Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_165

Experiment: L-Isoleucine (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_163 and Pf1N1B4_164 overlap by 4 nucleotidesPf1N1B4_164 and Pf1N1B4_165 are separated by 124 nucleotidesPf1N1B4_165 and Pf1N1B4_166 are separated by 139 nucleotides Pf1N1B4_163 - ADP-ribosylglycohydrolase, at 204,369 to 205,313 _163 Pf1N1B4_164 - ADP-ribosylglycohydrolase family protein, at 205,310 to 205,708 _164 Pf1N1B4_165 - FIG00638667: hypothetical protein, at 205,833 to 206,210 _165 Pf1N1B4_166 - Quinohemoprotein amine dehydrogenase beta subunit (EC 1.4.99.-), at 206,350 to 207,468 _166 Position (kb) 205 206 207Strain fitness (log2 ratio) -2 -1 0 1 2at 205.176 kb on + strand, within Pf1N1B4_163at 205.315 kb on + strandat 205.353 kb on - strand, within Pf1N1B4_164at 205.497 kb on - strand, within Pf1N1B4_164at 205.497 kb on - strand, within Pf1N1B4_164at 205.581 kb on + strand, within Pf1N1B4_164at 205.582 kb on - strand, within Pf1N1B4_164at 205.582 kb on - strand, within Pf1N1B4_164at 205.670 kb on + strandat 205.670 kb on + strandat 205.706 kb on + strandat 205.706 kb on + strandat 205.706 kb on + strandat 205.706 kb on + strandat 205.707 kb on - strandat 205.707 kb on - strandat 206.094 kb on + strand, within Pf1N1B4_165at 206.107 kb on - strand, within Pf1N1B4_165at 206.160 kb on + strandat 206.160 kb on + strand, within Pf1N1B4_165at 206.160 kb on + strand, within Pf1N1B4_165at 206.160 kb on + strand, within Pf1N1B4_165at 206.160 kb on + strand, within Pf1N1B4_165at 206.160 kb on + strandat 206.160 kb on + strand, within Pf1N1B4_165at 206.161 kb on - strandat 206.295 kb on + strandat 206.296 kb on - strandat 206.471 kb on + strand, within Pf1N1B4_166at 206.507 kb on + strand, within Pf1N1B4_166at 206.510 kb on + strand, within Pf1N1B4_166at 206.510 kb on + strandat 206.510 kb on + strand, within Pf1N1B4_166at 206.510 kb on + strand, within Pf1N1B4_166at 206.510 kb on + strandat 206.511 kb on - strand, within Pf1N1B4_166at 206.511 kb on - strand, within Pf1N1B4_166at 206.561 kb on + strand, within Pf1N1B4_166at 206.562 kb on - strand, within Pf1N1B4_166at 206.630 kb on + strand, within Pf1N1B4_166at 206.630 kb on + strand, within Pf1N1B4_166at 206.631 kb on - strand, within Pf1N1B4_166at 206.759 kb on + strand, within Pf1N1B4_166at 206.783 kb on + strand, within Pf1N1B4_166at 206.784 kb on - strandat 206.804 kb on + strand, within Pf1N1B4_166at 206.804 kb on + strand, within Pf1N1B4_166at 206.804 kb on + strandat 206.805 kb on + strand, within Pf1N1B4_166at 206.805 kb on - strand, within Pf1N1B4_166at 206.805 kb on - strand, within Pf1N1B4_166at 206.805 kb on - strand, within Pf1N1B4_166at 206.826 kb on - strand, within Pf1N1B4_166at 206.826 kb on - strand, within Pf1N1B4_166at 206.895 kb on - strand, within Pf1N1B4_166at 206.915 kb on + strand, within Pf1N1B4_166at 206.915 kb on + strand, within Pf1N1B4_166at 206.915 kb on + strand, within Pf1N1B4_166at 206.916 kb on - strand, within Pf1N1B4_166at 206.916 kb on - strand, within Pf1N1B4_166at 207.012 kb on - strand, within Pf1N1B4_166at 207.041 kb on + strand, within Pf1N1B4_166

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Isoleucine (C)
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205,176 + Pf1N1B4_163 0.85 -1.5
205,315 + +0.3
205,353 - Pf1N1B4_164 0.11 -0.3
205,497 - Pf1N1B4_164 0.47 -2.6
205,497 - Pf1N1B4_164 0.47 +0.4
205,581 + Pf1N1B4_164 0.68 +2.5
205,582 - Pf1N1B4_164 0.68 +0.1
205,582 - Pf1N1B4_164 0.68 +1.3
205,670 + -0.9
205,670 + +0.7
205,706 + +0.1
205,706 + +0.7
205,706 + -0.9
205,706 + +0.7
205,707 - +0.1
205,707 - +0.7
206,094 + Pf1N1B4_165 0.69 -1.3
206,107 - Pf1N1B4_165 0.72 +0.7
206,160 + -0.3
206,160 + Pf1N1B4_165 0.87 -1.3
206,160 + Pf1N1B4_165 0.87 -2.3
206,160 + Pf1N1B4_165 0.87 -0.7
206,160 + Pf1N1B4_165 0.87 -0.3
206,160 + -1.3
206,160 + Pf1N1B4_165 0.87 -0.9
206,161 - -0.3
206,295 + -0.7
206,296 - -0.3
206,471 + Pf1N1B4_166 0.11 +0.7
206,507 + Pf1N1B4_166 0.14 +0.7
206,510 + Pf1N1B4_166 0.14 +0.3
206,510 + -0.3
206,510 + Pf1N1B4_166 0.14 -2.1
206,510 + Pf1N1B4_166 0.14 -0.9
206,510 + +0.7
206,511 - Pf1N1B4_166 0.14 -2.0
206,511 - Pf1N1B4_166 0.14 -1.3
206,561 + Pf1N1B4_166 0.19 +1.0
206,562 - Pf1N1B4_166 0.19 +0.1
206,630 + Pf1N1B4_166 0.25 -2.0
206,630 + Pf1N1B4_166 0.25 -0.7
206,631 - Pf1N1B4_166 0.25 +0.4
206,759 + Pf1N1B4_166 0.37 -0.5
206,783 + Pf1N1B4_166 0.39 -0.0
206,784 - +1.7
206,804 + Pf1N1B4_166 0.41 +1.7
206,804 + Pf1N1B4_166 0.41 +0.7
206,804 + +0.3
206,805 + Pf1N1B4_166 0.41 -2.3
206,805 - Pf1N1B4_166 0.41 -1.0
206,805 - Pf1N1B4_166 0.41 +1.0
206,805 - Pf1N1B4_166 0.41 +2.0
206,826 - Pf1N1B4_166 0.43 -1.7
206,826 - Pf1N1B4_166 0.43 +0.3
206,895 - Pf1N1B4_166 0.49 -0.3
206,915 + Pf1N1B4_166 0.50 -0.3
206,915 + Pf1N1B4_166 0.50 +1.3
206,915 + Pf1N1B4_166 0.50 -1.9
206,916 - Pf1N1B4_166 0.51 -1.3
206,916 - Pf1N1B4_166 0.51 -0.7
207,012 - Pf1N1B4_166 0.59 -1.0
207,041 + Pf1N1B4_166 0.62 -0.9

Or see this region's nucleotide sequence