Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_2356

Experiment: L-Isoleucine (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_2353 and Pf1N1B4_2354 are separated by 57 nucleotidesPf1N1B4_2354 and Pf1N1B4_2355 are separated by 10 nucleotidesPf1N1B4_2355 and Pf1N1B4_2356 overlap by 20 nucleotidesPf1N1B4_2356 and Pf1N1B4_2357 are separated by 27 nucleotides Pf1N1B4_2353 - twitching motility protein PilG, at 2,667,455 to 2,667,859 _2353 Pf1N1B4_2354 - twitching motility protein PilH, at 2,667,917 to 2,668,282 _2354 Pf1N1B4_2355 - type IV pili signal transduction protein PilI, at 2,668,293 to 2,668,826 _2355 Pf1N1B4_2356 - hypothetical protein, at 2,668,807 to 2,668,929 _2356 Pf1N1B4_2357 - type IV pilus biogenesis protein PilJ, at 2,668,957 to 2,671,014 _2357 Position (kb) 2668 2669Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 2667.829 kb on - strandat 2667.857 kb on + strandat 2667.858 kb on - strandat 2667.925 kb on + strandat 2667.925 kb on + strandat 2667.925 kb on + strandat 2667.925 kb on + strandat 2667.926 kb on - strandat 2667.926 kb on - strandat 2667.926 kb on - strandat 2667.926 kb on - strandat 2667.926 kb on - strandat 2667.926 kb on - strandat 2667.926 kb on - strandat 2667.926 kb on - strandat 2667.926 kb on - strandat 2667.926 kb on - strandat 2667.926 kb on - strandat 2667.926 kb on - strandat 2667.959 kb on + strand, within Pf1N1B4_2354at 2667.959 kb on + strand, within Pf1N1B4_2354at 2667.959 kb on + strand, within Pf1N1B4_2354at 2667.959 kb on + strand, within Pf1N1B4_2354at 2667.959 kb on + strand, within Pf1N1B4_2354at 2667.960 kb on - strand, within Pf1N1B4_2354at 2667.960 kb on - strand, within Pf1N1B4_2354at 2667.960 kb on - strand, within Pf1N1B4_2354at 2668.160 kb on - strand, within Pf1N1B4_2354at 2668.217 kb on + strand, within Pf1N1B4_2354at 2668.217 kb on + strand, within Pf1N1B4_2354at 2668.217 kb on + strand, within Pf1N1B4_2354at 2668.217 kb on + strand, within Pf1N1B4_2354at 2668.218 kb on - strand, within Pf1N1B4_2354at 2668.218 kb on - strand, within Pf1N1B4_2354at 2668.218 kb on - strand, within Pf1N1B4_2354at 2668.218 kb on - strand, within Pf1N1B4_2354at 2668.218 kb on - strand, within Pf1N1B4_2354at 2668.432 kb on - strand, within Pf1N1B4_2355at 2668.432 kb on - strand, within Pf1N1B4_2355at 2668.432 kb on - strand, within Pf1N1B4_2355at 2668.702 kb on + strand, within Pf1N1B4_2355at 2668.702 kb on + strand, within Pf1N1B4_2355at 2668.703 kb on - strand, within Pf1N1B4_2355at 2668.703 kb on - strand, within Pf1N1B4_2355at 2668.703 kb on - strand, within Pf1N1B4_2355at 2668.706 kb on + strand, within Pf1N1B4_2355at 2668.706 kb on + strand, within Pf1N1B4_2355at 2668.707 kb on - strand, within Pf1N1B4_2355at 2668.771 kb on + strand, within Pf1N1B4_2355at 2668.772 kb on - strand, within Pf1N1B4_2355at 2668.823 kb on - strandat 2668.823 kb on - strandat 2668.823 kb on - strandat 2668.863 kb on + strand, within Pf1N1B4_2356at 2668.863 kb on + strand, within Pf1N1B4_2356at 2668.863 kb on + strand, within Pf1N1B4_2356at 2668.863 kb on + strand, within Pf1N1B4_2356at 2668.863 kb on + strand, within Pf1N1B4_2356at 2668.863 kb on + strand, within Pf1N1B4_2356at 2668.863 kb on + strand, within Pf1N1B4_2356at 2668.863 kb on + strand, within Pf1N1B4_2356at 2668.863 kb on + strand, within Pf1N1B4_2356at 2668.863 kb on + strand, within Pf1N1B4_2356at 2668.864 kb on - strand, within Pf1N1B4_2356at 2668.864 kb on - strand, within Pf1N1B4_2356at 2668.864 kb on - strand, within Pf1N1B4_2356at 2668.864 kb on - strand, within Pf1N1B4_2356at 2668.961 kb on + strandat 2668.961 kb on + strandat 2668.962 kb on - strandat 2668.968 kb on + strandat 2669.068 kb on + strandat 2669.069 kb on - strandat 2669.101 kb on + strandat 2669.102 kb on - strandat 2669.102 kb on - strandat 2669.102 kb on - strandat 2669.102 kb on - strandat 2669.102 kb on - strandat 2669.102 kb on - strandat 2669.102 kb on - strandat 2669.237 kb on - strand, within Pf1N1B4_2357at 2669.435 kb on - strand, within Pf1N1B4_2357at 2669.542 kb on - strand, within Pf1N1B4_2357at 2669.542 kb on - strand, within Pf1N1B4_2357at 2669.794 kb on - strand, within Pf1N1B4_2357at 2669.800 kb on - strand, within Pf1N1B4_2357at 2669.884 kb on - strand, within Pf1N1B4_2357at 2669.893 kb on + strand, within Pf1N1B4_2357at 2669.894 kb on - strand, within Pf1N1B4_2357at 2669.894 kb on - strand, within Pf1N1B4_2357at 2669.894 kb on - strand, within Pf1N1B4_2357at 2669.897 kb on + strand, within Pf1N1B4_2357at 2669.897 kb on + strand, within Pf1N1B4_2357at 2669.897 kb on + strand, within Pf1N1B4_2357at 2669.897 kb on + strand, within Pf1N1B4_2357at 2669.897 kb on + strand, within Pf1N1B4_2357at 2669.897 kb on + strand, within Pf1N1B4_2357at 2669.898 kb on - strand, within Pf1N1B4_2357at 2669.898 kb on - strand, within Pf1N1B4_2357at 2669.898 kb on - strand, within Pf1N1B4_2357at 2669.898 kb on - strand, within Pf1N1B4_2357at 2669.898 kb on - strand, within Pf1N1B4_2357at 2669.898 kb on - strand, within Pf1N1B4_2357at 2669.898 kb on - strand, within Pf1N1B4_2357

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Isoleucine (C)
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2,667,829 - -0.8
2,667,857 + -1.6
2,667,858 - -0.2
2,667,925 + -1.2
2,667,925 + -0.8
2,667,925 + -1.6
2,667,925 + -1.1
2,667,926 - -2.4
2,667,926 - -1.0
2,667,926 - -0.1
2,667,926 - -0.0
2,667,926 - -1.2
2,667,926 - -0.8
2,667,926 - +0.4
2,667,926 - +1.4
2,667,926 - +1.1
2,667,926 - -0.3
2,667,926 - -0.2
2,667,926 - -0.6
2,667,959 + Pf1N1B4_2354 0.11 -0.1
2,667,959 + Pf1N1B4_2354 0.11 -0.6
2,667,959 + Pf1N1B4_2354 0.11 -1.2
2,667,959 + Pf1N1B4_2354 0.11 -1.2
2,667,959 + Pf1N1B4_2354 0.11 -0.2
2,667,960 - Pf1N1B4_2354 0.12 -0.9
2,667,960 - Pf1N1B4_2354 0.12 -0.0
2,667,960 - Pf1N1B4_2354 0.12 +0.4
2,668,160 - Pf1N1B4_2354 0.66 -0.6
2,668,217 + Pf1N1B4_2354 0.82 -1.7
2,668,217 + Pf1N1B4_2354 0.82 -0.6
2,668,217 + Pf1N1B4_2354 0.82 -2.8
2,668,217 + Pf1N1B4_2354 0.82 -0.6
2,668,218 - Pf1N1B4_2354 0.82 -0.8
2,668,218 - Pf1N1B4_2354 0.82 -1.5
2,668,218 - Pf1N1B4_2354 0.82 -2.2
2,668,218 - Pf1N1B4_2354 0.82 +0.6
2,668,218 - Pf1N1B4_2354 0.82 -0.2
2,668,432 - Pf1N1B4_2355 0.26 +2.0
2,668,432 - Pf1N1B4_2355 0.26 +3.0
2,668,432 - Pf1N1B4_2355 0.26 +2.4
2,668,702 + Pf1N1B4_2355 0.77 -0.2
2,668,702 + Pf1N1B4_2355 0.77 +0.4
2,668,703 - Pf1N1B4_2355 0.77 +1.7
2,668,703 - Pf1N1B4_2355 0.77 +0.4
2,668,703 - Pf1N1B4_2355 0.77 +1.4
2,668,706 + Pf1N1B4_2355 0.77 -0.8
2,668,706 + Pf1N1B4_2355 0.77 -0.4
2,668,707 - Pf1N1B4_2355 0.78 -0.3
2,668,771 + Pf1N1B4_2355 0.90 -1.2
2,668,772 - Pf1N1B4_2355 0.90 +0.2
2,668,823 - -0.6
2,668,823 - +0.7
2,668,823 - +0.4
2,668,863 + Pf1N1B4_2356 0.46 -1.6
2,668,863 + Pf1N1B4_2356 0.46 -0.0
2,668,863 + Pf1N1B4_2356 0.46 +1.7
2,668,863 + Pf1N1B4_2356 0.46 +1.4
2,668,863 + Pf1N1B4_2356 0.46 -2.2
2,668,863 + Pf1N1B4_2356 0.46 +1.0
2,668,863 + Pf1N1B4_2356 0.46 +1.7
2,668,863 + Pf1N1B4_2356 0.46 +2.0
2,668,863 + Pf1N1B4_2356 0.46 +0.8
2,668,863 + Pf1N1B4_2356 0.46 -0.6
2,668,864 - Pf1N1B4_2356 0.46 +0.4
2,668,864 - Pf1N1B4_2356 0.46 +0.4
2,668,864 - Pf1N1B4_2356 0.46 +2.7
2,668,864 - Pf1N1B4_2356 0.46 +0.2
2,668,961 + +2.1
2,668,961 + +1.0
2,668,962 - +1.6
2,668,968 + +2.0
2,669,068 + +1.2
2,669,069 - +0.6
2,669,101 + -1.5
2,669,102 - -0.0
2,669,102 - +0.4
2,669,102 - +1.4
2,669,102 - -0.8
2,669,102 - +1.0
2,669,102 - +1.4
2,669,102 - -1.6
2,669,237 - Pf1N1B4_2357 0.14 +2.0
2,669,435 - Pf1N1B4_2357 0.23 +0.2
2,669,542 - Pf1N1B4_2357 0.28 +0.7
2,669,542 - Pf1N1B4_2357 0.28 +2.0
2,669,794 - Pf1N1B4_2357 0.41 -0.6
2,669,800 - Pf1N1B4_2357 0.41 +0.8
2,669,884 - Pf1N1B4_2357 0.45 +0.3
2,669,893 + Pf1N1B4_2357 0.45 +1.2
2,669,894 - Pf1N1B4_2357 0.46 +0.4
2,669,894 - Pf1N1B4_2357 0.46 -0.1
2,669,894 - Pf1N1B4_2357 0.46 +2.7
2,669,897 + Pf1N1B4_2357 0.46 +2.6
2,669,897 + Pf1N1B4_2357 0.46 +1.4
2,669,897 + Pf1N1B4_2357 0.46 +1.2
2,669,897 + Pf1N1B4_2357 0.46 +2.1
2,669,897 + Pf1N1B4_2357 0.46 +0.4
2,669,897 + Pf1N1B4_2357 0.46 +1.5
2,669,898 - Pf1N1B4_2357 0.46 -0.6
2,669,898 - Pf1N1B4_2357 0.46 +1.4
2,669,898 - Pf1N1B4_2357 0.46 +0.3
2,669,898 - Pf1N1B4_2357 0.46 +0.4
2,669,898 - Pf1N1B4_2357 0.46 +1.0
2,669,898 - Pf1N1B4_2357 0.46 +3.2
2,669,898 - Pf1N1B4_2357 0.46 -0.0

Or see this region's nucleotide sequence