Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2222 and Pf1N1B4_2223 are separated by 139 nucleotides Pf1N1B4_2223 and Pf1N1B4_2224 are separated by 200 nucleotides Pf1N1B4_2224 and Pf1N1B4_2225 are separated by 34 nucleotides Pf1N1B4_2225 and Pf1N1B4_2226 are separated by 294 nucleotides
Pf1N1B4_2222 - Flagellar biosynthesis protein FliL, at 2,536,472 to 2,536,879
_2222
Pf1N1B4_2223 - FIG006231: RNA-binding protein, at 2,537,019 to 2,537,471
_2223
Pf1N1B4_2224 - FIG00953390: hypothetical protein, at 2,537,672 to 2,537,821
_2224
Pf1N1B4_2225 - 5-formyltetrahydrofolate cyclo-ligase (EC 6.3.3.2), at 2,537,856 to 2,538,461
_2225
Pf1N1B4_2226 - Z-ring-associated protein ZapA, at 2,538,756 to 2,539,073
_2226
Position (kb)
2537
2538 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2536.957 kb on - strand at 2537.020 kb on + strand at 2537.134 kb on + strand, within Pf1N1B4_2223 at 2537.135 kb on - strand, within Pf1N1B4_2223 at 2537.227 kb on + strand, within Pf1N1B4_2223 at 2537.227 kb on + strand, within Pf1N1B4_2223 at 2537.227 kb on + strand, within Pf1N1B4_2223 at 2537.228 kb on - strand, within Pf1N1B4_2223 at 2537.228 kb on - strand, within Pf1N1B4_2223 at 2537.324 kb on - strand, within Pf1N1B4_2223 at 2537.324 kb on - strand, within Pf1N1B4_2223 at 2537.324 kb on - strand, within Pf1N1B4_2223 at 2537.377 kb on + strand, within Pf1N1B4_2223 at 2537.465 kb on - strand at 2537.517 kb on + strand at 2537.517 kb on + strand at 2537.517 kb on + strand at 2537.518 kb on - strand at 2537.589 kb on + strand at 2537.589 kb on + strand at 2537.590 kb on - strand at 2537.613 kb on + strand at 2537.619 kb on + strand at 2537.619 kb on + strand at 2537.620 kb on - strand at 2537.691 kb on + strand, within Pf1N1B4_2224 at 2537.691 kb on + strand, within Pf1N1B4_2224 at 2537.691 kb on + strand, within Pf1N1B4_2224 at 2537.691 kb on + strand, within Pf1N1B4_2224 at 2537.692 kb on - strand, within Pf1N1B4_2224 at 2537.692 kb on - strand, within Pf1N1B4_2224 at 2537.708 kb on - strand, within Pf1N1B4_2224 at 2537.858 kb on - strand at 2537.869 kb on + strand at 2537.869 kb on + strand at 2537.869 kb on + strand at 2537.869 kb on + strand at 2537.869 kb on + strand at 2537.869 kb on + strand at 2537.870 kb on - strand at 2537.870 kb on - strand at 2537.870 kb on - strand at 2537.870 kb on - strand at 2537.870 kb on - strand at 2537.904 kb on + strand at 2537.904 kb on + strand at 2537.904 kb on + strand at 2537.904 kb on + strand at 2537.905 kb on - strand at 2537.905 kb on - strand at 2538.001 kb on + strand at 2538.002 kb on - strand, within Pf1N1B4_2225 at 2538.002 kb on - strand, within Pf1N1B4_2225 at 2538.019 kb on + strand, within Pf1N1B4_2225 at 2538.019 kb on + strand, within Pf1N1B4_2225 at 2538.019 kb on + strand, within Pf1N1B4_2225 at 2538.020 kb on - strand, within Pf1N1B4_2225 at 2538.020 kb on - strand, within Pf1N1B4_2225 at 2538.020 kb on - strand, within Pf1N1B4_2225 at 2538.020 kb on - strand, within Pf1N1B4_2225 at 2538.229 kb on + strand, within Pf1N1B4_2225 at 2538.229 kb on + strand, within Pf1N1B4_2225 at 2538.229 kb on + strand, within Pf1N1B4_2225 at 2538.229 kb on + strand, within Pf1N1B4_2225 at 2538.229 kb on + strand, within Pf1N1B4_2225 at 2538.229 kb on + strand, within Pf1N1B4_2225 at 2538.229 kb on + strand, within Pf1N1B4_2225 at 2538.229 kb on + strand, within Pf1N1B4_2225 at 2538.229 kb on + strand, within Pf1N1B4_2225 at 2538.229 kb on + strand, within Pf1N1B4_2225 at 2538.229 kb on + strand, within Pf1N1B4_2225 at 2538.229 kb on + strand, within Pf1N1B4_2225 at 2538.229 kb on + strand, within Pf1N1B4_2225 at 2538.229 kb on + strand, within Pf1N1B4_2225 at 2538.229 kb on + strand, within Pf1N1B4_2225 at 2538.229 kb on + strand, within Pf1N1B4_2225 at 2538.230 kb on - strand, within Pf1N1B4_2225 at 2538.230 kb on - strand, within Pf1N1B4_2225 at 2538.230 kb on - strand, within Pf1N1B4_2225 at 2538.230 kb on - strand at 2538.230 kb on - strand, within Pf1N1B4_2225 at 2538.230 kb on - strand, within Pf1N1B4_2225 at 2538.230 kb on - strand, within Pf1N1B4_2225 at 2538.230 kb on - strand, within Pf1N1B4_2225 at 2538.230 kb on - strand, within Pf1N1B4_2225 at 2538.230 kb on - strand, within Pf1N1B4_2225 at 2538.230 kb on - strand, within Pf1N1B4_2225 at 2538.230 kb on - strand, within Pf1N1B4_2225 at 2538.230 kb on - strand, within Pf1N1B4_2225 at 2538.230 kb on - strand, within Pf1N1B4_2225 at 2538.230 kb on - strand, within Pf1N1B4_2225 at 2538.230 kb on - strand, within Pf1N1B4_2225 at 2538.230 kb on - strand, within Pf1N1B4_2225 at 2538.230 kb on - strand, within Pf1N1B4_2225 at 2538.230 kb on - strand, within Pf1N1B4_2225 at 2538.230 kb on - strand, within Pf1N1B4_2225 at 2538.230 kb on - strand, within Pf1N1B4_2225 at 2538.230 kb on - strand, within Pf1N1B4_2225 at 2538.230 kb on - strand, within Pf1N1B4_2225 at 2538.230 kb on - strand at 2538.230 kb on - strand, within Pf1N1B4_2225 at 2538.230 kb on - strand, within Pf1N1B4_2225 at 2538.233 kb on + strand, within Pf1N1B4_2225 at 2538.233 kb on + strand, within Pf1N1B4_2225 at 2538.233 kb on + strand, within Pf1N1B4_2225 at 2538.233 kb on + strand, within Pf1N1B4_2225 at 2538.233 kb on + strand, within Pf1N1B4_2225 at 2538.233 kb on + strand, within Pf1N1B4_2225 at 2538.233 kb on + strand, within Pf1N1B4_2225 at 2538.233 kb on + strand, within Pf1N1B4_2225 at 2538.234 kb on - strand, within Pf1N1B4_2225 at 2538.234 kb on - strand, within Pf1N1B4_2225 at 2538.234 kb on - strand, within Pf1N1B4_2225 at 2538.234 kb on - strand, within Pf1N1B4_2225 at 2538.234 kb on - strand, within Pf1N1B4_2225 at 2538.234 kb on - strand, within Pf1N1B4_2225 at 2538.234 kb on - strand, within Pf1N1B4_2225 at 2538.301 kb on + strand, within Pf1N1B4_2225 at 2538.301 kb on + strand, within Pf1N1B4_2225 at 2538.301 kb on + strand, within Pf1N1B4_2225 at 2538.302 kb on - strand, within Pf1N1B4_2225 at 2538.302 kb on - strand, within Pf1N1B4_2225 at 2538.302 kb on - strand, within Pf1N1B4_2225 at 2538.302 kb on - strand, within Pf1N1B4_2225 at 2538.353 kb on + strand, within Pf1N1B4_2225 at 2538.353 kb on + strand, within Pf1N1B4_2225 at 2538.353 kb on + strand, within Pf1N1B4_2225 at 2538.353 kb on + strand, within Pf1N1B4_2225 at 2538.354 kb on - strand, within Pf1N1B4_2225 at 2538.354 kb on - strand, within Pf1N1B4_2225 at 2538.354 kb on - strand, within Pf1N1B4_2225 at 2538.354 kb on - strand, within Pf1N1B4_2225 at 2538.354 kb on - strand, within Pf1N1B4_2225 at 2538.355 kb on + strand, within Pf1N1B4_2225 at 2538.355 kb on + strand, within Pf1N1B4_2225 at 2538.355 kb on + strand, within Pf1N1B4_2225 at 2538.355 kb on + strand, within Pf1N1B4_2225 at 2538.355 kb on + strand, within Pf1N1B4_2225 at 2538.355 kb on + strand, within Pf1N1B4_2225 at 2538.355 kb on + strand, within Pf1N1B4_2225 at 2538.355 kb on + strand, within Pf1N1B4_2225 at 2538.355 kb on + strand, within Pf1N1B4_2225 at 2538.355 kb on + strand, within Pf1N1B4_2225 at 2538.356 kb on - strand, within Pf1N1B4_2225 at 2538.356 kb on - strand, within Pf1N1B4_2225 at 2538.356 kb on - strand, within Pf1N1B4_2225 at 2538.356 kb on - strand, within Pf1N1B4_2225 at 2538.356 kb on - strand, within Pf1N1B4_2225 at 2538.356 kb on - strand, within Pf1N1B4_2225 at 2538.356 kb on - strand, within Pf1N1B4_2225 at 2538.487 kb on - strand at 2538.524 kb on + strand at 2538.524 kb on + strand at 2538.524 kb on + strand at 2538.525 kb on - strand at 2538.525 kb on - strand at 2538.545 kb on + strand at 2538.545 kb on + strand at 2538.545 kb on + strand at 2538.545 kb on + strand at 2538.545 kb on + strand at 2538.545 kb on + strand at 2538.546 kb on - strand at 2538.546 kb on - strand at 2538.575 kb on + strand at 2538.576 kb on - strand at 2538.613 kb on + strand at 2538.613 kb on + strand at 2538.614 kb on - strand at 2538.614 kb on - strand at 2538.635 kb on - strand at 2538.733 kb on + strand at 2538.733 kb on + strand at 2538.733 kb on + strand at 2538.734 kb on - strand at 2538.734 kb on - strand at 2538.734 kb on - strand at 2538.734 kb on - strand at 2538.734 kb on - strand at 2538.734 kb on - strand at 2538.734 kb on - strand at 2538.735 kb on + strand at 2538.735 kb on + strand at 2538.736 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 2,536,957 - -0.5 2,537,020 + +0.5 2,537,134 + Pf1N1B4_2223 0.25 -0.6 2,537,135 - Pf1N1B4_2223 0.26 -0.4 2,537,227 + Pf1N1B4_2223 0.46 -0.3 2,537,227 + Pf1N1B4_2223 0.46 -1.0 2,537,227 + Pf1N1B4_2223 0.46 -1.5 2,537,228 - Pf1N1B4_2223 0.46 +0.5 2,537,228 - Pf1N1B4_2223 0.46 -0.7 2,537,324 - Pf1N1B4_2223 0.67 -1.1 2,537,324 - Pf1N1B4_2223 0.67 -0.8 2,537,324 - Pf1N1B4_2223 0.67 -0.5 2,537,377 + Pf1N1B4_2223 0.79 +0.0 2,537,465 - +0.9 2,537,517 + -0.7 2,537,517 + +0.3 2,537,517 + -1.3 2,537,518 - +2.0 2,537,589 + +2.4 2,537,589 + -1.6 2,537,590 - +0.4 2,537,613 + -1.4 2,537,619 + -0.6 2,537,619 + +0.3 2,537,620 - +1.7 2,537,691 + Pf1N1B4_2224 0.13 -0.6 2,537,691 + Pf1N1B4_2224 0.13 -1.1 2,537,691 + Pf1N1B4_2224 0.13 +2.0 2,537,691 + Pf1N1B4_2224 0.13 +0.7 2,537,692 - Pf1N1B4_2224 0.13 +1.4 2,537,692 - Pf1N1B4_2224 0.13 +0.8 2,537,708 - Pf1N1B4_2224 0.24 +0.4 2,537,858 - +0.4 2,537,869 + +0.4 2,537,869 + -1.6 2,537,869 + -1.1 2,537,869 + +0.4 2,537,869 + -1.3 2,537,869 + -0.4 2,537,870 - -0.6 2,537,870 - -0.7 2,537,870 - -1.9 2,537,870 - -0.6 2,537,870 - -0.3 2,537,904 + -1.1 2,537,904 + -1.1 2,537,904 + -1.3 2,537,904 + +0.4 2,537,905 - +0.2 2,537,905 - +0.4 2,538,001 + -0.6 2,538,002 - Pf1N1B4_2225 0.24 -0.6 2,538,002 - Pf1N1B4_2225 0.24 -1.6 2,538,019 + Pf1N1B4_2225 0.27 +1.0 2,538,019 + Pf1N1B4_2225 0.27 -0.6 2,538,019 + Pf1N1B4_2225 0.27 -0.6 2,538,020 - Pf1N1B4_2225 0.27 -1.1 2,538,020 - Pf1N1B4_2225 0.27 -0.8 2,538,020 - Pf1N1B4_2225 0.27 -1.9 2,538,020 - Pf1N1B4_2225 0.27 -2.1 2,538,229 + Pf1N1B4_2225 0.62 -1.9 2,538,229 + Pf1N1B4_2225 0.62 -1.9 2,538,229 + Pf1N1B4_2225 0.62 -0.7 2,538,229 + Pf1N1B4_2225 0.62 -2.7 2,538,229 + Pf1N1B4_2225 0.62 -0.6 2,538,229 + Pf1N1B4_2225 0.62 -0.8 2,538,229 + Pf1N1B4_2225 0.62 -0.1 2,538,229 + Pf1N1B4_2225 0.62 -1.9 2,538,229 + Pf1N1B4_2225 0.62 -0.6 2,538,229 + Pf1N1B4_2225 0.62 +1.0 2,538,229 + Pf1N1B4_2225 0.62 -0.1 2,538,229 + Pf1N1B4_2225 0.62 -1.9 2,538,229 + Pf1N1B4_2225 0.62 -0.9 2,538,229 + Pf1N1B4_2225 0.62 -1.1 2,538,229 + Pf1N1B4_2225 0.62 -1.0 2,538,229 + Pf1N1B4_2225 0.62 -1.4 2,538,230 - Pf1N1B4_2225 0.62 -0.9 2,538,230 - Pf1N1B4_2225 0.62 -0.6 2,538,230 - Pf1N1B4_2225 0.62 +0.4 2,538,230 - -1.1 2,538,230 - Pf1N1B4_2225 0.62 -1.7 2,538,230 - Pf1N1B4_2225 0.62 -0.0 2,538,230 - Pf1N1B4_2225 0.62 -1.6 2,538,230 - Pf1N1B4_2225 0.62 -2.7 2,538,230 - Pf1N1B4_2225 0.62 -1.6 2,538,230 - Pf1N1B4_2225 0.62 -1.0 2,538,230 - Pf1N1B4_2225 0.62 -0.9 2,538,230 - Pf1N1B4_2225 0.62 +0.4 2,538,230 - Pf1N1B4_2225 0.62 -1.6 2,538,230 - Pf1N1B4_2225 0.62 +1.0 2,538,230 - Pf1N1B4_2225 0.62 -0.6 2,538,230 - Pf1N1B4_2225 0.62 -0.7 2,538,230 - Pf1N1B4_2225 0.62 +1.2 2,538,230 - Pf1N1B4_2225 0.62 +0.9 2,538,230 - Pf1N1B4_2225 0.62 -0.6 2,538,230 - Pf1N1B4_2225 0.62 -1.7 2,538,230 - Pf1N1B4_2225 0.62 -0.9 2,538,230 - Pf1N1B4_2225 0.62 -0.8 2,538,230 - Pf1N1B4_2225 0.62 -1.5 2,538,230 - -1.1 2,538,230 - Pf1N1B4_2225 0.62 -0.6 2,538,230 - Pf1N1B4_2225 0.62 -1.2 2,538,233 + Pf1N1B4_2225 0.62 -0.6 2,538,233 + Pf1N1B4_2225 0.62 +0.8 2,538,233 + Pf1N1B4_2225 0.62 -0.6 2,538,233 + Pf1N1B4_2225 0.62 -0.9 2,538,233 + Pf1N1B4_2225 0.62 -1.1 2,538,233 + Pf1N1B4_2225 0.62 -0.1 2,538,233 + Pf1N1B4_2225 0.62 -1.1 2,538,233 + Pf1N1B4_2225 0.62 -1.6 2,538,234 - Pf1N1B4_2225 0.62 -0.1 2,538,234 - Pf1N1B4_2225 0.62 -0.1 2,538,234 - Pf1N1B4_2225 0.62 -0.9 2,538,234 - Pf1N1B4_2225 0.62 -0.1 2,538,234 - Pf1N1B4_2225 0.62 +0.9 2,538,234 - Pf1N1B4_2225 0.62 -2.1 2,538,234 - Pf1N1B4_2225 0.62 +0.4 2,538,301 + Pf1N1B4_2225 0.73 -2.4 2,538,301 + Pf1N1B4_2225 0.73 -2.4 2,538,301 + Pf1N1B4_2225 0.73 +1.4 2,538,302 - Pf1N1B4_2225 0.74 -1.1 2,538,302 - Pf1N1B4_2225 0.74 -0.1 2,538,302 - Pf1N1B4_2225 0.74 -1.1 2,538,302 - Pf1N1B4_2225 0.74 -0.6 2,538,353 + Pf1N1B4_2225 0.82 -1.3 2,538,353 + Pf1N1B4_2225 0.82 -0.6 2,538,353 + Pf1N1B4_2225 0.82 +0.4 2,538,353 + Pf1N1B4_2225 0.82 -0.6 2,538,354 - Pf1N1B4_2225 0.82 +0.4 2,538,354 - Pf1N1B4_2225 0.82 -1.9 2,538,354 - Pf1N1B4_2225 0.82 -1.1 2,538,354 - Pf1N1B4_2225 0.82 -0.6 2,538,354 - Pf1N1B4_2225 0.82 -1.1 2,538,355 + Pf1N1B4_2225 0.82 +0.0 2,538,355 + Pf1N1B4_2225 0.82 -1.6 2,538,355 + Pf1N1B4_2225 0.82 +0.1 2,538,355 + Pf1N1B4_2225 0.82 -0.6 2,538,355 + Pf1N1B4_2225 0.82 -0.6 2,538,355 + Pf1N1B4_2225 0.82 -1.1 2,538,355 + Pf1N1B4_2225 0.82 -2.4 2,538,355 + Pf1N1B4_2225 0.82 +0.4 2,538,355 + Pf1N1B4_2225 0.82 -0.6 2,538,355 + Pf1N1B4_2225 0.82 -1.7 2,538,356 - Pf1N1B4_2225 0.83 +0.4 2,538,356 - Pf1N1B4_2225 0.83 -0.3 2,538,356 - Pf1N1B4_2225 0.83 -1.1 2,538,356 - Pf1N1B4_2225 0.83 -0.1 2,538,356 - Pf1N1B4_2225 0.83 -0.3 2,538,356 - Pf1N1B4_2225 0.83 +1.4 2,538,356 - Pf1N1B4_2225 0.83 -0.1 2,538,487 - -1.1 2,538,524 + -0.6 2,538,524 + +0.4 2,538,524 + +0.9 2,538,525 - -1.6 2,538,525 - -0.6 2,538,545 + -0.6 2,538,545 + +0.4 2,538,545 + +1.4 2,538,545 + -0.7 2,538,545 + +1.0 2,538,545 + +0.7 2,538,546 - +1.0 2,538,546 - -0.1 2,538,575 + -1.3 2,538,576 - +0.4 2,538,613 + -0.6 2,538,613 + -0.5 2,538,614 - +0.4 2,538,614 - -0.6 2,538,635 - -1.9 2,538,733 + -0.9 2,538,733 + -0.9 2,538,733 + +0.4 2,538,734 - +0.9 2,538,734 - -0.7 2,538,734 - -1.0 2,538,734 - +1.0 2,538,734 - +0.4 2,538,734 - -0.6 2,538,734 - -0.6 2,538,735 + -0.3 2,538,735 + +0.0 2,538,736 - -0.6
Or see this region's nucleotide sequence