Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2004 and Pf1N1B4_2005 are separated by 312 nucleotides Pf1N1B4_2005 and Pf1N1B4_2006 overlap by 48 nucleotides Pf1N1B4_2006 and Pf1N1B4_2007 are separated by 114 nucleotides
Pf1N1B4_2004 - putative membrane protein, at 2,286,260 to 2,287,135
_2004
Pf1N1B4_2005 - ATP-dependent DNA helicase UvrD/PcrA, at 2,287,448 to 2,289,631
_2005
Pf1N1B4_2006 - hypothetical protein, at 2,289,584 to 2,289,775
_2006
Pf1N1B4_2007 - Sensory box/GGDEF family protein, at 2,289,890 to 2,292,763
_2007
Position (kb)
2287
2288
2289
2290 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2286.456 kb on + strand, within Pf1N1B4_2004 at 2286.457 kb on - strand, within Pf1N1B4_2004 at 2286.457 kb on - strand, within Pf1N1B4_2004 at 2286.749 kb on - strand, within Pf1N1B4_2004 at 2287.121 kb on - strand at 2287.234 kb on + strand at 2287.234 kb on + strand at 2287.234 kb on + strand at 2287.235 kb on - strand at 2287.235 kb on - strand at 2287.235 kb on - strand at 2287.259 kb on + strand at 2287.260 kb on - strand at 2287.270 kb on - strand at 2287.270 kb on - strand at 2287.270 kb on - strand at 2287.283 kb on - strand at 2287.283 kb on - strand at 2287.283 kb on - strand at 2287.283 kb on - strand at 2287.296 kb on - strand at 2287.296 kb on - strand at 2287.296 kb on - strand at 2287.305 kb on - strand at 2287.305 kb on - strand at 2287.305 kb on - strand at 2287.334 kb on + strand at 2287.334 kb on + strand at 2287.334 kb on + strand at 2287.334 kb on + strand at 2287.335 kb on - strand at 2287.335 kb on - strand at 2287.335 kb on - strand at 2287.335 kb on - strand at 2287.335 kb on - strand at 2287.335 kb on - strand at 2287.449 kb on + strand at 2287.449 kb on + strand at 2287.449 kb on + strand at 2287.449 kb on + strand at 2287.450 kb on - strand at 2287.450 kb on - strand at 2287.450 kb on - strand at 2287.450 kb on - strand at 2287.785 kb on + strand, within Pf1N1B4_2005 at 2288.632 kb on - strand, within Pf1N1B4_2005 at 2288.725 kb on + strand, within Pf1N1B4_2005 at 2289.096 kb on + strand, within Pf1N1B4_2005 at 2289.106 kb on - strand at 2289.151 kb on + strand, within Pf1N1B4_2005 at 2289.642 kb on + strand, within Pf1N1B4_2006 at 2289.642 kb on + strand, within Pf1N1B4_2006 at 2289.643 kb on - strand, within Pf1N1B4_2006 at 2289.643 kb on - strand, within Pf1N1B4_2006 at 2289.643 kb on - strand, within Pf1N1B4_2006 at 2289.643 kb on - strand, within Pf1N1B4_2006 at 2289.765 kb on + strand at 2289.766 kb on - strand at 2289.766 kb on - strand at 2289.795 kb on + strand at 2289.833 kb on + strand at 2289.833 kb on + strand at 2289.833 kb on + strand at 2289.833 kb on + strand at 2289.833 kb on + strand at 2289.833 kb on + strand at 2289.834 kb on - strand at 2289.834 kb on - strand at 2289.834 kb on - strand at 2289.834 kb on - strand at 2289.868 kb on + strand at 2289.868 kb on + strand at 2289.868 kb on + strand at 2289.868 kb on + strand at 2289.869 kb on - strand at 2289.869 kb on - strand at 2289.869 kb on - strand at 2289.869 kb on - strand at 2289.869 kb on - strand at 2289.870 kb on + strand at 2289.870 kb on + strand at 2289.870 kb on + strand at 2289.870 kb on + strand at 2289.870 kb on + strand at 2289.870 kb on + strand at 2289.871 kb on - strand at 2289.871 kb on - strand at 2289.871 kb on - strand at 2289.871 kb on - strand at 2289.890 kb on - strand at 2289.950 kb on + strand at 2289.951 kb on - strand at 2289.951 kb on - strand at 2289.989 kb on + strand at 2290.070 kb on + strand at 2290.071 kb on - strand at 2290.071 kb on - strand at 2290.071 kb on - strand at 2290.071 kb on - strand at 2290.071 kb on - strand at 2290.071 kb on - strand at 2290.189 kb on + strand, within Pf1N1B4_2007 at 2290.189 kb on + strand, within Pf1N1B4_2007 at 2290.189 kb on + strand, within Pf1N1B4_2007 at 2290.189 kb on + strand, within Pf1N1B4_2007 at 2290.189 kb on + strand, within Pf1N1B4_2007 at 2290.189 kb on + strand, within Pf1N1B4_2007 at 2290.189 kb on + strand, within Pf1N1B4_2007 at 2290.189 kb on + strand, within Pf1N1B4_2007 at 2290.190 kb on - strand, within Pf1N1B4_2007 at 2290.190 kb on - strand, within Pf1N1B4_2007 at 2290.190 kb on - strand, within Pf1N1B4_2007 at 2290.190 kb on - strand, within Pf1N1B4_2007 at 2290.190 kb on - strand, within Pf1N1B4_2007 at 2290.190 kb on - strand, within Pf1N1B4_2007 at 2290.190 kb on - strand, within Pf1N1B4_2007 at 2290.328 kb on + strand, within Pf1N1B4_2007 at 2290.329 kb on - strand, within Pf1N1B4_2007 at 2290.360 kb on + strand, within Pf1N1B4_2007 at 2290.360 kb on + strand, within Pf1N1B4_2007 at 2290.361 kb on - strand, within Pf1N1B4_2007 at 2290.446 kb on + strand, within Pf1N1B4_2007 at 2290.447 kb on - strand, within Pf1N1B4_2007 at 2290.447 kb on - strand, within Pf1N1B4_2007 at 2290.629 kb on + strand, within Pf1N1B4_2007 at 2290.629 kb on + strand, within Pf1N1B4_2007 at 2290.629 kb on + strand, within Pf1N1B4_2007 at 2290.629 kb on + strand, within Pf1N1B4_2007 at 2290.629 kb on + strand, within Pf1N1B4_2007 at 2290.630 kb on - strand, within Pf1N1B4_2007 at 2290.630 kb on - strand, within Pf1N1B4_2007
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 2,286,456 + Pf1N1B4_2004 0.22 -0.9 2,286,457 - Pf1N1B4_2004 0.22 -1.3 2,286,457 - Pf1N1B4_2004 0.22 -0.5 2,286,749 - Pf1N1B4_2004 0.56 -1.8 2,287,121 - -0.8 2,287,234 + -0.5 2,287,234 + +0.5 2,287,234 + -0.8 2,287,235 - +1.1 2,287,235 - -2.7 2,287,235 - -0.5 2,287,259 + -1.3 2,287,260 - -0.1 2,287,270 - +0.5 2,287,270 - -0.8 2,287,270 - -0.1 2,287,283 - -0.7 2,287,283 - +0.3 2,287,283 - -2.7 2,287,283 - +1.0 2,287,296 - -0.1 2,287,296 - -0.5 2,287,296 - -2.3 2,287,305 - +0.1 2,287,305 - -0.6 2,287,305 - -0.5 2,287,334 + -0.1 2,287,334 + +0.1 2,287,334 + -1.3 2,287,334 + -0.5 2,287,335 - -0.1 2,287,335 - -0.3 2,287,335 - +0.5 2,287,335 - -0.6 2,287,335 - +0.2 2,287,335 - +1.2 2,287,449 + -0.2 2,287,449 + -0.5 2,287,449 + -0.8 2,287,449 + -1.5 2,287,450 - -0.4 2,287,450 - -0.5 2,287,450 - -0.2 2,287,450 - -2.3 2,287,785 + Pf1N1B4_2005 0.15 -1.5 2,288,632 - Pf1N1B4_2005 0.54 -2.5 2,288,725 + Pf1N1B4_2005 0.58 +1.2 2,289,096 + Pf1N1B4_2005 0.75 -0.5 2,289,106 - -0.5 2,289,151 + Pf1N1B4_2005 0.78 -1.8 2,289,642 + Pf1N1B4_2006 0.30 -0.5 2,289,642 + Pf1N1B4_2006 0.30 -0.9 2,289,643 - Pf1N1B4_2006 0.31 -1.1 2,289,643 - Pf1N1B4_2006 0.31 -2.1 2,289,643 - Pf1N1B4_2006 0.31 -0.5 2,289,643 - Pf1N1B4_2006 0.31 +0.5 2,289,765 + -0.1 2,289,766 - -1.1 2,289,766 - -1.1 2,289,795 + -1.3 2,289,833 + -1.3 2,289,833 + -1.1 2,289,833 + +0.1 2,289,833 + +0.5 2,289,833 + +0.5 2,289,833 + -1.2 2,289,834 - +0.2 2,289,834 - -0.4 2,289,834 - +0.2 2,289,834 - +1.3 2,289,868 + -0.6 2,289,868 + +1.5 2,289,868 + -1.5 2,289,868 + -1.1 2,289,869 - -0.1 2,289,869 - -0.4 2,289,869 - -1.3 2,289,869 - +2.5 2,289,869 - -1.5 2,289,870 + -0.7 2,289,870 + -1.1 2,289,870 + -0.5 2,289,870 + -0.3 2,289,870 + -1.1 2,289,870 + -0.7 2,289,871 - -0.1 2,289,871 - +1.8 2,289,871 - -2.1 2,289,871 - -0.1 2,289,890 - -1.1 2,289,950 + -0.5 2,289,951 - -0.1 2,289,951 - +0.4 2,289,989 + +0.1 2,290,070 + -0.5 2,290,071 - +0.5 2,290,071 - +1.5 2,290,071 - +0.5 2,290,071 - -0.5 2,290,071 - -0.9 2,290,071 - +0.5 2,290,189 + Pf1N1B4_2007 0.10 -0.1 2,290,189 + Pf1N1B4_2007 0.10 +0.5 2,290,189 + Pf1N1B4_2007 0.10 -2.2 2,290,189 + Pf1N1B4_2007 0.10 -0.3 2,290,189 + Pf1N1B4_2007 0.10 +0.5 2,290,189 + Pf1N1B4_2007 0.10 -0.7 2,290,189 + Pf1N1B4_2007 0.10 +0.8 2,290,189 + Pf1N1B4_2007 0.10 -0.5 2,290,190 - Pf1N1B4_2007 0.10 -1.5 2,290,190 - Pf1N1B4_2007 0.10 +0.7 2,290,190 - Pf1N1B4_2007 0.10 -0.1 2,290,190 - Pf1N1B4_2007 0.10 -0.1 2,290,190 - Pf1N1B4_2007 0.10 -1.1 2,290,190 - Pf1N1B4_2007 0.10 +1.5 2,290,190 - Pf1N1B4_2007 0.10 +0.5 2,290,328 + Pf1N1B4_2007 0.15 +0.5 2,290,329 - Pf1N1B4_2007 0.15 +1.5 2,290,360 + Pf1N1B4_2007 0.16 -2.3 2,290,360 + Pf1N1B4_2007 0.16 -0.2 2,290,361 - Pf1N1B4_2007 0.16 +0.5 2,290,446 + Pf1N1B4_2007 0.19 +1.2 2,290,447 - Pf1N1B4_2007 0.19 -2.3 2,290,447 - Pf1N1B4_2007 0.19 -1.5 2,290,629 + Pf1N1B4_2007 0.26 +1.5 2,290,629 + Pf1N1B4_2007 0.26 +0.6 2,290,629 + Pf1N1B4_2007 0.26 +0.2 2,290,629 + Pf1N1B4_2007 0.26 +0.2 2,290,629 + Pf1N1B4_2007 0.26 -1.1 2,290,630 - Pf1N1B4_2007 0.26 +0.5 2,290,630 - Pf1N1B4_2007 0.26 -2.3
Or see this region's nucleotide sequence