Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2250 and Pf1N1B4_2251 overlap by 4 nucleotides Pf1N1B4_2251 and Pf1N1B4_2252 are separated by 34 nucleotides Pf1N1B4_2252 and Pf1N1B4_2253 are separated by 152 nucleotides
Pf1N1B4_2250 - Alkanesulfonates transport system permease protein, at 2,566,731 to 2,567,513
_2250
Pf1N1B4_2251 - Alkanesulfonates ABC transporter ATP-binding protein / Sulfonate ABC transporter, ATP-binding subunit SsuB, at 2,567,510 to 2,568,316
_2251
Pf1N1B4_2252 - Organosulfonate utilization protein SsuF, at 2,568,351 to 2,568,566
_2252
Pf1N1B4_2253 - Transcriptional regulator, TetR family, at 2,568,719 to 2,569,351
_2253
Position (kb)
2568
2569 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2567.409 kb on + strand, within Pf1N1B4_2250 at 2567.409 kb on + strand, within Pf1N1B4_2250 at 2567.409 kb on + strand, within Pf1N1B4_2250 at 2567.671 kb on + strand, within Pf1N1B4_2251 at 2567.671 kb on + strand, within Pf1N1B4_2251 at 2567.672 kb on - strand, within Pf1N1B4_2251 at 2568.293 kb on - strand at 2568.314 kb on + strand at 2568.315 kb on - strand at 2568.315 kb on - strand at 2568.315 kb on - strand at 2568.319 kb on + strand at 2568.319 kb on + strand at 2568.319 kb on + strand at 2568.320 kb on - strand at 2568.320 kb on - strand at 2568.320 kb on - strand at 2568.320 kb on - strand at 2568.320 kb on - strand at 2568.356 kb on + strand at 2568.357 kb on - strand at 2568.357 kb on - strand at 2568.378 kb on - strand, within Pf1N1B4_2252 at 2568.474 kb on - strand, within Pf1N1B4_2252 at 2568.520 kb on + strand, within Pf1N1B4_2252 at 2568.520 kb on + strand, within Pf1N1B4_2252 at 2568.520 kb on + strand, within Pf1N1B4_2252 at 2568.521 kb on - strand, within Pf1N1B4_2252 at 2568.564 kb on + strand at 2568.564 kb on + strand at 2568.564 kb on + strand at 2568.564 kb on + strand at 2568.564 kb on + strand at 2568.565 kb on - strand at 2568.576 kb on + strand at 2568.741 kb on + strand at 2568.742 kb on - strand at 2568.742 kb on - strand at 2568.742 kb on - strand at 2568.754 kb on - strand at 2568.754 kb on - strand at 2568.754 kb on - strand at 2568.765 kb on + strand at 2568.765 kb on + strand at 2568.766 kb on - strand at 2568.766 kb on - strand at 2568.766 kb on - strand at 2568.840 kb on + strand, within Pf1N1B4_2253 at 2568.840 kb on + strand, within Pf1N1B4_2253 at 2568.840 kb on + strand, within Pf1N1B4_2253 at 2568.840 kb on + strand, within Pf1N1B4_2253 at 2568.840 kb on + strand, within Pf1N1B4_2253 at 2568.840 kb on + strand, within Pf1N1B4_2253 at 2568.840 kb on + strand at 2568.840 kb on + strand, within Pf1N1B4_2253 at 2568.840 kb on + strand, within Pf1N1B4_2253 at 2568.840 kb on + strand, within Pf1N1B4_2253 at 2568.840 kb on + strand, within Pf1N1B4_2253 at 2568.840 kb on + strand, within Pf1N1B4_2253 at 2568.840 kb on + strand, within Pf1N1B4_2253 at 2568.840 kb on + strand, within Pf1N1B4_2253 at 2568.841 kb on - strand, within Pf1N1B4_2253 at 2568.841 kb on - strand, within Pf1N1B4_2253 at 2568.841 kb on - strand, within Pf1N1B4_2253 at 2568.841 kb on - strand, within Pf1N1B4_2253 at 2568.841 kb on - strand, within Pf1N1B4_2253 at 2568.841 kb on - strand at 2568.841 kb on - strand, within Pf1N1B4_2253 at 2568.841 kb on - strand, within Pf1N1B4_2253 at 2568.841 kb on - strand, within Pf1N1B4_2253 at 2569.035 kb on + strand, within Pf1N1B4_2253 at 2569.035 kb on + strand, within Pf1N1B4_2253 at 2569.035 kb on + strand, within Pf1N1B4_2253 at 2569.036 kb on - strand, within Pf1N1B4_2253 at 2569.233 kb on + strand, within Pf1N1B4_2253 at 2569.381 kb on + strand at 2569.381 kb on + strand at 2569.381 kb on + strand at 2569.382 kb on - strand at 2569.382 kb on - strand at 2569.382 kb on - strand at 2569.429 kb on + strand at 2569.464 kb on + strand at 2569.464 kb on + strand at 2569.464 kb on + strand at 2569.464 kb on + strand at 2569.464 kb on + strand at 2569.464 kb on + strand at 2569.464 kb on + strand at 2569.464 kb on + strand at 2569.464 kb on + strand at 2569.464 kb on + strand at 2569.464 kb on + strand at 2569.464 kb on + strand at 2569.464 kb on + strand at 2569.465 kb on - strand at 2569.465 kb on - strand at 2569.465 kb on - strand at 2569.465 kb on - strand at 2569.465 kb on - strand at 2569.465 kb on - strand at 2569.465 kb on - strand at 2569.465 kb on - strand at 2569.465 kb on - strand at 2569.465 kb on - strand at 2569.465 kb on - strand at 2569.465 kb on - strand at 2569.465 kb on - strand at 2569.465 kb on - strand at 2569.465 kb on - strand at 2569.494 kb on - strand at 2569.524 kb on + strand at 2569.524 kb on + strand at 2569.545 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 2,567,409 + Pf1N1B4_2250 0.87 -1.7 2,567,409 + Pf1N1B4_2250 0.87 +1.0 2,567,409 + Pf1N1B4_2250 0.87 +0.4 2,567,671 + Pf1N1B4_2251 0.20 -1.1 2,567,671 + Pf1N1B4_2251 0.20 -0.3 2,567,672 - Pf1N1B4_2251 0.20 -0.7 2,568,293 - -0.4 2,568,314 + -0.3 2,568,315 - +1.2 2,568,315 - +0.0 2,568,315 - -1.9 2,568,319 + +0.4 2,568,319 + -0.9 2,568,319 + -0.0 2,568,320 - -1.1 2,568,320 - -1.1 2,568,320 - +0.2 2,568,320 - +0.8 2,568,320 - +0.5 2,568,356 + -0.6 2,568,357 - +2.0 2,568,357 - +0.4 2,568,378 - Pf1N1B4_2252 0.12 -1.0 2,568,474 - Pf1N1B4_2252 0.57 -1.4 2,568,520 + Pf1N1B4_2252 0.78 -0.8 2,568,520 + Pf1N1B4_2252 0.78 -1.6 2,568,520 + Pf1N1B4_2252 0.78 +0.4 2,568,521 - Pf1N1B4_2252 0.79 -0.6 2,568,564 + -0.1 2,568,564 + -0.3 2,568,564 + +1.0 2,568,564 + -0.1 2,568,564 + +0.4 2,568,565 - -0.6 2,568,576 + -1.1 2,568,741 + -1.1 2,568,742 - +2.0 2,568,742 - -0.6 2,568,742 - +1.0 2,568,754 - -1.1 2,568,754 - -0.4 2,568,754 - -0.9 2,568,765 + -1.0 2,568,765 + -1.1 2,568,766 - -0.0 2,568,766 - +0.4 2,568,766 - +0.4 2,568,840 + Pf1N1B4_2253 0.19 +0.4 2,568,840 + Pf1N1B4_2253 0.19 +0.1 2,568,840 + Pf1N1B4_2253 0.19 +2.0 2,568,840 + Pf1N1B4_2253 0.19 +1.0 2,568,840 + Pf1N1B4_2253 0.19 -0.7 2,568,840 + Pf1N1B4_2253 0.19 +2.4 2,568,840 + +1.4 2,568,840 + Pf1N1B4_2253 0.19 +1.8 2,568,840 + Pf1N1B4_2253 0.19 +0.4 2,568,840 + Pf1N1B4_2253 0.19 +0.4 2,568,840 + Pf1N1B4_2253 0.19 +1.4 2,568,840 + Pf1N1B4_2253 0.19 -0.1 2,568,840 + Pf1N1B4_2253 0.19 -1.1 2,568,840 + Pf1N1B4_2253 0.19 -1.1 2,568,841 - Pf1N1B4_2253 0.19 -0.6 2,568,841 - Pf1N1B4_2253 0.19 -1.6 2,568,841 - Pf1N1B4_2253 0.19 +0.4 2,568,841 - Pf1N1B4_2253 0.19 +0.4 2,568,841 - Pf1N1B4_2253 0.19 -0.1 2,568,841 - +0.4 2,568,841 - Pf1N1B4_2253 0.19 -2.4 2,568,841 - Pf1N1B4_2253 0.19 -0.6 2,568,841 - Pf1N1B4_2253 0.19 +0.4 2,569,035 + Pf1N1B4_2253 0.50 -1.1 2,569,035 + Pf1N1B4_2253 0.50 -0.3 2,569,035 + Pf1N1B4_2253 0.50 -1.9 2,569,036 - Pf1N1B4_2253 0.50 -0.8 2,569,233 + Pf1N1B4_2253 0.81 +1.4 2,569,381 + -0.4 2,569,381 + +0.8 2,569,381 + -0.7 2,569,382 - +0.1 2,569,382 - -1.0 2,569,382 - +2.3 2,569,429 + -1.1 2,569,464 + -1.1 2,569,464 + -0.6 2,569,464 + -1.6 2,569,464 + +0.4 2,569,464 + -0.9 2,569,464 + +0.4 2,569,464 + -0.8 2,569,464 + -2.1 2,569,464 + +0.4 2,569,464 + +0.2 2,569,464 + +1.4 2,569,464 + -0.2 2,569,464 + -1.1 2,569,465 - -0.1 2,569,465 - +0.0 2,569,465 - -0.4 2,569,465 - +0.0 2,569,465 - +0.1 2,569,465 - +0.4 2,569,465 - -1.1 2,569,465 - -0.6 2,569,465 - -0.9 2,569,465 - +1.4 2,569,465 - +0.4 2,569,465 - -0.1 2,569,465 - +0.1 2,569,465 - +0.1 2,569,465 - +1.0 2,569,494 - +1.3 2,569,524 + -1.1 2,569,524 + -1.6 2,569,545 + +1.0
Or see this region's nucleotide sequence