Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2002 and Pf1N1B4_2003 are separated by 22 nucleotides Pf1N1B4_2003 and Pf1N1B4_2004 are separated by 136 nucleotides Pf1N1B4_2004 and Pf1N1B4_2005 are separated by 312 nucleotides
Pf1N1B4_2002 - putative Glutathione-regulated potassium-efflux system protein KefB, at 2,283,930 to 2,285,693
_2002
Pf1N1B4_2003 - FIG00956226: hypothetical protein, at 2,285,716 to 2,286,123
_2003
Pf1N1B4_2004 - putative membrane protein, at 2,286,260 to 2,287,135
_2004
Pf1N1B4_2005 - ATP-dependent DNA helicase UvrD/PcrA, at 2,287,448 to 2,289,631
_2005
Position (kb)
2286
2287
2288 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2285.287 kb on - strand, within Pf1N1B4_2002 at 2285.384 kb on + strand, within Pf1N1B4_2002 at 2285.384 kb on + strand, within Pf1N1B4_2002 at 2285.385 kb on - strand at 2285.385 kb on - strand, within Pf1N1B4_2002 at 2285.385 kb on - strand, within Pf1N1B4_2002 at 2285.578 kb on + strand at 2285.579 kb on - strand at 2285.630 kb on + strand at 2285.713 kb on - strand at 2285.713 kb on - strand at 2285.752 kb on + strand at 2285.831 kb on + strand, within Pf1N1B4_2003 at 2285.834 kb on + strand, within Pf1N1B4_2003 at 2285.834 kb on + strand, within Pf1N1B4_2003 at 2285.834 kb on + strand, within Pf1N1B4_2003 at 2285.834 kb on + strand, within Pf1N1B4_2003 at 2285.835 kb on - strand, within Pf1N1B4_2003 at 2285.835 kb on - strand, within Pf1N1B4_2003 at 2285.835 kb on - strand, within Pf1N1B4_2003 at 2285.835 kb on - strand, within Pf1N1B4_2003 at 2285.835 kb on - strand, within Pf1N1B4_2003 at 2285.835 kb on - strand, within Pf1N1B4_2003 at 2285.915 kb on + strand, within Pf1N1B4_2003 at 2285.915 kb on + strand, within Pf1N1B4_2003 at 2285.916 kb on - strand, within Pf1N1B4_2003 at 2285.916 kb on - strand, within Pf1N1B4_2003 at 2286.155 kb on + strand at 2286.156 kb on - strand at 2286.169 kb on + strand at 2286.169 kb on + strand at 2286.171 kb on + strand at 2286.171 kb on + strand at 2286.171 kb on + strand at 2286.171 kb on + strand at 2286.171 kb on + strand at 2286.171 kb on + strand at 2286.172 kb on - strand at 2286.172 kb on - strand at 2286.172 kb on - strand at 2286.172 kb on - strand at 2286.172 kb on - strand at 2286.172 kb on - strand at 2286.172 kb on - strand at 2286.176 kb on + strand at 2286.176 kb on + strand at 2286.176 kb on + strand at 2286.176 kb on + strand at 2286.176 kb on + strand at 2286.177 kb on - strand at 2286.177 kb on - strand at 2286.186 kb on + strand at 2286.186 kb on + strand at 2286.186 kb on + strand at 2286.186 kb on + strand at 2286.186 kb on + strand at 2286.187 kb on - strand at 2286.187 kb on - strand at 2286.187 kb on - strand at 2286.187 kb on - strand at 2286.187 kb on - strand at 2286.187 kb on - strand at 2286.187 kb on - strand at 2286.187 kb on - strand at 2286.187 kb on - strand at 2286.187 kb on - strand at 2286.187 kb on - strand at 2286.203 kb on + strand at 2286.231 kb on + strand at 2286.231 kb on + strand at 2286.235 kb on - strand at 2286.438 kb on - strand, within Pf1N1B4_2004 at 2286.438 kb on - strand, within Pf1N1B4_2004 at 2286.456 kb on + strand, within Pf1N1B4_2004 at 2286.457 kb on - strand, within Pf1N1B4_2004 at 2286.457 kb on - strand, within Pf1N1B4_2004 at 2286.749 kb on - strand, within Pf1N1B4_2004 at 2287.121 kb on - strand at 2287.234 kb on + strand at 2287.234 kb on + strand at 2287.234 kb on + strand at 2287.235 kb on - strand at 2287.235 kb on - strand at 2287.235 kb on - strand at 2287.259 kb on + strand at 2287.260 kb on - strand at 2287.270 kb on - strand at 2287.270 kb on - strand at 2287.270 kb on - strand at 2287.283 kb on - strand at 2287.283 kb on - strand at 2287.283 kb on - strand at 2287.283 kb on - strand at 2287.296 kb on - strand at 2287.296 kb on - strand at 2287.296 kb on - strand at 2287.305 kb on - strand at 2287.305 kb on - strand at 2287.305 kb on - strand at 2287.334 kb on + strand at 2287.334 kb on + strand at 2287.334 kb on + strand at 2287.334 kb on + strand at 2287.335 kb on - strand at 2287.335 kb on - strand at 2287.335 kb on - strand at 2287.335 kb on - strand at 2287.335 kb on - strand at 2287.335 kb on - strand at 2287.449 kb on + strand at 2287.449 kb on + strand at 2287.449 kb on + strand at 2287.449 kb on + strand at 2287.450 kb on - strand at 2287.450 kb on - strand at 2287.450 kb on - strand at 2287.450 kb on - strand at 2287.785 kb on + strand, within Pf1N1B4_2005
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 2,285,287 - Pf1N1B4_2002 0.77 -2.1 2,285,384 + Pf1N1B4_2002 0.82 -1.1 2,285,384 + Pf1N1B4_2002 0.82 -2.1 2,285,385 - +0.5 2,285,385 - Pf1N1B4_2002 0.82 -0.8 2,285,385 - Pf1N1B4_2002 0.82 -0.3 2,285,578 + -1.1 2,285,579 - -0.5 2,285,630 + -0.5 2,285,713 - -0.1 2,285,713 - +1.8 2,285,752 + -0.5 2,285,831 + Pf1N1B4_2003 0.28 -0.1 2,285,834 + Pf1N1B4_2003 0.29 -1.1 2,285,834 + Pf1N1B4_2003 0.29 -0.8 2,285,834 + Pf1N1B4_2003 0.29 -0.3 2,285,834 + Pf1N1B4_2003 0.29 +0.5 2,285,835 - Pf1N1B4_2003 0.29 +0.5 2,285,835 - Pf1N1B4_2003 0.29 +1.2 2,285,835 - Pf1N1B4_2003 0.29 -0.5 2,285,835 - Pf1N1B4_2003 0.29 +0.9 2,285,835 - Pf1N1B4_2003 0.29 -1.5 2,285,835 - Pf1N1B4_2003 0.29 +1.1 2,285,915 + Pf1N1B4_2003 0.49 -0.4 2,285,915 + Pf1N1B4_2003 0.49 -0.5 2,285,916 - Pf1N1B4_2003 0.49 -1.2 2,285,916 - Pf1N1B4_2003 0.49 -0.1 2,286,155 + +0.5 2,286,156 - -0.5 2,286,169 + +1.1 2,286,169 + -1.5 2,286,171 + -0.2 2,286,171 + -0.5 2,286,171 + -0.5 2,286,171 + +1.5 2,286,171 + -1.8 2,286,171 + -2.3 2,286,172 - +0.7 2,286,172 - -0.1 2,286,172 - +2.3 2,286,172 - +0.2 2,286,172 - +1.5 2,286,172 - +2.1 2,286,172 - +2.7 2,286,176 + -0.5 2,286,176 + +0.5 2,286,176 + +2.5 2,286,176 + +0.5 2,286,176 + +0.5 2,286,177 - +1.5 2,286,177 - +0.6 2,286,186 + -1.1 2,286,186 + -0.8 2,286,186 + +0.5 2,286,186 + -0.1 2,286,186 + -0.8 2,286,187 - -0.2 2,286,187 - -0.7 2,286,187 - +0.2 2,286,187 - -0.7 2,286,187 - -1.5 2,286,187 - -0.5 2,286,187 - -0.5 2,286,187 - +0.5 2,286,187 - +1.1 2,286,187 - +0.2 2,286,187 - +0.6 2,286,203 + +0.5 2,286,231 + -0.5 2,286,231 + -0.9 2,286,235 - -0.5 2,286,438 - Pf1N1B4_2004 0.20 -1.1 2,286,438 - Pf1N1B4_2004 0.20 +1.1 2,286,456 + Pf1N1B4_2004 0.22 -0.9 2,286,457 - Pf1N1B4_2004 0.22 -1.3 2,286,457 - Pf1N1B4_2004 0.22 -0.5 2,286,749 - Pf1N1B4_2004 0.56 -1.8 2,287,121 - -0.8 2,287,234 + -0.5 2,287,234 + +0.5 2,287,234 + -0.8 2,287,235 - +1.1 2,287,235 - -2.7 2,287,235 - -0.5 2,287,259 + -1.3 2,287,260 - -0.1 2,287,270 - +0.5 2,287,270 - -0.8 2,287,270 - -0.1 2,287,283 - -0.7 2,287,283 - +0.3 2,287,283 - -2.7 2,287,283 - +1.0 2,287,296 - -0.1 2,287,296 - -0.5 2,287,296 - -2.3 2,287,305 - +0.1 2,287,305 - -0.6 2,287,305 - -0.5 2,287,334 + -0.1 2,287,334 + +0.1 2,287,334 + -1.3 2,287,334 + -0.5 2,287,335 - -0.1 2,287,335 - -0.3 2,287,335 - +0.5 2,287,335 - -0.6 2,287,335 - +0.2 2,287,335 - +1.2 2,287,449 + -0.2 2,287,449 + -0.5 2,287,449 + -0.8 2,287,449 + -1.5 2,287,450 - -0.4 2,287,450 - -0.5 2,287,450 - -0.2 2,287,450 - -2.3 2,287,785 + Pf1N1B4_2005 0.15 -1.5
Or see this region's nucleotide sequence