Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2272 and Pf1N1B4_2273 are separated by 27 nucleotides Pf1N1B4_2273 and Pf1N1B4_2274 are separated by 63 nucleotides
Pf1N1B4_2272 - D-3-phosphoglycerate dehydrogenase (EC 1.1.1.95), at 2,588,243 to 2,589,472
_2272
Pf1N1B4_2273 - hypothetical protein, at 2,589,500 to 2,589,613
_2273
Pf1N1B4_2274 - D-2-hydroxyglutarate dehydrogenase, at 2,589,677 to 2,591,071
_2274
Position (kb)
2589
2590 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2588.515 kb on + strand, within Pf1N1B4_2272 at 2588.530 kb on - strand, within Pf1N1B4_2272 at 2588.678 kb on + strand, within Pf1N1B4_2272 at 2588.678 kb on + strand, within Pf1N1B4_2272 at 2588.679 kb on - strand, within Pf1N1B4_2272 at 2588.679 kb on - strand, within Pf1N1B4_2272 at 2588.934 kb on + strand, within Pf1N1B4_2272 at 2588.935 kb on - strand, within Pf1N1B4_2272 at 2588.935 kb on - strand, within Pf1N1B4_2272 at 2588.935 kb on - strand, within Pf1N1B4_2272 at 2588.935 kb on - strand at 2588.940 kb on + strand, within Pf1N1B4_2272 at 2588.940 kb on + strand, within Pf1N1B4_2272 at 2588.941 kb on - strand, within Pf1N1B4_2272 at 2588.941 kb on - strand, within Pf1N1B4_2272 at 2588.941 kb on - strand, within Pf1N1B4_2272 at 2588.941 kb on - strand, within Pf1N1B4_2272 at 2588.941 kb on - strand, within Pf1N1B4_2272 at 2588.941 kb on - strand, within Pf1N1B4_2272 at 2589.363 kb on + strand at 2589.363 kb on + strand at 2589.363 kb on + strand at 2589.451 kb on + strand at 2589.479 kb on - strand at 2589.573 kb on + strand, within Pf1N1B4_2273 at 2589.574 kb on - strand, within Pf1N1B4_2273 at 2589.594 kb on + strand, within Pf1N1B4_2273 at 2589.599 kb on + strand, within Pf1N1B4_2273 at 2589.599 kb on + strand, within Pf1N1B4_2273 at 2589.599 kb on + strand, within Pf1N1B4_2273 at 2589.599 kb on + strand, within Pf1N1B4_2273 at 2589.599 kb on + strand, within Pf1N1B4_2273 at 2589.599 kb on + strand, within Pf1N1B4_2273 at 2589.600 kb on - strand, within Pf1N1B4_2273 at 2589.600 kb on - strand, within Pf1N1B4_2273 at 2589.600 kb on - strand, within Pf1N1B4_2273 at 2589.600 kb on - strand, within Pf1N1B4_2273 at 2589.600 kb on - strand, within Pf1N1B4_2273 at 2589.600 kb on - strand at 2589.600 kb on - strand, within Pf1N1B4_2273 at 2589.600 kb on - strand, within Pf1N1B4_2273 at 2589.600 kb on - strand, within Pf1N1B4_2273 at 2589.600 kb on - strand, within Pf1N1B4_2273 at 2589.600 kb on - strand, within Pf1N1B4_2273 at 2589.761 kb on + strand at 2589.762 kb on - strand at 2589.766 kb on + strand at 2589.953 kb on + strand, within Pf1N1B4_2274 at 2589.953 kb on + strand, within Pf1N1B4_2274 at 2589.953 kb on + strand, within Pf1N1B4_2274 at 2589.954 kb on - strand, within Pf1N1B4_2274 at 2589.954 kb on - strand, within Pf1N1B4_2274 at 2589.954 kb on - strand, within Pf1N1B4_2274 at 2589.954 kb on - strand, within Pf1N1B4_2274 at 2589.954 kb on - strand, within Pf1N1B4_2274 at 2590.059 kb on - strand, within Pf1N1B4_2274 at 2590.059 kb on - strand, within Pf1N1B4_2274 at 2590.216 kb on + strand, within Pf1N1B4_2274 at 2590.473 kb on + strand, within Pf1N1B4_2274 at 2590.473 kb on + strand, within Pf1N1B4_2274 at 2590.505 kb on + strand, within Pf1N1B4_2274
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 2,588,515 + Pf1N1B4_2272 0.22 -2.4 2,588,530 - Pf1N1B4_2272 0.23 -1.9 2,588,678 + Pf1N1B4_2272 0.35 -0.6 2,588,678 + Pf1N1B4_2272 0.35 -1.9 2,588,679 - Pf1N1B4_2272 0.35 -2.4 2,588,679 - Pf1N1B4_2272 0.35 -1.2 2,588,934 + Pf1N1B4_2272 0.56 -0.6 2,588,935 - Pf1N1B4_2272 0.56 -1.9 2,588,935 - Pf1N1B4_2272 0.56 -1.2 2,588,935 - Pf1N1B4_2272 0.56 -1.9 2,588,935 - +0.4 2,588,940 + Pf1N1B4_2272 0.57 -1.2 2,588,940 + Pf1N1B4_2272 0.57 -2.9 2,588,941 - Pf1N1B4_2272 0.57 -0.6 2,588,941 - Pf1N1B4_2272 0.57 -1.9 2,588,941 - Pf1N1B4_2272 0.57 -2.2 2,588,941 - Pf1N1B4_2272 0.57 +0.4 2,588,941 - Pf1N1B4_2272 0.57 +0.4 2,588,941 - Pf1N1B4_2272 0.57 -1.6 2,589,363 + -1.2 2,589,363 + +0.4 2,589,363 + -1.2 2,589,451 + -1.2 2,589,479 - -2.4 2,589,573 + Pf1N1B4_2273 0.64 -1.6 2,589,574 - Pf1N1B4_2273 0.65 -1.2 2,589,594 + Pf1N1B4_2273 0.82 -1.6 2,589,599 + Pf1N1B4_2273 0.87 -0.6 2,589,599 + Pf1N1B4_2273 0.87 -1.7 2,589,599 + Pf1N1B4_2273 0.87 -1.2 2,589,599 + Pf1N1B4_2273 0.87 -1.6 2,589,599 + Pf1N1B4_2273 0.87 -2.2 2,589,599 + Pf1N1B4_2273 0.87 -3.3 2,589,600 - Pf1N1B4_2273 0.88 -2.4 2,589,600 - Pf1N1B4_2273 0.88 +0.0 2,589,600 - Pf1N1B4_2273 0.88 +0.0 2,589,600 - Pf1N1B4_2273 0.88 +0.4 2,589,600 - Pf1N1B4_2273 0.88 +1.7 2,589,600 - +0.4 2,589,600 - Pf1N1B4_2273 0.88 +0.4 2,589,600 - Pf1N1B4_2273 0.88 -1.2 2,589,600 - Pf1N1B4_2273 0.88 -0.2 2,589,600 - Pf1N1B4_2273 0.88 -0.3 2,589,600 - Pf1N1B4_2273 0.88 +0.1 2,589,761 + +2.0 2,589,762 - -0.7 2,589,766 + -0.9 2,589,953 + Pf1N1B4_2274 0.20 +0.2 2,589,953 + Pf1N1B4_2274 0.20 -0.4 2,589,953 + Pf1N1B4_2274 0.20 -0.4 2,589,954 - Pf1N1B4_2274 0.20 -0.6 2,589,954 - Pf1N1B4_2274 0.20 -0.3 2,589,954 - Pf1N1B4_2274 0.20 -0.2 2,589,954 - Pf1N1B4_2274 0.20 -0.6 2,589,954 - Pf1N1B4_2274 0.20 +1.4 2,590,059 - Pf1N1B4_2274 0.27 -1.4 2,590,059 - Pf1N1B4_2274 0.27 -0.5 2,590,216 + Pf1N1B4_2274 0.39 +0.2 2,590,473 + Pf1N1B4_2274 0.57 -0.3 2,590,473 + Pf1N1B4_2274 0.57 +0.4 2,590,505 + Pf1N1B4_2274 0.59 -0.4
Or see this region's nucleotide sequence