Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2353 and Pf1N1B4_2354 are separated by 57 nucleotides Pf1N1B4_2354 and Pf1N1B4_2355 are separated by 10 nucleotides Pf1N1B4_2355 and Pf1N1B4_2356 overlap by 20 nucleotides Pf1N1B4_2356 and Pf1N1B4_2357 are separated by 27 nucleotides
Pf1N1B4_2353 - twitching motility protein PilG, at 2,667,455 to 2,667,859
_2353
Pf1N1B4_2354 - twitching motility protein PilH, at 2,667,917 to 2,668,282
_2354
Pf1N1B4_2355 - type IV pili signal transduction protein PilI, at 2,668,293 to 2,668,826
_2355
Pf1N1B4_2356 - hypothetical protein, at 2,668,807 to 2,668,929
_2356
Pf1N1B4_2357 - type IV pilus biogenesis protein PilJ, at 2,668,957 to 2,671,014
_2357
Position (kb)
2668
2669 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2667.328 kb on - strand at 2667.379 kb on + strand at 2667.379 kb on + strand at 2667.379 kb on + strand at 2667.380 kb on - strand at 2667.380 kb on - strand at 2667.380 kb on - strand at 2667.383 kb on + strand at 2667.383 kb on + strand at 2667.383 kb on + strand at 2667.383 kb on + strand at 2667.383 kb on + strand at 2667.383 kb on + strand at 2667.384 kb on - strand at 2667.384 kb on - strand at 2667.384 kb on - strand at 2667.384 kb on - strand at 2667.384 kb on - strand at 2667.384 kb on - strand at 2667.384 kb on - strand at 2667.384 kb on - strand at 2667.384 kb on - strand at 2667.384 kb on - strand at 2667.384 kb on - strand at 2667.446 kb on - strand at 2667.446 kb on - strand at 2667.670 kb on - strand, within Pf1N1B4_2353 at 2667.709 kb on + strand, within Pf1N1B4_2353 at 2667.709 kb on + strand, within Pf1N1B4_2353 at 2667.709 kb on + strand, within Pf1N1B4_2353 at 2667.709 kb on + strand, within Pf1N1B4_2353 at 2667.709 kb on + strand, within Pf1N1B4_2353 at 2667.709 kb on + strand, within Pf1N1B4_2353 at 2667.709 kb on + strand, within Pf1N1B4_2353 at 2667.709 kb on + strand, within Pf1N1B4_2353 at 2667.710 kb on - strand, within Pf1N1B4_2353 at 2667.710 kb on - strand, within Pf1N1B4_2353 at 2667.710 kb on - strand, within Pf1N1B4_2353 at 2667.710 kb on - strand, within Pf1N1B4_2353 at 2667.710 kb on - strand, within Pf1N1B4_2353 at 2667.829 kb on - strand at 2667.857 kb on + strand at 2667.858 kb on - strand at 2667.925 kb on + strand at 2667.925 kb on + strand at 2667.925 kb on + strand at 2667.925 kb on + strand at 2667.926 kb on - strand at 2667.926 kb on - strand at 2667.926 kb on - strand at 2667.926 kb on - strand at 2667.926 kb on - strand at 2667.926 kb on - strand at 2667.926 kb on - strand at 2667.926 kb on - strand at 2667.926 kb on - strand at 2667.926 kb on - strand at 2667.926 kb on - strand at 2667.926 kb on - strand at 2667.959 kb on + strand, within Pf1N1B4_2354 at 2667.959 kb on + strand, within Pf1N1B4_2354 at 2667.959 kb on + strand, within Pf1N1B4_2354 at 2667.959 kb on + strand, within Pf1N1B4_2354 at 2667.959 kb on + strand, within Pf1N1B4_2354 at 2667.960 kb on - strand, within Pf1N1B4_2354 at 2667.960 kb on - strand, within Pf1N1B4_2354 at 2667.960 kb on - strand, within Pf1N1B4_2354 at 2668.160 kb on - strand, within Pf1N1B4_2354 at 2668.217 kb on + strand, within Pf1N1B4_2354 at 2668.217 kb on + strand, within Pf1N1B4_2354 at 2668.217 kb on + strand, within Pf1N1B4_2354 at 2668.217 kb on + strand, within Pf1N1B4_2354 at 2668.218 kb on - strand, within Pf1N1B4_2354 at 2668.218 kb on - strand, within Pf1N1B4_2354 at 2668.218 kb on - strand, within Pf1N1B4_2354 at 2668.218 kb on - strand, within Pf1N1B4_2354 at 2668.218 kb on - strand, within Pf1N1B4_2354 at 2668.432 kb on - strand, within Pf1N1B4_2355 at 2668.432 kb on - strand, within Pf1N1B4_2355 at 2668.432 kb on - strand, within Pf1N1B4_2355 at 2668.702 kb on + strand, within Pf1N1B4_2355 at 2668.702 kb on + strand, within Pf1N1B4_2355 at 2668.703 kb on - strand, within Pf1N1B4_2355 at 2668.703 kb on - strand, within Pf1N1B4_2355 at 2668.703 kb on - strand, within Pf1N1B4_2355 at 2668.706 kb on + strand, within Pf1N1B4_2355 at 2668.706 kb on + strand, within Pf1N1B4_2355 at 2668.707 kb on - strand, within Pf1N1B4_2355 at 2668.771 kb on + strand, within Pf1N1B4_2355 at 2668.772 kb on - strand, within Pf1N1B4_2355 at 2668.823 kb on - strand at 2668.823 kb on - strand at 2668.823 kb on - strand at 2668.863 kb on + strand, within Pf1N1B4_2356 at 2668.863 kb on + strand, within Pf1N1B4_2356 at 2668.863 kb on + strand, within Pf1N1B4_2356 at 2668.863 kb on + strand, within Pf1N1B4_2356 at 2668.863 kb on + strand, within Pf1N1B4_2356 at 2668.863 kb on + strand, within Pf1N1B4_2356 at 2668.863 kb on + strand, within Pf1N1B4_2356 at 2668.863 kb on + strand, within Pf1N1B4_2356 at 2668.863 kb on + strand, within Pf1N1B4_2356 at 2668.863 kb on + strand, within Pf1N1B4_2356 at 2668.864 kb on - strand, within Pf1N1B4_2356 at 2668.864 kb on - strand, within Pf1N1B4_2356 at 2668.864 kb on - strand, within Pf1N1B4_2356 at 2668.864 kb on - strand, within Pf1N1B4_2356 at 2668.961 kb on + strand at 2668.961 kb on + strand at 2668.962 kb on - strand at 2668.968 kb on + strand at 2669.068 kb on + strand at 2669.069 kb on - strand at 2669.101 kb on + strand at 2669.102 kb on - strand at 2669.102 kb on - strand at 2669.102 kb on - strand at 2669.102 kb on - strand at 2669.102 kb on - strand at 2669.102 kb on - strand at 2669.102 kb on - strand at 2669.237 kb on - strand, within Pf1N1B4_2357 at 2669.435 kb on - strand, within Pf1N1B4_2357 at 2669.542 kb on - strand, within Pf1N1B4_2357 at 2669.542 kb on - strand, within Pf1N1B4_2357 at 2669.794 kb on - strand, within Pf1N1B4_2357 at 2669.800 kb on - strand, within Pf1N1B4_2357
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 2,667,328 - -0.6 2,667,379 + -0.7 2,667,379 + -0.7 2,667,379 + +0.4 2,667,380 - +0.1 2,667,380 - +1.0 2,667,380 - +1.4 2,667,383 + -1.4 2,667,383 + +0.4 2,667,383 + -0.0 2,667,383 + -1.4 2,667,383 + -0.6 2,667,383 + -0.6 2,667,384 - -0.0 2,667,384 - +1.4 2,667,384 - -0.0 2,667,384 - +1.4 2,667,384 - -1.2 2,667,384 - -0.7 2,667,384 - -2.2 2,667,384 - -2.6 2,667,384 - -0.4 2,667,384 - -1.2 2,667,384 - +0.1 2,667,446 - -0.9 2,667,446 - -1.2 2,667,670 - Pf1N1B4_2353 0.53 -1.8 2,667,709 + Pf1N1B4_2353 0.63 +0.4 2,667,709 + Pf1N1B4_2353 0.63 +0.4 2,667,709 + Pf1N1B4_2353 0.63 -1.9 2,667,709 + Pf1N1B4_2353 0.63 -1.9 2,667,709 + Pf1N1B4_2353 0.63 +0.1 2,667,709 + Pf1N1B4_2353 0.63 -1.6 2,667,709 + Pf1N1B4_2353 0.63 -0.6 2,667,709 + Pf1N1B4_2353 0.63 -0.2 2,667,710 - Pf1N1B4_2353 0.63 -1.2 2,667,710 - Pf1N1B4_2353 0.63 -0.8 2,667,710 - Pf1N1B4_2353 0.63 -1.0 2,667,710 - Pf1N1B4_2353 0.63 -0.2 2,667,710 - Pf1N1B4_2353 0.63 -0.0 2,667,829 - -0.8 2,667,857 + -1.6 2,667,858 - -0.2 2,667,925 + -1.2 2,667,925 + -0.8 2,667,925 + -1.6 2,667,925 + -1.1 2,667,926 - -2.4 2,667,926 - -1.0 2,667,926 - -0.1 2,667,926 - -0.0 2,667,926 - -1.2 2,667,926 - -0.8 2,667,926 - +0.4 2,667,926 - +1.4 2,667,926 - +1.1 2,667,926 - -0.3 2,667,926 - -0.2 2,667,926 - -0.6 2,667,959 + Pf1N1B4_2354 0.11 -0.1 2,667,959 + Pf1N1B4_2354 0.11 -0.6 2,667,959 + Pf1N1B4_2354 0.11 -1.2 2,667,959 + Pf1N1B4_2354 0.11 -1.2 2,667,959 + Pf1N1B4_2354 0.11 -0.2 2,667,960 - Pf1N1B4_2354 0.12 -0.9 2,667,960 - Pf1N1B4_2354 0.12 -0.0 2,667,960 - Pf1N1B4_2354 0.12 +0.4 2,668,160 - Pf1N1B4_2354 0.66 -0.6 2,668,217 + Pf1N1B4_2354 0.82 -1.7 2,668,217 + Pf1N1B4_2354 0.82 -0.6 2,668,217 + Pf1N1B4_2354 0.82 -2.8 2,668,217 + Pf1N1B4_2354 0.82 -0.6 2,668,218 - Pf1N1B4_2354 0.82 -0.8 2,668,218 - Pf1N1B4_2354 0.82 -1.5 2,668,218 - Pf1N1B4_2354 0.82 -2.2 2,668,218 - Pf1N1B4_2354 0.82 +0.6 2,668,218 - Pf1N1B4_2354 0.82 -0.2 2,668,432 - Pf1N1B4_2355 0.26 +2.0 2,668,432 - Pf1N1B4_2355 0.26 +3.0 2,668,432 - Pf1N1B4_2355 0.26 +2.4 2,668,702 + Pf1N1B4_2355 0.77 -0.2 2,668,702 + Pf1N1B4_2355 0.77 +0.4 2,668,703 - Pf1N1B4_2355 0.77 +1.7 2,668,703 - Pf1N1B4_2355 0.77 +0.4 2,668,703 - Pf1N1B4_2355 0.77 +1.4 2,668,706 + Pf1N1B4_2355 0.77 -0.8 2,668,706 + Pf1N1B4_2355 0.77 -0.4 2,668,707 - Pf1N1B4_2355 0.78 -0.3 2,668,771 + Pf1N1B4_2355 0.90 -1.2 2,668,772 - Pf1N1B4_2355 0.90 +0.2 2,668,823 - -0.6 2,668,823 - +0.7 2,668,823 - +0.4 2,668,863 + Pf1N1B4_2356 0.46 -1.6 2,668,863 + Pf1N1B4_2356 0.46 -0.0 2,668,863 + Pf1N1B4_2356 0.46 +1.7 2,668,863 + Pf1N1B4_2356 0.46 +1.4 2,668,863 + Pf1N1B4_2356 0.46 -2.2 2,668,863 + Pf1N1B4_2356 0.46 +1.0 2,668,863 + Pf1N1B4_2356 0.46 +1.7 2,668,863 + Pf1N1B4_2356 0.46 +2.0 2,668,863 + Pf1N1B4_2356 0.46 +0.8 2,668,863 + Pf1N1B4_2356 0.46 -0.6 2,668,864 - Pf1N1B4_2356 0.46 +0.4 2,668,864 - Pf1N1B4_2356 0.46 +0.4 2,668,864 - Pf1N1B4_2356 0.46 +2.7 2,668,864 - Pf1N1B4_2356 0.46 +0.2 2,668,961 + +2.1 2,668,961 + +1.0 2,668,962 - +1.6 2,668,968 + +2.0 2,669,068 + +1.2 2,669,069 - +0.6 2,669,101 + -1.5 2,669,102 - -0.0 2,669,102 - +0.4 2,669,102 - +1.4 2,669,102 - -0.8 2,669,102 - +1.0 2,669,102 - +1.4 2,669,102 - -1.6 2,669,237 - Pf1N1B4_2357 0.14 +2.0 2,669,435 - Pf1N1B4_2357 0.23 +0.2 2,669,542 - Pf1N1B4_2357 0.28 +0.7 2,669,542 - Pf1N1B4_2357 0.28 +2.0 2,669,794 - Pf1N1B4_2357 0.41 -0.6 2,669,800 - Pf1N1B4_2357 0.41 +0.8
Or see this region's nucleotide sequence