Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2167 and Pf1N1B4_2168 are separated by 214 nucleotides Pf1N1B4_2168 and Pf1N1B4_2169 are separated by 104 nucleotides Pf1N1B4_2169 and Pf1N1B4_2170 are separated by 162 nucleotides Pf1N1B4_2170 and Pf1N1B4_2171 overlap by 4 nucleotides
Pf1N1B4_2167 - Ammonium transporter, at 2,481,053 to 2,482,390
_2167
Pf1N1B4_2168 - FIG004064: hypothetical protein, at 2,482,605 to 2,483,030
_2168
Pf1N1B4_2169 - FIG00953808: hypothetical protein, at 2,483,135 to 2,483,458
_2169
Pf1N1B4_2170 - Putative FMN hydrolase (EC 3.1.3.-); 5-Amino-6-(5'-phosphoribitylamino)uracil phosphatase, at 2,483,621 to 2,484,322
_2170
Pf1N1B4_2171 - Tyrosine recombinase XerC, at 2,484,319 to 2,485,218
_2171
Position (kb)
2483
2484 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2482.262 kb on + strand at 2482.262 kb on + strand at 2482.262 kb on + strand at 2482.262 kb on + strand at 2482.262 kb on + strand at 2482.263 kb on - strand at 2482.263 kb on - strand at 2482.263 kb on - strand at 2482.282 kb on + strand at 2482.321 kb on + strand at 2482.388 kb on + strand at 2482.388 kb on + strand at 2482.388 kb on + strand at 2482.388 kb on + strand at 2482.389 kb on - strand at 2482.389 kb on - strand at 2482.389 kb on - strand at 2482.389 kb on - strand at 2482.389 kb on - strand at 2482.389 kb on - strand at 2482.527 kb on - strand at 2482.527 kb on - strand at 2482.542 kb on + strand at 2482.542 kb on + strand at 2482.543 kb on - strand at 2482.543 kb on - strand at 2482.543 kb on - strand at 2482.543 kb on - strand at 2482.543 kb on - strand at 2482.543 kb on - strand at 2482.574 kb on + strand at 2482.574 kb on + strand at 2482.604 kb on + strand at 2482.604 kb on + strand at 2482.604 kb on + strand at 2482.604 kb on + strand at 2482.605 kb on - strand at 2482.605 kb on - strand at 2482.605 kb on - strand at 2482.605 kb on - strand at 2482.605 kb on - strand at 2482.605 kb on - strand at 2482.605 kb on - strand at 2482.605 kb on - strand at 2482.605 kb on - strand at 2482.605 kb on - strand at 2482.605 kb on - strand at 2482.605 kb on - strand at 2482.605 kb on - strand at 2482.605 kb on - strand at 2482.605 kb on - strand at 2482.605 kb on - strand at 2482.605 kb on - strand at 2482.605 kb on - strand at 2482.605 kb on - strand at 2482.605 kb on - strand at 2482.605 kb on - strand at 2482.605 kb on - strand at 2482.673 kb on - strand, within Pf1N1B4_2168 at 2482.673 kb on - strand, within Pf1N1B4_2168 at 2482.673 kb on - strand, within Pf1N1B4_2168 at 2482.673 kb on - strand, within Pf1N1B4_2168 at 2482.673 kb on - strand, within Pf1N1B4_2168 at 2482.729 kb on + strand, within Pf1N1B4_2168 at 2482.736 kb on + strand, within Pf1N1B4_2168 at 2482.737 kb on - strand, within Pf1N1B4_2168 at 2482.737 kb on - strand, within Pf1N1B4_2168 at 2482.737 kb on - strand, within Pf1N1B4_2168 at 2482.737 kb on - strand, within Pf1N1B4_2168 at 2482.737 kb on - strand, within Pf1N1B4_2168 at 2482.737 kb on - strand, within Pf1N1B4_2168 at 2482.737 kb on - strand, within Pf1N1B4_2168 at 2482.737 kb on - strand, within Pf1N1B4_2168 at 2482.737 kb on - strand, within Pf1N1B4_2168 at 2482.737 kb on - strand, within Pf1N1B4_2168 at 2482.737 kb on - strand, within Pf1N1B4_2168 at 2482.737 kb on - strand, within Pf1N1B4_2168 at 2482.737 kb on - strand, within Pf1N1B4_2168 at 2482.781 kb on - strand at 2482.781 kb on - strand, within Pf1N1B4_2168 at 2482.837 kb on - strand, within Pf1N1B4_2168 at 2482.845 kb on - strand, within Pf1N1B4_2168 at 2482.845 kb on - strand, within Pf1N1B4_2168 at 2482.891 kb on + strand, within Pf1N1B4_2168 at 2482.891 kb on + strand, within Pf1N1B4_2168 at 2482.891 kb on + strand at 2482.892 kb on - strand at 2482.892 kb on - strand, within Pf1N1B4_2168 at 2482.892 kb on - strand, within Pf1N1B4_2168 at 2482.892 kb on - strand, within Pf1N1B4_2168 at 2482.892 kb on - strand, within Pf1N1B4_2168 at 2482.892 kb on - strand, within Pf1N1B4_2168 at 2482.892 kb on - strand, within Pf1N1B4_2168 at 2482.892 kb on - strand, within Pf1N1B4_2168 at 2482.959 kb on + strand, within Pf1N1B4_2168 at 2482.960 kb on - strand, within Pf1N1B4_2168 at 2482.960 kb on - strand, within Pf1N1B4_2168 at 2482.960 kb on - strand, within Pf1N1B4_2168 at 2482.960 kb on - strand, within Pf1N1B4_2168 at 2482.960 kb on - strand, within Pf1N1B4_2168 at 2482.995 kb on - strand at 2483.098 kb on - strand at 2483.099 kb on + strand at 2483.099 kb on + strand at 2483.099 kb on + strand at 2483.099 kb on + strand at 2483.099 kb on + strand at 2483.100 kb on - strand at 2483.100 kb on - strand at 2483.100 kb on - strand at 2483.100 kb on - strand at 2483.100 kb on - strand at 2483.100 kb on - strand at 2483.100 kb on - strand at 2483.100 kb on - strand at 2483.100 kb on - strand at 2483.100 kb on - strand at 2483.100 kb on - strand at 2483.100 kb on - strand at 2483.103 kb on + strand at 2483.103 kb on + strand at 2483.103 kb on + strand at 2483.103 kb on + strand at 2483.103 kb on + strand at 2483.103 kb on + strand at 2483.104 kb on - strand at 2483.104 kb on - strand at 2483.104 kb on - strand at 2483.104 kb on - strand at 2483.104 kb on - strand at 2483.104 kb on - strand at 2483.104 kb on - strand at 2483.104 kb on - strand at 2483.104 kb on - strand at 2483.104 kb on - strand at 2483.104 kb on - strand at 2483.129 kb on + strand at 2483.129 kb on + strand at 2483.129 kb on + strand at 2483.129 kb on + strand at 2483.129 kb on + strand at 2483.129 kb on + strand at 2483.129 kb on + strand at 2483.129 kb on + strand at 2483.129 kb on + strand at 2483.129 kb on + strand at 2483.129 kb on + strand at 2483.129 kb on + strand at 2483.130 kb on - strand at 2483.130 kb on - strand at 2483.130 kb on - strand at 2483.130 kb on - strand at 2483.130 kb on - strand at 2483.130 kb on - strand at 2483.130 kb on - strand at 2483.130 kb on - strand at 2483.130 kb on - strand at 2483.172 kb on + strand, within Pf1N1B4_2169 at 2483.172 kb on + strand, within Pf1N1B4_2169 at 2483.172 kb on + strand, within Pf1N1B4_2169 at 2483.172 kb on + strand, within Pf1N1B4_2169 at 2483.172 kb on + strand, within Pf1N1B4_2169 at 2483.173 kb on - strand, within Pf1N1B4_2169 at 2483.173 kb on - strand, within Pf1N1B4_2169 at 2483.173 kb on - strand, within Pf1N1B4_2169 at 2483.173 kb on - strand, within Pf1N1B4_2169 at 2483.432 kb on - strand at 2483.438 kb on + strand at 2483.439 kb on - strand at 2483.439 kb on - strand at 2483.439 kb on - strand at 2483.452 kb on + strand at 2483.452 kb on + strand at 2483.452 kb on + strand at 2483.452 kb on + strand at 2483.453 kb on - strand at 2483.453 kb on - strand at 2483.453 kb on - strand at 2483.453 kb on - strand at 2483.456 kb on + strand at 2483.456 kb on + strand at 2483.456 kb on + strand at 2483.456 kb on + strand at 2483.456 kb on + strand at 2483.456 kb on + strand at 2483.456 kb on + strand at 2483.456 kb on + strand at 2483.456 kb on + strand at 2483.456 kb on + strand at 2483.456 kb on + strand at 2483.456 kb on + strand at 2483.457 kb on - strand at 2483.457 kb on - strand at 2483.457 kb on - strand at 2483.457 kb on - strand at 2483.457 kb on - strand at 2483.457 kb on - strand at 2483.457 kb on - strand at 2483.457 kb on - strand at 2483.457 kb on - strand at 2483.457 kb on - strand at 2483.457 kb on - strand at 2483.553 kb on + strand at 2483.553 kb on + strand at 2483.553 kb on + strand at 2483.553 kb on + strand at 2483.554 kb on - strand at 2483.554 kb on - strand at 2483.554 kb on - strand at 2483.554 kb on - strand at 2483.554 kb on - strand at 2483.554 kb on - strand at 2483.554 kb on - strand at 2483.558 kb on + strand at 2483.558 kb on + strand at 2483.558 kb on + strand at 2483.559 kb on - strand at 2483.559 kb on - strand at 2483.569 kb on + strand at 2483.569 kb on + strand at 2483.569 kb on + strand at 2483.570 kb on - strand at 2483.570 kb on - strand at 2483.570 kb on - strand at 2483.570 kb on - strand at 2483.570 kb on - strand at 2483.570 kb on - strand at 2483.570 kb on - strand at 2483.577 kb on + strand at 2483.578 kb on - strand at 2483.623 kb on - strand at 2483.623 kb on - strand at 2483.623 kb on - strand at 2483.623 kb on - strand at 2483.629 kb on + strand at 2483.629 kb on + strand at 2483.630 kb on - strand at 2483.630 kb on - strand at 2483.668 kb on + strand at 2483.705 kb on + strand, within Pf1N1B4_2170 at 2483.706 kb on - strand, within Pf1N1B4_2170 at 2483.788 kb on + strand, within Pf1N1B4_2170 at 2483.789 kb on - strand, within Pf1N1B4_2170 at 2483.789 kb on - strand, within Pf1N1B4_2170 at 2483.789 kb on - strand, within Pf1N1B4_2170 at 2483.789 kb on - strand, within Pf1N1B4_2170 at 2483.899 kb on - strand, within Pf1N1B4_2170 at 2483.951 kb on + strand, within Pf1N1B4_2170 at 2483.952 kb on - strand, within Pf1N1B4_2170 at 2483.952 kb on - strand, within Pf1N1B4_2170 at 2483.958 kb on + strand, within Pf1N1B4_2170 at 2483.958 kb on + strand, within Pf1N1B4_2170 at 2484.070 kb on + strand, within Pf1N1B4_2170 at 2484.070 kb on + strand, within Pf1N1B4_2170 at 2484.071 kb on - strand, within Pf1N1B4_2170 at 2484.071 kb on - strand, within Pf1N1B4_2170 at 2484.071 kb on - strand, within Pf1N1B4_2170 at 2484.071 kb on - strand, within Pf1N1B4_2170 at 2484.071 kb on - strand, within Pf1N1B4_2170 at 2484.336 kb on + strand at 2484.368 kb on + strand at 2484.368 kb on + strand at 2484.368 kb on + strand at 2484.404 kb on + strand at 2484.404 kb on + strand at 2484.404 kb on + strand at 2484.404 kb on + strand at 2484.404 kb on + strand at 2484.405 kb on - strand at 2484.405 kb on - strand at 2484.405 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 2,482,262 + +0.1 2,482,262 + +0.5 2,482,262 + -0.0 2,482,262 + +0.5 2,482,262 + +0.5 2,482,263 - -0.8 2,482,263 - -0.5 2,482,263 - +0.5 2,482,282 + -0.3 2,482,321 + -0.3 2,482,388 + -0.7 2,482,388 + +0.3 2,482,388 + -0.9 2,482,388 + -2.1 2,482,389 - -0.5 2,482,389 - -1.1 2,482,389 - +1.3 2,482,389 - -0.5 2,482,389 - -0.2 2,482,389 - +0.2 2,482,527 - +0.1 2,482,527 - -0.8 2,482,542 + +1.0 2,482,542 + -0.5 2,482,543 - -1.9 2,482,543 - -0.1 2,482,543 - -0.1 2,482,543 - -0.1 2,482,543 - +0.0 2,482,543 - +0.5 2,482,574 + -2.3 2,482,574 + -0.1 2,482,604 + -1.9 2,482,604 + -0.9 2,482,604 + +1.5 2,482,604 + -1.5 2,482,605 - +0.0 2,482,605 - +0.0 2,482,605 - +0.9 2,482,605 - -0.8 2,482,605 - -0.5 2,482,605 - +0.1 2,482,605 - +2.8 2,482,605 - -0.1 2,482,605 - -1.5 2,482,605 - +1.5 2,482,605 - -0.2 2,482,605 - -1.7 2,482,605 - -1.1 2,482,605 - -1.1 2,482,605 - -1.3 2,482,605 - +0.1 2,482,605 - +1.0 2,482,605 - -0.5 2,482,605 - +0.5 2,482,605 - +0.5 2,482,605 - +0.0 2,482,605 - -0.1 2,482,673 - Pf1N1B4_2168 0.16 +0.5 2,482,673 - Pf1N1B4_2168 0.16 -1.2 2,482,673 - Pf1N1B4_2168 0.16 -0.5 2,482,673 - Pf1N1B4_2168 0.16 +0.1 2,482,673 - Pf1N1B4_2168 0.16 -1.9 2,482,729 + Pf1N1B4_2168 0.29 +0.5 2,482,736 + Pf1N1B4_2168 0.31 -2.3 2,482,737 - Pf1N1B4_2168 0.31 -0.2 2,482,737 - Pf1N1B4_2168 0.31 -1.3 2,482,737 - Pf1N1B4_2168 0.31 +0.5 2,482,737 - Pf1N1B4_2168 0.31 -1.9 2,482,737 - Pf1N1B4_2168 0.31 -1.1 2,482,737 - Pf1N1B4_2168 0.31 -1.1 2,482,737 - Pf1N1B4_2168 0.31 -0.5 2,482,737 - Pf1N1B4_2168 0.31 -2.1 2,482,737 - Pf1N1B4_2168 0.31 -1.3 2,482,737 - Pf1N1B4_2168 0.31 +0.8 2,482,737 - Pf1N1B4_2168 0.31 +0.9 2,482,737 - Pf1N1B4_2168 0.31 +0.7 2,482,737 - Pf1N1B4_2168 0.31 -1.9 2,482,781 - -0.5 2,482,781 - Pf1N1B4_2168 0.41 -1.9 2,482,837 - Pf1N1B4_2168 0.54 +0.7 2,482,845 - Pf1N1B4_2168 0.56 +0.5 2,482,845 - Pf1N1B4_2168 0.56 +1.5 2,482,891 + Pf1N1B4_2168 0.67 +0.5 2,482,891 + Pf1N1B4_2168 0.67 -2.3 2,482,891 + -1.5 2,482,892 - -0.5 2,482,892 - Pf1N1B4_2168 0.67 +0.5 2,482,892 - Pf1N1B4_2168 0.67 -0.5 2,482,892 - Pf1N1B4_2168 0.67 -0.9 2,482,892 - Pf1N1B4_2168 0.67 -0.1 2,482,892 - Pf1N1B4_2168 0.67 -2.0 2,482,892 - Pf1N1B4_2168 0.67 -0.7 2,482,892 - Pf1N1B4_2168 0.67 -0.5 2,482,959 + Pf1N1B4_2168 0.83 -1.6 2,482,960 - Pf1N1B4_2168 0.83 -0.3 2,482,960 - Pf1N1B4_2168 0.83 -0.3 2,482,960 - Pf1N1B4_2168 0.83 +0.9 2,482,960 - Pf1N1B4_2168 0.83 -1.1 2,482,960 - Pf1N1B4_2168 0.83 -0.1 2,482,995 - +1.5 2,483,098 - +0.0 2,483,099 + -0.5 2,483,099 + -0.5 2,483,099 + -1.9 2,483,099 + +1.5 2,483,099 + -1.3 2,483,100 - +1.5 2,483,100 - +1.2 2,483,100 - -1.5 2,483,100 - +0.9 2,483,100 - +0.1 2,483,100 - -1.0 2,483,100 - +0.0 2,483,100 - +0.5 2,483,100 - +0.5 2,483,100 - +0.9 2,483,100 - -0.8 2,483,100 - -1.0 2,483,103 + -1.9 2,483,103 + +0.5 2,483,103 + -2.3 2,483,103 + -0.2 2,483,103 + -1.5 2,483,103 + +1.7 2,483,104 - +0.5 2,483,104 - +1.5 2,483,104 - +0.5 2,483,104 - -0.9 2,483,104 - +0.5 2,483,104 - -1.1 2,483,104 - -0.8 2,483,104 - -1.1 2,483,104 - +0.5 2,483,104 - +0.5 2,483,104 - +1.0 2,483,129 + -1.3 2,483,129 + -0.5 2,483,129 + -1.0 2,483,129 + -1.5 2,483,129 + -1.3 2,483,129 + -0.1 2,483,129 + +0.5 2,483,129 + -1.1 2,483,129 + +1.5 2,483,129 + -1.0 2,483,129 + +0.5 2,483,129 + +0.5 2,483,130 - +1.5 2,483,130 - +1.5 2,483,130 - -2.1 2,483,130 - +0.0 2,483,130 - -1.1 2,483,130 - -1.7 2,483,130 - -0.5 2,483,130 - -0.5 2,483,130 - -0.5 2,483,172 + Pf1N1B4_2169 0.11 -0.3 2,483,172 + Pf1N1B4_2169 0.11 -0.5 2,483,172 + Pf1N1B4_2169 0.11 +2.5 2,483,172 + Pf1N1B4_2169 0.11 +0.5 2,483,172 + Pf1N1B4_2169 0.11 +0.9 2,483,173 - Pf1N1B4_2169 0.12 +1.5 2,483,173 - Pf1N1B4_2169 0.12 +3.0 2,483,173 - Pf1N1B4_2169 0.12 -1.5 2,483,173 - Pf1N1B4_2169 0.12 +2.0 2,483,432 - -0.5 2,483,438 + -0.9 2,483,439 - -0.3 2,483,439 - -0.1 2,483,439 - -0.7 2,483,452 + -0.2 2,483,452 + -1.1 2,483,452 + +1.5 2,483,452 + -1.0 2,483,453 - +0.5 2,483,453 - -0.1 2,483,453 - +0.9 2,483,453 - -0.5 2,483,456 + -1.0 2,483,456 + -0.5 2,483,456 + -0.1 2,483,456 + -1.9 2,483,456 + -0.0 2,483,456 + -0.5 2,483,456 + -1.0 2,483,456 + -0.5 2,483,456 + -1.1 2,483,456 + -0.5 2,483,456 + +0.9 2,483,456 + +0.2 2,483,457 - -0.5 2,483,457 - -1.7 2,483,457 - +1.0 2,483,457 - -0.5 2,483,457 - +0.9 2,483,457 - -1.5 2,483,457 - -0.3 2,483,457 - +0.5 2,483,457 - +0.5 2,483,457 - -0.1 2,483,457 - -0.5 2,483,553 + -0.3 2,483,553 + +1.0 2,483,553 + -1.5 2,483,553 + -0.5 2,483,554 - +0.2 2,483,554 - -0.1 2,483,554 - +1.0 2,483,554 - -0.9 2,483,554 - +0.2 2,483,554 - -0.5 2,483,554 - +0.5 2,483,558 + +0.1 2,483,558 + +0.5 2,483,558 + -0.1 2,483,559 - -1.9 2,483,559 - +1.5 2,483,569 + +0.1 2,483,569 + -0.9 2,483,569 + +2.3 2,483,570 - +0.1 2,483,570 - +0.5 2,483,570 - -0.3 2,483,570 - +0.5 2,483,570 - +0.8 2,483,570 - -1.9 2,483,570 - +0.5 2,483,577 + -0.8 2,483,578 - -0.4 2,483,623 - -0.3 2,483,623 - -1.7 2,483,623 - +0.2 2,483,623 - -1.5 2,483,629 + -1.7 2,483,629 + +0.7 2,483,630 - -1.7 2,483,630 - -1.9 2,483,668 + -0.8 2,483,705 + Pf1N1B4_2170 0.12 -0.2 2,483,706 - Pf1N1B4_2170 0.12 -1.1 2,483,788 + Pf1N1B4_2170 0.24 -0.5 2,483,789 - Pf1N1B4_2170 0.24 +1.5 2,483,789 - Pf1N1B4_2170 0.24 -0.1 2,483,789 - Pf1N1B4_2170 0.24 +0.5 2,483,789 - Pf1N1B4_2170 0.24 -1.5 2,483,899 - Pf1N1B4_2170 0.40 -0.3 2,483,951 + Pf1N1B4_2170 0.47 -1.7 2,483,952 - Pf1N1B4_2170 0.47 +0.5 2,483,952 - Pf1N1B4_2170 0.47 +0.5 2,483,958 + Pf1N1B4_2170 0.48 -1.9 2,483,958 + Pf1N1B4_2170 0.48 +0.5 2,484,070 + Pf1N1B4_2170 0.64 +0.5 2,484,070 + Pf1N1B4_2170 0.64 +0.2 2,484,071 - Pf1N1B4_2170 0.64 -0.7 2,484,071 - Pf1N1B4_2170 0.64 -0.3 2,484,071 - Pf1N1B4_2170 0.64 +0.5 2,484,071 - Pf1N1B4_2170 0.64 +0.0 2,484,071 - Pf1N1B4_2170 0.64 +0.1 2,484,336 + +1.5 2,484,368 + -0.1 2,484,368 + -0.1 2,484,368 + -0.4 2,484,404 + -0.1 2,484,404 + -2.9 2,484,404 + +0.1 2,484,404 + -0.5 2,484,404 + -1.1 2,484,405 - -0.9 2,484,405 - -0.5 2,484,405 - +0.5
Or see this region's nucleotide sequence