Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1666 and Pf1N1B4_1667 are separated by 40 nucleotides Pf1N1B4_1667 and Pf1N1B4_1668 are separated by 117 nucleotides Pf1N1B4_1668 and Pf1N1B4_1669 overlap by 4 nucleotides Pf1N1B4_1669 and Pf1N1B4_1670 are separated by 88 nucleotides
Pf1N1B4_1666 - C4-dicarboxylate transport transcriptional regulatory protein, at 1,910,422 to 1,911,816
_1666
Pf1N1B4_1667 - Histone acetyltransferase HPA2 and related acetyltransferases, at 1,911,857 to 1,912,315
_1667
Pf1N1B4_1668 - 3'(2'),5'-bisphosphate nucleotidase (EC 3.1.3.7), at 1,912,433 to 1,913,266
_1668
Pf1N1B4_1669 - ADP compounds hydrolase NudE (EC 3.6.1.-), at 1,913,263 to 1,913,829
_1669
Pf1N1B4_1670 - FIG001957: putative hydrolase, at 1,913,918 to 1,914,580
_1670
Position (kb)
1912
1913
1914 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1911.515 kb on + strand, within Pf1N1B4_1666 at 1911.515 kb on + strand, within Pf1N1B4_1666 at 1911.515 kb on + strand, within Pf1N1B4_1666 at 1911.516 kb on - strand, within Pf1N1B4_1666 at 1911.819 kb on + strand at 1911.825 kb on - strand at 1912.022 kb on + strand, within Pf1N1B4_1667 at 1912.023 kb on - strand, within Pf1N1B4_1667 at 1912.023 kb on - strand, within Pf1N1B4_1667 at 1912.208 kb on + strand, within Pf1N1B4_1667 at 1912.208 kb on + strand, within Pf1N1B4_1667 at 1912.208 kb on + strand, within Pf1N1B4_1667 at 1912.208 kb on + strand, within Pf1N1B4_1667 at 1912.208 kb on + strand, within Pf1N1B4_1667 at 1912.209 kb on - strand at 1912.209 kb on - strand, within Pf1N1B4_1667 at 1912.298 kb on + strand at 1912.298 kb on + strand at 1912.298 kb on + strand at 1912.298 kb on + strand at 1912.298 kb on + strand at 1912.298 kb on + strand at 1912.298 kb on + strand at 1912.298 kb on + strand at 1912.298 kb on + strand at 1912.298 kb on + strand at 1912.298 kb on + strand at 1912.298 kb on + strand at 1912.298 kb on + strand at 1912.299 kb on - strand at 1912.299 kb on - strand at 1912.299 kb on - strand at 1912.422 kb on - strand at 1912.422 kb on - strand at 1912.422 kb on - strand at 1912.731 kb on - strand, within Pf1N1B4_1668 at 1912.837 kb on + strand, within Pf1N1B4_1668 at 1912.838 kb on - strand, within Pf1N1B4_1668 at 1912.875 kb on + strand, within Pf1N1B4_1668 at 1912.875 kb on + strand, within Pf1N1B4_1668 at 1912.876 kb on - strand, within Pf1N1B4_1668 at 1912.876 kb on - strand, within Pf1N1B4_1668 at 1912.972 kb on + strand, within Pf1N1B4_1668 at 1912.972 kb on + strand, within Pf1N1B4_1668 at 1912.972 kb on + strand, within Pf1N1B4_1668 at 1912.972 kb on + strand, within Pf1N1B4_1668 at 1912.972 kb on + strand, within Pf1N1B4_1668 at 1912.973 kb on - strand, within Pf1N1B4_1668 at 1912.973 kb on - strand, within Pf1N1B4_1668 at 1912.973 kb on - strand, within Pf1N1B4_1668 at 1912.973 kb on - strand, within Pf1N1B4_1668 at 1912.973 kb on - strand, within Pf1N1B4_1668 at 1913.293 kb on + strand at 1913.293 kb on + strand at 1913.293 kb on + strand at 1913.293 kb on + strand at 1913.293 kb on + strand at 1913.293 kb on + strand at 1913.294 kb on - strand at 1913.309 kb on + strand at 1913.309 kb on + strand at 1913.309 kb on + strand at 1913.309 kb on + strand at 1913.309 kb on + strand at 1913.310 kb on - strand at 1913.432 kb on + strand, within Pf1N1B4_1669 at 1913.432 kb on + strand, within Pf1N1B4_1669 at 1913.433 kb on - strand, within Pf1N1B4_1669 at 1913.474 kb on + strand, within Pf1N1B4_1669 at 1913.474 kb on + strand, within Pf1N1B4_1669 at 1913.475 kb on - strand, within Pf1N1B4_1669 at 1913.475 kb on - strand, within Pf1N1B4_1669 at 1913.475 kb on - strand, within Pf1N1B4_1669 at 1913.612 kb on + strand, within Pf1N1B4_1669 at 1913.612 kb on + strand, within Pf1N1B4_1669 at 1913.612 kb on + strand, within Pf1N1B4_1669 at 1913.612 kb on + strand, within Pf1N1B4_1669 at 1913.612 kb on + strand, within Pf1N1B4_1669 at 1913.612 kb on + strand, within Pf1N1B4_1669 at 1913.612 kb on + strand, within Pf1N1B4_1669 at 1913.613 kb on - strand, within Pf1N1B4_1669 at 1913.613 kb on - strand, within Pf1N1B4_1669 at 1913.633 kb on + strand, within Pf1N1B4_1669 at 1913.633 kb on + strand at 1913.723 kb on + strand, within Pf1N1B4_1669 at 1913.723 kb on + strand, within Pf1N1B4_1669 at 1913.723 kb on + strand, within Pf1N1B4_1669 at 1913.723 kb on + strand, within Pf1N1B4_1669 at 1913.723 kb on + strand, within Pf1N1B4_1669 at 1913.723 kb on + strand, within Pf1N1B4_1669 at 1913.723 kb on + strand, within Pf1N1B4_1669 at 1913.723 kb on + strand, within Pf1N1B4_1669 at 1913.723 kb on + strand, within Pf1N1B4_1669 at 1913.724 kb on - strand, within Pf1N1B4_1669 at 1913.724 kb on - strand, within Pf1N1B4_1669 at 1913.724 kb on - strand, within Pf1N1B4_1669 at 1913.724 kb on - strand, within Pf1N1B4_1669 at 1913.727 kb on + strand, within Pf1N1B4_1669 at 1913.727 kb on + strand, within Pf1N1B4_1669 at 1913.727 kb on + strand, within Pf1N1B4_1669 at 1913.727 kb on + strand, within Pf1N1B4_1669 at 1913.727 kb on + strand, within Pf1N1B4_1669 at 1913.728 kb on - strand, within Pf1N1B4_1669 at 1913.728 kb on - strand, within Pf1N1B4_1669 at 1913.728 kb on - strand, within Pf1N1B4_1669 at 1913.809 kb on + strand at 1913.809 kb on + strand at 1913.855 kb on + strand at 1913.856 kb on - strand at 1913.856 kb on - strand at 1913.858 kb on + strand at 1913.858 kb on + strand at 1913.858 kb on + strand at 1913.858 kb on + strand at 1913.858 kb on + strand at 1913.859 kb on - strand at 1913.859 kb on - strand at 1913.859 kb on - strand at 1913.859 kb on - strand at 1913.859 kb on - strand at 1913.859 kb on - strand at 1913.859 kb on - strand at 1913.900 kb on + strand at 1913.917 kb on + strand at 1913.917 kb on + strand at 1913.917 kb on + strand at 1913.917 kb on + strand at 1913.917 kb on + strand at 1913.917 kb on + strand at 1913.917 kb on + strand at 1913.917 kb on + strand at 1913.918 kb on - strand at 1913.918 kb on - strand at 1913.918 kb on - strand at 1913.918 kb on - strand at 1913.918 kb on - strand at 1913.918 kb on - strand at 1913.990 kb on + strand, within Pf1N1B4_1670 at 1913.991 kb on - strand, within Pf1N1B4_1670 at 1913.991 kb on - strand, within Pf1N1B4_1670 at 1914.075 kb on + strand, within Pf1N1B4_1670 at 1914.076 kb on - strand, within Pf1N1B4_1670 at 1914.117 kb on - strand, within Pf1N1B4_1670 at 1914.117 kb on - strand, within Pf1N1B4_1670 at 1914.117 kb on - strand, within Pf1N1B4_1670 at 1914.117 kb on - strand, within Pf1N1B4_1670 at 1914.252 kb on + strand, within Pf1N1B4_1670 at 1914.252 kb on + strand, within Pf1N1B4_1670 at 1914.253 kb on - strand, within Pf1N1B4_1670
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 1,911,515 + Pf1N1B4_1666 0.78 -0.1 1,911,515 + Pf1N1B4_1666 0.78 -0.1 1,911,515 + Pf1N1B4_1666 0.78 -1.9 1,911,516 - Pf1N1B4_1666 0.78 +0.5 1,911,819 + +0.9 1,911,825 - -0.1 1,912,022 + Pf1N1B4_1667 0.36 +0.1 1,912,023 - Pf1N1B4_1667 0.36 +0.5 1,912,023 - Pf1N1B4_1667 0.36 +0.7 1,912,208 + Pf1N1B4_1667 0.76 -0.8 1,912,208 + Pf1N1B4_1667 0.76 +0.5 1,912,208 + Pf1N1B4_1667 0.76 -1.9 1,912,208 + Pf1N1B4_1667 0.76 -1.3 1,912,208 + Pf1N1B4_1667 0.76 -0.3 1,912,209 - -0.5 1,912,209 - Pf1N1B4_1667 0.77 -0.1 1,912,298 + +0.5 1,912,298 + -0.5 1,912,298 + +2.8 1,912,298 + -0.5 1,912,298 + -0.3 1,912,298 + +0.5 1,912,298 + +0.1 1,912,298 + -0.7 1,912,298 + -1.1 1,912,298 + +0.5 1,912,298 + +0.7 1,912,298 + -0.7 1,912,298 + -0.0 1,912,299 - +1.5 1,912,299 - +1.0 1,912,299 - -0.1 1,912,422 - -0.1 1,912,422 - -1.5 1,912,422 - +0.5 1,912,731 - Pf1N1B4_1668 0.36 -0.5 1,912,837 + Pf1N1B4_1668 0.48 -2.1 1,912,838 - Pf1N1B4_1668 0.49 -1.1 1,912,875 + Pf1N1B4_1668 0.53 -2.7 1,912,875 + Pf1N1B4_1668 0.53 -1.1 1,912,876 - Pf1N1B4_1668 0.53 -2.5 1,912,876 - Pf1N1B4_1668 0.53 -3.3 1,912,972 + Pf1N1B4_1668 0.65 -0.5 1,912,972 + Pf1N1B4_1668 0.65 -3.0 1,912,972 + Pf1N1B4_1668 0.65 -2.1 1,912,972 + Pf1N1B4_1668 0.65 +0.5 1,912,972 + Pf1N1B4_1668 0.65 -0.5 1,912,973 - Pf1N1B4_1668 0.65 -1.5 1,912,973 - Pf1N1B4_1668 0.65 -0.5 1,912,973 - Pf1N1B4_1668 0.65 -2.7 1,912,973 - Pf1N1B4_1668 0.65 -1.9 1,912,973 - Pf1N1B4_1668 0.65 -0.8 1,913,293 + -0.1 1,913,293 + -1.4 1,913,293 + -0.5 1,913,293 + +0.1 1,913,293 + -1.1 1,913,293 + -1.1 1,913,294 - -0.2 1,913,309 + -0.1 1,913,309 + -0.5 1,913,309 + +1.8 1,913,309 + -0.9 1,913,309 + -0.5 1,913,310 - -1.5 1,913,432 + Pf1N1B4_1669 0.30 -1.1 1,913,432 + Pf1N1B4_1669 0.30 -0.9 1,913,433 - Pf1N1B4_1669 0.30 -0.3 1,913,474 + Pf1N1B4_1669 0.37 -0.3 1,913,474 + Pf1N1B4_1669 0.37 -1.0 1,913,475 - Pf1N1B4_1669 0.37 +0.3 1,913,475 - Pf1N1B4_1669 0.37 -0.8 1,913,475 - Pf1N1B4_1669 0.37 +0.5 1,913,612 + Pf1N1B4_1669 0.62 +0.0 1,913,612 + Pf1N1B4_1669 0.62 -1.3 1,913,612 + Pf1N1B4_1669 0.62 -1.3 1,913,612 + Pf1N1B4_1669 0.62 -1.5 1,913,612 + Pf1N1B4_1669 0.62 -0.4 1,913,612 + Pf1N1B4_1669 0.62 +0.0 1,913,612 + Pf1N1B4_1669 0.62 -0.5 1,913,613 - Pf1N1B4_1669 0.62 -0.4 1,913,613 - Pf1N1B4_1669 0.62 +0.5 1,913,633 + Pf1N1B4_1669 0.65 +2.0 1,913,633 + -0.5 1,913,723 + Pf1N1B4_1669 0.81 +0.5 1,913,723 + Pf1N1B4_1669 0.81 -2.7 1,913,723 + Pf1N1B4_1669 0.81 +0.3 1,913,723 + Pf1N1B4_1669 0.81 -0.2 1,913,723 + Pf1N1B4_1669 0.81 -0.5 1,913,723 + Pf1N1B4_1669 0.81 +0.5 1,913,723 + Pf1N1B4_1669 0.81 +1.5 1,913,723 + Pf1N1B4_1669 0.81 -0.0 1,913,723 + Pf1N1B4_1669 0.81 -2.4 1,913,724 - Pf1N1B4_1669 0.81 +0.5 1,913,724 - Pf1N1B4_1669 0.81 +0.4 1,913,724 - Pf1N1B4_1669 0.81 +0.5 1,913,724 - Pf1N1B4_1669 0.81 -0.1 1,913,727 + Pf1N1B4_1669 0.82 +1.0 1,913,727 + Pf1N1B4_1669 0.82 -1.0 1,913,727 + Pf1N1B4_1669 0.82 +1.0 1,913,727 + Pf1N1B4_1669 0.82 -0.3 1,913,727 + Pf1N1B4_1669 0.82 +0.1 1,913,728 - Pf1N1B4_1669 0.82 -0.9 1,913,728 - Pf1N1B4_1669 0.82 +1.2 1,913,728 - Pf1N1B4_1669 0.82 -1.5 1,913,809 + +0.2 1,913,809 + -1.2 1,913,855 + +2.0 1,913,856 - +1.9 1,913,856 - -0.3 1,913,858 + -1.5 1,913,858 + -2.1 1,913,858 + +0.5 1,913,858 + -1.5 1,913,858 + +0.5 1,913,859 - +2.0 1,913,859 - -0.1 1,913,859 - +1.0 1,913,859 - -0.5 1,913,859 - +0.8 1,913,859 - +0.0 1,913,859 - -0.3 1,913,900 + -0.5 1,913,917 + +0.5 1,913,917 + -2.1 1,913,917 + -0.9 1,913,917 + -1.0 1,913,917 + +2.0 1,913,917 + +0.1 1,913,917 + -1.1 1,913,917 + +1.0 1,913,918 - +0.5 1,913,918 - -1.5 1,913,918 - +0.9 1,913,918 - +0.0 1,913,918 - -0.1 1,913,918 - +1.0 1,913,990 + Pf1N1B4_1670 0.11 -0.5 1,913,991 - Pf1N1B4_1670 0.11 -0.5 1,913,991 - Pf1N1B4_1670 0.11 -0.1 1,914,075 + Pf1N1B4_1670 0.24 -0.9 1,914,076 - Pf1N1B4_1670 0.24 +1.5 1,914,117 - Pf1N1B4_1670 0.30 -0.1 1,914,117 - Pf1N1B4_1670 0.30 +0.5 1,914,117 - Pf1N1B4_1670 0.30 -0.1 1,914,117 - Pf1N1B4_1670 0.30 -1.1 1,914,252 + Pf1N1B4_1670 0.50 -0.1 1,914,252 + Pf1N1B4_1670 0.50 -0.7 1,914,253 - Pf1N1B4_1670 0.51 +0.5
Or see this region's nucleotide sequence