kb on + strand at 2702.496 kb on + strand at 2702.496 kb on + strand at 2702.497 kb on - strand at 2702.497 kb on - strand at 2702.509 kb on + strand at 2702.509 kb on + strand at 2702.509 kb on + strand at 2702.509 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 2,700,741 + Pf1N1B4_2383 0.22 +1.7 2,700,949 + Pf1N1B4_2383 0.47 -0.2 2,700,950 - Pf1N1B4_2383 0.47 -1.4 2,700,955 + Pf1N1B4_2383 0.48 -1.2 2,701,030 + Pf1N1B4_2383 0.57 -2.2 2,701,030 + Pf1N1B4_2383 0.57 -0.8 2,701,030 + Pf1N1B4_2383 0.57 +0.4 2,701,030 + Pf1N1B4_2383 0.57 +1.4 2,701,031 - Pf1N1B4_2383 0.57 -1.2 2,701,031 - +2.4 2,701,038 + Pf1N1B4_2383 0.58 -1.6 2,701,072 + Pf1N1B4_2383 0.62 +0.0 2,701,110 - Pf1N1B4_2383 0.66 +0.4 2,701,258 + Pf1N1B4_2383 0.84 -0.7 2,701,258 + Pf1N1B4_2383 0.84 +0.4 2,701,258 + Pf1N1B4_2383 0.84 -1.9 2,701,259 - Pf1N1B4_2383 0.84 +0.4 2,701,259 - Pf1N1B4_2383 0.84 +1.0 2,701,259 - Pf1N1B4_2383 0.84 -1.9 2,701,259 - Pf1N1B4_2383 0.84 +0.4 2,701,264 + Pf1N1B4_2383 0.84 -0.6 2,701,264 + Pf1N1B4_2383 0.84 -1.6 2,701,264 + Pf1N1B4_2383 0.84 +0.4 2,701,264 + Pf1N1B4_2383 0.84 +0.1 2,701,264 + Pf1N1B4_2383 0.84 -0.4 2,701,264 + Pf1N1B4_2383 0.84 -0.6 2,701,265 - Pf1N1B4_2383 0.85 +0.4 2,701,265 - Pf1N1B4_2383 0.85 -0.1 2,701,265 - Pf1N1B4_2383 0.85 +1.2 2,701,265 - Pf1N1B4_2383 0.85 -0.6 2,701,265 - Pf1N1B4_2383 0.85 +1.1 2,701,347 + -0.6 2,701,379 + -0.2 2,701,394 + -1.6 2,701,395 - +1.0 2,701,435 - -0.0 2,701,447 + -0.2 2,701,447 + -1.6 2,701,448 - -0.6 2,701,448 - +0.6 2,701,501 + +0.4 2,701,502 - +2.0 2,701,506 - -0.0 2,701,506 - -0.6 2,701,513 + -0.2 2,701,621 + -1.6 2,701,622 - -0.0 2,701,622 - +1.1 2,701,721 - Pf1N1B4_2384 0.19 -2.4 2,701,795 + Pf1N1B4_2384 0.28 -1.9 2,701,795 + Pf1N1B4_2384 0.28 -2.9 2,701,795 + Pf1N1B4_2384 0.28 -2.4 2,701,795 + Pf1N1B4_2384 0.28 -2.9 2,701,795 + Pf1N1B4_2384 0.28 -2.4 2,701,795 + Pf1N1B4_2384 0.28 -2.9 2,701,795 + Pf1N1B4_2384 0.28 -1.6 2,701,795 + Pf1N1B4_2384 0.28 -1.6 2,701,796 - Pf1N1B4_2384 0.28 -0.6 2,701,796 - Pf1N1B4_2384 0.28 -1.9 2,701,796 - Pf1N1B4_2384 0.28 -0.6 2,701,796 - Pf1N1B4_2384 0.28 -3.2 2,701,796 - Pf1N1B4_2384 0.28 -2.4 2,701,796 - Pf1N1B4_2384 0.28 -1.9 2,701,796 - Pf1N1B4_2384 0.28 -3.1 2,701,867 + Pf1N1B4_2384 0.36 -2.4 2,701,867 + Pf1N1B4_2384 0.36 -1.9 2,701,868 - Pf1N1B4_2384 0.36 -2.1 2,701,868 - Pf1N1B4_2384 0.36 -3.5 2,701,868 - Pf1N1B4_2384 0.36 -1.9 2,701,868 - Pf1N1B4_2384 0.36 +0.4 2,701,868 - Pf1N1B4_2384 0.36 -1.6 2,701,873 + Pf1N1B4_2384 0.37 -0.0 2,701,873 + Pf1N1B4_2384 0.37 -3.1 2,701,873 + Pf1N1B4_2384 0.37 -1.2 2,701,873 + Pf1N1B4_2384 0.37 -4.5 2,701,873 + Pf1N1B4_2384 0.37 -2.8 2,701,873 + Pf1N1B4_2384 0.37 -0.6 2,701,873 + Pf1N1B4_2384 0.37 -0.0 2,701,873 + Pf1N1B4_2384 0.37 -2.4 2,701,873 + Pf1N1B4_2384 0.37 -2.1 2,701,873 + Pf1N1B4_2384 0.37 -2.4 2,701,873 + Pf1N1B4_2384 0.37 -2.9 2,701,873 + Pf1N1B4_2384 0.37 -2.2 2,701,873 + Pf1N1B4_2384 0.37 -2.6 2,701,873 + Pf1N1B4_2384 0.37 +0.4 2,701,874 - Pf1N1B4_2384 0.37 -2.6 2,701,874 - Pf1N1B4_2384 0.37 -3.1 2,701,874 - Pf1N1B4_2384 0.37 -2.2 2,701,874 - Pf1N1B4_2384 0.37 -2.4 2,701,874 - Pf1N1B4_2384 0.37 -2.2 2,701,874 - Pf1N1B4_2384 0.37 +0.4 2,701,874 - Pf1N1B4_2384 0.37 +0.3 2,701,874 - Pf1N1B4_2384 0.37 -2.6 2,701,874 - Pf1N1B4_2384 0.37 -0.6 2,701,894 + Pf1N1B4_2384 0.39 -0.6 2,701,894 + Pf1N1B4_2384 0.39 -2.8 2,701,894 + Pf1N1B4_2384 0.39 -2.4 2,701,894 + Pf1N1B4_2384 0.39 +0.4 2,701,895 - Pf1N1B4_2384 0.40 -1.6 2,701,895 - Pf1N1B4_2384 0.40 -1.2 2,701,895 - Pf1N1B4_2384 0.40 -2.4 2,702,120 + Pf1N1B4_2384 0.66 -2.8 2,702,120 + Pf1N1B4_2384 0.66 -1.9 2,702,121 - Pf1N1B4_2384 0.66 -1.2 2,702,143 + Pf1N1B4_2384 0.69 -1.9 2,702,143 + Pf1N1B4_2384 0.69 -2.8 2,702,143 + Pf1N1B4_2384 0.69 +1.4 2,702,143 + Pf1N1B4_2384 0.69 -3.1 2,702,143 + Pf1N1B4_2384 0.69 -1.2 2,702,143 + Pf1N1B4_2384 0.69 -2.8 2,702,144 - Pf1N1B4_2384 0.69 -2.8 2,702,144 - Pf1N1B4_2384 0.69 -3.2 2,702,144 - Pf1N1B4_2384 0.69 -0.6 2,702,144 - Pf1N1B4_2384 0.69 -2.2 2,702,144 - Pf1N1B4_2384 0.69 -1.2 2,702,144 - Pf1N1B4_2384 0.69 -2.6 2,702,144 - Pf1N1B4_2384 0.69 -2.2 2,702,144 - Pf1N1B4_2384 0.69 -2.4 2,702,144 - Pf1N1B4_2384 0.69 -3.6 2,702,282 - Pf1N1B4_2384 0.85 -1.9 2,702,387 - -1.2 2,702,411 + -2.4 2,702,412 - -1.0 2,702,427 + -2.9 2,702,427 + -2.6 2,702,427 + -2.2 2,702,427 + -2.2 2,702,427 + -2.6 2,702,428 - -0.0 2,702,428 - +0.4 2,702,496 + -1.4 2,702,496 + +0.4 2,702,496 + -2.6 2,702,497 - +1.0 2,702,497 - +2.4 2,702,509 + -1.2 2,702,509 + -1.2 2,702,509 + -1.9 2,702,509 + -2.2
Or see this region's nucleotide sequence