Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1678 and Pf1N1B4_1679 are separated by 281 nucleotides Pf1N1B4_1679 and Pf1N1B4_1680 overlap by 4 nucleotides
Pf1N1B4_1678 - Outer membrane component of tripartite multidrug resistance system, at 1,923,506 to 1,924,939
_1678
Pf1N1B4_1679 - Membrane-fusion protein, at 1,925,221 to 1,926,003
_1679
Pf1N1B4_1680 - Probable membrane-fusion protein, at 1,926,000 to 1,927,319
_1680
Position (kb)
1925
1926
1927 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1924.374 kb on + strand, within Pf1N1B4_1678 at 1924.374 kb on + strand, within Pf1N1B4_1678 at 1924.374 kb on + strand, within Pf1N1B4_1678 at 1924.374 kb on + strand, within Pf1N1B4_1678 at 1924.374 kb on + strand, within Pf1N1B4_1678 at 1924.374 kb on + strand, within Pf1N1B4_1678 at 1924.374 kb on + strand, within Pf1N1B4_1678 at 1924.374 kb on + strand, within Pf1N1B4_1678 at 1924.374 kb on + strand, within Pf1N1B4_1678 at 1924.374 kb on + strand, within Pf1N1B4_1678 at 1924.374 kb on + strand, within Pf1N1B4_1678 at 1924.374 kb on + strand, within Pf1N1B4_1678 at 1924.375 kb on - strand, within Pf1N1B4_1678 at 1924.375 kb on - strand, within Pf1N1B4_1678 at 1924.375 kb on - strand, within Pf1N1B4_1678 at 1924.375 kb on - strand, within Pf1N1B4_1678 at 1924.375 kb on - strand, within Pf1N1B4_1678 at 1924.375 kb on - strand, within Pf1N1B4_1678 at 1924.375 kb on - strand, within Pf1N1B4_1678 at 1924.375 kb on - strand, within Pf1N1B4_1678 at 1924.375 kb on - strand, within Pf1N1B4_1678 at 1924.375 kb on - strand, within Pf1N1B4_1678 at 1924.489 kb on - strand, within Pf1N1B4_1678 at 1924.622 kb on + strand, within Pf1N1B4_1678 at 1924.623 kb on - strand, within Pf1N1B4_1678 at 1924.623 kb on - strand, within Pf1N1B4_1678 at 1924.677 kb on - strand, within Pf1N1B4_1678 at 1924.760 kb on + strand, within Pf1N1B4_1678 at 1924.760 kb on + strand, within Pf1N1B4_1678 at 1924.760 kb on + strand, within Pf1N1B4_1678 at 1924.760 kb on + strand, within Pf1N1B4_1678 at 1924.760 kb on + strand, within Pf1N1B4_1678 at 1924.760 kb on + strand, within Pf1N1B4_1678 at 1924.761 kb on - strand, within Pf1N1B4_1678 at 1924.761 kb on - strand, within Pf1N1B4_1678 at 1924.761 kb on - strand, within Pf1N1B4_1678 at 1924.761 kb on - strand, within Pf1N1B4_1678 at 1924.761 kb on - strand, within Pf1N1B4_1678 at 1924.761 kb on - strand, within Pf1N1B4_1678 at 1924.877 kb on + strand at 1924.877 kb on + strand at 1924.877 kb on + strand at 1924.877 kb on + strand at 1924.877 kb on + strand at 1924.877 kb on + strand at 1924.877 kb on + strand at 1924.878 kb on - strand at 1924.878 kb on - strand at 1924.878 kb on - strand at 1924.878 kb on - strand at 1924.878 kb on - strand at 1924.878 kb on - strand at 1924.878 kb on - strand at 1924.878 kb on - strand at 1924.879 kb on + strand at 1924.880 kb on - strand at 1924.880 kb on - strand at 1924.911 kb on - strand at 1925.005 kb on + strand at 1925.016 kb on + strand at 1925.016 kb on + strand at 1925.017 kb on - strand at 1925.025 kb on + strand at 1925.025 kb on + strand at 1925.026 kb on - strand at 1925.026 kb on - strand at 1925.039 kb on + strand at 1925.039 kb on + strand at 1925.053 kb on + strand at 1925.053 kb on + strand at 1925.054 kb on - strand at 1925.054 kb on - strand at 1925.054 kb on - strand at 1925.054 kb on - strand at 1925.087 kb on + strand at 1925.087 kb on + strand at 1925.087 kb on + strand at 1925.087 kb on + strand at 1925.088 kb on - strand at 1925.109 kb on + strand at 1925.110 kb on - strand at 1925.110 kb on - strand at 1925.118 kb on + strand at 1925.118 kb on + strand at 1925.118 kb on + strand at 1925.118 kb on + strand at 1925.119 kb on - strand at 1925.119 kb on - strand at 1925.119 kb on - strand at 1925.188 kb on + strand at 1925.188 kb on + strand at 1925.189 kb on - strand at 1925.215 kb on - strand at 1925.221 kb on - strand at 1925.252 kb on + strand at 1925.252 kb on + strand at 1925.261 kb on + strand at 1925.262 kb on - strand at 1925.270 kb on + strand at 1925.270 kb on + strand at 1925.271 kb on - strand at 1925.271 kb on - strand at 1925.273 kb on + strand at 1925.273 kb on + strand at 1925.274 kb on - strand at 1925.274 kb on - strand at 1925.488 kb on + strand, within Pf1N1B4_1679 at 1925.489 kb on - strand, within Pf1N1B4_1679 at 1925.644 kb on + strand, within Pf1N1B4_1679 at 1925.651 kb on + strand, within Pf1N1B4_1679 at 1926.099 kb on + strand at 1926.099 kb on + strand at 1926.100 kb on - strand at 1926.462 kb on + strand, within Pf1N1B4_1680 at 1926.462 kb on + strand, within Pf1N1B4_1680 at 1926.462 kb on + strand, within Pf1N1B4_1680 at 1926.462 kb on + strand, within Pf1N1B4_1680 at 1926.462 kb on + strand, within Pf1N1B4_1680 at 1926.462 kb on + strand, within Pf1N1B4_1680 at 1926.462 kb on + strand, within Pf1N1B4_1680 at 1926.463 kb on - strand, within Pf1N1B4_1680 at 1926.463 kb on - strand, within Pf1N1B4_1680 at 1926.463 kb on - strand, within Pf1N1B4_1680 at 1926.463 kb on - strand, within Pf1N1B4_1680 at 1926.463 kb on - strand at 1926.463 kb on - strand, within Pf1N1B4_1680 at 1926.879 kb on + strand, within Pf1N1B4_1680 at 1926.880 kb on - strand, within Pf1N1B4_1680 at 1926.911 kb on - strand, within Pf1N1B4_1680
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 1,924,374 + Pf1N1B4_1678 0.61 -0.3 1,924,374 + Pf1N1B4_1678 0.61 -1.1 1,924,374 + Pf1N1B4_1678 0.61 -0.1 1,924,374 + Pf1N1B4_1678 0.61 +1.5 1,924,374 + Pf1N1B4_1678 0.61 -0.5 1,924,374 + Pf1N1B4_1678 0.61 +1.5 1,924,374 + Pf1N1B4_1678 0.61 -0.5 1,924,374 + Pf1N1B4_1678 0.61 +0.7 1,924,374 + Pf1N1B4_1678 0.61 -0.4 1,924,374 + Pf1N1B4_1678 0.61 -1.5 1,924,374 + Pf1N1B4_1678 0.61 +0.1 1,924,374 + Pf1N1B4_1678 0.61 -1.8 1,924,375 - Pf1N1B4_1678 0.61 -1.8 1,924,375 - Pf1N1B4_1678 0.61 -1.1 1,924,375 - Pf1N1B4_1678 0.61 -1.6 1,924,375 - Pf1N1B4_1678 0.61 -0.1 1,924,375 - Pf1N1B4_1678 0.61 -1.1 1,924,375 - Pf1N1B4_1678 0.61 +2.5 1,924,375 - Pf1N1B4_1678 0.61 +0.1 1,924,375 - Pf1N1B4_1678 0.61 +1.5 1,924,375 - Pf1N1B4_1678 0.61 -0.1 1,924,375 - Pf1N1B4_1678 0.61 -0.5 1,924,489 - Pf1N1B4_1678 0.69 -1.2 1,924,622 + Pf1N1B4_1678 0.78 +1.5 1,924,623 - Pf1N1B4_1678 0.78 +1.5 1,924,623 - Pf1N1B4_1678 0.78 -1.4 1,924,677 - Pf1N1B4_1678 0.82 -0.1 1,924,760 + Pf1N1B4_1678 0.87 -3.1 1,924,760 + Pf1N1B4_1678 0.87 -0.5 1,924,760 + Pf1N1B4_1678 0.87 +1.5 1,924,760 + Pf1N1B4_1678 0.87 +1.8 1,924,760 + Pf1N1B4_1678 0.87 +0.5 1,924,760 + Pf1N1B4_1678 0.87 -0.1 1,924,761 - Pf1N1B4_1678 0.88 -0.1 1,924,761 - Pf1N1B4_1678 0.88 +0.1 1,924,761 - Pf1N1B4_1678 0.88 +0.9 1,924,761 - Pf1N1B4_1678 0.88 +0.1 1,924,761 - Pf1N1B4_1678 0.88 +0.5 1,924,761 - Pf1N1B4_1678 0.88 -2.1 1,924,877 + +1.2 1,924,877 + -3.8 1,924,877 + -0.5 1,924,877 + +0.5 1,924,877 + -0.7 1,924,877 + -0.3 1,924,877 + -0.6 1,924,878 - +0.5 1,924,878 - -0.8 1,924,878 - -1.1 1,924,878 - -0.1 1,924,878 - -0.5 1,924,878 - -1.5 1,924,878 - +1.5 1,924,878 - +0.3 1,924,879 + -0.2 1,924,880 - -0.4 1,924,880 - +0.3 1,924,911 - -0.5 1,925,005 + -0.1 1,925,016 + -2.5 1,925,016 + -0.3 1,925,017 - +0.5 1,925,025 + +1.0 1,925,025 + -0.1 1,925,026 - +0.1 1,925,026 - -0.5 1,925,039 + -0.8 1,925,039 + +1.5 1,925,053 + -0.5 1,925,053 + +0.9 1,925,054 - -1.1 1,925,054 - -1.0 1,925,054 - -1.2 1,925,054 - -0.7 1,925,087 + -0.5 1,925,087 + -1.1 1,925,087 + -1.1 1,925,087 + +0.2 1,925,088 - -1.4 1,925,109 + -0.8 1,925,110 - -1.5 1,925,110 - -0.6 1,925,118 + -1.8 1,925,118 + -1.8 1,925,118 + +1.5 1,925,118 + -0.5 1,925,119 - -0.5 1,925,119 - +0.5 1,925,119 - +0.3 1,925,188 + -0.8 1,925,188 + +1.5 1,925,189 - +1.1 1,925,215 - +0.5 1,925,221 - -0.8 1,925,252 + +0.3 1,925,252 + -1.1 1,925,261 + -0.5 1,925,262 - +0.5 1,925,270 + -0.6 1,925,270 + +0.9 1,925,271 - -1.1 1,925,271 - +0.2 1,925,273 + -0.3 1,925,273 + -2.7 1,925,274 - +0.8 1,925,274 - +0.9 1,925,488 + Pf1N1B4_1679 0.34 +0.5 1,925,489 - Pf1N1B4_1679 0.34 +1.1 1,925,644 + Pf1N1B4_1679 0.54 -0.5 1,925,651 + Pf1N1B4_1679 0.55 +1.0 1,926,099 + -1.1 1,926,099 + +0.5 1,926,100 - +0.5 1,926,462 + Pf1N1B4_1680 0.35 -0.3 1,926,462 + Pf1N1B4_1680 0.35 -0.1 1,926,462 + Pf1N1B4_1680 0.35 -1.3 1,926,462 + Pf1N1B4_1680 0.35 +2.5 1,926,462 + Pf1N1B4_1680 0.35 -0.5 1,926,462 + Pf1N1B4_1680 0.35 +1.1 1,926,462 + Pf1N1B4_1680 0.35 +0.5 1,926,463 - Pf1N1B4_1680 0.35 +1.5 1,926,463 - Pf1N1B4_1680 0.35 -1.1 1,926,463 - Pf1N1B4_1680 0.35 -1.5 1,926,463 - Pf1N1B4_1680 0.35 -0.1 1,926,463 - +1.1 1,926,463 - Pf1N1B4_1680 0.35 +2.1 1,926,879 + Pf1N1B4_1680 0.67 +0.3 1,926,880 - Pf1N1B4_1680 0.67 -0.1 1,926,911 - Pf1N1B4_1680 0.69 +0.8
Or see this region's nucleotide sequence