Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_200 and Pf1N1B4_201 overlap by 4 nucleotides Pf1N1B4_201 and Pf1N1B4_202 overlap by 1 nucleotides Pf1N1B4_202 and Pf1N1B4_203 are separated by 0 nucleotides
Pf1N1B4_200 - FIG014801: Cation ABC transporter, periplasmic cation-binding protein, at 247,240 to 248,169
_200
Pf1N1B4_201 - ABC transporter in pyoverdin gene cluster, ATP-binding component, at 248,166 to 249,017
_201
Pf1N1B4_202 - ABC transporter in pyoverdin gene cluster, permease component, at 249,017 to 249,916
_202
Pf1N1B4_203 - ABC transporter in pyoverdin gene cluster, periplasmic component, at 249,917 to 250,888
_203
Position (kb) 249
250 Strain fitness (log2 ratio) -2
-1
0
1 at 248.020 kb on + strand, within Pf1N1B4_200 at 248.020 kb on + strand, within Pf1N1B4_200 at 248.021 kb on - strand, within Pf1N1B4_200 at 248.524 kb on - strand, within Pf1N1B4_201 at 248.558 kb on - strand, within Pf1N1B4_201 at 248.624 kb on + strand, within Pf1N1B4_201 at 248.625 kb on - strand, within Pf1N1B4_201 at 248.632 kb on - strand, within Pf1N1B4_201 at 249.023 kb on + strand at 249.024 kb on - strand at 249.251 kb on + strand at 249.251 kb on + strand, within Pf1N1B4_202 at 249.252 kb on - strand at 249.264 kb on - strand at 249.311 kb on + strand, within Pf1N1B4_202 at 249.311 kb on + strand at 249.311 kb on + strand at 249.311 kb on + strand, within Pf1N1B4_202 at 249.311 kb on + strand, within Pf1N1B4_202 at 249.312 kb on - strand, within Pf1N1B4_202 at 249.315 kb on + strand, within Pf1N1B4_202 at 249.315 kb on + strand, within Pf1N1B4_202 at 249.316 kb on - strand, within Pf1N1B4_202 at 249.316 kb on - strand, within Pf1N1B4_202 at 249.326 kb on - strand, within Pf1N1B4_202 at 249.533 kb on + strand, within Pf1N1B4_202 at 249.533 kb on + strand, within Pf1N1B4_202 at 249.534 kb on - strand, within Pf1N1B4_202 at 249.608 kb on + strand, within Pf1N1B4_202 at 250.103 kb on + strand, within Pf1N1B4_203 at 250.103 kb on + strand, within Pf1N1B4_203 at 250.104 kb on - strand, within Pf1N1B4_203 at 250.194 kb on - strand, within Pf1N1B4_203 at 250.226 kb on - strand, within Pf1N1B4_203 at 250.313 kb on - strand, within Pf1N1B4_203 at 250.500 kb on + strand, within Pf1N1B4_203 at 250.501 kb on - strand, within Pf1N1B4_203 at 250.737 kb on + strand, within Pf1N1B4_203 at 250.737 kb on + strand, within Pf1N1B4_203 at 250.737 kb on + strand, within Pf1N1B4_203 at 250.738 kb on - strand, within Pf1N1B4_203
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 248,020 + Pf1N1B4_200 0.84 +1.3 248,020 + Pf1N1B4_200 0.84 -0.6 248,021 - Pf1N1B4_200 0.84 -0.7 248,524 - Pf1N1B4_201 0.42 -0.3 248,558 - Pf1N1B4_201 0.46 -0.3 248,624 + Pf1N1B4_201 0.54 -1.3 248,625 - Pf1N1B4_201 0.54 -0.7 248,632 - Pf1N1B4_201 0.55 -0.3 249,023 + +0.4 249,024 - +0.7 249,251 + -1.9 249,251 + Pf1N1B4_202 0.26 -2.7 249,252 - +1.0 249,264 - -0.3 249,311 + Pf1N1B4_202 0.33 -2.6 249,311 + -1.9 249,311 + -0.3 249,311 + Pf1N1B4_202 0.33 -1.4 249,311 + Pf1N1B4_202 0.33 -1.3 249,312 - Pf1N1B4_202 0.33 +0.7 249,315 + Pf1N1B4_202 0.33 -2.3 249,315 + Pf1N1B4_202 0.33 +0.1 249,316 - Pf1N1B4_202 0.33 -0.0 249,316 - Pf1N1B4_202 0.33 -0.3 249,326 - Pf1N1B4_202 0.34 -2.3 249,533 + Pf1N1B4_202 0.57 -0.7 249,533 + Pf1N1B4_202 0.57 +0.7 249,534 - Pf1N1B4_202 0.57 -1.0 249,608 + Pf1N1B4_202 0.66 -1.3 250,103 + Pf1N1B4_203 0.19 -1.5 250,103 + Pf1N1B4_203 0.19 -1.0 250,104 - Pf1N1B4_203 0.19 -0.3 250,194 - Pf1N1B4_203 0.28 +0.2 250,226 - Pf1N1B4_203 0.32 -0.3 250,313 - Pf1N1B4_203 0.41 -1.3 250,500 + Pf1N1B4_203 0.60 +1.0 250,501 - Pf1N1B4_203 0.60 +0.4 250,737 + Pf1N1B4_203 0.84 -0.3 250,737 + Pf1N1B4_203 0.84 +0.4 250,737 + Pf1N1B4_203 0.84 -0.7 250,738 - Pf1N1B4_203 0.84 -1.3
Or see this region's nucleotide sequence