Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2174 and Pf1N1B4_2175 are separated by 9 nucleotides Pf1N1B4_2175 and Pf1N1B4_2176 are separated by 329 nucleotides Pf1N1B4_2176 and Pf1N1B4_2177 are separated by 4 nucleotides Pf1N1B4_2177 and Pf1N1B4_2178 are separated by 269 nucleotides Pf1N1B4_2178 and Pf1N1B4_2179 are separated by 7 nucleotides
Pf1N1B4_2174 - Diaminopimelate decarboxylase (EC 4.1.1.20), at 2,486,795 to 2,488,042
_2174
Pf1N1B4_2175 - Lipoprotein LppL-related protein, at 2,488,052 to 2,488,231
_2175
Pf1N1B4_2176 - Frataxin homolog CyaY, facilitates iron supply for heme A synthesis or Fe-S cluster assembly, at 2,488,561 to 2,488,893
_2176
Pf1N1B4_2177 - FIG00953342: hypothetical protein, at 2,488,898 to 2,489,116
_2177
Pf1N1B4_2178 - Regulator of nucleoside diphosphate kinase, at 2,489,386 to 2,489,796
_2178
Pf1N1B4_2179 - Adenylate cyclase (EC 4.6.1.1), at 2,489,804 to 2,492,650
_2179
Position (kb)
2488
2489
2490 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2488.031 kb on - strand at 2488.054 kb on - strand at 2488.057 kb on - strand at 2488.057 kb on - strand at 2488.057 kb on - strand at 2488.057 kb on - strand at 2488.057 kb on - strand at 2488.255 kb on - strand at 2488.269 kb on - strand at 2488.269 kb on - strand at 2488.269 kb on - strand at 2488.269 kb on - strand at 2488.293 kb on + strand at 2488.294 kb on - strand at 2488.342 kb on + strand at 2488.342 kb on + strand at 2488.342 kb on + strand at 2488.342 kb on + strand at 2488.342 kb on + strand at 2488.342 kb on + strand at 2488.342 kb on + strand at 2488.342 kb on + strand at 2488.343 kb on - strand at 2488.343 kb on - strand at 2488.343 kb on - strand at 2488.448 kb on + strand at 2488.448 kb on + strand at 2488.448 kb on + strand at 2488.448 kb on + strand at 2488.448 kb on + strand at 2488.457 kb on - strand at 2488.513 kb on + strand at 2488.514 kb on - strand at 2488.537 kb on + strand at 2488.537 kb on + strand at 2488.538 kb on - strand at 2488.597 kb on + strand, within Pf1N1B4_2176 at 2488.623 kb on + strand, within Pf1N1B4_2176 at 2488.623 kb on + strand, within Pf1N1B4_2176 at 2488.777 kb on + strand, within Pf1N1B4_2176 at 2488.778 kb on - strand at 2488.778 kb on - strand, within Pf1N1B4_2176 at 2488.937 kb on + strand, within Pf1N1B4_2177 at 2489.115 kb on - strand at 2489.236 kb on + strand at 2489.237 kb on - strand at 2489.237 kb on - strand at 2489.237 kb on - strand at 2489.264 kb on + strand at 2489.264 kb on + strand at 2489.264 kb on + strand at 2489.264 kb on + strand at 2489.265 kb on - strand at 2489.265 kb on - strand at 2489.265 kb on - strand at 2489.265 kb on - strand at 2489.265 kb on - strand at 2489.266 kb on + strand at 2489.266 kb on + strand at 2489.266 kb on + strand at 2489.266 kb on + strand at 2489.266 kb on + strand at 2489.266 kb on + strand at 2489.266 kb on + strand at 2489.266 kb on + strand at 2489.267 kb on - strand at 2489.267 kb on - strand at 2489.267 kb on - strand at 2489.267 kb on - strand at 2489.267 kb on - strand at 2489.270 kb on + strand at 2489.270 kb on + strand at 2489.270 kb on + strand at 2489.270 kb on + strand at 2489.270 kb on + strand at 2489.270 kb on + strand at 2489.271 kb on - strand at 2489.271 kb on - strand at 2489.271 kb on - strand at 2489.283 kb on + strand at 2489.596 kb on + strand, within Pf1N1B4_2178 at 2489.596 kb on + strand, within Pf1N1B4_2178 at 2489.596 kb on + strand, within Pf1N1B4_2178 at 2489.597 kb on - strand, within Pf1N1B4_2178 at 2489.785 kb on + strand at 2489.785 kb on + strand at 2489.786 kb on - strand at 2489.786 kb on - strand at 2489.854 kb on - strand at 2489.862 kb on + strand at 2489.862 kb on + strand at 2489.862 kb on + strand at 2489.862 kb on + strand at 2489.862 kb on + strand at 2489.862 kb on + strand at 2489.862 kb on + strand at 2489.863 kb on - strand at 2489.863 kb on - strand at 2489.863 kb on - strand at 2489.922 kb on + strand at 2489.922 kb on + strand at 2489.922 kb on + strand at 2489.922 kb on + strand at 2489.922 kb on + strand at 2489.922 kb on + strand at 2489.923 kb on - strand at 2490.030 kb on + strand at 2490.030 kb on + strand at 2490.031 kb on - strand at 2490.031 kb on - strand at 2490.031 kb on - strand at 2490.031 kb on - strand at 2490.031 kb on - strand at 2490.087 kb on + strand at 2490.087 kb on + strand at 2490.087 kb on + strand at 2490.087 kb on + strand at 2490.087 kb on + strand at 2490.087 kb on + strand at 2490.087 kb on + strand at 2490.088 kb on - strand at 2490.088 kb on - strand at 2490.088 kb on - strand at 2490.088 kb on - strand at 2490.088 kb on - strand at 2490.088 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 2,488,031 - -0.1 2,488,054 - +2.0 2,488,057 - -1.1 2,488,057 - -0.8 2,488,057 - +1.5 2,488,057 - -0.5 2,488,057 - -0.5 2,488,255 - -0.9 2,488,269 - +1.0 2,488,269 - -0.8 2,488,269 - +0.5 2,488,269 - +0.5 2,488,293 + +0.2 2,488,294 - +0.1 2,488,342 + -0.1 2,488,342 + +0.3 2,488,342 + -1.1 2,488,342 + -1.1 2,488,342 + -0.6 2,488,342 + +1.8 2,488,342 + +1.5 2,488,342 + -1.7 2,488,343 - +0.5 2,488,343 - -1.1 2,488,343 - +0.0 2,488,448 + -0.5 2,488,448 + +0.8 2,488,448 + +0.5 2,488,448 + -0.5 2,488,448 + +0.0 2,488,457 - -0.1 2,488,513 + -1.9 2,488,514 - -1.1 2,488,537 + -0.5 2,488,537 + +0.0 2,488,538 - -1.2 2,488,597 + Pf1N1B4_2176 0.11 -1.9 2,488,623 + Pf1N1B4_2176 0.19 -2.5 2,488,623 + Pf1N1B4_2176 0.19 -0.3 2,488,777 + Pf1N1B4_2176 0.65 -0.5 2,488,778 - -0.5 2,488,778 - Pf1N1B4_2176 0.65 -2.0 2,488,937 + Pf1N1B4_2177 0.18 +1.0 2,489,115 - +0.0 2,489,236 + -0.5 2,489,237 - +0.0 2,489,237 - +1.1 2,489,237 - -0.0 2,489,264 + -1.1 2,489,264 + -1.9 2,489,264 + +0.8 2,489,264 + +0.5 2,489,265 - +0.9 2,489,265 - -0.3 2,489,265 - +0.5 2,489,265 - +0.1 2,489,265 - -1.1 2,489,266 + +0.0 2,489,266 + +0.5 2,489,266 + -0.7 2,489,266 + +0.1 2,489,266 + -1.9 2,489,266 + -1.1 2,489,266 + +0.5 2,489,266 + +0.5 2,489,267 - +0.2 2,489,267 - +2.0 2,489,267 - +1.5 2,489,267 - -1.4 2,489,267 - -0.5 2,489,270 + -1.0 2,489,270 + -2.4 2,489,270 + +2.5 2,489,270 + -0.9 2,489,270 + +1.7 2,489,270 + +1.5 2,489,271 - +1.5 2,489,271 - -1.8 2,489,271 - -1.5 2,489,283 + +1.2 2,489,596 + Pf1N1B4_2178 0.51 +1.5 2,489,596 + Pf1N1B4_2178 0.51 +0.5 2,489,596 + Pf1N1B4_2178 0.51 +0.6 2,489,597 - Pf1N1B4_2178 0.51 -1.1 2,489,785 + +0.5 2,489,785 + +0.5 2,489,786 - -1.1 2,489,786 - -0.5 2,489,854 - -0.1 2,489,862 + +2.0 2,489,862 + +0.5 2,489,862 + -1.1 2,489,862 + -1.1 2,489,862 + -0.1 2,489,862 + +0.1 2,489,862 + -0.9 2,489,863 - +0.1 2,489,863 - +0.5 2,489,863 - -0.1 2,489,922 + -0.5 2,489,922 + -0.1 2,489,922 + -1.0 2,489,922 + +0.5 2,489,922 + +1.5 2,489,922 + -0.8 2,489,923 - +0.1 2,490,030 + +0.5 2,490,030 + -2.5 2,490,031 - +1.3 2,490,031 - -0.2 2,490,031 - +1.0 2,490,031 - -2.2 2,490,031 - -0.5 2,490,087 + +0.0 2,490,087 + +0.5 2,490,087 + -1.5 2,490,087 + +0.9 2,490,087 + +0.5 2,490,087 + +0.1 2,490,087 + -0.1 2,490,088 - -1.1 2,490,088 - +1.0 2,490,088 - -1.9 2,490,088 - -1.5 2,490,088 - -1.1 2,490,088 - -0.4
Or see this region's nucleotide sequence