Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2294 and Pf1N1B4_2295 are separated by 39 nucleotides Pf1N1B4_2295 and Pf1N1B4_2296 are separated by 124 nucleotides
Pf1N1B4_2294 - Ferric iron ABC transporter, permease protein, at 2,610,965 to 2,612,689
_2294
Pf1N1B4_2295 - Ferric iron ABC transporter, iron-binding protein, at 2,612,729 to 2,613,754
_2295
Pf1N1B4_2296 - FIG00350678: hypothetical protein, at 2,613,879 to 2,615,012
_2296
Position (kb)
2612
2613
2614 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2611.883 kb on + strand, within Pf1N1B4_2294 at 2611.884 kb on - strand, within Pf1N1B4_2294 at 2611.884 kb on - strand, within Pf1N1B4_2294 at 2611.970 kb on + strand, within Pf1N1B4_2294 at 2612.036 kb on + strand at 2612.065 kb on + strand, within Pf1N1B4_2294 at 2612.065 kb on + strand, within Pf1N1B4_2294 at 2612.066 kb on - strand, within Pf1N1B4_2294 at 2612.066 kb on - strand, within Pf1N1B4_2294 at 2612.105 kb on + strand, within Pf1N1B4_2294 at 2612.105 kb on + strand, within Pf1N1B4_2294 at 2612.106 kb on - strand, within Pf1N1B4_2294 at 2612.106 kb on - strand, within Pf1N1B4_2294 at 2612.106 kb on - strand, within Pf1N1B4_2294 at 2612.207 kb on + strand, within Pf1N1B4_2294 at 2612.208 kb on - strand, within Pf1N1B4_2294 at 2612.253 kb on - strand, within Pf1N1B4_2294 at 2612.294 kb on + strand, within Pf1N1B4_2294 at 2612.295 kb on - strand, within Pf1N1B4_2294 at 2612.396 kb on + strand, within Pf1N1B4_2294 at 2612.396 kb on + strand, within Pf1N1B4_2294 at 2612.451 kb on - strand, within Pf1N1B4_2294 at 2612.498 kb on + strand, within Pf1N1B4_2294 at 2612.498 kb on + strand, within Pf1N1B4_2294 at 2612.813 kb on + strand at 2612.813 kb on + strand at 2612.813 kb on + strand at 2612.813 kb on + strand at 2612.813 kb on + strand at 2612.846 kb on + strand, within Pf1N1B4_2295 at 2612.846 kb on + strand, within Pf1N1B4_2295 at 2612.847 kb on - strand, within Pf1N1B4_2295 at 2612.847 kb on - strand, within Pf1N1B4_2295 at 2613.055 kb on + strand, within Pf1N1B4_2295 at 2613.056 kb on - strand, within Pf1N1B4_2295 at 2613.056 kb on - strand, within Pf1N1B4_2295 at 2613.194 kb on + strand, within Pf1N1B4_2295 at 2613.194 kb on + strand, within Pf1N1B4_2295 at 2613.195 kb on - strand, within Pf1N1B4_2295 at 2613.296 kb on + strand, within Pf1N1B4_2295 at 2613.296 kb on + strand, within Pf1N1B4_2295 at 2613.296 kb on + strand, within Pf1N1B4_2295 at 2613.297 kb on - strand, within Pf1N1B4_2295 at 2613.297 kb on - strand, within Pf1N1B4_2295 at 2613.297 kb on - strand, within Pf1N1B4_2295 at 2613.297 kb on - strand, within Pf1N1B4_2295 at 2613.329 kb on + strand, within Pf1N1B4_2295 at 2613.330 kb on - strand, within Pf1N1B4_2295 at 2613.330 kb on - strand, within Pf1N1B4_2295 at 2613.330 kb on - strand, within Pf1N1B4_2295 at 2613.569 kb on + strand, within Pf1N1B4_2295 at 2613.569 kb on + strand, within Pf1N1B4_2295 at 2613.569 kb on + strand, within Pf1N1B4_2295 at 2613.570 kb on - strand, within Pf1N1B4_2295 at 2613.570 kb on - strand, within Pf1N1B4_2295 at 2613.571 kb on + strand, within Pf1N1B4_2295 at 2613.572 kb on - strand, within Pf1N1B4_2295 at 2613.639 kb on - strand, within Pf1N1B4_2295 at 2613.639 kb on - strand, within Pf1N1B4_2295 at 2613.720 kb on - strand at 2613.752 kb on + strand at 2613.752 kb on + strand at 2613.752 kb on + strand at 2613.752 kb on + strand at 2613.752 kb on + strand at 2613.752 kb on + strand at 2613.752 kb on + strand at 2613.752 kb on + strand at 2613.753 kb on - strand at 2613.753 kb on - strand at 2613.753 kb on - strand at 2613.753 kb on - strand at 2613.753 kb on - strand at 2613.907 kb on + strand at 2613.939 kb on + strand at 2613.939 kb on + strand at 2613.939 kb on + strand at 2613.939 kb on + strand at 2614.140 kb on + strand, within Pf1N1B4_2296 at 2614.140 kb on + strand, within Pf1N1B4_2296 at 2614.255 kb on + strand, within Pf1N1B4_2296 at 2614.256 kb on - strand, within Pf1N1B4_2296 at 2614.258 kb on - strand, within Pf1N1B4_2296 at 2614.317 kb on + strand, within Pf1N1B4_2296 at 2614.317 kb on + strand, within Pf1N1B4_2296 at 2614.318 kb on - strand, within Pf1N1B4_2296 at 2614.350 kb on + strand, within Pf1N1B4_2296 at 2614.722 kb on + strand, within Pf1N1B4_2296 at 2614.723 kb on - strand, within Pf1N1B4_2296
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 2,611,883 + Pf1N1B4_2294 0.53 -1.2 2,611,884 - Pf1N1B4_2294 0.53 +0.4 2,611,884 - Pf1N1B4_2294 0.53 +1.0 2,611,970 + Pf1N1B4_2294 0.58 -0.9 2,612,036 + +1.4 2,612,065 + Pf1N1B4_2294 0.64 -2.9 2,612,065 + Pf1N1B4_2294 0.64 -0.6 2,612,066 - Pf1N1B4_2294 0.64 -0.1 2,612,066 - Pf1N1B4_2294 0.64 -0.6 2,612,105 + Pf1N1B4_2294 0.66 -1.8 2,612,105 + Pf1N1B4_2294 0.66 +1.0 2,612,106 - Pf1N1B4_2294 0.66 +1.4 2,612,106 - Pf1N1B4_2294 0.66 -0.6 2,612,106 - Pf1N1B4_2294 0.66 -0.0 2,612,207 + Pf1N1B4_2294 0.72 -0.6 2,612,208 - Pf1N1B4_2294 0.72 +1.6 2,612,253 - Pf1N1B4_2294 0.75 -1.6 2,612,294 + Pf1N1B4_2294 0.77 -0.9 2,612,295 - Pf1N1B4_2294 0.77 +2.4 2,612,396 + Pf1N1B4_2294 0.83 +2.4 2,612,396 + Pf1N1B4_2294 0.83 -0.6 2,612,451 - Pf1N1B4_2294 0.86 +0.4 2,612,498 + Pf1N1B4_2294 0.89 -1.9 2,612,498 + Pf1N1B4_2294 0.89 +2.0 2,612,813 + +0.4 2,612,813 + +0.4 2,612,813 + -0.3 2,612,813 + +0.4 2,612,813 + -0.6 2,612,846 + Pf1N1B4_2295 0.11 -0.2 2,612,846 + Pf1N1B4_2295 0.11 -0.9 2,612,847 - Pf1N1B4_2295 0.12 +0.4 2,612,847 - Pf1N1B4_2295 0.12 -2.4 2,613,055 + Pf1N1B4_2295 0.32 -1.6 2,613,056 - Pf1N1B4_2295 0.32 -1.2 2,613,056 - Pf1N1B4_2295 0.32 +0.6 2,613,194 + Pf1N1B4_2295 0.45 -0.6 2,613,194 + Pf1N1B4_2295 0.45 -2.2 2,613,195 - Pf1N1B4_2295 0.45 +1.0 2,613,296 + Pf1N1B4_2295 0.55 -2.1 2,613,296 + Pf1N1B4_2295 0.55 -1.2 2,613,296 + Pf1N1B4_2295 0.55 +0.1 2,613,297 - Pf1N1B4_2295 0.55 -1.2 2,613,297 - Pf1N1B4_2295 0.55 -0.6 2,613,297 - Pf1N1B4_2295 0.55 -0.6 2,613,297 - Pf1N1B4_2295 0.55 +0.4 2,613,329 + Pf1N1B4_2295 0.58 +1.4 2,613,330 - Pf1N1B4_2295 0.59 -1.2 2,613,330 - Pf1N1B4_2295 0.59 -0.6 2,613,330 - Pf1N1B4_2295 0.59 -1.6 2,613,569 + Pf1N1B4_2295 0.82 -0.6 2,613,569 + Pf1N1B4_2295 0.82 -0.9 2,613,569 + Pf1N1B4_2295 0.82 +1.8 2,613,570 - Pf1N1B4_2295 0.82 +1.2 2,613,570 - Pf1N1B4_2295 0.82 +0.4 2,613,571 + Pf1N1B4_2295 0.82 -0.3 2,613,572 - Pf1N1B4_2295 0.82 +0.4 2,613,639 - Pf1N1B4_2295 0.89 -0.6 2,613,639 - Pf1N1B4_2295 0.89 +0.4 2,613,720 - -0.4 2,613,752 + +0.4 2,613,752 + +0.4 2,613,752 + +0.4 2,613,752 + -0.0 2,613,752 + -0.0 2,613,752 + -2.4 2,613,752 + +0.4 2,613,752 + +0.4 2,613,753 - -1.6 2,613,753 - +0.4 2,613,753 - +0.4 2,613,753 - -1.2 2,613,753 - -0.1 2,613,907 + -0.3 2,613,939 + +0.4 2,613,939 + +1.2 2,613,939 + -0.9 2,613,939 + -1.9 2,614,140 + Pf1N1B4_2296 0.23 +0.1 2,614,140 + Pf1N1B4_2296 0.23 -0.2 2,614,255 + Pf1N1B4_2296 0.33 -0.8 2,614,256 - Pf1N1B4_2296 0.33 +0.4 2,614,258 - Pf1N1B4_2296 0.33 -1.6 2,614,317 + Pf1N1B4_2296 0.39 -0.6 2,614,317 + Pf1N1B4_2296 0.39 -0.6 2,614,318 - Pf1N1B4_2296 0.39 -0.9 2,614,350 + Pf1N1B4_2296 0.42 -0.5 2,614,722 + Pf1N1B4_2296 0.74 -1.6 2,614,723 - Pf1N1B4_2296 0.74 +1.4
Or see this region's nucleotide sequence