Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2070 and Pf1N1B4_2071 overlap by 4 nucleotides Pf1N1B4_2071 and Pf1N1B4_2072 are separated by 54 nucleotides Pf1N1B4_2072 and Pf1N1B4_2073 overlap by 4 nucleotides
Pf1N1B4_2070 - IcmF-related protein, at 2,363,058 to 2,366,558
_2070
Pf1N1B4_2071 - Protein phosphatase ImpM, at 2,366,555 to 2,367,205
_2071
Pf1N1B4_2072 - Phosphoprotein phosphatase PppA, at 2,367,260 to 2,367,937
_2072
Pf1N1B4_2073 - Serine/threonine protein kinase (EC 2.7.11.1) PpkA, at 2,367,934 to 2,370,978
_2073
Position (kb)
2366
2367
2368 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2365.671 kb on + strand, within Pf1N1B4_2070 at 2365.671 kb on + strand, within Pf1N1B4_2070 at 2365.671 kb on + strand, within Pf1N1B4_2070 at 2365.671 kb on + strand, within Pf1N1B4_2070 at 2365.671 kb on + strand, within Pf1N1B4_2070 at 2365.672 kb on - strand, within Pf1N1B4_2070 at 2365.672 kb on - strand, within Pf1N1B4_2070 at 2365.672 kb on - strand, within Pf1N1B4_2070 at 2365.672 kb on - strand, within Pf1N1B4_2070 at 2365.672 kb on - strand, within Pf1N1B4_2070 at 2365.672 kb on - strand, within Pf1N1B4_2070 at 2365.672 kb on - strand, within Pf1N1B4_2070 at 2366.004 kb on + strand, within Pf1N1B4_2070 at 2366.004 kb on + strand, within Pf1N1B4_2070 at 2366.005 kb on - strand, within Pf1N1B4_2070 at 2366.005 kb on - strand, within Pf1N1B4_2070 at 2366.005 kb on - strand, within Pf1N1B4_2070 at 2366.016 kb on + strand, within Pf1N1B4_2070 at 2366.017 kb on - strand, within Pf1N1B4_2070 at 2366.017 kb on - strand, within Pf1N1B4_2070 at 2366.017 kb on - strand at 2366.028 kb on + strand, within Pf1N1B4_2070 at 2366.029 kb on - strand at 2366.142 kb on + strand, within Pf1N1B4_2070 at 2366.142 kb on + strand, within Pf1N1B4_2070 at 2366.142 kb on + strand, within Pf1N1B4_2070 at 2366.143 kb on - strand, within Pf1N1B4_2070 at 2366.143 kb on - strand, within Pf1N1B4_2070 at 2366.143 kb on - strand, within Pf1N1B4_2070 at 2366.253 kb on + strand at 2366.253 kb on + strand at 2366.479 kb on - strand at 2366.479 kb on - strand at 2366.479 kb on - strand at 2366.525 kb on - strand at 2366.555 kb on - strand at 2366.706 kb on - strand, within Pf1N1B4_2071 at 2366.706 kb on - strand, within Pf1N1B4_2071 at 2366.982 kb on - strand, within Pf1N1B4_2071 at 2366.982 kb on - strand, within Pf1N1B4_2071 at 2366.982 kb on - strand, within Pf1N1B4_2071 at 2367.203 kb on + strand at 2367.203 kb on + strand at 2367.203 kb on + strand at 2367.203 kb on + strand at 2367.204 kb on - strand at 2367.204 kb on - strand at 2367.204 kb on - strand at 2367.204 kb on - strand at 2367.204 kb on - strand at 2367.204 kb on - strand at 2367.204 kb on - strand at 2367.204 kb on - strand at 2367.204 kb on - strand at 2367.259 kb on + strand at 2367.260 kb on - strand at 2367.356 kb on + strand, within Pf1N1B4_2072 at 2367.419 kb on + strand, within Pf1N1B4_2072 at 2367.420 kb on - strand, within Pf1N1B4_2072 at 2367.420 kb on - strand, within Pf1N1B4_2072 at 2367.576 kb on - strand, within Pf1N1B4_2072 at 2367.623 kb on + strand, within Pf1N1B4_2072 at 2367.623 kb on + strand, within Pf1N1B4_2072 at 2367.624 kb on - strand, within Pf1N1B4_2072 at 2367.624 kb on - strand, within Pf1N1B4_2072 at 2367.624 kb on - strand, within Pf1N1B4_2072 at 2367.624 kb on - strand, within Pf1N1B4_2072 at 2367.691 kb on + strand, within Pf1N1B4_2072 at 2367.770 kb on + strand, within Pf1N1B4_2072 at 2367.771 kb on - strand, within Pf1N1B4_2072 at 2367.951 kb on + strand at 2367.951 kb on + strand at 2367.952 kb on - strand at 2367.952 kb on - strand at 2367.952 kb on - strand at 2367.967 kb on + strand at 2368.012 kb on + strand at 2368.013 kb on - strand at 2368.013 kb on - strand at 2368.013 kb on - strand at 2368.117 kb on - strand at 2368.146 kb on + strand at 2368.146 kb on + strand at 2368.146 kb on + strand at 2368.147 kb on - strand at 2368.187 kb on - strand at 2368.187 kb on - strand at 2368.187 kb on - strand at 2368.187 kb on - strand at 2368.187 kb on - strand at 2368.189 kb on + strand at 2368.189 kb on + strand at 2368.189 kb on + strand at 2368.189 kb on + strand at 2368.189 kb on + strand at 2368.189 kb on + strand at 2368.189 kb on + strand at 2368.189 kb on + strand at 2368.189 kb on + strand at 2368.189 kb on + strand at 2368.189 kb on + strand at 2368.190 kb on - strand at 2368.190 kb on - strand at 2368.190 kb on - strand at 2368.190 kb on - strand at 2368.190 kb on - strand at 2368.190 kb on - strand at 2368.190 kb on - strand at 2368.190 kb on - strand at 2368.190 kb on - strand at 2368.190 kb on - strand at 2368.190 kb on - strand at 2368.190 kb on - strand at 2368.190 kb on - strand at 2368.190 kb on - strand at 2368.190 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 2,365,671 + Pf1N1B4_2070 0.75 -1.5 2,365,671 + Pf1N1B4_2070 0.75 +0.5 2,365,671 + Pf1N1B4_2070 0.75 -1.8 2,365,671 + Pf1N1B4_2070 0.75 -0.1 2,365,671 + Pf1N1B4_2070 0.75 +0.9 2,365,672 - Pf1N1B4_2070 0.75 -1.1 2,365,672 - Pf1N1B4_2070 0.75 -1.1 2,365,672 - Pf1N1B4_2070 0.75 +1.1 2,365,672 - Pf1N1B4_2070 0.75 -0.5 2,365,672 - Pf1N1B4_2070 0.75 +0.5 2,365,672 - Pf1N1B4_2070 0.75 +0.2 2,365,672 - Pf1N1B4_2070 0.75 -1.5 2,366,004 + Pf1N1B4_2070 0.84 +0.5 2,366,004 + Pf1N1B4_2070 0.84 -1.7 2,366,005 - Pf1N1B4_2070 0.84 -2.7 2,366,005 - Pf1N1B4_2070 0.84 -2.5 2,366,005 - Pf1N1B4_2070 0.84 +0.5 2,366,016 + Pf1N1B4_2070 0.84 -0.5 2,366,017 - Pf1N1B4_2070 0.85 +0.7 2,366,017 - Pf1N1B4_2070 0.85 +0.9 2,366,017 - +1.1 2,366,028 + Pf1N1B4_2070 0.85 +0.9 2,366,029 - -0.5 2,366,142 + Pf1N1B4_2070 0.88 -0.6 2,366,142 + Pf1N1B4_2070 0.88 +0.9 2,366,142 + Pf1N1B4_2070 0.88 +0.9 2,366,143 - Pf1N1B4_2070 0.88 -0.2 2,366,143 - Pf1N1B4_2070 0.88 +0.9 2,366,143 - Pf1N1B4_2070 0.88 -1.5 2,366,253 + +1.1 2,366,253 + -0.5 2,366,479 - -3.1 2,366,479 - -0.5 2,366,479 - -1.7 2,366,525 - -1.8 2,366,555 - -1.1 2,366,706 - Pf1N1B4_2071 0.23 -0.5 2,366,706 - Pf1N1B4_2071 0.23 +0.8 2,366,982 - Pf1N1B4_2071 0.66 +1.8 2,366,982 - Pf1N1B4_2071 0.66 +0.5 2,366,982 - Pf1N1B4_2071 0.66 -0.1 2,367,203 + -0.1 2,367,203 + -1.8 2,367,203 + -0.1 2,367,203 + -0.1 2,367,204 - +0.2 2,367,204 - +1.2 2,367,204 - +1.5 2,367,204 - -0.5 2,367,204 - +0.5 2,367,204 - -0.8 2,367,204 - -1.1 2,367,204 - -0.5 2,367,204 - -0.8 2,367,259 + -0.8 2,367,260 - -0.2 2,367,356 + Pf1N1B4_2072 0.14 +0.5 2,367,419 + Pf1N1B4_2072 0.23 -1.5 2,367,420 - Pf1N1B4_2072 0.24 +1.5 2,367,420 - Pf1N1B4_2072 0.24 +0.3 2,367,576 - Pf1N1B4_2072 0.47 +1.1 2,367,623 + Pf1N1B4_2072 0.54 +1.5 2,367,623 + Pf1N1B4_2072 0.54 +0.5 2,367,624 - Pf1N1B4_2072 0.54 +0.1 2,367,624 - Pf1N1B4_2072 0.54 +0.9 2,367,624 - Pf1N1B4_2072 0.54 -0.9 2,367,624 - Pf1N1B4_2072 0.54 -1.1 2,367,691 + Pf1N1B4_2072 0.64 -0.8 2,367,770 + Pf1N1B4_2072 0.75 -0.5 2,367,771 - Pf1N1B4_2072 0.75 +0.9 2,367,951 + +0.1 2,367,951 + +0.3 2,367,952 - +1.5 2,367,952 - -0.1 2,367,952 - -0.1 2,367,967 + +1.5 2,368,012 + -1.0 2,368,013 - -1.1 2,368,013 - -1.1 2,368,013 - +1.1 2,368,117 - -0.5 2,368,146 + -0.5 2,368,146 + +0.5 2,368,146 + -0.8 2,368,147 - +0.9 2,368,187 - +1.1 2,368,187 - -2.1 2,368,187 - +0.9 2,368,187 - -1.3 2,368,187 - +0.0 2,368,189 + -0.5 2,368,189 + +0.2 2,368,189 + +0.1 2,368,189 + +1.1 2,368,189 + +1.8 2,368,189 + -1.1 2,368,189 + -1.5 2,368,189 + +2.2 2,368,189 + -0.8 2,368,189 + -0.8 2,368,189 + -1.5 2,368,190 - -1.5 2,368,190 - -0.5 2,368,190 - +0.1 2,368,190 - -0.5 2,368,190 - -0.1 2,368,190 - +0.2 2,368,190 - -0.5 2,368,190 - +1.5 2,368,190 - +0.9 2,368,190 - +1.4 2,368,190 - +0.1 2,368,190 - +2.1 2,368,190 - -2.1 2,368,190 - -1.1 2,368,190 - +0.0
Or see this region's nucleotide sequence