Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2354 and Pf1N1B4_2355 are separated by 10 nucleotides Pf1N1B4_2355 and Pf1N1B4_2356 overlap by 20 nucleotides Pf1N1B4_2356 and Pf1N1B4_2357 are separated by 27 nucleotides Pf1N1B4_2357 and Pf1N1B4_2358 are separated by 36 nucleotides
Pf1N1B4_2354 - twitching motility protein PilH, at 2,667,917 to 2,668,282
_2354
Pf1N1B4_2355 - type IV pili signal transduction protein PilI, at 2,668,293 to 2,668,826
_2355
Pf1N1B4_2356 - hypothetical protein, at 2,668,807 to 2,668,929
_2356
Pf1N1B4_2357 - type IV pilus biogenesis protein PilJ, at 2,668,957 to 2,671,014
_2357
Pf1N1B4_2358 - Signal transduction histidine kinase CheA (EC 2.7.3.-), at 2,671,051 to 2,676,954
_2358
Position (kb)
2668
2669
2670
2671
2672 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2667.959 kb on + strand, within Pf1N1B4_2354 at 2667.959 kb on + strand, within Pf1N1B4_2354 at 2667.959 kb on + strand, within Pf1N1B4_2354 at 2667.959 kb on + strand, within Pf1N1B4_2354 at 2667.959 kb on + strand, within Pf1N1B4_2354 at 2667.960 kb on - strand, within Pf1N1B4_2354 at 2667.960 kb on - strand, within Pf1N1B4_2354 at 2667.960 kb on - strand, within Pf1N1B4_2354 at 2668.160 kb on - strand, within Pf1N1B4_2354 at 2668.217 kb on + strand, within Pf1N1B4_2354 at 2668.217 kb on + strand, within Pf1N1B4_2354 at 2668.217 kb on + strand, within Pf1N1B4_2354 at 2668.217 kb on + strand, within Pf1N1B4_2354 at 2668.218 kb on - strand, within Pf1N1B4_2354 at 2668.218 kb on - strand, within Pf1N1B4_2354 at 2668.218 kb on - strand, within Pf1N1B4_2354 at 2668.218 kb on - strand, within Pf1N1B4_2354 at 2668.218 kb on - strand, within Pf1N1B4_2354 at 2668.432 kb on - strand, within Pf1N1B4_2355 at 2668.432 kb on - strand, within Pf1N1B4_2355 at 2668.432 kb on - strand, within Pf1N1B4_2355 at 2668.702 kb on + strand, within Pf1N1B4_2355 at 2668.702 kb on + strand, within Pf1N1B4_2355 at 2668.703 kb on - strand, within Pf1N1B4_2355 at 2668.703 kb on - strand, within Pf1N1B4_2355 at 2668.703 kb on - strand, within Pf1N1B4_2355 at 2668.706 kb on + strand, within Pf1N1B4_2355 at 2668.706 kb on + strand, within Pf1N1B4_2355 at 2668.707 kb on - strand, within Pf1N1B4_2355 at 2668.771 kb on + strand, within Pf1N1B4_2355 at 2668.772 kb on - strand, within Pf1N1B4_2355 at 2668.823 kb on - strand at 2668.823 kb on - strand at 2668.823 kb on - strand at 2668.863 kb on + strand, within Pf1N1B4_2356 at 2668.863 kb on + strand, within Pf1N1B4_2356 at 2668.863 kb on + strand, within Pf1N1B4_2356 at 2668.863 kb on + strand, within Pf1N1B4_2356 at 2668.863 kb on + strand, within Pf1N1B4_2356 at 2668.863 kb on + strand, within Pf1N1B4_2356 at 2668.863 kb on + strand, within Pf1N1B4_2356 at 2668.863 kb on + strand, within Pf1N1B4_2356 at 2668.863 kb on + strand, within Pf1N1B4_2356 at 2668.863 kb on + strand, within Pf1N1B4_2356 at 2668.864 kb on - strand, within Pf1N1B4_2356 at 2668.864 kb on - strand, within Pf1N1B4_2356 at 2668.864 kb on - strand, within Pf1N1B4_2356 at 2668.864 kb on - strand, within Pf1N1B4_2356 at 2668.961 kb on + strand at 2668.961 kb on + strand at 2668.962 kb on - strand at 2668.968 kb on + strand at 2669.068 kb on + strand at 2669.069 kb on - strand at 2669.101 kb on + strand at 2669.102 kb on - strand at 2669.102 kb on - strand at 2669.102 kb on - strand at 2669.102 kb on - strand at 2669.102 kb on - strand at 2669.102 kb on - strand at 2669.102 kb on - strand at 2669.237 kb on - strand, within Pf1N1B4_2357 at 2669.435 kb on - strand, within Pf1N1B4_2357 at 2669.542 kb on - strand, within Pf1N1B4_2357 at 2669.542 kb on - strand, within Pf1N1B4_2357 at 2669.794 kb on - strand, within Pf1N1B4_2357 at 2669.800 kb on - strand, within Pf1N1B4_2357 at 2669.884 kb on - strand, within Pf1N1B4_2357 at 2669.893 kb on + strand, within Pf1N1B4_2357 at 2669.894 kb on - strand, within Pf1N1B4_2357 at 2669.894 kb on - strand, within Pf1N1B4_2357 at 2669.894 kb on - strand, within Pf1N1B4_2357 at 2669.897 kb on + strand, within Pf1N1B4_2357 at 2669.897 kb on + strand, within Pf1N1B4_2357 at 2669.897 kb on + strand, within Pf1N1B4_2357 at 2669.897 kb on + strand, within Pf1N1B4_2357 at 2669.897 kb on + strand, within Pf1N1B4_2357 at 2669.897 kb on + strand, within Pf1N1B4_2357 at 2669.898 kb on - strand, within Pf1N1B4_2357 at 2669.898 kb on - strand, within Pf1N1B4_2357 at 2669.898 kb on - strand, within Pf1N1B4_2357 at 2669.898 kb on - strand, within Pf1N1B4_2357 at 2669.898 kb on - strand, within Pf1N1B4_2357 at 2669.898 kb on - strand, within Pf1N1B4_2357 at 2669.898 kb on - strand, within Pf1N1B4_2357 at 2670.115 kb on + strand, within Pf1N1B4_2357 at 2670.115 kb on + strand, within Pf1N1B4_2357 at 2670.115 kb on + strand, within Pf1N1B4_2357 at 2670.115 kb on + strand, within Pf1N1B4_2357 at 2670.115 kb on + strand, within Pf1N1B4_2357 at 2670.116 kb on - strand, within Pf1N1B4_2357 at 2670.116 kb on - strand, within Pf1N1B4_2357 at 2670.116 kb on - strand, within Pf1N1B4_2357 at 2670.116 kb on - strand, within Pf1N1B4_2357 at 2670.116 kb on - strand, within Pf1N1B4_2357 at 2670.593 kb on + strand, within Pf1N1B4_2357 at 2670.593 kb on + strand, within Pf1N1B4_2357 at 2670.594 kb on - strand at 2670.594 kb on - strand, within Pf1N1B4_2357 at 2670.594 kb on - strand, within Pf1N1B4_2357 at 2670.594 kb on - strand, within Pf1N1B4_2357 at 2670.801 kb on + strand, within Pf1N1B4_2357 at 2670.802 kb on - strand, within Pf1N1B4_2357 at 2670.802 kb on - strand, within Pf1N1B4_2357 at 2670.802 kb on - strand, within Pf1N1B4_2357 at 2670.802 kb on - strand, within Pf1N1B4_2357 at 2670.988 kb on - strand at 2671.029 kb on + strand at 2671.029 kb on + strand at 2671.030 kb on - strand at 2671.046 kb on + strand at 2671.046 kb on + strand at 2671.046 kb on + strand at 2671.047 kb on - strand at 2671.047 kb on - strand at 2671.047 kb on - strand at 2671.047 kb on - strand at 2671.047 kb on - strand at 2671.047 kb on - strand at 2671.051 kb on - strand at 2671.069 kb on + strand at 2671.069 kb on + strand at 2671.069 kb on + strand at 2671.069 kb on + strand at 2671.070 kb on - strand at 2671.070 kb on - strand at 2671.070 kb on - strand at 2671.070 kb on - strand at 2671.070 kb on - strand at 2671.070 kb on - strand at 2671.070 kb on - strand at 2671.070 kb on - strand at 2671.070 kb on - strand at 2671.070 kb on - strand at 2671.070 kb on - strand at 2671.070 kb on - strand at 2671.281 kb on - strand at 2671.281 kb on - strand at 2671.325 kb on + strand at 2671.354 kb on + strand at 2671.355 kb on - strand at 2671.355 kb on - strand at 2671.355 kb on - strand at 2671.389 kb on - strand at 2671.422 kb on - strand at 2671.422 kb on - strand at 2671.491 kb on - strand at 2671.861 kb on + strand, within Pf1N1B4_2358 at 2671.861 kb on + strand, within Pf1N1B4_2358 at 2671.861 kb on + strand, within Pf1N1B4_2358 at 2671.861 kb on + strand, within Pf1N1B4_2358 at 2671.861 kb on + strand, within Pf1N1B4_2358 at 2671.861 kb on + strand, within Pf1N1B4_2358 at 2671.861 kb on + strand, within Pf1N1B4_2358 at 2671.862 kb on - strand, within Pf1N1B4_2358 at 2671.862 kb on - strand, within Pf1N1B4_2358 at 2671.862 kb on - strand, within Pf1N1B4_2358 at 2671.862 kb on - strand, within Pf1N1B4_2358 at 2671.862 kb on - strand, within Pf1N1B4_2358 at 2671.862 kb on - strand, within Pf1N1B4_2358 at 2671.862 kb on - strand, within Pf1N1B4_2358 at 2671.862 kb on - strand, within Pf1N1B4_2358 at 2671.862 kb on - strand, within Pf1N1B4_2358 at 2671.862 kb on - strand, within Pf1N1B4_2358 at 2671.862 kb on - strand, within Pf1N1B4_2358 at 2671.862 kb on - strand, within Pf1N1B4_2358 at 2671.862 kb on - strand, within Pf1N1B4_2358 at 2671.862 kb on - strand, within Pf1N1B4_2358 at 2671.862 kb on - strand, within Pf1N1B4_2358 at 2671.863 kb on + strand, within Pf1N1B4_2358 at 2671.863 kb on + strand, within Pf1N1B4_2358 at 2671.864 kb on - strand, within Pf1N1B4_2358 at 2671.864 kb on - strand, within Pf1N1B4_2358 at 2671.864 kb on - strand, within Pf1N1B4_2358 at 2671.864 kb on - strand, within Pf1N1B4_2358 at 2671.864 kb on - strand, within Pf1N1B4_2358 at 2671.864 kb on - strand, within Pf1N1B4_2358 at 2671.916 kb on - strand, within Pf1N1B4_2358
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 2,667,959 + Pf1N1B4_2354 0.11 -0.1 2,667,959 + Pf1N1B4_2354 0.11 -0.6 2,667,959 + Pf1N1B4_2354 0.11 -1.2 2,667,959 + Pf1N1B4_2354 0.11 -1.2 2,667,959 + Pf1N1B4_2354 0.11 -0.2 2,667,960 - Pf1N1B4_2354 0.12 -0.9 2,667,960 - Pf1N1B4_2354 0.12 -0.0 2,667,960 - Pf1N1B4_2354 0.12 +0.4 2,668,160 - Pf1N1B4_2354 0.66 -0.6 2,668,217 + Pf1N1B4_2354 0.82 -1.7 2,668,217 + Pf1N1B4_2354 0.82 -0.6 2,668,217 + Pf1N1B4_2354 0.82 -2.8 2,668,217 + Pf1N1B4_2354 0.82 -0.6 2,668,218 - Pf1N1B4_2354 0.82 -0.8 2,668,218 - Pf1N1B4_2354 0.82 -1.5 2,668,218 - Pf1N1B4_2354 0.82 -2.2 2,668,218 - Pf1N1B4_2354 0.82 +0.6 2,668,218 - Pf1N1B4_2354 0.82 -0.2 2,668,432 - Pf1N1B4_2355 0.26 +2.0 2,668,432 - Pf1N1B4_2355 0.26 +3.0 2,668,432 - Pf1N1B4_2355 0.26 +2.4 2,668,702 + Pf1N1B4_2355 0.77 -0.2 2,668,702 + Pf1N1B4_2355 0.77 +0.4 2,668,703 - Pf1N1B4_2355 0.77 +1.7 2,668,703 - Pf1N1B4_2355 0.77 +0.4 2,668,703 - Pf1N1B4_2355 0.77 +1.4 2,668,706 + Pf1N1B4_2355 0.77 -0.8 2,668,706 + Pf1N1B4_2355 0.77 -0.4 2,668,707 - Pf1N1B4_2355 0.78 -0.3 2,668,771 + Pf1N1B4_2355 0.90 -1.2 2,668,772 - Pf1N1B4_2355 0.90 +0.2 2,668,823 - -0.6 2,668,823 - +0.7 2,668,823 - +0.4 2,668,863 + Pf1N1B4_2356 0.46 -1.6 2,668,863 + Pf1N1B4_2356 0.46 -0.0 2,668,863 + Pf1N1B4_2356 0.46 +1.7 2,668,863 + Pf1N1B4_2356 0.46 +1.4 2,668,863 + Pf1N1B4_2356 0.46 -2.2 2,668,863 + Pf1N1B4_2356 0.46 +1.0 2,668,863 + Pf1N1B4_2356 0.46 +1.7 2,668,863 + Pf1N1B4_2356 0.46 +2.0 2,668,863 + Pf1N1B4_2356 0.46 +0.8 2,668,863 + Pf1N1B4_2356 0.46 -0.6 2,668,864 - Pf1N1B4_2356 0.46 +0.4 2,668,864 - Pf1N1B4_2356 0.46 +0.4 2,668,864 - Pf1N1B4_2356 0.46 +2.7 2,668,864 - Pf1N1B4_2356 0.46 +0.2 2,668,961 + +2.1 2,668,961 + +1.0 2,668,962 - +1.6 2,668,968 + +2.0 2,669,068 + +1.2 2,669,069 - +0.6 2,669,101 + -1.5 2,669,102 - -0.0 2,669,102 - +0.4 2,669,102 - +1.4 2,669,102 - -0.8 2,669,102 - +1.0 2,669,102 - +1.4 2,669,102 - -1.6 2,669,237 - Pf1N1B4_2357 0.14 +2.0 2,669,435 - Pf1N1B4_2357 0.23 +0.2 2,669,542 - Pf1N1B4_2357 0.28 +0.7 2,669,542 - Pf1N1B4_2357 0.28 +2.0 2,669,794 - Pf1N1B4_2357 0.41 -0.6 2,669,800 - Pf1N1B4_2357 0.41 +0.8 2,669,884 - Pf1N1B4_2357 0.45 +0.3 2,669,893 + Pf1N1B4_2357 0.45 +1.2 2,669,894 - Pf1N1B4_2357 0.46 +0.4 2,669,894 - Pf1N1B4_2357 0.46 -0.1 2,669,894 - Pf1N1B4_2357 0.46 +2.7 2,669,897 + Pf1N1B4_2357 0.46 +2.6 2,669,897 + Pf1N1B4_2357 0.46 +1.4 2,669,897 + Pf1N1B4_2357 0.46 +1.2 2,669,897 + Pf1N1B4_2357 0.46 +2.1 2,669,897 + Pf1N1B4_2357 0.46 +0.4 2,669,897 + Pf1N1B4_2357 0.46 +1.5 2,669,898 - Pf1N1B4_2357 0.46 -0.6 2,669,898 - Pf1N1B4_2357 0.46 +1.4 2,669,898 - Pf1N1B4_2357 0.46 +0.3 2,669,898 - Pf1N1B4_2357 0.46 +0.4 2,669,898 - Pf1N1B4_2357 0.46 +1.0 2,669,898 - Pf1N1B4_2357 0.46 +3.2 2,669,898 - Pf1N1B4_2357 0.46 -0.0 2,670,115 + Pf1N1B4_2357 0.56 +0.8 2,670,115 + Pf1N1B4_2357 0.56 +0.3 2,670,115 + Pf1N1B4_2357 0.56 +2.0 2,670,115 + Pf1N1B4_2357 0.56 +1.0 2,670,115 + Pf1N1B4_2357 0.56 +0.4 2,670,116 - Pf1N1B4_2357 0.56 -0.6 2,670,116 - Pf1N1B4_2357 0.56 +0.7 2,670,116 - Pf1N1B4_2357 0.56 +1.0 2,670,116 - Pf1N1B4_2357 0.56 -0.0 2,670,116 - Pf1N1B4_2357 0.56 +0.4 2,670,593 + Pf1N1B4_2357 0.79 +0.8 2,670,593 + Pf1N1B4_2357 0.79 -0.5 2,670,594 - +1.4 2,670,594 - Pf1N1B4_2357 0.80 +1.3 2,670,594 - Pf1N1B4_2357 0.80 +0.4 2,670,594 - Pf1N1B4_2357 0.80 +1.4 2,670,801 + Pf1N1B4_2357 0.90 +0.8 2,670,802 - Pf1N1B4_2357 0.90 +0.4 2,670,802 - Pf1N1B4_2357 0.90 +0.2 2,670,802 - Pf1N1B4_2357 0.90 -0.2 2,670,802 - Pf1N1B4_2357 0.90 -1.6 2,670,988 - -1.6 2,671,029 + +1.0 2,671,029 + -0.6 2,671,030 - -0.2 2,671,046 + +0.4 2,671,046 + -0.6 2,671,046 + -1.6 2,671,047 - -0.6 2,671,047 - -0.6 2,671,047 - +0.1 2,671,047 - -1.0 2,671,047 - +0.4 2,671,047 - +1.2 2,671,051 - +0.4 2,671,069 + -0.6 2,671,069 + -0.3 2,671,069 + -0.0 2,671,069 + -0.9 2,671,070 - -0.9 2,671,070 - +0.7 2,671,070 - +0.4 2,671,070 - -0.6 2,671,070 - -1.6 2,671,070 - -1.6 2,671,070 - +0.4 2,671,070 - +0.9 2,671,070 - -0.0 2,671,070 - +2.0 2,671,070 - +3.2 2,671,070 - -2.2 2,671,281 - +0.1 2,671,281 - -1.2 2,671,325 + +0.1 2,671,354 + -0.6 2,671,355 - +0.8 2,671,355 - -0.4 2,671,355 - -1.6 2,671,389 - -1.2 2,671,422 - -0.9 2,671,422 - -0.9 2,671,491 - -1.2 2,671,861 + Pf1N1B4_2358 0.14 -2.2 2,671,861 + Pf1N1B4_2358 0.14 -0.6 2,671,861 + Pf1N1B4_2358 0.14 -0.8 2,671,861 + Pf1N1B4_2358 0.14 +0.4 2,671,861 + Pf1N1B4_2358 0.14 +1.0 2,671,861 + Pf1N1B4_2358 0.14 +0.4 2,671,861 + Pf1N1B4_2358 0.14 +0.4 2,671,862 - Pf1N1B4_2358 0.14 -0.6 2,671,862 - Pf1N1B4_2358 0.14 -0.9 2,671,862 - Pf1N1B4_2358 0.14 +0.4 2,671,862 - Pf1N1B4_2358 0.14 -1.2 2,671,862 - Pf1N1B4_2358 0.14 -1.9 2,671,862 - Pf1N1B4_2358 0.14 -1.9 2,671,862 - Pf1N1B4_2358 0.14 -1.5 2,671,862 - Pf1N1B4_2358 0.14 -0.3 2,671,862 - Pf1N1B4_2358 0.14 +0.7 2,671,862 - Pf1N1B4_2358 0.14 -0.3 2,671,862 - Pf1N1B4_2358 0.14 +1.4 2,671,862 - Pf1N1B4_2358 0.14 -0.6 2,671,862 - Pf1N1B4_2358 0.14 -0.4 2,671,862 - Pf1N1B4_2358 0.14 +1.7 2,671,862 - Pf1N1B4_2358 0.14 +0.1 2,671,863 + Pf1N1B4_2358 0.14 -0.1 2,671,863 + Pf1N1B4_2358 0.14 -2.2 2,671,864 - Pf1N1B4_2358 0.14 +0.1 2,671,864 - Pf1N1B4_2358 0.14 -0.1 2,671,864 - Pf1N1B4_2358 0.14 -0.0 2,671,864 - Pf1N1B4_2358 0.14 +0.8 2,671,864 - Pf1N1B4_2358 0.14 -2.4 2,671,864 - Pf1N1B4_2358 0.14 -0.6 2,671,916 - Pf1N1B4_2358 0.15 -1.9
Or see this region's nucleotide sequence