Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1611 and Pf1N1B4_1612 are separated by 630 nucleotides Pf1N1B4_1612 and Pf1N1B4_1613 overlap by 2 nucleotides Pf1N1B4_1613 and Pf1N1B4_1614 are separated by 205 nucleotides
Pf1N1B4_1611 - Nitrogen regulation protein NR(I), at 1,855,823 to 1,857,259
_1611
Pf1N1B4_1612 - FIG00955429: hypothetical protein, at 1,857,890 to 1,858,330
_1612
Pf1N1B4_1613 - tRNA (cytidine(34)-2'-O)-methyltransferase (EC 2.1.1.207), at 1,858,329 to 1,858,784
_1613
Pf1N1B4_1614 - Protein export cytoplasm chaperone protein (SecB, maintains protein to be exported in unfolded state), at 1,858,990 to 1,859,475
_1614
Position (kb)
1857
1858
1859 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1857.184 kb on + strand at 1857.184 kb on + strand at 1857.185 kb on - strand at 1857.185 kb on - strand at 1857.185 kb on - strand at 1857.185 kb on - strand at 1857.185 kb on - strand at 1857.257 kb on + strand at 1857.257 kb on + strand at 1857.257 kb on + strand at 1857.257 kb on + strand at 1857.257 kb on + strand at 1857.257 kb on + strand at 1857.258 kb on - strand at 1857.262 kb on - strand at 1857.626 kb on + strand at 1857.627 kb on - strand at 1857.627 kb on - strand at 1857.653 kb on + strand at 1857.653 kb on + strand at 1857.653 kb on + strand at 1857.654 kb on - strand at 1857.654 kb on - strand at 1857.654 kb on - strand at 1857.654 kb on - strand at 1857.654 kb on - strand at 1857.708 kb on + strand at 1857.708 kb on + strand at 1857.734 kb on + strand at 1857.734 kb on + strand at 1857.734 kb on + strand at 1857.812 kb on + strand at 1857.812 kb on + strand at 1857.812 kb on + strand at 1857.813 kb on - strand at 1857.813 kb on - strand at 1857.813 kb on - strand at 1857.864 kb on + strand at 1857.865 kb on - strand at 1857.891 kb on + strand at 1857.891 kb on + strand at 1857.891 kb on + strand at 1857.891 kb on + strand at 1857.891 kb on + strand at 1857.891 kb on + strand at 1857.891 kb on + strand at 1857.892 kb on - strand at 1857.892 kb on - strand at 1857.892 kb on - strand at 1858.079 kb on + strand, within Pf1N1B4_1612 at 1858.080 kb on - strand, within Pf1N1B4_1612 at 1858.080 kb on - strand, within Pf1N1B4_1612 at 1858.265 kb on + strand, within Pf1N1B4_1612 at 1858.265 kb on + strand, within Pf1N1B4_1612 at 1858.265 kb on + strand, within Pf1N1B4_1612 at 1858.294 kb on + strand at 1858.312 kb on + strand at 1858.312 kb on + strand at 1858.313 kb on - strand at 1858.313 kb on - strand at 1858.489 kb on - strand, within Pf1N1B4_1613 at 1858.489 kb on - strand, within Pf1N1B4_1613 at 1858.489 kb on - strand, within Pf1N1B4_1613 at 1858.563 kb on - strand, within Pf1N1B4_1613 at 1858.752 kb on + strand at 1858.783 kb on - strand at 1859.025 kb on + strand at 1859.025 kb on + strand at 1859.025 kb on + strand at 1859.025 kb on + strand at 1859.025 kb on + strand at 1859.026 kb on - strand at 1859.026 kb on - strand at 1859.026 kb on - strand at 1859.026 kb on - strand at 1859.026 kb on - strand at 1859.026 kb on - strand at 1859.026 kb on - strand at 1859.026 kb on - strand at 1859.026 kb on - strand at 1859.026 kb on - strand at 1859.026 kb on - strand at 1859.026 kb on - strand at 1859.045 kb on + strand, within Pf1N1B4_1614 at 1859.045 kb on + strand, within Pf1N1B4_1614 at 1859.046 kb on - strand, within Pf1N1B4_1614 at 1859.046 kb on - strand, within Pf1N1B4_1614 at 1859.046 kb on - strand, within Pf1N1B4_1614
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 1,857,184 + -1.1 1,857,184 + -0.9 1,857,185 - -0.6 1,857,185 - -1.6 1,857,185 - -0.8 1,857,185 - +2.0 1,857,185 - -0.6 1,857,257 + -1.1 1,857,257 + -0.4 1,857,257 + +0.4 1,857,257 + -0.4 1,857,257 + +2.0 1,857,257 + -0.2 1,857,258 - +1.4 1,857,262 - -0.9 1,857,626 + -1.3 1,857,627 - +1.0 1,857,627 - +0.1 1,857,653 + -1.6 1,857,653 + -1.0 1,857,653 + -1.1 1,857,654 - -1.1 1,857,654 - +0.8 1,857,654 - +0.4 1,857,654 - +0.8 1,857,654 - +0.9 1,857,708 + -0.1 1,857,708 + +0.4 1,857,734 + -1.5 1,857,734 + +0.4 1,857,734 + -0.6 1,857,812 + +0.4 1,857,812 + -0.6 1,857,812 + +1.0 1,857,813 - -0.0 1,857,813 - -1.1 1,857,813 - -0.6 1,857,864 + +0.4 1,857,865 - -0.1 1,857,891 + +0.7 1,857,891 + -0.1 1,857,891 + -1.1 1,857,891 + -0.1 1,857,891 + -0.6 1,857,891 + +0.7 1,857,891 + +0.0 1,857,892 - -0.1 1,857,892 - +2.0 1,857,892 - -0.8 1,858,079 + Pf1N1B4_1612 0.43 +0.4 1,858,080 - Pf1N1B4_1612 0.43 -0.6 1,858,080 - Pf1N1B4_1612 0.43 +1.2 1,858,265 + Pf1N1B4_1612 0.85 -0.3 1,858,265 + Pf1N1B4_1612 0.85 +0.3 1,858,265 + Pf1N1B4_1612 0.85 +1.0 1,858,294 + +1.0 1,858,312 + -2.6 1,858,312 + -0.6 1,858,313 - -0.2 1,858,313 - -2.4 1,858,489 - Pf1N1B4_1613 0.35 -2.0 1,858,489 - Pf1N1B4_1613 0.35 -0.3 1,858,489 - Pf1N1B4_1613 0.35 -0.9 1,858,563 - Pf1N1B4_1613 0.51 -0.3 1,858,752 + -1.1 1,858,783 - +1.0 1,859,025 + -0.6 1,859,025 + -0.9 1,859,025 + +0.4 1,859,025 + +0.4 1,859,025 + -0.6 1,859,026 - -3.0 1,859,026 - -0.3 1,859,026 - -0.3 1,859,026 - -1.1 1,859,026 - -0.1 1,859,026 - -0.6 1,859,026 - -0.6 1,859,026 - -0.6 1,859,026 - -1.1 1,859,026 - +0.8 1,859,026 - -1.6 1,859,026 - +0.2 1,859,045 + Pf1N1B4_1614 0.11 -1.1 1,859,045 + Pf1N1B4_1614 0.11 -1.6 1,859,046 - Pf1N1B4_1614 0.12 -0.1 1,859,046 - Pf1N1B4_1614 0.12 +0.4 1,859,046 - Pf1N1B4_1614 0.12 -0.9
Or see this region's nucleotide sequence