Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1830 and Pf1N1B4_1831 overlap by 1 nucleotides Pf1N1B4_1831 and Pf1N1B4_1832 are separated by 112 nucleotides
Pf1N1B4_1830 - Tryptophan synthase alpha chain (EC 4.2.1.20), at 2,106,499 to 2,107,311
_1830
Pf1N1B4_1831 - Tryptophan synthase beta chain (EC 4.2.1.20), at 2,107,311 to 2,108,540
_1831
Pf1N1B4_1832 - Glycine cleavage system transcriptional activator, at 2,108,653 to 2,109,546
_1832
Position (kb)
2107
2108
2109 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 2106.379 kb on + strand at 2106.379 kb on + strand at 2106.380 kb on - strand at 2106.380 kb on - strand at 2106.380 kb on - strand at 2106.380 kb on - strand at 2106.423 kb on + strand at 2106.424 kb on - strand at 2106.424 kb on - strand at 2106.451 kb on + strand at 2106.451 kb on + strand at 2106.452 kb on - strand at 2106.452 kb on - strand at 2106.452 kb on - strand at 2106.452 kb on - strand at 2106.452 kb on - strand at 2106.471 kb on + strand at 2106.471 kb on + strand at 2106.471 kb on + strand at 2106.472 kb on - strand at 2106.494 kb on + strand at 2106.494 kb on + strand at 2106.494 kb on + strand at 2106.494 kb on + strand at 2106.494 kb on + strand at 2106.495 kb on - strand at 2106.516 kb on + strand at 2106.517 kb on - strand at 2106.517 kb on - strand at 2106.517 kb on - strand at 2106.520 kb on + strand at 2106.520 kb on + strand at 2106.554 kb on - strand at 2106.667 kb on - strand, within Pf1N1B4_1830 at 2106.667 kb on - strand, within Pf1N1B4_1830 at 2106.667 kb on - strand, within Pf1N1B4_1830 at 2106.667 kb on - strand, within Pf1N1B4_1830 at 2106.782 kb on - strand, within Pf1N1B4_1830 at 2106.786 kb on - strand, within Pf1N1B4_1830 at 2106.786 kb on - strand, within Pf1N1B4_1830 at 2106.786 kb on - strand, within Pf1N1B4_1830 at 2106.786 kb on - strand, within Pf1N1B4_1830 at 2106.786 kb on - strand, within Pf1N1B4_1830 at 2106.786 kb on - strand, within Pf1N1B4_1830 at 2106.786 kb on - strand, within Pf1N1B4_1830 at 2106.786 kb on - strand, within Pf1N1B4_1830 at 2106.786 kb on - strand, within Pf1N1B4_1830 at 2106.786 kb on - strand, within Pf1N1B4_1830 at 2106.786 kb on - strand, within Pf1N1B4_1830 at 2106.786 kb on - strand, within Pf1N1B4_1830 at 2106.789 kb on - strand, within Pf1N1B4_1830 at 2106.789 kb on - strand, within Pf1N1B4_1830 at 2106.789 kb on - strand, within Pf1N1B4_1830 at 2106.789 kb on - strand, within Pf1N1B4_1830 at 2106.789 kb on - strand, within Pf1N1B4_1830 at 2106.789 kb on - strand, within Pf1N1B4_1830 at 2106.815 kb on - strand, within Pf1N1B4_1830 at 2106.815 kb on - strand, within Pf1N1B4_1830 at 2106.815 kb on - strand, within Pf1N1B4_1830 at 2106.907 kb on - strand, within Pf1N1B4_1830 at 2106.907 kb on - strand, within Pf1N1B4_1830 at 2106.907 kb on - strand, within Pf1N1B4_1830 at 2106.907 kb on - strand, within Pf1N1B4_1830 at 2106.987 kb on - strand, within Pf1N1B4_1830 at 2106.987 kb on - strand, within Pf1N1B4_1830 at 2106.987 kb on - strand, within Pf1N1B4_1830 at 2106.987 kb on - strand, within Pf1N1B4_1830 at 2106.987 kb on - strand, within Pf1N1B4_1830 at 2106.987 kb on - strand, within Pf1N1B4_1830 at 2106.987 kb on - strand, within Pf1N1B4_1830 at 2106.987 kb on - strand, within Pf1N1B4_1830 at 2106.987 kb on - strand, within Pf1N1B4_1830 at 2107.008 kb on - strand, within Pf1N1B4_1830 at 2107.008 kb on - strand, within Pf1N1B4_1830 at 2107.008 kb on - strand, within Pf1N1B4_1830 at 2107.172 kb on - strand, within Pf1N1B4_1830 at 2107.172 kb on - strand, within Pf1N1B4_1830 at 2107.224 kb on - strand, within Pf1N1B4_1830 at 2107.224 kb on - strand, within Pf1N1B4_1830 at 2107.224 kb on - strand, within Pf1N1B4_1830 at 2107.224 kb on - strand, within Pf1N1B4_1830 at 2107.224 kb on - strand, within Pf1N1B4_1830 at 2107.224 kb on - strand, within Pf1N1B4_1830 at 2107.482 kb on - strand, within Pf1N1B4_1831 at 2107.482 kb on - strand, within Pf1N1B4_1831 at 2107.546 kb on + strand, within Pf1N1B4_1831 at 2107.547 kb on - strand, within Pf1N1B4_1831 at 2107.547 kb on - strand, within Pf1N1B4_1831 at 2107.547 kb on - strand, within Pf1N1B4_1831 at 2107.547 kb on - strand, within Pf1N1B4_1831 at 2107.547 kb on - strand, within Pf1N1B4_1831 at 2107.547 kb on - strand, within Pf1N1B4_1831 at 2107.547 kb on - strand, within Pf1N1B4_1831 at 2107.601 kb on - strand, within Pf1N1B4_1831 at 2107.961 kb on - strand, within Pf1N1B4_1831 at 2107.961 kb on - strand, within Pf1N1B4_1831 at 2107.961 kb on - strand, within Pf1N1B4_1831 at 2107.961 kb on - strand, within Pf1N1B4_1831 at 2108.019 kb on - strand, within Pf1N1B4_1831 at 2108.019 kb on - strand, within Pf1N1B4_1831 at 2108.120 kb on - strand, within Pf1N1B4_1831 at 2108.120 kb on - strand, within Pf1N1B4_1831 at 2108.120 kb on - strand, within Pf1N1B4_1831 at 2108.120 kb on - strand, within Pf1N1B4_1831 at 2108.120 kb on - strand, within Pf1N1B4_1831 at 2108.120 kb on - strand, within Pf1N1B4_1831 at 2108.120 kb on - strand, within Pf1N1B4_1831 at 2108.120 kb on - strand, within Pf1N1B4_1831 at 2108.120 kb on - strand, within Pf1N1B4_1831 at 2108.120 kb on - strand, within Pf1N1B4_1831 at 2108.120 kb on - strand, within Pf1N1B4_1831 at 2108.120 kb on - strand, within Pf1N1B4_1831 at 2108.120 kb on - strand, within Pf1N1B4_1831 at 2108.120 kb on - strand, within Pf1N1B4_1831 at 2108.120 kb on - strand, within Pf1N1B4_1831 at 2108.120 kb on - strand, within Pf1N1B4_1831 at 2108.120 kb on - strand, within Pf1N1B4_1831 at 2108.120 kb on - strand, within Pf1N1B4_1831 at 2108.120 kb on - strand, within Pf1N1B4_1831 at 2108.120 kb on - strand, within Pf1N1B4_1831 at 2108.120 kb on - strand, within Pf1N1B4_1831 at 2108.120 kb on - strand, within Pf1N1B4_1831 at 2108.120 kb on - strand, within Pf1N1B4_1831 at 2108.120 kb on - strand, within Pf1N1B4_1831 at 2108.120 kb on - strand, within Pf1N1B4_1831 at 2108.120 kb on - strand, within Pf1N1B4_1831 at 2108.120 kb on - strand, within Pf1N1B4_1831 at 2108.120 kb on - strand, within Pf1N1B4_1831 at 2108.120 kb on - strand, within Pf1N1B4_1831 at 2108.120 kb on - strand, within Pf1N1B4_1831 at 2108.120 kb on - strand, within Pf1N1B4_1831 at 2108.120 kb on - strand, within Pf1N1B4_1831 at 2108.297 kb on - strand, within Pf1N1B4_1831 at 2108.297 kb on - strand, within Pf1N1B4_1831 at 2108.297 kb on - strand, within Pf1N1B4_1831 at 2108.297 kb on - strand, within Pf1N1B4_1831 at 2108.297 kb on - strand, within Pf1N1B4_1831 at 2108.342 kb on - strand, within Pf1N1B4_1831 at 2108.342 kb on - strand, within Pf1N1B4_1831 at 2108.342 kb on - strand, within Pf1N1B4_1831 at 2108.342 kb on - strand, within Pf1N1B4_1831 at 2108.342 kb on - strand, within Pf1N1B4_1831 at 2108.342 kb on - strand at 2108.342 kb on - strand, within Pf1N1B4_1831 at 2108.342 kb on - strand, within Pf1N1B4_1831 at 2108.342 kb on - strand, within Pf1N1B4_1831 at 2108.342 kb on - strand, within Pf1N1B4_1831 at 2108.366 kb on - strand, within Pf1N1B4_1831 at 2108.366 kb on - strand, within Pf1N1B4_1831 at 2108.366 kb on - strand, within Pf1N1B4_1831 at 2108.366 kb on - strand, within Pf1N1B4_1831 at 2108.366 kb on - strand, within Pf1N1B4_1831 at 2108.366 kb on - strand, within Pf1N1B4_1831 at 2108.366 kb on - strand, within Pf1N1B4_1831 at 2108.366 kb on - strand, within Pf1N1B4_1831 at 2108.366 kb on - strand, within Pf1N1B4_1831 at 2108.381 kb on - strand, within Pf1N1B4_1831 at 2108.381 kb on - strand, within Pf1N1B4_1831 at 2108.381 kb on - strand, within Pf1N1B4_1831 at 2108.426 kb on - strand at 2108.529 kb on - strand at 2108.529 kb on - strand at 2108.556 kb on + strand at 2108.556 kb on + strand at 2108.556 kb on + strand at 2108.556 kb on + strand at 2108.556 kb on + strand at 2108.557 kb on - strand at 2108.557 kb on - strand at 2108.557 kb on - strand at 2108.585 kb on + strand at 2109.089 kb on + strand, within Pf1N1B4_1832 at 2109.089 kb on + strand, within Pf1N1B4_1832 at 2109.090 kb on - strand, within Pf1N1B4_1832 at 2109.090 kb on - strand, within Pf1N1B4_1832 at 2109.127 kb on + strand, within Pf1N1B4_1832 at 2109.127 kb on + strand, within Pf1N1B4_1832 at 2109.128 kb on - strand, within Pf1N1B4_1832 at 2109.307 kb on + strand, within Pf1N1B4_1832 at 2109.307 kb on + strand, within Pf1N1B4_1832 at 2109.307 kb on + strand, within Pf1N1B4_1832 at 2109.307 kb on + strand, within Pf1N1B4_1832 at 2109.308 kb on - strand, within Pf1N1B4_1832 at 2109.308 kb on - strand, within Pf1N1B4_1832 at 2109.308 kb on - strand, within Pf1N1B4_1832 at 2109.308 kb on - strand, within Pf1N1B4_1832 at 2109.308 kb on - strand, within Pf1N1B4_1832 at 2109.308 kb on - strand, within Pf1N1B4_1832 at 2109.308 kb on - strand, within Pf1N1B4_1832 at 2109.308 kb on - strand, within Pf1N1B4_1832 at 2109.308 kb on - strand, within Pf1N1B4_1832 at 2109.332 kb on - strand, within Pf1N1B4_1832 at 2109.332 kb on - strand at 2109.332 kb on - strand, within Pf1N1B4_1832 at 2109.332 kb on - strand, within Pf1N1B4_1832 at 2109.334 kb on + strand, within Pf1N1B4_1832 at 2109.334 kb on + strand, within Pf1N1B4_1832 at 2109.334 kb on + strand, within Pf1N1B4_1832 at 2109.335 kb on - strand, within Pf1N1B4_1832 at 2109.519 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 2,106,379 + -0.5 2,106,379 + +0.1 2,106,380 - -2.1 2,106,380 - -1.5 2,106,380 - -1.5 2,106,380 - -1.1 2,106,423 + -0.1 2,106,424 - +1.5 2,106,424 - -0.5 2,106,451 + -0.6 2,106,451 + -1.1 2,106,452 - +0.5 2,106,452 - -0.7 2,106,452 - -0.5 2,106,452 - +0.5 2,106,452 - +0.5 2,106,471 + -1.5 2,106,471 + -1.1 2,106,471 + -0.5 2,106,472 - -0.4 2,106,494 + +0.5 2,106,494 + +0.5 2,106,494 + -2.1 2,106,494 + +1.5 2,106,494 + -1.3 2,106,495 - -0.1 2,106,516 + +0.8 2,106,517 - -0.6 2,106,517 - -0.8 2,106,517 - -0.5 2,106,520 + +1.2 2,106,520 + +0.5 2,106,554 - -1.1 2,106,667 - Pf1N1B4_1830 0.21 -1.5 2,106,667 - Pf1N1B4_1830 0.21 -2.1 2,106,667 - Pf1N1B4_1830 0.21 -2.3 2,106,667 - Pf1N1B4_1830 0.21 -1.4 2,106,782 - Pf1N1B4_1830 0.35 -2.1 2,106,786 - Pf1N1B4_1830 0.35 -1.8 2,106,786 - Pf1N1B4_1830 0.35 -2.7 2,106,786 - Pf1N1B4_1830 0.35 -2.5 2,106,786 - Pf1N1B4_1830 0.35 -2.7 2,106,786 - Pf1N1B4_1830 0.35 -1.5 2,106,786 - Pf1N1B4_1830 0.35 -3.2 2,106,786 - Pf1N1B4_1830 0.35 -1.8 2,106,786 - Pf1N1B4_1830 0.35 -2.3 2,106,786 - Pf1N1B4_1830 0.35 -3.1 2,106,786 - Pf1N1B4_1830 0.35 -1.8 2,106,786 - Pf1N1B4_1830 0.35 -0.5 2,106,786 - Pf1N1B4_1830 0.35 -1.5 2,106,789 - Pf1N1B4_1830 0.36 -2.3 2,106,789 - Pf1N1B4_1830 0.36 -2.1 2,106,789 - Pf1N1B4_1830 0.36 -2.1 2,106,789 - Pf1N1B4_1830 0.36 -2.3 2,106,789 - Pf1N1B4_1830 0.36 -0.1 2,106,789 - Pf1N1B4_1830 0.36 -1.5 2,106,815 - Pf1N1B4_1830 0.39 -2.5 2,106,815 - Pf1N1B4_1830 0.39 -2.1 2,106,815 - Pf1N1B4_1830 0.39 -2.1 2,106,907 - Pf1N1B4_1830 0.50 -2.7 2,106,907 - Pf1N1B4_1830 0.50 -1.3 2,106,907 - Pf1N1B4_1830 0.50 -0.5 2,106,907 - Pf1N1B4_1830 0.50 -2.7 2,106,987 - Pf1N1B4_1830 0.60 -2.9 2,106,987 - Pf1N1B4_1830 0.60 +0.2 2,106,987 - Pf1N1B4_1830 0.60 -0.5 2,106,987 - Pf1N1B4_1830 0.60 -1.5 2,106,987 - Pf1N1B4_1830 0.60 -0.8 2,106,987 - Pf1N1B4_1830 0.60 -1.1 2,106,987 - Pf1N1B4_1830 0.60 -2.5 2,106,987 - Pf1N1B4_1830 0.60 -1.8 2,106,987 - Pf1N1B4_1830 0.60 -0.5 2,107,008 - Pf1N1B4_1830 0.63 -0.3 2,107,008 - Pf1N1B4_1830 0.63 -2.1 2,107,008 - Pf1N1B4_1830 0.63 -1.5 2,107,172 - Pf1N1B4_1830 0.83 -1.8 2,107,172 - Pf1N1B4_1830 0.83 -3.6 2,107,224 - Pf1N1B4_1830 0.89 -2.1 2,107,224 - Pf1N1B4_1830 0.89 -2.9 2,107,224 - Pf1N1B4_1830 0.89 -1.1 2,107,224 - Pf1N1B4_1830 0.89 -2.5 2,107,224 - Pf1N1B4_1830 0.89 -1.1 2,107,224 - Pf1N1B4_1830 0.89 -2.3 2,107,482 - Pf1N1B4_1831 0.14 -1.5 2,107,482 - Pf1N1B4_1831 0.14 -1.5 2,107,546 + Pf1N1B4_1831 0.19 -1.5 2,107,547 - Pf1N1B4_1831 0.19 -0.5 2,107,547 - Pf1N1B4_1831 0.19 -2.5 2,107,547 - Pf1N1B4_1831 0.19 -2.1 2,107,547 - Pf1N1B4_1831 0.19 -2.3 2,107,547 - Pf1N1B4_1831 0.19 -1.5 2,107,547 - Pf1N1B4_1831 0.19 -1.8 2,107,547 - Pf1N1B4_1831 0.19 -1.8 2,107,601 - Pf1N1B4_1831 0.24 -1.8 2,107,961 - Pf1N1B4_1831 0.53 +0.5 2,107,961 - Pf1N1B4_1831 0.53 -1.8 2,107,961 - Pf1N1B4_1831 0.53 -2.1 2,107,961 - Pf1N1B4_1831 0.53 -0.5 2,108,019 - Pf1N1B4_1831 0.58 -0.5 2,108,019 - Pf1N1B4_1831 0.58 -3.1 2,108,120 - Pf1N1B4_1831 0.66 -3.1 2,108,120 - Pf1N1B4_1831 0.66 -2.7 2,108,120 - Pf1N1B4_1831 0.66 -1.8 2,108,120 - Pf1N1B4_1831 0.66 -1.8 2,108,120 - Pf1N1B4_1831 0.66 -2.5 2,108,120 - Pf1N1B4_1831 0.66 -3.9 2,108,120 - Pf1N1B4_1831 0.66 -2.7 2,108,120 - Pf1N1B4_1831 0.66 +0.5 2,108,120 - Pf1N1B4_1831 0.66 -1.1 2,108,120 - Pf1N1B4_1831 0.66 -2.1 2,108,120 - Pf1N1B4_1831 0.66 -1.8 2,108,120 - Pf1N1B4_1831 0.66 -2.3 2,108,120 - Pf1N1B4_1831 0.66 -1.8 2,108,120 - Pf1N1B4_1831 0.66 -1.5 2,108,120 - Pf1N1B4_1831 0.66 -2.7 2,108,120 - Pf1N1B4_1831 0.66 +0.5 2,108,120 - Pf1N1B4_1831 0.66 -5.0 2,108,120 - Pf1N1B4_1831 0.66 -2.7 2,108,120 - Pf1N1B4_1831 0.66 -2.9 2,108,120 - Pf1N1B4_1831 0.66 -1.1 2,108,120 - Pf1N1B4_1831 0.66 -2.7 2,108,120 - Pf1N1B4_1831 0.66 -1.5 2,108,120 - Pf1N1B4_1831 0.66 -2.3 2,108,120 - Pf1N1B4_1831 0.66 -2.1 2,108,120 - Pf1N1B4_1831 0.66 -1.8 2,108,120 - Pf1N1B4_1831 0.66 -0.5 2,108,120 - Pf1N1B4_1831 0.66 -0.5 2,108,120 - Pf1N1B4_1831 0.66 -0.5 2,108,120 - Pf1N1B4_1831 0.66 -2.1 2,108,120 - Pf1N1B4_1831 0.66 -2.3 2,108,120 - Pf1N1B4_1831 0.66 -1.9 2,108,120 - Pf1N1B4_1831 0.66 -1.1 2,108,297 - Pf1N1B4_1831 0.80 -0.7 2,108,297 - Pf1N1B4_1831 0.80 -1.1 2,108,297 - Pf1N1B4_1831 0.80 -1.1 2,108,297 - Pf1N1B4_1831 0.80 -3.3 2,108,297 - Pf1N1B4_1831 0.80 -3.9 2,108,342 - Pf1N1B4_1831 0.84 -2.1 2,108,342 - Pf1N1B4_1831 0.84 -2.7 2,108,342 - Pf1N1B4_1831 0.84 -2.5 2,108,342 - Pf1N1B4_1831 0.84 -4.1 2,108,342 - Pf1N1B4_1831 0.84 -1.1 2,108,342 - -0.5 2,108,342 - Pf1N1B4_1831 0.84 -1.8 2,108,342 - Pf1N1B4_1831 0.84 -2.1 2,108,342 - Pf1N1B4_1831 0.84 -0.5 2,108,342 - Pf1N1B4_1831 0.84 -3.5 2,108,366 - Pf1N1B4_1831 0.86 -2.9 2,108,366 - Pf1N1B4_1831 0.86 -2.1 2,108,366 - Pf1N1B4_1831 0.86 -0.5 2,108,366 - Pf1N1B4_1831 0.86 -1.8 2,108,366 - Pf1N1B4_1831 0.86 -2.0 2,108,366 - Pf1N1B4_1831 0.86 -1.5 2,108,366 - Pf1N1B4_1831 0.86 -1.8 2,108,366 - Pf1N1B4_1831 0.86 -1.8 2,108,366 - Pf1N1B4_1831 0.86 -1.1 2,108,381 - Pf1N1B4_1831 0.87 -0.5 2,108,381 - Pf1N1B4_1831 0.87 -1.5 2,108,381 - Pf1N1B4_1831 0.87 -2.5 2,108,426 - -2.3 2,108,529 - -2.5 2,108,529 - -1.5 2,108,556 + -2.8 2,108,556 + -1.5 2,108,556 + +0.5 2,108,556 + -0.2 2,108,556 + -2.3 2,108,557 - +0.5 2,108,557 - +0.3 2,108,557 - -0.8 2,108,585 + -2.1 2,109,089 + Pf1N1B4_1832 0.49 -0.6 2,109,089 + Pf1N1B4_1832 0.49 -0.2 2,109,090 - Pf1N1B4_1832 0.49 -0.9 2,109,090 - Pf1N1B4_1832 0.49 -1.1 2,109,127 + Pf1N1B4_1832 0.53 -0.8 2,109,127 + Pf1N1B4_1832 0.53 -0.5 2,109,128 - Pf1N1B4_1832 0.53 +0.1 2,109,307 + Pf1N1B4_1832 0.73 +0.5 2,109,307 + Pf1N1B4_1832 0.73 +1.0 2,109,307 + Pf1N1B4_1832 0.73 +1.3 2,109,307 + Pf1N1B4_1832 0.73 -1.1 2,109,308 - Pf1N1B4_1832 0.73 +0.9 2,109,308 - Pf1N1B4_1832 0.73 -1.7 2,109,308 - Pf1N1B4_1832 0.73 +0.2 2,109,308 - Pf1N1B4_1832 0.73 -0.5 2,109,308 - Pf1N1B4_1832 0.73 +0.0 2,109,308 - Pf1N1B4_1832 0.73 -0.9 2,109,308 - Pf1N1B4_1832 0.73 +0.5 2,109,308 - Pf1N1B4_1832 0.73 -0.2 2,109,308 - Pf1N1B4_1832 0.73 -0.1 2,109,332 - Pf1N1B4_1832 0.76 -0.5 2,109,332 - -1.5 2,109,332 - Pf1N1B4_1832 0.76 -0.1 2,109,332 - Pf1N1B4_1832 0.76 +0.5 2,109,334 + Pf1N1B4_1832 0.76 +0.3 2,109,334 + Pf1N1B4_1832 0.76 +0.1 2,109,334 + Pf1N1B4_1832 0.76 +0.1 2,109,335 - Pf1N1B4_1832 0.76 -0.5 2,109,519 + -1.1
Or see this region's nucleotide sequence