Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2010 and Pf1N1B4_2011 are separated by 11 nucleotides Pf1N1B4_2011 and Pf1N1B4_2012 are separated by 284 nucleotides
Pf1N1B4_2010 - Pyruvate carboxyl transferase subunit A (EC 6.4.1.1), at 2,295,359 to 2,296,774
_2010
Pf1N1B4_2011 - Pyruvate carboxyl transferase subunit B (EC 6.4.1.1), at 2,296,786 to 2,298,594
_2011
Pf1N1B4_2012 - Amino acid ABC transporter, permease protein, at 2,298,879 to 2,299,661
_2012
Position (kb)
2296
2297
2298
2299 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 2296.115 kb on + strand, within Pf1N1B4_2010 at 2296.116 kb on - strand, within Pf1N1B4_2010 at 2296.116 kb on - strand, within Pf1N1B4_2010 at 2296.116 kb on - strand, within Pf1N1B4_2010 at 2296.205 kb on + strand, within Pf1N1B4_2010 at 2296.205 kb on + strand, within Pf1N1B4_2010 at 2296.205 kb on + strand at 2296.205 kb on + strand, within Pf1N1B4_2010 at 2296.206 kb on - strand, within Pf1N1B4_2010 at 2296.206 kb on - strand, within Pf1N1B4_2010 at 2296.206 kb on - strand at 2296.262 kb on - strand, within Pf1N1B4_2010 at 2296.262 kb on - strand, within Pf1N1B4_2010 at 2296.427 kb on + strand, within Pf1N1B4_2010 at 2296.427 kb on + strand, within Pf1N1B4_2010 at 2296.469 kb on + strand, within Pf1N1B4_2010 at 2296.469 kb on + strand, within Pf1N1B4_2010 at 2296.470 kb on - strand, within Pf1N1B4_2010 at 2296.470 kb on - strand, within Pf1N1B4_2010 at 2296.470 kb on - strand, within Pf1N1B4_2010 at 2296.470 kb on - strand, within Pf1N1B4_2010 at 2296.470 kb on - strand, within Pf1N1B4_2010 at 2296.496 kb on + strand, within Pf1N1B4_2010 at 2296.496 kb on + strand, within Pf1N1B4_2010 at 2296.496 kb on + strand, within Pf1N1B4_2010 at 2296.497 kb on - strand, within Pf1N1B4_2010 at 2296.613 kb on + strand, within Pf1N1B4_2010 at 2296.614 kb on - strand, within Pf1N1B4_2010 at 2296.614 kb on - strand, within Pf1N1B4_2010 at 2296.614 kb on - strand, within Pf1N1B4_2010 at 2296.700 kb on + strand at 2296.700 kb on + strand at 2296.700 kb on + strand at 2296.700 kb on + strand at 2296.700 kb on + strand at 2296.700 kb on + strand at 2296.701 kb on - strand at 2296.701 kb on - strand at 2296.701 kb on - strand at 2296.779 kb on + strand at 2296.779 kb on + strand at 2296.780 kb on - strand at 2296.780 kb on - strand at 2296.780 kb on - strand at 2296.780 kb on - strand at 2296.780 kb on - strand at 2296.953 kb on - strand at 2296.953 kb on - strand at 2297.010 kb on + strand at 2297.011 kb on - strand, within Pf1N1B4_2011 at 2297.065 kb on + strand, within Pf1N1B4_2011 at 2297.157 kb on + strand, within Pf1N1B4_2011 at 2297.183 kb on + strand, within Pf1N1B4_2011 at 2297.184 kb on - strand, within Pf1N1B4_2011 at 2297.184 kb on - strand, within Pf1N1B4_2011 at 2297.221 kb on + strand, within Pf1N1B4_2011 at 2297.221 kb on + strand, within Pf1N1B4_2011 at 2297.222 kb on - strand, within Pf1N1B4_2011 at 2297.222 kb on - strand, within Pf1N1B4_2011 at 2297.593 kb on + strand, within Pf1N1B4_2011 at 2297.593 kb on + strand, within Pf1N1B4_2011 at 2297.593 kb on + strand, within Pf1N1B4_2011 at 2297.593 kb on + strand, within Pf1N1B4_2011 at 2297.594 kb on - strand, within Pf1N1B4_2011 at 2297.594 kb on - strand, within Pf1N1B4_2011 at 2297.594 kb on - strand, within Pf1N1B4_2011 at 2297.594 kb on - strand, within Pf1N1B4_2011 at 2297.599 kb on + strand, within Pf1N1B4_2011 at 2297.599 kb on + strand, within Pf1N1B4_2011 at 2297.600 kb on - strand, within Pf1N1B4_2011 at 2297.600 kb on - strand at 2297.600 kb on - strand, within Pf1N1B4_2011 at 2297.617 kb on + strand, within Pf1N1B4_2011 at 2297.617 kb on + strand, within Pf1N1B4_2011 at 2297.617 kb on + strand, within Pf1N1B4_2011 at 2297.617 kb on + strand, within Pf1N1B4_2011 at 2297.617 kb on + strand, within Pf1N1B4_2011 at 2297.617 kb on + strand, within Pf1N1B4_2011 at 2297.618 kb on - strand, within Pf1N1B4_2011 at 2297.618 kb on - strand, within Pf1N1B4_2011 at 2297.861 kb on - strand, within Pf1N1B4_2011 at 2297.874 kb on + strand, within Pf1N1B4_2011 at 2297.874 kb on + strand, within Pf1N1B4_2011 at 2297.891 kb on - strand, within Pf1N1B4_2011 at 2297.891 kb on - strand, within Pf1N1B4_2011 at 2297.891 kb on - strand, within Pf1N1B4_2011 at 2297.891 kb on - strand, within Pf1N1B4_2011 at 2298.067 kb on + strand, within Pf1N1B4_2011 at 2298.067 kb on + strand, within Pf1N1B4_2011 at 2298.067 kb on + strand, within Pf1N1B4_2011 at 2298.068 kb on - strand, within Pf1N1B4_2011 at 2298.068 kb on - strand, within Pf1N1B4_2011 at 2298.230 kb on - strand, within Pf1N1B4_2011 at 2298.283 kb on + strand, within Pf1N1B4_2011 at 2298.283 kb on + strand, within Pf1N1B4_2011 at 2298.283 kb on + strand, within Pf1N1B4_2011 at 2298.283 kb on + strand, within Pf1N1B4_2011 at 2298.283 kb on + strand, within Pf1N1B4_2011 at 2298.284 kb on - strand, within Pf1N1B4_2011 at 2298.284 kb on - strand, within Pf1N1B4_2011 at 2298.284 kb on - strand, within Pf1N1B4_2011 at 2298.284 kb on - strand, within Pf1N1B4_2011 at 2298.284 kb on - strand, within Pf1N1B4_2011 at 2298.284 kb on - strand, within Pf1N1B4_2011 at 2298.284 kb on - strand, within Pf1N1B4_2011 at 2298.284 kb on - strand, within Pf1N1B4_2011 at 2298.572 kb on + strand at 2298.572 kb on + strand at 2298.572 kb on + strand at 2298.573 kb on - strand at 2298.573 kb on - strand at 2298.573 kb on - strand at 2298.597 kb on + strand at 2298.598 kb on - strand at 2298.611 kb on + strand at 2298.612 kb on - strand at 2298.612 kb on - strand at 2298.612 kb on - strand at 2298.619 kb on + strand at 2298.620 kb on - strand at 2298.717 kb on + strand at 2298.727 kb on + strand at 2298.727 kb on + strand at 2298.728 kb on - strand at 2298.742 kb on + strand at 2298.743 kb on - strand at 2298.743 kb on - strand at 2298.796 kb on + strand at 2298.796 kb on + strand at 2298.796 kb on + strand at 2298.796 kb on + strand at 2298.796 kb on + strand at 2298.797 kb on - strand at 2298.797 kb on - strand at 2298.797 kb on - strand at 2298.870 kb on + strand at 2298.870 kb on + strand at 2298.891 kb on + strand at 2298.891 kb on + strand at 2298.892 kb on - strand at 2298.892 kb on - strand at 2298.892 kb on - strand at 2298.892 kb on - strand at 2298.892 kb on - strand at 2298.892 kb on - strand at 2298.892 kb on - strand at 2298.904 kb on - strand at 2298.957 kb on + strand at 2298.957 kb on + strand at 2298.957 kb on + strand at 2298.957 kb on + strand at 2298.958 kb on - strand, within Pf1N1B4_2012 at 2298.958 kb on - strand, within Pf1N1B4_2012 at 2298.961 kb on + strand, within Pf1N1B4_2012 at 2298.961 kb on + strand, within Pf1N1B4_2012 at 2298.961 kb on + strand, within Pf1N1B4_2012 at 2298.961 kb on + strand, within Pf1N1B4_2012 at 2298.961 kb on + strand, within Pf1N1B4_2012 at 2298.961 kb on + strand, within Pf1N1B4_2012 at 2298.962 kb on - strand, within Pf1N1B4_2012 at 2298.962 kb on - strand, within Pf1N1B4_2012 at 2298.962 kb on - strand, within Pf1N1B4_2012 at 2298.962 kb on - strand, within Pf1N1B4_2012 at 2298.962 kb on - strand, within Pf1N1B4_2012 at 2298.988 kb on + strand, within Pf1N1B4_2012 at 2298.989 kb on - strand, within Pf1N1B4_2012 at 2298.990 kb on + strand, within Pf1N1B4_2012 at 2298.990 kb on + strand, within Pf1N1B4_2012 at 2298.990 kb on + strand, within Pf1N1B4_2012 at 2298.991 kb on - strand, within Pf1N1B4_2012 at 2298.991 kb on - strand, within Pf1N1B4_2012 at 2298.991 kb on - strand, within Pf1N1B4_2012 at 2298.991 kb on - strand, within Pf1N1B4_2012 at 2298.991 kb on - strand, within Pf1N1B4_2012 at 2299.012 kb on - strand, within Pf1N1B4_2012 at 2299.082 kb on - strand, within Pf1N1B4_2012 at 2299.249 kb on + strand, within Pf1N1B4_2012 at 2299.249 kb on + strand, within Pf1N1B4_2012 at 2299.250 kb on - strand, within Pf1N1B4_2012 at 2299.321 kb on + strand, within Pf1N1B4_2012 at 2299.321 kb on + strand, within Pf1N1B4_2012 at 2299.321 kb on + strand, within Pf1N1B4_2012 at 2299.322 kb on - strand, within Pf1N1B4_2012 at 2299.322 kb on - strand, within Pf1N1B4_2012 at 2299.322 kb on - strand, within Pf1N1B4_2012 at 2299.352 kb on + strand, within Pf1N1B4_2012 at 2299.372 kb on + strand, within Pf1N1B4_2012 at 2299.372 kb on + strand, within Pf1N1B4_2012 at 2299.372 kb on + strand, within Pf1N1B4_2012 at 2299.372 kb on + strand, within Pf1N1B4_2012 at 2299.372 kb on + strand, within Pf1N1B4_2012 at 2299.372 kb on + strand, within Pf1N1B4_2012 at 2299.373 kb on - strand, within Pf1N1B4_2012 at 2299.373 kb on - strand, within Pf1N1B4_2012 at 2299.373 kb on - strand, within Pf1N1B4_2012 at 2299.373 kb on - strand, within Pf1N1B4_2012 at 2299.373 kb on - strand, within Pf1N1B4_2012 at 2299.373 kb on - strand, within Pf1N1B4_2012 at 2299.373 kb on - strand, within Pf1N1B4_2012 at 2299.373 kb on - strand, within Pf1N1B4_2012 at 2299.373 kb on - strand, within Pf1N1B4_2012 at 2299.373 kb on - strand, within Pf1N1B4_2012 at 2299.373 kb on - strand, within Pf1N1B4_2012 at 2299.373 kb on - strand, within Pf1N1B4_2012
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 2,296,115 + Pf1N1B4_2010 0.53 -2.1 2,296,116 - Pf1N1B4_2010 0.53 -1.1 2,296,116 - Pf1N1B4_2010 0.53 -2.3 2,296,116 - Pf1N1B4_2010 0.53 -0.5 2,296,205 + Pf1N1B4_2010 0.60 -1.3 2,296,205 + Pf1N1B4_2010 0.60 -2.5 2,296,205 + +0.5 2,296,205 + Pf1N1B4_2010 0.60 -2.8 2,296,206 - Pf1N1B4_2010 0.60 -1.8 2,296,206 - Pf1N1B4_2010 0.60 -1.5 2,296,206 - +0.5 2,296,262 - Pf1N1B4_2010 0.64 -2.0 2,296,262 - Pf1N1B4_2010 0.64 -0.5 2,296,427 + Pf1N1B4_2010 0.75 -1.8 2,296,427 + Pf1N1B4_2010 0.75 +0.5 2,296,469 + Pf1N1B4_2010 0.78 -1.8 2,296,469 + Pf1N1B4_2010 0.78 -1.1 2,296,470 - Pf1N1B4_2010 0.78 -0.5 2,296,470 - Pf1N1B4_2010 0.78 -2.3 2,296,470 - Pf1N1B4_2010 0.78 -1.1 2,296,470 - Pf1N1B4_2010 0.78 -3.2 2,296,470 - Pf1N1B4_2010 0.78 -1.8 2,296,496 + Pf1N1B4_2010 0.80 -1.5 2,296,496 + Pf1N1B4_2010 0.80 -3.3 2,296,496 + Pf1N1B4_2010 0.80 -1.1 2,296,497 - Pf1N1B4_2010 0.80 -2.3 2,296,613 + Pf1N1B4_2010 0.89 -1.1 2,296,614 - Pf1N1B4_2010 0.89 +1.5 2,296,614 - Pf1N1B4_2010 0.89 -2.1 2,296,614 - Pf1N1B4_2010 0.89 -0.5 2,296,700 + -0.9 2,296,700 + -2.1 2,296,700 + -2.8 2,296,700 + -2.5 2,296,700 + -0.5 2,296,700 + -2.1 2,296,701 - -0.1 2,296,701 - -2.1 2,296,701 - +0.5 2,296,779 + -1.1 2,296,779 + +0.5 2,296,780 - -2.3 2,296,780 - -1.7 2,296,780 - -1.8 2,296,780 - -1.5 2,296,780 - -0.5 2,296,953 - -2.7 2,296,953 - -0.5 2,297,010 + -0.5 2,297,011 - Pf1N1B4_2011 0.12 -1.1 2,297,065 + Pf1N1B4_2011 0.15 -1.1 2,297,157 + Pf1N1B4_2011 0.21 -1.5 2,297,183 + Pf1N1B4_2011 0.22 -0.2 2,297,184 - Pf1N1B4_2011 0.22 -0.5 2,297,184 - Pf1N1B4_2011 0.22 -4.2 2,297,221 + Pf1N1B4_2011 0.24 -3.3 2,297,221 + Pf1N1B4_2011 0.24 -1.1 2,297,222 - Pf1N1B4_2011 0.24 -2.3 2,297,222 - Pf1N1B4_2011 0.24 -0.5 2,297,593 + Pf1N1B4_2011 0.45 -0.5 2,297,593 + Pf1N1B4_2011 0.45 +0.5 2,297,593 + Pf1N1B4_2011 0.45 -1.8 2,297,593 + Pf1N1B4_2011 0.45 +0.5 2,297,594 - Pf1N1B4_2011 0.45 +0.5 2,297,594 - Pf1N1B4_2011 0.45 -0.1 2,297,594 - Pf1N1B4_2011 0.45 -1.1 2,297,594 - Pf1N1B4_2011 0.45 -1.5 2,297,599 + Pf1N1B4_2011 0.45 -1.5 2,297,599 + Pf1N1B4_2011 0.45 +0.5 2,297,600 - Pf1N1B4_2011 0.45 -1.5 2,297,600 - +2.1 2,297,600 - Pf1N1B4_2011 0.45 -1.1 2,297,617 + Pf1N1B4_2011 0.46 +0.8 2,297,617 + Pf1N1B4_2011 0.46 -1.1 2,297,617 + Pf1N1B4_2011 0.46 -2.8 2,297,617 + Pf1N1B4_2011 0.46 -0.8 2,297,617 + Pf1N1B4_2011 0.46 -2.3 2,297,617 + Pf1N1B4_2011 0.46 -1.5 2,297,618 - Pf1N1B4_2011 0.46 -0.5 2,297,618 - Pf1N1B4_2011 0.46 -1.1 2,297,861 - Pf1N1B4_2011 0.59 -1.1 2,297,874 + Pf1N1B4_2011 0.60 -1.5 2,297,874 + Pf1N1B4_2011 0.60 -1.8 2,297,891 - Pf1N1B4_2011 0.61 -1.8 2,297,891 - Pf1N1B4_2011 0.61 -1.8 2,297,891 - Pf1N1B4_2011 0.61 -2.3 2,297,891 - Pf1N1B4_2011 0.61 -0.5 2,298,067 + Pf1N1B4_2011 0.71 -0.1 2,298,067 + Pf1N1B4_2011 0.71 -2.1 2,298,067 + Pf1N1B4_2011 0.71 +2.1 2,298,068 - Pf1N1B4_2011 0.71 -0.5 2,298,068 - Pf1N1B4_2011 0.71 -1.5 2,298,230 - Pf1N1B4_2011 0.80 -2.1 2,298,283 + Pf1N1B4_2011 0.83 -0.5 2,298,283 + Pf1N1B4_2011 0.83 -0.5 2,298,283 + Pf1N1B4_2011 0.83 -0.2 2,298,283 + Pf1N1B4_2011 0.83 -0.5 2,298,283 + Pf1N1B4_2011 0.83 -0.1 2,298,284 - Pf1N1B4_2011 0.83 -2.1 2,298,284 - Pf1N1B4_2011 0.83 -1.8 2,298,284 - Pf1N1B4_2011 0.83 -2.5 2,298,284 - Pf1N1B4_2011 0.83 -1.5 2,298,284 - Pf1N1B4_2011 0.83 -1.1 2,298,284 - Pf1N1B4_2011 0.83 +2.5 2,298,284 - Pf1N1B4_2011 0.83 +0.5 2,298,284 - Pf1N1B4_2011 0.83 -0.5 2,298,572 + +0.5 2,298,572 + -3.7 2,298,572 + -0.5 2,298,573 - -0.5 2,298,573 - -1.5 2,298,573 - -1.1 2,298,597 + -0.8 2,298,598 - -0.2 2,298,611 + -0.5 2,298,612 - +1.5 2,298,612 - -0.5 2,298,612 - -1.3 2,298,619 + +0.5 2,298,620 - -1.2 2,298,717 + -1.8 2,298,727 + +0.3 2,298,727 + -1.5 2,298,728 - -0.9 2,298,742 + -1.1 2,298,743 - +1.5 2,298,743 - +0.9 2,298,796 + -0.5 2,298,796 + +1.5 2,298,796 + +0.5 2,298,796 + -0.7 2,298,796 + -0.1 2,298,797 - -1.1 2,298,797 - -0.7 2,298,797 - +1.3 2,298,870 + -1.1 2,298,870 + -1.1 2,298,891 + -0.8 2,298,891 + +0.5 2,298,892 - -0.8 2,298,892 - +0.9 2,298,892 - +0.2 2,298,892 - -0.8 2,298,892 - +1.5 2,298,892 - -0.2 2,298,892 - +0.0 2,298,904 - -0.5 2,298,957 + +0.3 2,298,957 + +0.1 2,298,957 + -1.7 2,298,957 + -1.1 2,298,958 - Pf1N1B4_2012 0.10 +1.1 2,298,958 - Pf1N1B4_2012 0.10 -0.3 2,298,961 + Pf1N1B4_2012 0.10 -1.1 2,298,961 + Pf1N1B4_2012 0.10 -0.9 2,298,961 + Pf1N1B4_2012 0.10 +0.9 2,298,961 + Pf1N1B4_2012 0.10 -2.3 2,298,961 + Pf1N1B4_2012 0.10 -0.8 2,298,961 + Pf1N1B4_2012 0.10 -1.8 2,298,962 - Pf1N1B4_2012 0.11 -1.2 2,298,962 - Pf1N1B4_2012 0.11 -0.2 2,298,962 - Pf1N1B4_2012 0.11 -1.1 2,298,962 - Pf1N1B4_2012 0.11 +1.0 2,298,962 - Pf1N1B4_2012 0.11 +0.5 2,298,988 + Pf1N1B4_2012 0.14 -1.1 2,298,989 - Pf1N1B4_2012 0.14 +0.8 2,298,990 + Pf1N1B4_2012 0.14 +0.5 2,298,990 + Pf1N1B4_2012 0.14 +0.3 2,298,990 + Pf1N1B4_2012 0.14 -0.1 2,298,991 - Pf1N1B4_2012 0.14 -1.3 2,298,991 - Pf1N1B4_2012 0.14 +1.5 2,298,991 - Pf1N1B4_2012 0.14 -1.1 2,298,991 - Pf1N1B4_2012 0.14 -1.8 2,298,991 - Pf1N1B4_2012 0.14 -0.4 2,299,012 - Pf1N1B4_2012 0.17 +0.2 2,299,082 - Pf1N1B4_2012 0.26 +0.2 2,299,249 + Pf1N1B4_2012 0.47 -1.1 2,299,249 + Pf1N1B4_2012 0.47 -0.4 2,299,250 - Pf1N1B4_2012 0.47 -0.8 2,299,321 + Pf1N1B4_2012 0.56 -0.3 2,299,321 + Pf1N1B4_2012 0.56 -0.5 2,299,321 + Pf1N1B4_2012 0.56 -0.4 2,299,322 - Pf1N1B4_2012 0.57 -0.5 2,299,322 - Pf1N1B4_2012 0.57 +1.1 2,299,322 - Pf1N1B4_2012 0.57 +0.8 2,299,352 + Pf1N1B4_2012 0.60 -1.8 2,299,372 + Pf1N1B4_2012 0.63 -1.8 2,299,372 + Pf1N1B4_2012 0.63 +0.1 2,299,372 + Pf1N1B4_2012 0.63 -0.4 2,299,372 + Pf1N1B4_2012 0.63 -0.5 2,299,372 + Pf1N1B4_2012 0.63 -0.1 2,299,372 + Pf1N1B4_2012 0.63 +0.3 2,299,373 - Pf1N1B4_2012 0.63 +0.0 2,299,373 - Pf1N1B4_2012 0.63 +0.2 2,299,373 - Pf1N1B4_2012 0.63 -0.1 2,299,373 - Pf1N1B4_2012 0.63 -1.3 2,299,373 - Pf1N1B4_2012 0.63 +0.5 2,299,373 - Pf1N1B4_2012 0.63 +1.5 2,299,373 - Pf1N1B4_2012 0.63 +0.5 2,299,373 - Pf1N1B4_2012 0.63 -1.1 2,299,373 - Pf1N1B4_2012 0.63 +0.5 2,299,373 - Pf1N1B4_2012 0.63 -0.8 2,299,373 - Pf1N1B4_2012 0.63 -0.1 2,299,373 - Pf1N1B4_2012 0.63 +2.8
Or see this region's nucleotide sequence