Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2008 and Pf1N1B4_2009 are separated by 406 nucleotides Pf1N1B4_2009 and Pf1N1B4_2010 are separated by 211 nucleotides
Pf1N1B4_2008 - Phosphogluconate repressor HexR, RpiR family, at 2,292,933 to 2,293,799
_2008
Pf1N1B4_2009 - LysR family transcriptional regulator PA5437, at 2,294,206 to 2,295,147
_2009
Pf1N1B4_2010 - Pyruvate carboxyl transferase subunit A (EC 6.4.1.1), at 2,295,359 to 2,296,774
_2010
Position (kb)
2294
2295
2296 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 2293.212 kb on + strand, within Pf1N1B4_2008 at 2293.212 kb on + strand, within Pf1N1B4_2008 at 2293.213 kb on - strand, within Pf1N1B4_2008 at 2293.244 kb on + strand, within Pf1N1B4_2008 at 2293.244 kb on + strand, within Pf1N1B4_2008 at 2293.244 kb on + strand, within Pf1N1B4_2008 at 2293.244 kb on + strand, within Pf1N1B4_2008 at 2293.244 kb on + strand, within Pf1N1B4_2008 at 2293.244 kb on + strand, within Pf1N1B4_2008 at 2293.244 kb on + strand, within Pf1N1B4_2008 at 2293.244 kb on + strand, within Pf1N1B4_2008 at 2293.244 kb on + strand, within Pf1N1B4_2008 at 2293.244 kb on + strand, within Pf1N1B4_2008 at 2293.244 kb on + strand, within Pf1N1B4_2008 at 2293.245 kb on - strand, within Pf1N1B4_2008 at 2293.245 kb on - strand, within Pf1N1B4_2008 at 2293.245 kb on - strand, within Pf1N1B4_2008 at 2293.245 kb on - strand, within Pf1N1B4_2008 at 2293.245 kb on - strand, within Pf1N1B4_2008 at 2293.245 kb on - strand, within Pf1N1B4_2008 at 2293.245 kb on - strand, within Pf1N1B4_2008 at 2293.332 kb on + strand, within Pf1N1B4_2008 at 2293.332 kb on + strand, within Pf1N1B4_2008 at 2293.332 kb on + strand, within Pf1N1B4_2008 at 2293.332 kb on + strand, within Pf1N1B4_2008 at 2293.332 kb on + strand, within Pf1N1B4_2008 at 2293.332 kb on + strand, within Pf1N1B4_2008 at 2293.332 kb on + strand, within Pf1N1B4_2008 at 2293.333 kb on - strand, within Pf1N1B4_2008 at 2293.333 kb on - strand, within Pf1N1B4_2008 at 2293.333 kb on - strand, within Pf1N1B4_2008 at 2293.333 kb on - strand, within Pf1N1B4_2008 at 2293.333 kb on - strand, within Pf1N1B4_2008 at 2293.333 kb on - strand, within Pf1N1B4_2008 at 2293.390 kb on + strand, within Pf1N1B4_2008 at 2293.390 kb on + strand, within Pf1N1B4_2008 at 2293.390 kb on + strand, within Pf1N1B4_2008 at 2293.410 kb on + strand, within Pf1N1B4_2008 at 2293.411 kb on - strand, within Pf1N1B4_2008 at 2293.475 kb on + strand, within Pf1N1B4_2008 at 2293.475 kb on + strand, within Pf1N1B4_2008 at 2293.475 kb on + strand, within Pf1N1B4_2008 at 2293.475 kb on + strand, within Pf1N1B4_2008 at 2293.475 kb on + strand, within Pf1N1B4_2008 at 2293.475 kb on + strand, within Pf1N1B4_2008 at 2293.475 kb on + strand, within Pf1N1B4_2008 at 2293.476 kb on - strand, within Pf1N1B4_2008 at 2293.476 kb on - strand, within Pf1N1B4_2008 at 2293.476 kb on - strand, within Pf1N1B4_2008 at 2293.632 kb on + strand, within Pf1N1B4_2008 at 2293.632 kb on + strand, within Pf1N1B4_2008 at 2293.632 kb on + strand, within Pf1N1B4_2008 at 2293.632 kb on + strand, within Pf1N1B4_2008 at 2293.632 kb on + strand, within Pf1N1B4_2008 at 2293.632 kb on + strand, within Pf1N1B4_2008 at 2293.632 kb on + strand, within Pf1N1B4_2008 at 2293.632 kb on + strand, within Pf1N1B4_2008 at 2293.632 kb on + strand, within Pf1N1B4_2008 at 2293.633 kb on - strand, within Pf1N1B4_2008 at 2293.633 kb on - strand, within Pf1N1B4_2008 at 2293.633 kb on - strand at 2293.633 kb on - strand, within Pf1N1B4_2008 at 2293.633 kb on - strand, within Pf1N1B4_2008 at 2293.634 kb on + strand, within Pf1N1B4_2008 at 2293.635 kb on - strand, within Pf1N1B4_2008 at 2293.666 kb on + strand, within Pf1N1B4_2008 at 2293.946 kb on - strand at 2293.946 kb on - strand at 2293.946 kb on - strand at 2294.014 kb on + strand at 2294.014 kb on + strand at 2294.014 kb on + strand at 2294.014 kb on + strand at 2294.014 kb on + strand at 2294.014 kb on + strand at 2294.014 kb on + strand at 2294.014 kb on + strand at 2294.015 kb on - strand at 2294.015 kb on - strand at 2294.015 kb on - strand at 2294.015 kb on - strand at 2294.015 kb on - strand at 2294.015 kb on - strand at 2294.015 kb on - strand at 2294.015 kb on - strand at 2294.015 kb on - strand at 2294.021 kb on + strand at 2294.021 kb on + strand at 2294.021 kb on + strand at 2294.021 kb on + strand at 2294.021 kb on + strand at 2294.021 kb on + strand at 2294.021 kb on + strand at 2294.021 kb on + strand at 2294.021 kb on + strand at 2294.022 kb on - strand at 2294.022 kb on - strand at 2294.022 kb on - strand at 2294.022 kb on - strand at 2294.022 kb on - strand at 2294.031 kb on - strand at 2294.167 kb on + strand at 2294.211 kb on + strand at 2294.212 kb on - strand at 2294.458 kb on + strand, within Pf1N1B4_2009 at 2294.459 kb on - strand, within Pf1N1B4_2009 at 2294.459 kb on - strand, within Pf1N1B4_2009 at 2294.531 kb on + strand, within Pf1N1B4_2009 at 2294.532 kb on - strand, within Pf1N1B4_2009 at 2294.532 kb on - strand, within Pf1N1B4_2009 at 2294.532 kb on - strand, within Pf1N1B4_2009 at 2294.532 kb on - strand, within Pf1N1B4_2009 at 2294.585 kb on + strand, within Pf1N1B4_2009 at 2294.586 kb on - strand, within Pf1N1B4_2009 at 2294.840 kb on + strand, within Pf1N1B4_2009 at 2294.840 kb on + strand, within Pf1N1B4_2009 at 2294.840 kb on + strand, within Pf1N1B4_2009 at 2294.841 kb on - strand, within Pf1N1B4_2009 at 2294.841 kb on - strand, within Pf1N1B4_2009 at 2294.981 kb on + strand, within Pf1N1B4_2009 at 2294.982 kb on - strand, within Pf1N1B4_2009 at 2294.999 kb on + strand, within Pf1N1B4_2009 at 2294.999 kb on + strand, within Pf1N1B4_2009 at 2294.999 kb on + strand, within Pf1N1B4_2009 at 2294.999 kb on + strand, within Pf1N1B4_2009 at 2295.000 kb on - strand, within Pf1N1B4_2009 at 2295.000 kb on - strand at 2295.000 kb on - strand, within Pf1N1B4_2009 at 2295.000 kb on - strand, within Pf1N1B4_2009 at 2295.096 kb on - strand at 2295.104 kb on - strand at 2295.104 kb on - strand at 2295.104 kb on - strand at 2295.104 kb on - strand at 2295.268 kb on - strand at 2295.364 kb on - strand at 2295.453 kb on - strand at 2295.453 kb on - strand at 2295.453 kb on - strand at 2295.453 kb on - strand at 2295.738 kb on + strand, within Pf1N1B4_2010 at 2296.115 kb on + strand, within Pf1N1B4_2010 at 2296.116 kb on - strand, within Pf1N1B4_2010 at 2296.116 kb on - strand, within Pf1N1B4_2010 at 2296.116 kb on - strand, within Pf1N1B4_2010
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 2,293,212 + Pf1N1B4_2008 0.32 -0.7 2,293,212 + Pf1N1B4_2008 0.32 +0.8 2,293,213 - Pf1N1B4_2008 0.32 +2.1 2,293,244 + Pf1N1B4_2008 0.36 -0.7 2,293,244 + Pf1N1B4_2008 0.36 -1.1 2,293,244 + Pf1N1B4_2008 0.36 -1.5 2,293,244 + Pf1N1B4_2008 0.36 +0.2 2,293,244 + Pf1N1B4_2008 0.36 -0.8 2,293,244 + Pf1N1B4_2008 0.36 -1.5 2,293,244 + Pf1N1B4_2008 0.36 -1.0 2,293,244 + Pf1N1B4_2008 0.36 +0.2 2,293,244 + Pf1N1B4_2008 0.36 -0.1 2,293,244 + Pf1N1B4_2008 0.36 -0.9 2,293,244 + Pf1N1B4_2008 0.36 +1.1 2,293,245 - Pf1N1B4_2008 0.36 -1.1 2,293,245 - Pf1N1B4_2008 0.36 -1.0 2,293,245 - Pf1N1B4_2008 0.36 -1.1 2,293,245 - Pf1N1B4_2008 0.36 -1.7 2,293,245 - Pf1N1B4_2008 0.36 +0.2 2,293,245 - Pf1N1B4_2008 0.36 -2.3 2,293,245 - Pf1N1B4_2008 0.36 -0.1 2,293,332 + Pf1N1B4_2008 0.46 -0.5 2,293,332 + Pf1N1B4_2008 0.46 -0.5 2,293,332 + Pf1N1B4_2008 0.46 +0.5 2,293,332 + Pf1N1B4_2008 0.46 -0.1 2,293,332 + Pf1N1B4_2008 0.46 -0.7 2,293,332 + Pf1N1B4_2008 0.46 -2.1 2,293,332 + Pf1N1B4_2008 0.46 -0.2 2,293,333 - Pf1N1B4_2008 0.46 -0.2 2,293,333 - Pf1N1B4_2008 0.46 -0.5 2,293,333 - Pf1N1B4_2008 0.46 +0.5 2,293,333 - Pf1N1B4_2008 0.46 -1.8 2,293,333 - Pf1N1B4_2008 0.46 -1.8 2,293,333 - Pf1N1B4_2008 0.46 -0.8 2,293,390 + Pf1N1B4_2008 0.53 -0.9 2,293,390 + Pf1N1B4_2008 0.53 -0.5 2,293,390 + Pf1N1B4_2008 0.53 -1.8 2,293,410 + Pf1N1B4_2008 0.55 -1.1 2,293,411 - Pf1N1B4_2008 0.55 -0.5 2,293,475 + Pf1N1B4_2008 0.63 -0.1 2,293,475 + Pf1N1B4_2008 0.63 -1.1 2,293,475 + Pf1N1B4_2008 0.63 -0.7 2,293,475 + Pf1N1B4_2008 0.63 -2.1 2,293,475 + Pf1N1B4_2008 0.63 +0.5 2,293,475 + Pf1N1B4_2008 0.63 -1.1 2,293,475 + Pf1N1B4_2008 0.63 +0.9 2,293,476 - Pf1N1B4_2008 0.63 -0.5 2,293,476 - Pf1N1B4_2008 0.63 +2.8 2,293,476 - Pf1N1B4_2008 0.63 -0.2 2,293,632 + Pf1N1B4_2008 0.81 -0.9 2,293,632 + Pf1N1B4_2008 0.81 -2.8 2,293,632 + Pf1N1B4_2008 0.81 +0.5 2,293,632 + Pf1N1B4_2008 0.81 +0.5 2,293,632 + Pf1N1B4_2008 0.81 -0.5 2,293,632 + Pf1N1B4_2008 0.81 -0.5 2,293,632 + Pf1N1B4_2008 0.81 -1.2 2,293,632 + Pf1N1B4_2008 0.81 -1.8 2,293,632 + Pf1N1B4_2008 0.81 -1.7 2,293,633 - Pf1N1B4_2008 0.81 +0.9 2,293,633 - Pf1N1B4_2008 0.81 -2.1 2,293,633 - +1.1 2,293,633 - Pf1N1B4_2008 0.81 -1.3 2,293,633 - Pf1N1B4_2008 0.81 -0.5 2,293,634 + Pf1N1B4_2008 0.81 +1.3 2,293,635 - Pf1N1B4_2008 0.81 -0.5 2,293,666 + Pf1N1B4_2008 0.85 +1.1 2,293,946 - -1.5 2,293,946 - -0.9 2,293,946 - -0.1 2,294,014 + -0.5 2,294,014 + +0.5 2,294,014 + -1.1 2,294,014 + +0.0 2,294,014 + +1.1 2,294,014 + -0.5 2,294,014 + -0.1 2,294,014 + +0.5 2,294,015 - -0.7 2,294,015 - +0.1 2,294,015 - -0.5 2,294,015 - +1.5 2,294,015 - -1.8 2,294,015 - -0.5 2,294,015 - +1.5 2,294,015 - -0.5 2,294,015 - +0.5 2,294,021 + -1.2 2,294,021 + +0.5 2,294,021 + -0.1 2,294,021 + -1.1 2,294,021 + -0.5 2,294,021 + +1.5 2,294,021 + +0.2 2,294,021 + +0.1 2,294,021 + +1.5 2,294,022 - +0.5 2,294,022 - +0.8 2,294,022 - +0.6 2,294,022 - -0.5 2,294,022 - -1.1 2,294,031 - +1.1 2,294,167 + -0.1 2,294,211 + +0.2 2,294,212 - +1.1 2,294,458 + Pf1N1B4_2009 0.27 -2.1 2,294,459 - Pf1N1B4_2009 0.27 -3.3 2,294,459 - Pf1N1B4_2009 0.27 -2.1 2,294,531 + Pf1N1B4_2009 0.35 -2.3 2,294,532 - Pf1N1B4_2009 0.35 -0.5 2,294,532 - Pf1N1B4_2009 0.35 -2.1 2,294,532 - Pf1N1B4_2009 0.35 -1.5 2,294,532 - Pf1N1B4_2009 0.35 -0.5 2,294,585 + Pf1N1B4_2009 0.40 -2.8 2,294,586 - Pf1N1B4_2009 0.40 -1.1 2,294,840 + Pf1N1B4_2009 0.67 +0.5 2,294,840 + Pf1N1B4_2009 0.67 -1.1 2,294,840 + Pf1N1B4_2009 0.67 -1.5 2,294,841 - Pf1N1B4_2009 0.67 -1.1 2,294,841 - Pf1N1B4_2009 0.67 -1.1 2,294,981 + Pf1N1B4_2009 0.82 -1.5 2,294,982 - Pf1N1B4_2009 0.82 -2.8 2,294,999 + Pf1N1B4_2009 0.84 -1.1 2,294,999 + Pf1N1B4_2009 0.84 -1.5 2,294,999 + Pf1N1B4_2009 0.84 -1.1 2,294,999 + Pf1N1B4_2009 0.84 -1.1 2,295,000 - Pf1N1B4_2009 0.84 -1.5 2,295,000 - -0.5 2,295,000 - Pf1N1B4_2009 0.84 -3.1 2,295,000 - Pf1N1B4_2009 0.84 -1.1 2,295,096 - -0.5 2,295,104 - -1.1 2,295,104 - -1.1 2,295,104 - -1.8 2,295,104 - -2.3 2,295,268 - -2.5 2,295,364 - +1.1 2,295,453 - -1.5 2,295,453 - -1.5 2,295,453 - -3.8 2,295,453 - +0.5 2,295,738 + Pf1N1B4_2010 0.27 -2.7 2,296,115 + Pf1N1B4_2010 0.53 -2.1 2,296,116 - Pf1N1B4_2010 0.53 -1.1 2,296,116 - Pf1N1B4_2010 0.53 -2.3 2,296,116 - Pf1N1B4_2010 0.53 -0.5
Or see this region's nucleotide sequence