Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2375 and Pf1N1B4_2376 are separated by 124 nucleotides Pf1N1B4_2376 and Pf1N1B4_2377 are separated by 138 nucleotides Pf1N1B4_2377 and Pf1N1B4_2378 are separated by 222 nucleotides
Pf1N1B4_2375 - Glutamate Aspartate periplasmic binding protein precursor GltI (TC 3.A.1.3.4), at 2,690,454 to 2,691,356
_2375
Pf1N1B4_2376 - Ribosomal RNA small subunit methyltransferase E (EC 2.1.1.-), at 2,691,481 to 2,692,200
_2376
Pf1N1B4_2377 - Adenosylmethionine-8-amino-7-oxononanoate aminotransferase (EC 2.6.1.62), at 2,692,339 to 2,693,745
_2377
Pf1N1B4_2378 - Amine oxidase [flavin-containing] (EC 1.4.3.4), at 2,693,968 to 2,695,743
_2378
Position (kb)
2692
2693
2694 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2691.354 kb on + strand at 2691.354 kb on + strand at 2691.354 kb on + strand at 2691.354 kb on + strand at 2691.355 kb on - strand at 2691.355 kb on - strand at 2692.124 kb on + strand, within Pf1N1B4_2376 at 2692.124 kb on + strand, within Pf1N1B4_2376 at 2692.125 kb on - strand, within Pf1N1B4_2376 at 2692.125 kb on - strand at 2692.213 kb on + strand at 2692.213 kb on + strand at 2692.214 kb on - strand at 2692.214 kb on - strand at 2692.214 kb on - strand at 2692.214 kb on - strand at 2692.470 kb on - strand at 2692.470 kb on - strand at 2692.484 kb on + strand, within Pf1N1B4_2377 at 2692.485 kb on - strand, within Pf1N1B4_2377 at 2692.485 kb on - strand, within Pf1N1B4_2377 at 2692.485 kb on - strand, within Pf1N1B4_2377 at 2692.781 kb on + strand, within Pf1N1B4_2377 at 2692.781 kb on + strand, within Pf1N1B4_2377 at 2692.782 kb on - strand, within Pf1N1B4_2377 at 2692.826 kb on + strand, within Pf1N1B4_2377 at 2692.826 kb on + strand, within Pf1N1B4_2377 at 2692.826 kb on + strand, within Pf1N1B4_2377 at 2692.839 kb on - strand, within Pf1N1B4_2377 at 2692.839 kb on - strand, within Pf1N1B4_2377 at 2692.877 kb on + strand, within Pf1N1B4_2377 at 2693.036 kb on + strand, within Pf1N1B4_2377 at 2693.037 kb on - strand, within Pf1N1B4_2377 at 2693.048 kb on + strand, within Pf1N1B4_2377 at 2693.048 kb on + strand, within Pf1N1B4_2377 at 2693.048 kb on + strand, within Pf1N1B4_2377 at 2693.048 kb on + strand, within Pf1N1B4_2377 at 2693.048 kb on + strand, within Pf1N1B4_2377 at 2693.048 kb on + strand, within Pf1N1B4_2377 at 2693.049 kb on - strand, within Pf1N1B4_2377 at 2693.192 kb on + strand, within Pf1N1B4_2377 at 2693.193 kb on - strand, within Pf1N1B4_2377 at 2693.240 kb on + strand, within Pf1N1B4_2377 at 2693.240 kb on + strand, within Pf1N1B4_2377 at 2693.240 kb on + strand, within Pf1N1B4_2377 at 2693.240 kb on + strand, within Pf1N1B4_2377 at 2693.241 kb on - strand, within Pf1N1B4_2377 at 2693.251 kb on - strand, within Pf1N1B4_2377 at 2693.411 kb on + strand, within Pf1N1B4_2377 at 2693.411 kb on + strand, within Pf1N1B4_2377 at 2693.411 kb on + strand, within Pf1N1B4_2377 at 2693.412 kb on - strand, within Pf1N1B4_2377 at 2693.412 kb on - strand, within Pf1N1B4_2377 at 2693.412 kb on - strand, within Pf1N1B4_2377 at 2693.733 kb on + strand at 2693.775 kb on + strand at 2693.775 kb on + strand at 2693.801 kb on + strand at 2693.802 kb on - strand at 2693.845 kb on + strand at 2693.845 kb on + strand at 2693.845 kb on + strand at 2693.845 kb on + strand at 2693.845 kb on + strand at 2693.846 kb on - strand at 2693.846 kb on - strand at 2693.852 kb on + strand at 2693.852 kb on + strand at 2693.852 kb on + strand at 2693.853 kb on - strand at 2693.853 kb on - strand at 2693.853 kb on - strand at 2693.854 kb on + strand at 2693.855 kb on - strand at 2693.855 kb on - strand at 2693.896 kb on - strand at 2693.928 kb on + strand at 2693.929 kb on - strand at 2693.949 kb on + strand at 2693.949 kb on + strand at 2693.950 kb on - strand at 2694.042 kb on + strand at 2694.042 kb on + strand at 2694.042 kb on + strand at 2694.217 kb on + strand, within Pf1N1B4_2378 at 2694.217 kb on + strand, within Pf1N1B4_2378 at 2694.217 kb on + strand, within Pf1N1B4_2378 at 2694.218 kb on - strand, within Pf1N1B4_2378 at 2694.218 kb on - strand, within Pf1N1B4_2378 at 2694.218 kb on - strand at 2694.218 kb on - strand, within Pf1N1B4_2378 at 2694.218 kb on - strand, within Pf1N1B4_2378 at 2694.218 kb on - strand, within Pf1N1B4_2378 at 2694.277 kb on + strand, within Pf1N1B4_2378 at 2694.278 kb on - strand, within Pf1N1B4_2378 at 2694.278 kb on - strand, within Pf1N1B4_2378 at 2694.295 kb on + strand, within Pf1N1B4_2378 at 2694.296 kb on - strand, within Pf1N1B4_2378 at 2694.296 kb on - strand, within Pf1N1B4_2378 at 2694.296 kb on - strand, within Pf1N1B4_2378 at 2694.296 kb on - strand, within Pf1N1B4_2378 at 2694.296 kb on - strand, within Pf1N1B4_2378 at 2694.296 kb on - strand, within Pf1N1B4_2378 at 2694.355 kb on + strand, within Pf1N1B4_2378 at 2694.553 kb on + strand, within Pf1N1B4_2378 at 2694.553 kb on + strand, within Pf1N1B4_2378 at 2694.553 kb on + strand, within Pf1N1B4_2378 at 2694.553 kb on + strand, within Pf1N1B4_2378 at 2694.554 kb on - strand, within Pf1N1B4_2378 at 2694.554 kb on - strand, within Pf1N1B4_2378 at 2694.554 kb on - strand, within Pf1N1B4_2378 at 2694.577 kb on + strand, within Pf1N1B4_2378 at 2694.699 kb on + strand, within Pf1N1B4_2378 at 2694.745 kb on + strand, within Pf1N1B4_2378
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 2,691,354 + +1.0 2,691,354 + +0.4 2,691,354 + -0.1 2,691,354 + -0.4 2,691,355 - +2.2 2,691,355 - -0.1 2,692,124 + Pf1N1B4_2376 0.89 -1.2 2,692,124 + Pf1N1B4_2376 0.89 -0.2 2,692,125 - Pf1N1B4_2376 0.89 -1.2 2,692,125 - -0.6 2,692,213 + -1.0 2,692,213 + +1.4 2,692,214 - -0.3 2,692,214 - -0.3 2,692,214 - -1.0 2,692,214 - -0.2 2,692,470 - +0.4 2,692,470 - -1.6 2,692,484 + Pf1N1B4_2377 0.10 -2.6 2,692,485 - Pf1N1B4_2377 0.10 -1.6 2,692,485 - Pf1N1B4_2377 0.10 -0.6 2,692,485 - Pf1N1B4_2377 0.10 -1.6 2,692,781 + Pf1N1B4_2377 0.31 -2.2 2,692,781 + Pf1N1B4_2377 0.31 -2.4 2,692,782 - Pf1N1B4_2377 0.31 -2.6 2,692,826 + Pf1N1B4_2377 0.35 -0.2 2,692,826 + Pf1N1B4_2377 0.35 -2.4 2,692,826 + Pf1N1B4_2377 0.35 -1.2 2,692,839 - Pf1N1B4_2377 0.36 -2.4 2,692,839 - Pf1N1B4_2377 0.36 +0.4 2,692,877 + Pf1N1B4_2377 0.38 -1.2 2,693,036 + Pf1N1B4_2377 0.50 -1.6 2,693,037 - Pf1N1B4_2377 0.50 -2.2 2,693,048 + Pf1N1B4_2377 0.50 +0.4 2,693,048 + Pf1N1B4_2377 0.50 -0.6 2,693,048 + Pf1N1B4_2377 0.50 -1.6 2,693,048 + Pf1N1B4_2377 0.50 -3.2 2,693,048 + Pf1N1B4_2377 0.50 -2.6 2,693,048 + Pf1N1B4_2377 0.50 -1.6 2,693,049 - Pf1N1B4_2377 0.50 -1.2 2,693,192 + Pf1N1B4_2377 0.61 -0.6 2,693,193 - Pf1N1B4_2377 0.61 -2.6 2,693,240 + Pf1N1B4_2377 0.64 +1.4 2,693,240 + Pf1N1B4_2377 0.64 -1.6 2,693,240 + Pf1N1B4_2377 0.64 -0.6 2,693,240 + Pf1N1B4_2377 0.64 +0.4 2,693,241 - Pf1N1B4_2377 0.64 -0.6 2,693,251 - Pf1N1B4_2377 0.65 -0.6 2,693,411 + Pf1N1B4_2377 0.76 -0.6 2,693,411 + Pf1N1B4_2377 0.76 -2.4 2,693,411 + Pf1N1B4_2377 0.76 -1.2 2,693,412 - Pf1N1B4_2377 0.76 -2.4 2,693,412 - Pf1N1B4_2377 0.76 -0.8 2,693,412 - Pf1N1B4_2377 0.76 -2.2 2,693,733 + -1.6 2,693,775 + +0.1 2,693,775 + -1.9 2,693,801 + -1.0 2,693,802 - -0.7 2,693,845 + -0.5 2,693,845 + +1.0 2,693,845 + -0.6 2,693,845 + -0.6 2,693,845 + +1.0 2,693,846 - +2.4 2,693,846 - -1.0 2,693,852 + -1.3 2,693,852 + +1.4 2,693,852 + +0.1 2,693,853 - -1.2 2,693,853 - -0.6 2,693,853 - +0.4 2,693,854 + -0.2 2,693,855 - -0.6 2,693,855 - -1.6 2,693,896 - -0.6 2,693,928 + +0.4 2,693,929 - -1.2 2,693,949 + +1.0 2,693,949 + +0.4 2,693,950 - +0.1 2,694,042 + -1.0 2,694,042 + -0.6 2,694,042 + -0.4 2,694,217 + Pf1N1B4_2378 0.14 +1.4 2,694,217 + Pf1N1B4_2378 0.14 +0.4 2,694,217 + Pf1N1B4_2378 0.14 +0.4 2,694,218 - Pf1N1B4_2378 0.14 +0.1 2,694,218 - Pf1N1B4_2378 0.14 -0.6 2,694,218 - -0.6 2,694,218 - Pf1N1B4_2378 0.14 -0.3 2,694,218 - Pf1N1B4_2378 0.14 -0.6 2,694,218 - Pf1N1B4_2378 0.14 +0.4 2,694,277 + Pf1N1B4_2378 0.17 +1.2 2,694,278 - Pf1N1B4_2378 0.17 -0.2 2,694,278 - Pf1N1B4_2378 0.17 -1.9 2,694,295 + Pf1N1B4_2378 0.18 +0.7 2,694,296 - Pf1N1B4_2378 0.18 -0.6 2,694,296 - Pf1N1B4_2378 0.18 -0.6 2,694,296 - Pf1N1B4_2378 0.18 +0.7 2,694,296 - Pf1N1B4_2378 0.18 -0.6 2,694,296 - Pf1N1B4_2378 0.18 +0.7 2,694,296 - Pf1N1B4_2378 0.18 -1.2 2,694,355 + Pf1N1B4_2378 0.22 -0.2 2,694,553 + Pf1N1B4_2378 0.33 -1.2 2,694,553 + Pf1N1B4_2378 0.33 +2.4 2,694,553 + Pf1N1B4_2378 0.33 -0.6 2,694,553 + Pf1N1B4_2378 0.33 +0.5 2,694,554 - Pf1N1B4_2378 0.33 +0.4 2,694,554 - Pf1N1B4_2378 0.33 -0.0 2,694,554 - Pf1N1B4_2378 0.33 +0.4 2,694,577 + Pf1N1B4_2378 0.34 +0.8 2,694,699 + Pf1N1B4_2378 0.41 -1.6 2,694,745 + Pf1N1B4_2378 0.44 +1.0
Or see this region's nucleotide sequence