Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2335 and Pf1N1B4_2336 overlap by 4 nucleotides Pf1N1B4_2336 and Pf1N1B4_2337 are separated by 26 nucleotides Pf1N1B4_2337 and Pf1N1B4_2338 are separated by 7 nucleotides
Pf1N1B4_2335 - Nucleoside 5-triphosphatase RdgB (dHAPTP, dITP, XTP-specific) (EC 3.6.1.15), at 2,653,048 to 2,653,644
_2335
Pf1N1B4_2336 - FIG003551: hypothetical protein, at 2,653,641 to 2,654,075
_2336
Pf1N1B4_2337 - Homoserine O-acetyltransferase (EC 2.3.1.31), at 2,654,102 to 2,654,722
_2337
Pf1N1B4_2338 - Homoserine O-acetyltransferase (EC 2.3.1.31), at 2,654,730 to 2,655,869
_2338
Position (kb)
2654
2655 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1
2 at 2653.125 kb on + strand, within Pf1N1B4_2335 at 2653.125 kb on + strand, within Pf1N1B4_2335 at 2653.126 kb on - strand, within Pf1N1B4_2335 at 2653.126 kb on - strand, within Pf1N1B4_2335 at 2653.126 kb on - strand, within Pf1N1B4_2335 at 2653.126 kb on - strand, within Pf1N1B4_2335 at 2653.389 kb on - strand, within Pf1N1B4_2335 at 2653.389 kb on - strand, within Pf1N1B4_2335 at 2653.389 kb on - strand, within Pf1N1B4_2335 at 2653.756 kb on + strand, within Pf1N1B4_2336 at 2653.756 kb on + strand, within Pf1N1B4_2336 at 2653.756 kb on + strand, within Pf1N1B4_2336 at 2653.756 kb on + strand at 2653.756 kb on + strand, within Pf1N1B4_2336 at 2653.756 kb on + strand, within Pf1N1B4_2336 at 2653.756 kb on + strand, within Pf1N1B4_2336 at 2653.757 kb on - strand, within Pf1N1B4_2336 at 2653.757 kb on - strand, within Pf1N1B4_2336 at 2653.759 kb on + strand, within Pf1N1B4_2336 at 2653.856 kb on + strand at 2653.857 kb on - strand, within Pf1N1B4_2336 at 2653.963 kb on + strand, within Pf1N1B4_2336 at 2653.963 kb on + strand, within Pf1N1B4_2336 at 2653.963 kb on + strand, within Pf1N1B4_2336 at 2653.963 kb on + strand, within Pf1N1B4_2336 at 2653.963 kb on + strand, within Pf1N1B4_2336 at 2653.963 kb on + strand, within Pf1N1B4_2336 at 2653.964 kb on - strand, within Pf1N1B4_2336 at 2653.964 kb on - strand, within Pf1N1B4_2336 at 2654.048 kb on + strand at 2654.049 kb on - strand at 2654.049 kb on - strand at 2654.052 kb on + strand at 2654.052 kb on + strand at 2654.053 kb on - strand at 2654.064 kb on + strand at 2654.064 kb on + strand at 2654.165 kb on + strand, within Pf1N1B4_2337 at 2654.174 kb on - strand, within Pf1N1B4_2337 at 2654.180 kb on + strand, within Pf1N1B4_2337 at 2654.180 kb on + strand, within Pf1N1B4_2337 at 2654.180 kb on + strand, within Pf1N1B4_2337 at 2654.180 kb on + strand, within Pf1N1B4_2337 at 2654.181 kb on - strand, within Pf1N1B4_2337 at 2654.181 kb on - strand, within Pf1N1B4_2337 at 2654.307 kb on + strand, within Pf1N1B4_2337 at 2654.307 kb on + strand, within Pf1N1B4_2337 at 2654.307 kb on + strand, within Pf1N1B4_2337 at 2654.308 kb on - strand, within Pf1N1B4_2337 at 2654.316 kb on + strand, within Pf1N1B4_2337 at 2654.316 kb on + strand, within Pf1N1B4_2337 at 2654.316 kb on + strand, within Pf1N1B4_2337 at 2654.316 kb on + strand, within Pf1N1B4_2337 at 2654.316 kb on + strand, within Pf1N1B4_2337 at 2654.316 kb on + strand, within Pf1N1B4_2337 at 2654.316 kb on + strand at 2654.317 kb on - strand, within Pf1N1B4_2337 at 2654.317 kb on - strand, within Pf1N1B4_2337 at 2654.451 kb on + strand, within Pf1N1B4_2337 at 2654.739 kb on + strand at 2654.739 kb on + strand at 2654.741 kb on + strand at 2654.741 kb on + strand at 2654.742 kb on - strand at 2654.752 kb on + strand at 2654.752 kb on + strand at 2654.752 kb on + strand at 2654.752 kb on + strand at 2654.752 kb on + strand at 2654.752 kb on + strand at 2654.752 kb on + strand at 2654.752 kb on + strand at 2654.752 kb on + strand at 2654.753 kb on - strand at 2654.753 kb on - strand at 2654.773 kb on + strand at 2654.773 kb on + strand at 2654.773 kb on + strand at 2654.773 kb on + strand at 2654.993 kb on - strand, within Pf1N1B4_2338 at 2654.993 kb on - strand, within Pf1N1B4_2338 at 2654.993 kb on - strand, within Pf1N1B4_2338 at 2655.019 kb on + strand, within Pf1N1B4_2338 at 2655.019 kb on + strand, within Pf1N1B4_2338 at 2655.019 kb on + strand, within Pf1N1B4_2338 at 2655.019 kb on + strand, within Pf1N1B4_2338 at 2655.019 kb on + strand, within Pf1N1B4_2338 at 2655.020 kb on - strand, within Pf1N1B4_2338 at 2655.020 kb on - strand, within Pf1N1B4_2338 at 2655.062 kb on - strand, within Pf1N1B4_2338 at 2655.070 kb on + strand, within Pf1N1B4_2338 at 2655.070 kb on + strand, within Pf1N1B4_2338 at 2655.070 kb on + strand, within Pf1N1B4_2338 at 2655.070 kb on + strand, within Pf1N1B4_2338 at 2655.071 kb on - strand, within Pf1N1B4_2338 at 2655.071 kb on - strand, within Pf1N1B4_2338 at 2655.106 kb on + strand, within Pf1N1B4_2338 at 2655.106 kb on + strand, within Pf1N1B4_2338 at 2655.107 kb on - strand, within Pf1N1B4_2338 at 2655.107 kb on - strand, within Pf1N1B4_2338 at 2655.169 kb on + strand, within Pf1N1B4_2338 at 2655.169 kb on + strand, within Pf1N1B4_2338 at 2655.169 kb on + strand, within Pf1N1B4_2338 at 2655.169 kb on + strand, within Pf1N1B4_2338 at 2655.169 kb on + strand, within Pf1N1B4_2338 at 2655.169 kb on + strand, within Pf1N1B4_2338 at 2655.169 kb on + strand, within Pf1N1B4_2338 at 2655.169 kb on + strand, within Pf1N1B4_2338 at 2655.169 kb on + strand, within Pf1N1B4_2338 at 2655.170 kb on - strand, within Pf1N1B4_2338 at 2655.170 kb on - strand, within Pf1N1B4_2338 at 2655.170 kb on - strand, within Pf1N1B4_2338 at 2655.170 kb on - strand, within Pf1N1B4_2338 at 2655.569 kb on + strand, within Pf1N1B4_2338 at 2655.569 kb on + strand at 2655.569 kb on + strand, within Pf1N1B4_2338 at 2655.570 kb on - strand, within Pf1N1B4_2338
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 2,653,125 + Pf1N1B4_2335 0.13 +0.4 2,653,125 + Pf1N1B4_2335 0.13 +0.3 2,653,126 - Pf1N1B4_2335 0.13 -1.2 2,653,126 - Pf1N1B4_2335 0.13 -1.2 2,653,126 - Pf1N1B4_2335 0.13 +0.8 2,653,126 - Pf1N1B4_2335 0.13 -1.9 2,653,389 - Pf1N1B4_2335 0.57 -0.6 2,653,389 - Pf1N1B4_2335 0.57 -0.3 2,653,389 - Pf1N1B4_2335 0.57 -1.2 2,653,756 + Pf1N1B4_2336 0.26 -2.6 2,653,756 + Pf1N1B4_2336 0.26 +1.4 2,653,756 + Pf1N1B4_2336 0.26 +0.1 2,653,756 + +0.4 2,653,756 + Pf1N1B4_2336 0.26 +0.8 2,653,756 + Pf1N1B4_2336 0.26 -0.3 2,653,756 + Pf1N1B4_2336 0.26 +0.4 2,653,757 - Pf1N1B4_2336 0.27 +0.4 2,653,757 - Pf1N1B4_2336 0.27 -1.9 2,653,759 + Pf1N1B4_2336 0.27 +0.4 2,653,856 + +2.7 2,653,857 - Pf1N1B4_2336 0.50 +0.7 2,653,963 + Pf1N1B4_2336 0.74 -0.6 2,653,963 + Pf1N1B4_2336 0.74 +0.4 2,653,963 + Pf1N1B4_2336 0.74 -0.1 2,653,963 + Pf1N1B4_2336 0.74 +0.9 2,653,963 + Pf1N1B4_2336 0.74 -0.3 2,653,963 + Pf1N1B4_2336 0.74 -0.6 2,653,964 - Pf1N1B4_2336 0.74 +0.5 2,653,964 - Pf1N1B4_2336 0.74 -0.6 2,654,048 + -2.2 2,654,049 - -0.0 2,654,049 - +0.4 2,654,052 + -0.6 2,654,052 + +1.0 2,654,053 - -1.9 2,654,064 + -1.4 2,654,064 + -0.3 2,654,165 + Pf1N1B4_2337 0.10 -1.6 2,654,174 - Pf1N1B4_2337 0.12 -1.4 2,654,180 + Pf1N1B4_2337 0.13 -3.3 2,654,180 + Pf1N1B4_2337 0.13 -0.6 2,654,180 + Pf1N1B4_2337 0.13 -1.9 2,654,180 + Pf1N1B4_2337 0.13 -1.2 2,654,181 - Pf1N1B4_2337 0.13 -4.8 2,654,181 - Pf1N1B4_2337 0.13 -1.6 2,654,307 + Pf1N1B4_2337 0.33 -2.1 2,654,307 + Pf1N1B4_2337 0.33 +0.4 2,654,307 + Pf1N1B4_2337 0.33 -1.9 2,654,308 - Pf1N1B4_2337 0.33 -1.2 2,654,316 + Pf1N1B4_2337 0.34 -0.6 2,654,316 + Pf1N1B4_2337 0.34 +0.4 2,654,316 + Pf1N1B4_2337 0.34 +0.4 2,654,316 + Pf1N1B4_2337 0.34 -1.6 2,654,316 + Pf1N1B4_2337 0.34 -1.2 2,654,316 + Pf1N1B4_2337 0.34 -3.6 2,654,316 + -0.6 2,654,317 - Pf1N1B4_2337 0.35 -0.6 2,654,317 - Pf1N1B4_2337 0.35 -0.8 2,654,451 + Pf1N1B4_2337 0.56 -1.2 2,654,739 + -1.6 2,654,739 + -2.6 2,654,741 + +1.1 2,654,741 + -3.2 2,654,742 - -0.6 2,654,752 + +0.4 2,654,752 + -1.2 2,654,752 + -3.7 2,654,752 + -2.4 2,654,752 + -1.6 2,654,752 + -5.1 2,654,752 + -1.2 2,654,752 + -1.2 2,654,752 + -4.4 2,654,753 - -0.6 2,654,753 - -2.8 2,654,773 + -1.6 2,654,773 + -2.6 2,654,773 + -1.2 2,654,773 + -1.6 2,654,993 - Pf1N1B4_2338 0.23 -1.6 2,654,993 - Pf1N1B4_2338 0.23 -1.9 2,654,993 - Pf1N1B4_2338 0.23 -1.2 2,655,019 + Pf1N1B4_2338 0.25 -1.2 2,655,019 + Pf1N1B4_2338 0.25 -0.6 2,655,019 + Pf1N1B4_2338 0.25 -2.4 2,655,019 + Pf1N1B4_2338 0.25 +0.4 2,655,019 + Pf1N1B4_2338 0.25 -1.6 2,655,020 - Pf1N1B4_2338 0.25 -2.4 2,655,020 - Pf1N1B4_2338 0.25 -1.9 2,655,062 - Pf1N1B4_2338 0.29 -2.4 2,655,070 + Pf1N1B4_2338 0.30 -1.6 2,655,070 + Pf1N1B4_2338 0.30 -1.9 2,655,070 + Pf1N1B4_2338 0.30 -0.0 2,655,070 + Pf1N1B4_2338 0.30 -1.2 2,655,071 - Pf1N1B4_2338 0.30 -2.9 2,655,071 - Pf1N1B4_2338 0.30 -1.2 2,655,106 + Pf1N1B4_2338 0.33 -0.6 2,655,106 + Pf1N1B4_2338 0.33 -6.0 2,655,107 - Pf1N1B4_2338 0.33 -1.2 2,655,107 - Pf1N1B4_2338 0.33 -2.4 2,655,169 + Pf1N1B4_2338 0.39 -1.6 2,655,169 + Pf1N1B4_2338 0.39 -0.2 2,655,169 + Pf1N1B4_2338 0.39 -0.6 2,655,169 + Pf1N1B4_2338 0.39 -1.6 2,655,169 + Pf1N1B4_2338 0.39 -2.8 2,655,169 + Pf1N1B4_2338 0.39 -3.9 2,655,169 + Pf1N1B4_2338 0.39 -1.6 2,655,169 + Pf1N1B4_2338 0.39 -1.9 2,655,169 + Pf1N1B4_2338 0.39 -1.2 2,655,170 - Pf1N1B4_2338 0.39 -0.6 2,655,170 - Pf1N1B4_2338 0.39 -1.8 2,655,170 - Pf1N1B4_2338 0.39 +0.4 2,655,170 - Pf1N1B4_2338 0.39 +0.4 2,655,569 + Pf1N1B4_2338 0.74 -1.2 2,655,569 + -1.6 2,655,569 + Pf1N1B4_2338 0.74 +0.4 2,655,570 - Pf1N1B4_2338 0.74 -4.6
Or see this region's nucleotide sequence