Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_20 and Pf1N1B4_21 are separated by 48 nucleotides Pf1N1B4_21 and Pf1N1B4_22 are separated by 30 nucleotides Pf1N1B4_22 and Pf1N1B4_5160 are separated by 185 nucleotides Pf1N1B4_5160 and Pf1N1B4_5161 are separated by 82 nucleotides Pf1N1B4_5161 and Pf1N1B4_5162 are separated by 56 nucleotides Pf1N1B4_5162 and Pf1N1B4_5163 are separated by 82 nucleotides Pf1N1B4_5163 and Pf1N1B4_23 are separated by 190 nucleotides
Pf1N1B4_20 - FIG002571: 4-hydroxybenzoyl-CoA thioesterase domain protein, at 23,652 to 24,101
_20
Pf1N1B4_21 - Probable hydrolase, at 24,150 to 24,983
_21
Pf1N1B4_22 - Transcriptional regulator, TetR family, at 25,014 to 25,553
_22
Pf1N1B4_5160 - tRNA-Glu-TTC, at 25,739 to 25,811
_5160
Pf1N1B4_5161 - tRNA-Ala-GGC, at 25,894 to 25,966
_5161
Pf1N1B4_5162 - tRNA-Glu-TTC, at 26,023 to 26,095
_5162
Pf1N1B4_5163 - tRNA-Ala-GGC, at 26,178 to 26,250
_5163
Pf1N1B4_23 - Glutamyl-tRNA synthetase (EC 6.1.1.17), at 26,441 to 26,560
_23
Pf1N1B4_24 - Glutamyl-tRNA synthetase (EC 6.1.1.17), at 26,536 to 27,849
_24
Position (kb) 25
26 Strain fitness (log2 ratio) -2
-1
0
1 at 24.121 kb on + strand at 24.122 kb on - strand at 24.122 kb on - strand at 24.122 kb on - strand at 24.252 kb on + strand, within Pf1N1B4_21 at 24.253 kb on - strand, within Pf1N1B4_21 at 24.365 kb on - strand, within Pf1N1B4_21 at 24.423 kb on - strand, within Pf1N1B4_21 at 24.423 kb on - strand at 24.458 kb on - strand, within Pf1N1B4_21 at 24.458 kb on - strand, within Pf1N1B4_21 at 24.458 kb on - strand, within Pf1N1B4_21 at 24.458 kb on - strand, within Pf1N1B4_21 at 24.478 kb on - strand, within Pf1N1B4_21 at 24.775 kb on - strand, within Pf1N1B4_21 at 25.285 kb on + strand, within Pf1N1B4_22 at 25.286 kb on - strand, within Pf1N1B4_22 at 25.286 kb on - strand, within Pf1N1B4_22 at 25.368 kb on + strand, within Pf1N1B4_22 at 25.369 kb on - strand, within Pf1N1B4_22 at 25.369 kb on - strand, within Pf1N1B4_22 at 25.369 kb on - strand, within Pf1N1B4_22 at 25.420 kb on + strand, within Pf1N1B4_22 at 25.420 kb on + strand, within Pf1N1B4_22 at 25.421 kb on - strand, within Pf1N1B4_22 at 25.421 kb on - strand, within Pf1N1B4_22 at 25.421 kb on - strand, within Pf1N1B4_22 at 25.421 kb on - strand at 26.171 kb on - strand at 26.185 kb on - strand at 26.185 kb on - strand at 26.185 kb on - strand at 26.339 kb on - strand at 26.339 kb on - strand at 26.350 kb on - strand at 26.423 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 24,121 + +0.7 24,122 - -0.3 24,122 - -1.6 24,122 - +0.7 24,252 + Pf1N1B4_21 0.12 -0.5 24,253 - Pf1N1B4_21 0.12 -0.7 24,365 - Pf1N1B4_21 0.26 -1.6 24,423 - Pf1N1B4_21 0.33 -0.7 24,423 - -2.6 24,458 - Pf1N1B4_21 0.37 -0.3 24,458 - Pf1N1B4_21 0.37 +1.3 24,458 - Pf1N1B4_21 0.37 +0.7 24,458 - Pf1N1B4_21 0.37 -0.3 24,478 - Pf1N1B4_21 0.39 -1.3 24,775 - Pf1N1B4_21 0.75 -2.3 25,285 + Pf1N1B4_22 0.50 -1.3 25,286 - Pf1N1B4_22 0.50 -1.3 25,286 - Pf1N1B4_22 0.50 -0.8 25,368 + Pf1N1B4_22 0.66 -2.3 25,369 - Pf1N1B4_22 0.66 -0.9 25,369 - Pf1N1B4_22 0.66 -1.6 25,369 - Pf1N1B4_22 0.66 -0.9 25,420 + Pf1N1B4_22 0.75 -1.1 25,420 + Pf1N1B4_22 0.75 -0.9 25,421 - Pf1N1B4_22 0.75 -0.3 25,421 - Pf1N1B4_22 0.75 -2.4 25,421 - Pf1N1B4_22 0.75 -0.1 25,421 - -0.3 26,171 - -0.9 26,185 - -0.9 26,185 - -2.3 26,185 - -0.3 26,339 - -0.3 26,339 - -0.0 26,350 - +1.0 26,423 - -1.3
Or see this region's nucleotide sequence