Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1729 and Pf1N1B4_1730 are separated by 147 nucleotides Pf1N1B4_1730 and Pf1N1B4_1731 are separated by 316 nucleotides
Pf1N1B4_1729 - GGDEF domain protein, at 1,975,137 to 1,977,056
_1729
Pf1N1B4_1730 - Fatty acid desaturase (EC 1.14.19.1); Delta-9 fatty acid desaturase (EC 1.14.19.1), at 1,977,204 to 1,978,388
_1730
Pf1N1B4_1731 - Sensory box/GGDEF family protein, at 1,978,705 to 1,979,694
_1731
Position (kb)
1977
1978
1979 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1976.486 kb on - strand, within Pf1N1B4_1729 at 1976.486 kb on - strand, within Pf1N1B4_1729 at 1976.486 kb on - strand, within Pf1N1B4_1729 at 1976.623 kb on + strand, within Pf1N1B4_1729 at 1976.744 kb on + strand, within Pf1N1B4_1729 at 1976.744 kb on + strand, within Pf1N1B4_1729 at 1976.744 kb on + strand, within Pf1N1B4_1729 at 1976.744 kb on + strand, within Pf1N1B4_1729 at 1976.744 kb on + strand, within Pf1N1B4_1729 at 1976.744 kb on + strand, within Pf1N1B4_1729 at 1976.744 kb on + strand, within Pf1N1B4_1729 at 1976.744 kb on + strand, within Pf1N1B4_1729 at 1976.744 kb on + strand, within Pf1N1B4_1729 at 1976.745 kb on - strand, within Pf1N1B4_1729 at 1976.745 kb on - strand, within Pf1N1B4_1729 at 1976.745 kb on - strand, within Pf1N1B4_1729 at 1976.819 kb on + strand, within Pf1N1B4_1729 at 1976.819 kb on + strand, within Pf1N1B4_1729 at 1976.911 kb on + strand at 1976.951 kb on + strand at 1976.970 kb on + strand at 1976.970 kb on + strand at 1976.971 kb on - strand at 1976.971 kb on - strand at 1976.974 kb on + strand at 1976.975 kb on - strand at 1976.981 kb on + strand at 1976.982 kb on - strand at 1977.013 kb on + strand at 1977.014 kb on - strand at 1977.043 kb on + strand at 1977.043 kb on + strand at 1977.057 kb on + strand at 1977.057 kb on + strand at 1977.057 kb on + strand at 1977.057 kb on + strand at 1977.057 kb on + strand at 1977.057 kb on + strand at 1977.057 kb on + strand at 1977.057 kb on + strand at 1977.058 kb on - strand at 1977.058 kb on - strand at 1977.093 kb on + strand at 1977.093 kb on + strand at 1977.093 kb on + strand at 1977.098 kb on + strand at 1977.098 kb on + strand at 1977.098 kb on + strand at 1977.098 kb on + strand at 1977.098 kb on + strand at 1977.098 kb on + strand at 1977.120 kb on + strand at 1977.120 kb on + strand at 1977.120 kb on + strand at 1977.120 kb on + strand at 1977.120 kb on + strand at 1977.120 kb on + strand at 1977.120 kb on + strand at 1977.120 kb on + strand at 1977.121 kb on - strand at 1977.142 kb on + strand at 1977.142 kb on + strand at 1977.142 kb on + strand at 1977.142 kb on + strand at 1977.142 kb on + strand at 1977.143 kb on - strand at 1977.143 kb on - strand at 1977.143 kb on - strand at 1977.153 kb on + strand at 1977.153 kb on + strand at 1977.160 kb on + strand at 1977.196 kb on + strand at 1977.198 kb on + strand at 1977.199 kb on - strand at 1977.199 kb on - strand at 1977.199 kb on - strand at 1977.199 kb on - strand at 1977.203 kb on + strand at 1977.203 kb on + strand at 1977.203 kb on + strand at 1977.203 kb on + strand at 1977.203 kb on + strand at 1977.211 kb on - strand at 1977.211 kb on - strand at 1977.259 kb on + strand at 1977.310 kb on - strand at 1977.549 kb on + strand, within Pf1N1B4_1730 at 1977.549 kb on + strand, within Pf1N1B4_1730 at 1977.550 kb on - strand, within Pf1N1B4_1730 at 1977.591 kb on + strand, within Pf1N1B4_1730 at 1977.821 kb on + strand, within Pf1N1B4_1730 at 1977.821 kb on + strand, within Pf1N1B4_1730 at 1977.842 kb on + strand, within Pf1N1B4_1730 at 1977.843 kb on - strand, within Pf1N1B4_1730 at 1978.211 kb on + strand, within Pf1N1B4_1730 at 1978.363 kb on - strand at 1978.363 kb on - strand at 1978.484 kb on + strand at 1978.484 kb on + strand at 1978.484 kb on + strand at 1978.484 kb on + strand at 1978.484 kb on + strand at 1978.559 kb on + strand at 1978.677 kb on - strand at 1978.710 kb on + strand at 1978.713 kb on + strand at 1978.713 kb on + strand at 1978.714 kb on - strand at 1978.714 kb on - strand at 1978.714 kb on - strand at 1978.721 kb on + strand at 1978.722 kb on - strand at 1978.867 kb on + strand, within Pf1N1B4_1731 at 1978.867 kb on + strand, within Pf1N1B4_1731 at 1978.867 kb on + strand, within Pf1N1B4_1731 at 1978.867 kb on + strand, within Pf1N1B4_1731 at 1978.867 kb on + strand, within Pf1N1B4_1731 at 1978.868 kb on - strand, within Pf1N1B4_1731 at 1978.868 kb on - strand, within Pf1N1B4_1731 at 1978.868 kb on - strand, within Pf1N1B4_1731 at 1978.894 kb on + strand, within Pf1N1B4_1731 at 1978.894 kb on + strand, within Pf1N1B4_1731 at 1978.894 kb on + strand, within Pf1N1B4_1731 at 1978.894 kb on + strand, within Pf1N1B4_1731 at 1978.894 kb on + strand, within Pf1N1B4_1731 at 1978.895 kb on - strand, within Pf1N1B4_1731 at 1978.909 kb on + strand, within Pf1N1B4_1731 at 1978.910 kb on - strand, within Pf1N1B4_1731 at 1978.954 kb on + strand, within Pf1N1B4_1731 at 1978.954 kb on + strand, within Pf1N1B4_1731 at 1978.955 kb on - strand, within Pf1N1B4_1731 at 1978.955 kb on - strand, within Pf1N1B4_1731 at 1978.955 kb on - strand, within Pf1N1B4_1731 at 1978.996 kb on + strand, within Pf1N1B4_1731 at 1978.997 kb on - strand, within Pf1N1B4_1731 at 1979.205 kb on - strand, within Pf1N1B4_1731 at 1979.205 kb on - strand, within Pf1N1B4_1731 at 1979.205 kb on - strand, within Pf1N1B4_1731 at 1979.354 kb on - strand, within Pf1N1B4_1731
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 1,976,486 - Pf1N1B4_1729 0.70 -0.1 1,976,486 - Pf1N1B4_1729 0.70 +0.5 1,976,486 - Pf1N1B4_1729 0.70 +0.9 1,976,623 + Pf1N1B4_1729 0.77 -1.8 1,976,744 + Pf1N1B4_1729 0.84 +1.5 1,976,744 + Pf1N1B4_1729 0.84 +1.2 1,976,744 + Pf1N1B4_1729 0.84 -1.1 1,976,744 + Pf1N1B4_1729 0.84 -1.8 1,976,744 + Pf1N1B4_1729 0.84 -1.1 1,976,744 + Pf1N1B4_1729 0.84 -2.2 1,976,744 + Pf1N1B4_1729 0.84 +1.2 1,976,744 + Pf1N1B4_1729 0.84 +1.5 1,976,744 + Pf1N1B4_1729 0.84 +2.1 1,976,745 - Pf1N1B4_1729 0.84 +1.8 1,976,745 - Pf1N1B4_1729 0.84 -0.2 1,976,745 - Pf1N1B4_1729 0.84 -0.2 1,976,819 + Pf1N1B4_1729 0.88 +1.1 1,976,819 + Pf1N1B4_1729 0.88 -0.1 1,976,911 + +1.6 1,976,951 + +0.8 1,976,970 + +0.8 1,976,970 + -0.5 1,976,971 - +0.5 1,976,971 - -0.5 1,976,974 + +0.5 1,976,975 - -1.5 1,976,981 + -0.4 1,976,982 - -1.8 1,977,013 + -1.2 1,977,014 - -0.3 1,977,043 + -0.4 1,977,043 + -0.3 1,977,057 + +0.8 1,977,057 + +0.8 1,977,057 + -0.5 1,977,057 + -0.6 1,977,057 + -0.5 1,977,057 + -2.7 1,977,057 + -1.3 1,977,057 + +0.5 1,977,058 - +0.5 1,977,058 - +1.5 1,977,093 + -1.1 1,977,093 + -0.6 1,977,093 + +0.5 1,977,098 + -0.9 1,977,098 + -0.4 1,977,098 + -0.1 1,977,098 + +2.8 1,977,098 + -0.1 1,977,098 + +1.2 1,977,120 + +1.1 1,977,120 + +2.2 1,977,120 + +0.7 1,977,120 + -0.4 1,977,120 + +0.2 1,977,120 + -0.1 1,977,120 + +0.5 1,977,120 + +0.2 1,977,121 - -0.8 1,977,142 + +0.9 1,977,142 + +0.5 1,977,142 + -0.5 1,977,142 + +0.0 1,977,142 + +0.3 1,977,143 - +1.1 1,977,143 - +0.8 1,977,143 - -1.5 1,977,153 + +0.5 1,977,153 + -0.5 1,977,160 + -1.8 1,977,196 + -0.5 1,977,198 + -1.1 1,977,199 - -1.1 1,977,199 - -1.1 1,977,199 - -1.5 1,977,199 - -0.1 1,977,203 + +0.1 1,977,203 + -0.5 1,977,203 + -0.1 1,977,203 + +0.5 1,977,203 + -0.5 1,977,211 - +0.7 1,977,211 - +0.5 1,977,259 + +1.2 1,977,310 - -0.7 1,977,549 + Pf1N1B4_1730 0.29 +0.1 1,977,549 + Pf1N1B4_1730 0.29 +0.1 1,977,550 - Pf1N1B4_1730 0.29 -1.1 1,977,591 + Pf1N1B4_1730 0.33 -1.8 1,977,821 + Pf1N1B4_1730 0.52 +0.5 1,977,821 + Pf1N1B4_1730 0.52 -1.5 1,977,842 + Pf1N1B4_1730 0.54 -0.8 1,977,843 - Pf1N1B4_1730 0.54 -2.7 1,978,211 + Pf1N1B4_1730 0.85 -2.1 1,978,363 - +0.5 1,978,363 - -0.2 1,978,484 + +0.5 1,978,484 + +0.3 1,978,484 + -0.1 1,978,484 + -0.5 1,978,484 + -0.8 1,978,559 + -0.5 1,978,677 - +0.5 1,978,710 + -1.5 1,978,713 + -1.1 1,978,713 + -0.6 1,978,714 - +1.5 1,978,714 - +0.7 1,978,714 - +2.1 1,978,721 + -0.1 1,978,722 - -0.0 1,978,867 + Pf1N1B4_1731 0.16 -0.7 1,978,867 + Pf1N1B4_1731 0.16 -0.7 1,978,867 + Pf1N1B4_1731 0.16 -1.1 1,978,867 + Pf1N1B4_1731 0.16 -0.5 1,978,867 + Pf1N1B4_1731 0.16 -0.8 1,978,868 - Pf1N1B4_1731 0.16 +0.1 1,978,868 - Pf1N1B4_1731 0.16 +0.5 1,978,868 - Pf1N1B4_1731 0.16 +2.5 1,978,894 + Pf1N1B4_1731 0.19 -0.5 1,978,894 + Pf1N1B4_1731 0.19 +0.1 1,978,894 + Pf1N1B4_1731 0.19 +1.8 1,978,894 + Pf1N1B4_1731 0.19 +0.1 1,978,894 + Pf1N1B4_1731 0.19 -0.5 1,978,895 - Pf1N1B4_1731 0.19 -1.1 1,978,909 + Pf1N1B4_1731 0.21 -0.5 1,978,910 - Pf1N1B4_1731 0.21 +2.8 1,978,954 + Pf1N1B4_1731 0.25 -0.3 1,978,954 + Pf1N1B4_1731 0.25 +1.1 1,978,955 - Pf1N1B4_1731 0.25 -1.5 1,978,955 - Pf1N1B4_1731 0.25 +0.5 1,978,955 - Pf1N1B4_1731 0.25 -0.3 1,978,996 + Pf1N1B4_1731 0.29 +1.5 1,978,997 - Pf1N1B4_1731 0.29 +1.1 1,979,205 - Pf1N1B4_1731 0.51 +0.2 1,979,205 - Pf1N1B4_1731 0.51 -1.2 1,979,205 - Pf1N1B4_1731 0.51 -0.8 1,979,354 - Pf1N1B4_1731 0.66 -1.1
Or see this region's nucleotide sequence