Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2209 and Pf1N1B4_2210 are separated by 208 nucleotides Pf1N1B4_2210 and Pf1N1B4_2211 overlap by 14 nucleotides Pf1N1B4_2211 and Pf1N1B4_2212 overlap by 4 nucleotides Pf1N1B4_2212 and Pf1N1B4_2213 are separated by 19 nucleotides
Pf1N1B4_2209 - Exopolyphosphatase (EC 3.6.1.11), at 2,522,879 to 2,524,381
_2209
Pf1N1B4_2210 - Glutamate transport ATP-binding protein, at 2,524,590 to 2,525,327
_2210
Pf1N1B4_2211 - Putative amino acid ABC transporter, permease protein, at 2,525,314 to 2,525,964
_2211
Pf1N1B4_2212 - amino acid ABC transporter, permease protein, at 2,525,961 to 2,526,632
_2212
Pf1N1B4_2213 - ABC-type amino acid transport/signal transduction systems, periplasmic component/domain, at 2,526,652 to 2,527,437
_2213
Position (kb)
2525
2526 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2524.512 kb on + strand at 2524.548 kb on + strand at 2524.548 kb on + strand at 2524.549 kb on - strand at 2524.656 kb on + strand at 2524.656 kb on + strand at 2524.656 kb on + strand at 2524.657 kb on - strand at 2524.657 kb on - strand at 2524.657 kb on - strand at 2524.657 kb on - strand at 2525.131 kb on + strand, within Pf1N1B4_2210 at 2525.132 kb on - strand, within Pf1N1B4_2210 at 2525.132 kb on - strand at 2525.293 kb on + strand at 2525.293 kb on + strand at 2525.293 kb on + strand at 2525.293 kb on + strand at 2525.293 kb on + strand at 2525.293 kb on + strand at 2525.294 kb on - strand at 2525.294 kb on - strand at 2525.294 kb on - strand at 2525.294 kb on - strand at 2525.294 kb on - strand at 2525.294 kb on - strand at 2525.294 kb on - strand at 2525.297 kb on + strand at 2525.297 kb on + strand at 2525.297 kb on + strand at 2525.298 kb on - strand at 2525.298 kb on - strand at 2525.298 kb on - strand at 2525.298 kb on - strand at 2525.298 kb on - strand at 2525.298 kb on - strand at 2525.298 kb on - strand at 2525.298 kb on - strand at 2525.298 kb on - strand at 2525.298 kb on - strand at 2525.315 kb on + strand at 2525.315 kb on + strand at 2525.315 kb on + strand at 2525.316 kb on - strand at 2525.316 kb on - strand at 2525.316 kb on - strand at 2525.316 kb on - strand at 2525.369 kb on + strand at 2525.385 kb on - strand, within Pf1N1B4_2211 at 2525.405 kb on + strand, within Pf1N1B4_2211 at 2525.405 kb on + strand, within Pf1N1B4_2211 at 2525.567 kb on + strand, within Pf1N1B4_2211 at 2525.568 kb on - strand, within Pf1N1B4_2211 at 2525.568 kb on - strand, within Pf1N1B4_2211 at 2525.666 kb on + strand, within Pf1N1B4_2211 at 2525.667 kb on - strand, within Pf1N1B4_2211 at 2525.667 kb on - strand, within Pf1N1B4_2211 at 2525.798 kb on + strand, within Pf1N1B4_2211 at 2525.798 kb on + strand, within Pf1N1B4_2211 at 2525.798 kb on + strand, within Pf1N1B4_2211 at 2525.798 kb on + strand, within Pf1N1B4_2211 at 2525.798 kb on + strand, within Pf1N1B4_2211 at 2525.799 kb on - strand, within Pf1N1B4_2211 at 2525.799 kb on - strand, within Pf1N1B4_2211 at 2525.799 kb on - strand, within Pf1N1B4_2211 at 2525.799 kb on - strand, within Pf1N1B4_2211 at 2525.880 kb on + strand, within Pf1N1B4_2211 at 2525.982 kb on + strand at 2525.982 kb on + strand at 2525.982 kb on + strand at 2525.982 kb on + strand at 2525.982 kb on + strand at 2525.982 kb on + strand at 2525.982 kb on + strand at 2525.982 kb on + strand at 2525.982 kb on + strand at 2525.982 kb on + strand at 2525.982 kb on + strand at 2525.982 kb on + strand at 2525.982 kb on + strand at 2525.982 kb on + strand at 2525.982 kb on + strand at 2525.982 kb on + strand at 2525.982 kb on + strand at 2525.982 kb on + strand at 2525.983 kb on - strand at 2525.983 kb on - strand at 2525.983 kb on - strand at 2525.983 kb on - strand at 2525.983 kb on - strand at 2525.983 kb on - strand at 2525.983 kb on - strand at 2525.983 kb on - strand at 2525.983 kb on - strand at 2525.983 kb on - strand at 2525.986 kb on + strand at 2525.987 kb on - strand at 2525.987 kb on - strand at 2525.987 kb on - strand at 2525.987 kb on - strand at 2525.987 kb on - strand at 2525.987 kb on - strand at 2526.040 kb on + strand at 2526.040 kb on + strand, within Pf1N1B4_2212 at 2526.040 kb on + strand, within Pf1N1B4_2212 at 2526.040 kb on + strand, within Pf1N1B4_2212 at 2526.040 kb on + strand, within Pf1N1B4_2212 at 2526.041 kb on - strand, within Pf1N1B4_2212 at 2526.041 kb on - strand, within Pf1N1B4_2212 at 2526.041 kb on - strand, within Pf1N1B4_2212 at 2526.041 kb on - strand, within Pf1N1B4_2212 at 2526.041 kb on - strand, within Pf1N1B4_2212 at 2526.041 kb on - strand, within Pf1N1B4_2212 at 2526.061 kb on + strand, within Pf1N1B4_2212 at 2526.061 kb on + strand, within Pf1N1B4_2212 at 2526.061 kb on + strand, within Pf1N1B4_2212 at 2526.061 kb on + strand, within Pf1N1B4_2212 at 2526.061 kb on + strand, within Pf1N1B4_2212 at 2526.061 kb on + strand, within Pf1N1B4_2212 at 2526.062 kb on - strand, within Pf1N1B4_2212 at 2526.062 kb on - strand, within Pf1N1B4_2212 at 2526.062 kb on - strand, within Pf1N1B4_2212 at 2526.084 kb on - strand, within Pf1N1B4_2212 at 2526.084 kb on - strand, within Pf1N1B4_2212 at 2526.323 kb on - strand, within Pf1N1B4_2212 at 2526.323 kb on - strand, within Pf1N1B4_2212 at 2526.393 kb on + strand, within Pf1N1B4_2212 at 2526.454 kb on + strand, within Pf1N1B4_2212 at 2526.455 kb on - strand, within Pf1N1B4_2212 at 2526.455 kb on - strand, within Pf1N1B4_2212 at 2526.625 kb on + strand at 2526.625 kb on + strand at 2526.626 kb on - strand at 2526.632 kb on + strand at 2526.632 kb on + strand at 2526.632 kb on + strand at 2526.632 kb on + strand at 2526.633 kb on - strand at 2526.633 kb on - strand at 2526.633 kb on - strand at 2526.633 kb on - strand at 2526.633 kb on - strand at 2526.785 kb on + strand, within Pf1N1B4_2213 at 2526.786 kb on - strand, within Pf1N1B4_2213 at 2526.786 kb on - strand, within Pf1N1B4_2213 at 2526.786 kb on - strand, within Pf1N1B4_2213 at 2526.902 kb on + strand, within Pf1N1B4_2213 at 2526.902 kb on + strand, within Pf1N1B4_2213 at 2526.903 kb on - strand, within Pf1N1B4_2213
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 2,524,512 + +0.5 2,524,548 + -1.4 2,524,548 + -0.4 2,524,549 - -1.3 2,524,656 + +0.9 2,524,656 + +1.8 2,524,656 + +0.8 2,524,657 - -1.5 2,524,657 - -1.9 2,524,657 - +0.0 2,524,657 - +0.5 2,525,131 + Pf1N1B4_2210 0.73 +0.2 2,525,132 - Pf1N1B4_2210 0.73 -1.0 2,525,132 - +0.5 2,525,293 + +0.7 2,525,293 + +0.5 2,525,293 + -0.5 2,525,293 + -0.2 2,525,293 + -0.5 2,525,293 + -0.1 2,525,294 - -0.5 2,525,294 - +1.0 2,525,294 - -0.4 2,525,294 - -0.3 2,525,294 - -1.3 2,525,294 - +0.7 2,525,294 - -0.1 2,525,297 + -1.4 2,525,297 + +0.5 2,525,297 + +0.0 2,525,298 - -1.5 2,525,298 - -1.1 2,525,298 - -0.1 2,525,298 - -1.1 2,525,298 - +1.5 2,525,298 - -0.0 2,525,298 - -1.9 2,525,298 - -0.8 2,525,298 - +1.0 2,525,298 - -0.8 2,525,315 + -1.1 2,525,315 + +0.5 2,525,315 + -0.3 2,525,316 - +0.0 2,525,316 - -1.7 2,525,316 - +0.0 2,525,316 - -2.1 2,525,369 + +0.9 2,525,385 - Pf1N1B4_2211 0.11 -2.1 2,525,405 + Pf1N1B4_2211 0.14 -0.1 2,525,405 + Pf1N1B4_2211 0.14 +1.7 2,525,567 + Pf1N1B4_2211 0.39 +0.5 2,525,568 - Pf1N1B4_2211 0.39 +0.5 2,525,568 - Pf1N1B4_2211 0.39 +0.7 2,525,666 + Pf1N1B4_2211 0.54 +0.9 2,525,667 - Pf1N1B4_2211 0.54 +0.6 2,525,667 - Pf1N1B4_2211 0.54 +2.0 2,525,798 + Pf1N1B4_2211 0.74 -0.5 2,525,798 + Pf1N1B4_2211 0.74 +0.5 2,525,798 + Pf1N1B4_2211 0.74 +1.5 2,525,798 + Pf1N1B4_2211 0.74 +0.9 2,525,798 + Pf1N1B4_2211 0.74 +1.5 2,525,799 - Pf1N1B4_2211 0.75 -0.5 2,525,799 - Pf1N1B4_2211 0.75 -0.1 2,525,799 - Pf1N1B4_2211 0.75 +0.5 2,525,799 - Pf1N1B4_2211 0.75 -0.5 2,525,880 + Pf1N1B4_2211 0.87 -0.5 2,525,982 + -0.5 2,525,982 + -1.1 2,525,982 + -1.1 2,525,982 + +0.5 2,525,982 + -3.1 2,525,982 + +0.0 2,525,982 + -0.1 2,525,982 + -0.1 2,525,982 + +1.5 2,525,982 + +0.8 2,525,982 + -0.1 2,525,982 + +1.5 2,525,982 + -2.4 2,525,982 + -1.1 2,525,982 + +0.1 2,525,982 + -0.0 2,525,982 + +1.5 2,525,982 + -0.1 2,525,983 - -0.5 2,525,983 - +0.1 2,525,983 - +0.0 2,525,983 - +0.3 2,525,983 - +0.4 2,525,983 - -2.4 2,525,983 - -0.4 2,525,983 - -0.1 2,525,983 - -0.8 2,525,983 - -1.1 2,525,986 + -0.1 2,525,987 - +0.0 2,525,987 - +0.9 2,525,987 - -1.5 2,525,987 - +0.4 2,525,987 - -0.1 2,525,987 - -1.0 2,526,040 + +0.5 2,526,040 + Pf1N1B4_2212 0.12 -0.5 2,526,040 + Pf1N1B4_2212 0.12 -0.5 2,526,040 + Pf1N1B4_2212 0.12 -2.1 2,526,040 + Pf1N1B4_2212 0.12 +1.0 2,526,041 - Pf1N1B4_2212 0.12 -1.9 2,526,041 - Pf1N1B4_2212 0.12 +1.1 2,526,041 - Pf1N1B4_2212 0.12 +0.2 2,526,041 - Pf1N1B4_2212 0.12 -0.9 2,526,041 - Pf1N1B4_2212 0.12 -1.0 2,526,041 - Pf1N1B4_2212 0.12 -1.1 2,526,061 + Pf1N1B4_2212 0.15 +0.9 2,526,061 + Pf1N1B4_2212 0.15 +0.3 2,526,061 + Pf1N1B4_2212 0.15 -1.4 2,526,061 + Pf1N1B4_2212 0.15 +0.0 2,526,061 + Pf1N1B4_2212 0.15 -1.1 2,526,061 + Pf1N1B4_2212 0.15 +0.1 2,526,062 - Pf1N1B4_2212 0.15 +1.2 2,526,062 - Pf1N1B4_2212 0.15 +1.8 2,526,062 - Pf1N1B4_2212 0.15 -0.3 2,526,084 - Pf1N1B4_2212 0.18 +1.5 2,526,084 - Pf1N1B4_2212 0.18 -0.1 2,526,323 - Pf1N1B4_2212 0.54 -0.3 2,526,323 - Pf1N1B4_2212 0.54 +1.8 2,526,393 + Pf1N1B4_2212 0.64 -0.5 2,526,454 + Pf1N1B4_2212 0.73 +0.3 2,526,455 - Pf1N1B4_2212 0.74 +0.5 2,526,455 - Pf1N1B4_2212 0.74 -0.1 2,526,625 + -2.7 2,526,625 + -1.4 2,526,626 - +0.0 2,526,632 + -1.4 2,526,632 + -2.1 2,526,632 + -0.0 2,526,632 + +0.1 2,526,633 - -0.7 2,526,633 - +0.1 2,526,633 - -1.4 2,526,633 - +1.0 2,526,633 - +0.5 2,526,785 + Pf1N1B4_2213 0.17 +0.3 2,526,786 - Pf1N1B4_2213 0.17 +1.5 2,526,786 - Pf1N1B4_2213 0.17 -0.5 2,526,786 - Pf1N1B4_2213 0.17 +0.5 2,526,902 + Pf1N1B4_2213 0.32 -1.5 2,526,902 + Pf1N1B4_2213 0.32 -0.1 2,526,903 - Pf1N1B4_2213 0.32 +0.2
Or see this region's nucleotide sequence