Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_2011

Experiment: L-Isoleucine (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_2010 and Pf1N1B4_2011 are separated by 11 nucleotidesPf1N1B4_2011 and Pf1N1B4_2012 are separated by 284 nucleotides Pf1N1B4_2010 - Pyruvate carboxyl transferase subunit A (EC 6.4.1.1), at 2,295,359 to 2,296,774 _2010 Pf1N1B4_2011 - Pyruvate carboxyl transferase subunit B (EC 6.4.1.1), at 2,296,786 to 2,298,594 _2011 Pf1N1B4_2012 - Amino acid ABC transporter, permease protein, at 2,298,879 to 2,299,661 _2012 Position (kb) 2296 2297 2298 2299Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3at 2296.115 kb on + strand, within Pf1N1B4_2010at 2296.116 kb on - strand, within Pf1N1B4_2010at 2296.116 kb on - strand, within Pf1N1B4_2010at 2296.116 kb on - strand, within Pf1N1B4_2010at 2296.205 kb on + strand, within Pf1N1B4_2010at 2296.205 kb on + strand, within Pf1N1B4_2010at 2296.205 kb on + strandat 2296.205 kb on + strand, within Pf1N1B4_2010at 2296.206 kb on - strand, within Pf1N1B4_2010at 2296.206 kb on - strand, within Pf1N1B4_2010at 2296.206 kb on - strandat 2296.262 kb on - strand, within Pf1N1B4_2010at 2296.262 kb on - strand, within Pf1N1B4_2010at 2296.427 kb on + strand, within Pf1N1B4_2010at 2296.427 kb on + strand, within Pf1N1B4_2010at 2296.469 kb on + strand, within Pf1N1B4_2010at 2296.469 kb on + strand, within Pf1N1B4_2010at 2296.470 kb on - strand, within Pf1N1B4_2010at 2296.470 kb on - strand, within Pf1N1B4_2010at 2296.470 kb on - strand, within Pf1N1B4_2010at 2296.470 kb on - strand, within Pf1N1B4_2010at 2296.470 kb on - strand, within Pf1N1B4_2010at 2296.496 kb on + strand, within Pf1N1B4_2010at 2296.496 kb on + strand, within Pf1N1B4_2010at 2296.496 kb on + strand, within Pf1N1B4_2010at 2296.497 kb on - strand, within Pf1N1B4_2010at 2296.613 kb on + strand, within Pf1N1B4_2010at 2296.614 kb on - strand, within Pf1N1B4_2010at 2296.614 kb on - strand, within Pf1N1B4_2010at 2296.614 kb on - strand, within Pf1N1B4_2010at 2296.700 kb on + strandat 2296.700 kb on + strandat 2296.700 kb on + strandat 2296.700 kb on + strandat 2296.700 kb on + strandat 2296.700 kb on + strandat 2296.701 kb on - strandat 2296.701 kb on - strandat 2296.701 kb on - strandat 2296.779 kb on + strandat 2296.779 kb on + strandat 2296.780 kb on - strandat 2296.780 kb on - strandat 2296.780 kb on - strandat 2296.780 kb on - strandat 2296.780 kb on - strandat 2296.953 kb on - strandat 2296.953 kb on - strandat 2297.010 kb on + strandat 2297.011 kb on - strand, within Pf1N1B4_2011at 2297.065 kb on + strand, within Pf1N1B4_2011at 2297.157 kb on + strand, within Pf1N1B4_2011at 2297.183 kb on + strand, within Pf1N1B4_2011at 2297.184 kb on - strand, within Pf1N1B4_2011at 2297.184 kb on - strand, within Pf1N1B4_2011at 2297.221 kb on + strand, within Pf1N1B4_2011at 2297.221 kb on + strand, within Pf1N1B4_2011at 2297.222 kb on - strand, within Pf1N1B4_2011at 2297.222 kb on - strand, within Pf1N1B4_2011at 2297.593 kb on + strand, within Pf1N1B4_2011at 2297.593 kb on + strand, within Pf1N1B4_2011at 2297.593 kb on + strand, within Pf1N1B4_2011at 2297.593 kb on + strand, within Pf1N1B4_2011at 2297.594 kb on - strand, within Pf1N1B4_2011at 2297.594 kb on - strand, within Pf1N1B4_2011at 2297.594 kb on - strand, within Pf1N1B4_2011at 2297.594 kb on - strand, within Pf1N1B4_2011at 2297.599 kb on + strand, within Pf1N1B4_2011at 2297.599 kb on + strand, within Pf1N1B4_2011at 2297.600 kb on - strand, within Pf1N1B4_2011at 2297.600 kb on - strandat 2297.600 kb on - strand, within Pf1N1B4_2011at 2297.617 kb on + strand, within Pf1N1B4_2011at 2297.617 kb on + strand, within Pf1N1B4_2011at 2297.617 kb on + strand, within Pf1N1B4_2011at 2297.617 kb on + strand, within Pf1N1B4_2011at 2297.617 kb on + strand, within Pf1N1B4_2011at 2297.617 kb on + strand, within Pf1N1B4_2011at 2297.618 kb on - strand, within Pf1N1B4_2011at 2297.618 kb on - strand, within Pf1N1B4_2011at 2297.861 kb on - strand, within Pf1N1B4_2011at 2297.874 kb on + strand, within Pf1N1B4_2011at 2297.874 kb on + strand, within Pf1N1B4_2011at 2297.891 kb on - strand, within Pf1N1B4_2011at 2297.891 kb on - strand, within Pf1N1B4_2011at 2297.891 kb on - strand, within Pf1N1B4_2011at 2297.891 kb on - strand, within Pf1N1B4_2011at 2298.067 kb on + strand, within Pf1N1B4_2011at 2298.067 kb on + strand, within Pf1N1B4_2011at 2298.067 kb on + strand, within Pf1N1B4_2011at 2298.068 kb on - strand, within Pf1N1B4_2011at 2298.068 kb on - strand, within Pf1N1B4_2011at 2298.230 kb on - strand, within Pf1N1B4_2011at 2298.283 kb on + strand, within Pf1N1B4_2011at 2298.283 kb on + strand, within Pf1N1B4_2011at 2298.283 kb on + strand, within Pf1N1B4_2011at 2298.283 kb on + strand, within Pf1N1B4_2011at 2298.283 kb on + strand, within Pf1N1B4_2011at 2298.284 kb on - strand, within Pf1N1B4_2011at 2298.284 kb on - strand, within Pf1N1B4_2011at 2298.284 kb on - strand, within Pf1N1B4_2011at 2298.284 kb on - strand, within Pf1N1B4_2011at 2298.284 kb on - strand, within Pf1N1B4_2011at 2298.284 kb on - strand, within Pf1N1B4_2011at 2298.284 kb on - strand, within Pf1N1B4_2011at 2298.284 kb on - strand, within Pf1N1B4_2011at 2298.572 kb on + strandat 2298.572 kb on + strandat 2298.572 kb on + strandat 2298.573 kb on - strandat 2298.573 kb on - strandat 2298.573 kb on - strandat 2298.597 kb on + strandat 2298.598 kb on - strandat 2298.611 kb on + strandat 2298.612 kb on - strandat 2298.612 kb on - strandat 2298.612 kb on - strandat 2298.619 kb on + strandat 2298.620 kb on - strandat 2298.717 kb on + strandat 2298.727 kb on + strandat 2298.727 kb on + strandat 2298.728 kb on - strandat 2298.742 kb on + strandat 2298.743 kb on - strandat 2298.743 kb on - strandat 2298.796 kb on + strandat 2298.796 kb on + strandat 2298.796 kb on + strandat 2298.796 kb on + strandat 2298.796 kb on + strandat 2298.797 kb on - strandat 2298.797 kb on - strandat 2298.797 kb on - strandat 2298.870 kb on + strandat 2298.870 kb on + strandat 2298.891 kb on + strandat 2298.891 kb on + strandat 2298.892 kb on - strandat 2298.892 kb on - strandat 2298.892 kb on - strandat 2298.892 kb on - strandat 2298.892 kb on - strandat 2298.892 kb on - strandat 2298.892 kb on - strandat 2298.904 kb on - strandat 2298.957 kb on + strandat 2298.957 kb on + strandat 2298.957 kb on + strandat 2298.957 kb on + strandat 2298.958 kb on - strand, within Pf1N1B4_2012at 2298.958 kb on - strand, within Pf1N1B4_2012at 2298.961 kb on + strand, within Pf1N1B4_2012at 2298.961 kb on + strand, within Pf1N1B4_2012at 2298.961 kb on + strand, within Pf1N1B4_2012at 2298.961 kb on + strand, within Pf1N1B4_2012at 2298.961 kb on + strand, within Pf1N1B4_2012at 2298.961 kb on + strand, within Pf1N1B4_2012at 2298.962 kb on - strand, within Pf1N1B4_2012at 2298.962 kb on - strand, within Pf1N1B4_2012at 2298.962 kb on - strand, within Pf1N1B4_2012at 2298.962 kb on - strand, within Pf1N1B4_2012at 2298.962 kb on - strand, within Pf1N1B4_2012at 2298.988 kb on + strand, within Pf1N1B4_2012at 2298.989 kb on - strand, within Pf1N1B4_2012at 2298.990 kb on + strand, within Pf1N1B4_2012at 2298.990 kb on + strand, within Pf1N1B4_2012at 2298.990 kb on + strand, within Pf1N1B4_2012at 2298.991 kb on - strand, within Pf1N1B4_2012at 2298.991 kb on - strand, within Pf1N1B4_2012at 2298.991 kb on - strand, within Pf1N1B4_2012at 2298.991 kb on - strand, within Pf1N1B4_2012at 2298.991 kb on - strand, within Pf1N1B4_2012at 2299.012 kb on - strand, within Pf1N1B4_2012at 2299.082 kb on - strand, within Pf1N1B4_2012at 2299.249 kb on + strand, within Pf1N1B4_2012at 2299.249 kb on + strand, within Pf1N1B4_2012at 2299.250 kb on - strand, within Pf1N1B4_2012at 2299.321 kb on + strand, within Pf1N1B4_2012at 2299.321 kb on + strand, within Pf1N1B4_2012at 2299.321 kb on + strand, within Pf1N1B4_2012at 2299.322 kb on - strand, within Pf1N1B4_2012at 2299.322 kb on - strand, within Pf1N1B4_2012at 2299.322 kb on - strand, within Pf1N1B4_2012at 2299.352 kb on + strand, within Pf1N1B4_2012at 2299.372 kb on + strand, within Pf1N1B4_2012at 2299.372 kb on + strand, within Pf1N1B4_2012at 2299.372 kb on + strand, within Pf1N1B4_2012at 2299.372 kb on + strand, within Pf1N1B4_2012at 2299.372 kb on + strand, within Pf1N1B4_2012at 2299.372 kb on + strand, within Pf1N1B4_2012at 2299.373 kb on - strand, within Pf1N1B4_2012at 2299.373 kb on - strand, within Pf1N1B4_2012at 2299.373 kb on - strand, within Pf1N1B4_2012at 2299.373 kb on - strand, within Pf1N1B4_2012at 2299.373 kb on - strand, within Pf1N1B4_2012at 2299.373 kb on - strand, within Pf1N1B4_2012at 2299.373 kb on - strand, within Pf1N1B4_2012at 2299.373 kb on - strand, within Pf1N1B4_2012at 2299.373 kb on - strand, within Pf1N1B4_2012at 2299.373 kb on - strand, within Pf1N1B4_2012at 2299.373 kb on - strand, within Pf1N1B4_2012at 2299.373 kb on - strand, within Pf1N1B4_2012

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Isoleucine (C)
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2,296,115 + Pf1N1B4_2010 0.53 -2.1
2,296,116 - Pf1N1B4_2010 0.53 -1.1
2,296,116 - Pf1N1B4_2010 0.53 -2.3
2,296,116 - Pf1N1B4_2010 0.53 -0.5
2,296,205 + Pf1N1B4_2010 0.60 -1.3
2,296,205 + Pf1N1B4_2010 0.60 -2.5
2,296,205 + +0.5
2,296,205 + Pf1N1B4_2010 0.60 -2.8
2,296,206 - Pf1N1B4_2010 0.60 -1.8
2,296,206 - Pf1N1B4_2010 0.60 -1.5
2,296,206 - +0.5
2,296,262 - Pf1N1B4_2010 0.64 -2.0
2,296,262 - Pf1N1B4_2010 0.64 -0.5
2,296,427 + Pf1N1B4_2010 0.75 -1.8
2,296,427 + Pf1N1B4_2010 0.75 +0.5
2,296,469 + Pf1N1B4_2010 0.78 -1.8
2,296,469 + Pf1N1B4_2010 0.78 -1.1
2,296,470 - Pf1N1B4_2010 0.78 -0.5
2,296,470 - Pf1N1B4_2010 0.78 -2.3
2,296,470 - Pf1N1B4_2010 0.78 -1.1
2,296,470 - Pf1N1B4_2010 0.78 -3.2
2,296,470 - Pf1N1B4_2010 0.78 -1.8
2,296,496 + Pf1N1B4_2010 0.80 -1.5
2,296,496 + Pf1N1B4_2010 0.80 -3.3
2,296,496 + Pf1N1B4_2010 0.80 -1.1
2,296,497 - Pf1N1B4_2010 0.80 -2.3
2,296,613 + Pf1N1B4_2010 0.89 -1.1
2,296,614 - Pf1N1B4_2010 0.89 +1.5
2,296,614 - Pf1N1B4_2010 0.89 -2.1
2,296,614 - Pf1N1B4_2010 0.89 -0.5
2,296,700 + -0.9
2,296,700 + -2.1
2,296,700 + -2.8
2,296,700 + -2.5
2,296,700 + -0.5
2,296,700 + -2.1
2,296,701 - -0.1
2,296,701 - -2.1
2,296,701 - +0.5
2,296,779 + -1.1
2,296,779 + +0.5
2,296,780 - -2.3
2,296,780 - -1.7
2,296,780 - -1.8
2,296,780 - -1.5
2,296,780 - -0.5
2,296,953 - -2.7
2,296,953 - -0.5
2,297,010 + -0.5
2,297,011 - Pf1N1B4_2011 0.12 -1.1
2,297,065 + Pf1N1B4_2011 0.15 -1.1
2,297,157 + Pf1N1B4_2011 0.21 -1.5
2,297,183 + Pf1N1B4_2011 0.22 -0.2
2,297,184 - Pf1N1B4_2011 0.22 -0.5
2,297,184 - Pf1N1B4_2011 0.22 -4.2
2,297,221 + Pf1N1B4_2011 0.24 -3.3
2,297,221 + Pf1N1B4_2011 0.24 -1.1
2,297,222 - Pf1N1B4_2011 0.24 -2.3
2,297,222 - Pf1N1B4_2011 0.24 -0.5
2,297,593 + Pf1N1B4_2011 0.45 -0.5
2,297,593 + Pf1N1B4_2011 0.45 +0.5
2,297,593 + Pf1N1B4_2011 0.45 -1.8
2,297,593 + Pf1N1B4_2011 0.45 +0.5
2,297,594 - Pf1N1B4_2011 0.45 +0.5
2,297,594 - Pf1N1B4_2011 0.45 -0.1
2,297,594 - Pf1N1B4_2011 0.45 -1.1
2,297,594 - Pf1N1B4_2011 0.45 -1.5
2,297,599 + Pf1N1B4_2011 0.45 -1.5
2,297,599 + Pf1N1B4_2011 0.45 +0.5
2,297,600 - Pf1N1B4_2011 0.45 -1.5
2,297,600 - +2.1
2,297,600 - Pf1N1B4_2011 0.45 -1.1
2,297,617 + Pf1N1B4_2011 0.46 +0.8
2,297,617 + Pf1N1B4_2011 0.46 -1.1
2,297,617 + Pf1N1B4_2011 0.46 -2.8
2,297,617 + Pf1N1B4_2011 0.46 -0.8
2,297,617 + Pf1N1B4_2011 0.46 -2.3
2,297,617 + Pf1N1B4_2011 0.46 -1.5
2,297,618 - Pf1N1B4_2011 0.46 -0.5
2,297,618 - Pf1N1B4_2011 0.46 -1.1
2,297,861 - Pf1N1B4_2011 0.59 -1.1
2,297,874 + Pf1N1B4_2011 0.60 -1.5
2,297,874 + Pf1N1B4_2011 0.60 -1.8
2,297,891 - Pf1N1B4_2011 0.61 -1.8
2,297,891 - Pf1N1B4_2011 0.61 -1.8
2,297,891 - Pf1N1B4_2011 0.61 -2.3
2,297,891 - Pf1N1B4_2011 0.61 -0.5
2,298,067 + Pf1N1B4_2011 0.71 -0.1
2,298,067 + Pf1N1B4_2011 0.71 -2.1
2,298,067 + Pf1N1B4_2011 0.71 +2.1
2,298,068 - Pf1N1B4_2011 0.71 -0.5
2,298,068 - Pf1N1B4_2011 0.71 -1.5
2,298,230 - Pf1N1B4_2011 0.80 -2.1
2,298,283 + Pf1N1B4_2011 0.83 -0.5
2,298,283 + Pf1N1B4_2011 0.83 -0.5
2,298,283 + Pf1N1B4_2011 0.83 -0.2
2,298,283 + Pf1N1B4_2011 0.83 -0.5
2,298,283 + Pf1N1B4_2011 0.83 -0.1
2,298,284 - Pf1N1B4_2011 0.83 -2.1
2,298,284 - Pf1N1B4_2011 0.83 -1.8
2,298,284 - Pf1N1B4_2011 0.83 -2.5
2,298,284 - Pf1N1B4_2011 0.83 -1.5
2,298,284 - Pf1N1B4_2011 0.83 -1.1
2,298,284 - Pf1N1B4_2011 0.83 +2.5
2,298,284 - Pf1N1B4_2011 0.83 +0.5
2,298,284 - Pf1N1B4_2011 0.83 -0.5
2,298,572 + +0.5
2,298,572 + -3.7
2,298,572 + -0.5
2,298,573 - -0.5
2,298,573 - -1.5
2,298,573 - -1.1
2,298,597 + -0.8
2,298,598 - -0.2
2,298,611 + -0.5
2,298,612 - +1.5
2,298,612 - -0.5
2,298,612 - -1.3
2,298,619 + +0.5
2,298,620 - -1.2
2,298,717 + -1.8
2,298,727 + +0.3
2,298,727 + -1.5
2,298,728 - -0.9
2,298,742 + -1.1
2,298,743 - +1.5
2,298,743 - +0.9
2,298,796 + -0.5
2,298,796 + +1.5
2,298,796 + +0.5
2,298,796 + -0.7
2,298,796 + -0.1
2,298,797 - -1.1
2,298,797 - -0.7
2,298,797 - +1.3
2,298,870 + -1.1
2,298,870 + -1.1
2,298,891 + -0.8
2,298,891 + +0.5
2,298,892 - -0.8
2,298,892 - +0.9
2,298,892 - +0.2
2,298,892 - -0.8
2,298,892 - +1.5
2,298,892 - -0.2
2,298,892 - +0.0
2,298,904 - -0.5
2,298,957 + +0.3
2,298,957 + +0.1
2,298,957 + -1.7
2,298,957 + -1.1
2,298,958 - Pf1N1B4_2012 0.10 +1.1
2,298,958 - Pf1N1B4_2012 0.10 -0.3
2,298,961 + Pf1N1B4_2012 0.10 -1.1
2,298,961 + Pf1N1B4_2012 0.10 -0.9
2,298,961 + Pf1N1B4_2012 0.10 +0.9
2,298,961 + Pf1N1B4_2012 0.10 -2.3
2,298,961 + Pf1N1B4_2012 0.10 -0.8
2,298,961 + Pf1N1B4_2012 0.10 -1.8
2,298,962 - Pf1N1B4_2012 0.11 -1.2
2,298,962 - Pf1N1B4_2012 0.11 -0.2
2,298,962 - Pf1N1B4_2012 0.11 -1.1
2,298,962 - Pf1N1B4_2012 0.11 +1.0
2,298,962 - Pf1N1B4_2012 0.11 +0.5
2,298,988 + Pf1N1B4_2012 0.14 -1.1
2,298,989 - Pf1N1B4_2012 0.14 +0.8
2,298,990 + Pf1N1B4_2012 0.14 +0.5
2,298,990 + Pf1N1B4_2012 0.14 +0.3
2,298,990 + Pf1N1B4_2012 0.14 -0.1
2,298,991 - Pf1N1B4_2012 0.14 -1.3
2,298,991 - Pf1N1B4_2012 0.14 +1.5
2,298,991 - Pf1N1B4_2012 0.14 -1.1
2,298,991 - Pf1N1B4_2012 0.14 -1.8
2,298,991 - Pf1N1B4_2012 0.14 -0.4
2,299,012 - Pf1N1B4_2012 0.17 +0.2
2,299,082 - Pf1N1B4_2012 0.26 +0.2
2,299,249 + Pf1N1B4_2012 0.47 -1.1
2,299,249 + Pf1N1B4_2012 0.47 -0.4
2,299,250 - Pf1N1B4_2012 0.47 -0.8
2,299,321 + Pf1N1B4_2012 0.56 -0.3
2,299,321 + Pf1N1B4_2012 0.56 -0.5
2,299,321 + Pf1N1B4_2012 0.56 -0.4
2,299,322 - Pf1N1B4_2012 0.57 -0.5
2,299,322 - Pf1N1B4_2012 0.57 +1.1
2,299,322 - Pf1N1B4_2012 0.57 +0.8
2,299,352 + Pf1N1B4_2012 0.60 -1.8
2,299,372 + Pf1N1B4_2012 0.63 -1.8
2,299,372 + Pf1N1B4_2012 0.63 +0.1
2,299,372 + Pf1N1B4_2012 0.63 -0.4
2,299,372 + Pf1N1B4_2012 0.63 -0.5
2,299,372 + Pf1N1B4_2012 0.63 -0.1
2,299,372 + Pf1N1B4_2012 0.63 +0.3
2,299,373 - Pf1N1B4_2012 0.63 +0.0
2,299,373 - Pf1N1B4_2012 0.63 +0.2
2,299,373 - Pf1N1B4_2012 0.63 -0.1
2,299,373 - Pf1N1B4_2012 0.63 -1.3
2,299,373 - Pf1N1B4_2012 0.63 +0.5
2,299,373 - Pf1N1B4_2012 0.63 +1.5
2,299,373 - Pf1N1B4_2012 0.63 +0.5
2,299,373 - Pf1N1B4_2012 0.63 -1.1
2,299,373 - Pf1N1B4_2012 0.63 +0.5
2,299,373 - Pf1N1B4_2012 0.63 -0.8
2,299,373 - Pf1N1B4_2012 0.63 -0.1
2,299,373 - Pf1N1B4_2012 0.63 +2.8

Or see this region's nucleotide sequence