Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1588 and Pf1N1B4_1589 are separated by 270 nucleotides Pf1N1B4_1589 and Pf1N1B4_1590 are separated by 23 nucleotides Pf1N1B4_1590 and Pf1N1B4_1591 are separated by 107 nucleotides
Pf1N1B4_1588 - hypothetical protein, at 1,830,424 to 1,830,546
_1588
Pf1N1B4_1589 - Conserved hypothetical protein (perhaps related to histidine degradation), at 1,830,817 to 1,831,386
_1589
Pf1N1B4_1590 - Histidine utilization repressor, at 1,831,410 to 1,832,171
_1590
Pf1N1B4_1591 - Formiminoglutamic iminohydrolase (EC 3.5.3.13) (from data) , at 1,832,279 to 1,833,643
_1591
Position (kb)
1831
1832
1833 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1830.485 kb on - strand, within Pf1N1B4_1588 at 1830.485 kb on - strand, within Pf1N1B4_1588 at 1830.490 kb on + strand, within Pf1N1B4_1588 at 1830.490 kb on + strand, within Pf1N1B4_1588 at 1830.491 kb on - strand, within Pf1N1B4_1588 at 1830.491 kb on - strand, within Pf1N1B4_1588 at 1830.491 kb on - strand, within Pf1N1B4_1588 at 1830.491 kb on - strand, within Pf1N1B4_1588 at 1830.499 kb on - strand, within Pf1N1B4_1588 at 1830.499 kb on - strand, within Pf1N1B4_1588 at 1830.504 kb on + strand, within Pf1N1B4_1588 at 1830.505 kb on - strand, within Pf1N1B4_1588 at 1830.508 kb on + strand, within Pf1N1B4_1588 at 1830.508 kb on + strand, within Pf1N1B4_1588 at 1830.509 kb on - strand, within Pf1N1B4_1588 at 1830.516 kb on + strand, within Pf1N1B4_1588 at 1830.517 kb on - strand, within Pf1N1B4_1588 at 1830.584 kb on + strand at 1830.584 kb on + strand at 1830.584 kb on + strand at 1830.585 kb on - strand at 1830.585 kb on - strand at 1830.585 kb on - strand at 1830.585 kb on - strand at 1830.648 kb on + strand at 1830.759 kb on + strand at 1830.759 kb on + strand at 1830.759 kb on + strand at 1830.759 kb on + strand at 1830.759 kb on + strand at 1830.759 kb on + strand at 1830.760 kb on - strand at 1830.865 kb on - strand at 1830.913 kb on + strand, within Pf1N1B4_1589 at 1831.049 kb on + strand at 1831.050 kb on - strand, within Pf1N1B4_1589 at 1831.050 kb on - strand, within Pf1N1B4_1589 at 1831.136 kb on - strand, within Pf1N1B4_1589 at 1831.345 kb on - strand at 1831.397 kb on + strand at 1831.397 kb on + strand at 1831.397 kb on + strand at 1831.397 kb on + strand at 1831.398 kb on - strand at 1831.398 kb on - strand at 1831.401 kb on + strand at 1831.401 kb on + strand at 1831.402 kb on - strand at 1831.402 kb on - strand at 1831.402 kb on - strand at 1831.411 kb on + strand at 1831.411 kb on + strand at 1831.411 kb on + strand at 1831.411 kb on + strand at 1831.411 kb on + strand at 1831.411 kb on + strand at 1831.412 kb on - strand at 1831.412 kb on - strand at 1831.412 kb on - strand at 1831.621 kb on + strand, within Pf1N1B4_1590 at 1831.621 kb on + strand, within Pf1N1B4_1590 at 1831.621 kb on + strand, within Pf1N1B4_1590 at 1831.622 kb on - strand, within Pf1N1B4_1590 at 1831.622 kb on - strand, within Pf1N1B4_1590 at 1831.622 kb on - strand, within Pf1N1B4_1590 at 1831.622 kb on - strand, within Pf1N1B4_1590 at 1831.627 kb on + strand, within Pf1N1B4_1590 at 1831.627 kb on + strand, within Pf1N1B4_1590 at 1831.627 kb on + strand, within Pf1N1B4_1590 at 1831.627 kb on + strand, within Pf1N1B4_1590 at 1831.628 kb on - strand, within Pf1N1B4_1590 at 1831.628 kb on - strand, within Pf1N1B4_1590 at 1831.660 kb on + strand, within Pf1N1B4_1590 at 1831.660 kb on + strand, within Pf1N1B4_1590 at 1831.660 kb on + strand, within Pf1N1B4_1590 at 1831.661 kb on - strand, within Pf1N1B4_1590 at 1831.661 kb on - strand, within Pf1N1B4_1590 at 1831.713 kb on - strand, within Pf1N1B4_1590 at 1831.916 kb on + strand, within Pf1N1B4_1590 at 1831.916 kb on + strand, within Pf1N1B4_1590 at 1831.916 kb on + strand, within Pf1N1B4_1590 at 1831.917 kb on - strand, within Pf1N1B4_1590 at 1832.095 kb on + strand at 1832.095 kb on + strand at 1832.095 kb on + strand at 1832.095 kb on + strand at 1832.095 kb on + strand at 1832.096 kb on - strand at 1832.096 kb on - strand at 1832.096 kb on - strand at 1832.096 kb on - strand at 1832.203 kb on + strand at 1832.203 kb on + strand at 1832.203 kb on + strand at 1832.203 kb on + strand at 1832.203 kb on + strand at 1832.204 kb on - strand at 1832.239 kb on + strand at 1832.239 kb on + strand at 1832.239 kb on + strand at 1832.239 kb on + strand at 1832.240 kb on - strand at 1832.240 kb on - strand at 1832.240 kb on - strand at 1832.240 kb on - strand at 1832.240 kb on - strand at 1832.240 kb on - strand at 1832.241 kb on + strand at 1832.241 kb on + strand at 1832.242 kb on - strand at 1832.242 kb on - strand at 1832.245 kb on + strand at 1832.245 kb on + strand at 1832.245 kb on + strand at 1832.245 kb on + strand at 1832.245 kb on + strand at 1832.246 kb on - strand at 1832.246 kb on - strand at 1832.246 kb on - strand at 1832.246 kb on - strand at 1832.246 kb on - strand at 1832.246 kb on - strand at 1832.246 kb on - strand at 1832.248 kb on - strand at 1832.248 kb on - strand at 1832.314 kb on + strand at 1832.334 kb on + strand at 1832.334 kb on + strand at 1832.334 kb on + strand at 1832.335 kb on - strand at 1832.335 kb on - strand at 1832.335 kb on - strand at 1832.335 kb on - strand at 1832.335 kb on - strand at 1832.591 kb on + strand, within Pf1N1B4_1591 at 1832.592 kb on - strand, within Pf1N1B4_1591 at 1832.592 kb on - strand, within Pf1N1B4_1591 at 1832.592 kb on - strand, within Pf1N1B4_1591 at 1832.592 kb on - strand, within Pf1N1B4_1591 at 1832.592 kb on - strand, within Pf1N1B4_1591 at 1832.639 kb on + strand, within Pf1N1B4_1591 at 1832.639 kb on + strand, within Pf1N1B4_1591 at 1832.639 kb on + strand, within Pf1N1B4_1591 at 1832.639 kb on + strand, within Pf1N1B4_1591 at 1832.640 kb on - strand, within Pf1N1B4_1591 at 1832.640 kb on - strand, within Pf1N1B4_1591 at 1832.640 kb on - strand, within Pf1N1B4_1591 at 1832.640 kb on - strand, within Pf1N1B4_1591 at 1832.643 kb on + strand, within Pf1N1B4_1591 at 1832.643 kb on + strand, within Pf1N1B4_1591 at 1832.643 kb on + strand, within Pf1N1B4_1591 at 1832.643 kb on + strand, within Pf1N1B4_1591 at 1832.643 kb on + strand, within Pf1N1B4_1591 at 1832.643 kb on + strand, within Pf1N1B4_1591 at 1832.644 kb on - strand, within Pf1N1B4_1591 at 1832.644 kb on - strand, within Pf1N1B4_1591 at 1832.644 kb on - strand, within Pf1N1B4_1591 at 1832.753 kb on + strand, within Pf1N1B4_1591 at 1832.753 kb on + strand, within Pf1N1B4_1591 at 1832.753 kb on + strand, within Pf1N1B4_1591 at 1832.754 kb on - strand, within Pf1N1B4_1591 at 1832.754 kb on - strand, within Pf1N1B4_1591 at 1832.754 kb on - strand, within Pf1N1B4_1591 at 1832.807 kb on - strand, within Pf1N1B4_1591 at 1832.825 kb on + strand, within Pf1N1B4_1591 at 1832.826 kb on - strand, within Pf1N1B4_1591 at 1832.826 kb on - strand, within Pf1N1B4_1591 at 1833.047 kb on + strand, within Pf1N1B4_1591 at 1833.048 kb on - strand, within Pf1N1B4_1591 at 1833.150 kb on + strand, within Pf1N1B4_1591 at 1833.150 kb on + strand, within Pf1N1B4_1591 at 1833.150 kb on + strand, within Pf1N1B4_1591 at 1833.150 kb on + strand, within Pf1N1B4_1591 at 1833.151 kb on - strand, within Pf1N1B4_1591 at 1833.151 kb on - strand, within Pf1N1B4_1591 at 1833.151 kb on - strand, within Pf1N1B4_1591 at 1833.151 kb on - strand, within Pf1N1B4_1591
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 1,830,485 - Pf1N1B4_1588 0.50 -0.0 1,830,485 - Pf1N1B4_1588 0.50 -0.1 1,830,490 + Pf1N1B4_1588 0.54 -0.6 1,830,490 + Pf1N1B4_1588 0.54 -1.0 1,830,491 - Pf1N1B4_1588 0.54 +0.4 1,830,491 - Pf1N1B4_1588 0.54 -2.7 1,830,491 - Pf1N1B4_1588 0.54 -2.0 1,830,491 - Pf1N1B4_1588 0.54 +0.1 1,830,499 - Pf1N1B4_1588 0.61 -0.4 1,830,499 - Pf1N1B4_1588 0.61 +0.3 1,830,504 + Pf1N1B4_1588 0.65 -0.5 1,830,505 - Pf1N1B4_1588 0.66 -1.3 1,830,508 + Pf1N1B4_1588 0.68 +0.0 1,830,508 + Pf1N1B4_1588 0.68 -0.8 1,830,509 - Pf1N1B4_1588 0.69 -1.5 1,830,516 + Pf1N1B4_1588 0.75 -1.9 1,830,517 - Pf1N1B4_1588 0.76 +0.4 1,830,584 + +0.6 1,830,584 + -1.1 1,830,584 + +1.4 1,830,585 - -0.0 1,830,585 - -0.1 1,830,585 - -1.6 1,830,585 - +1.4 1,830,648 + +0.4 1,830,759 + +0.4 1,830,759 + +1.4 1,830,759 + -0.7 1,830,759 + +1.2 1,830,759 + -1.1 1,830,759 + +0.4 1,830,760 - +1.1 1,830,865 - -1.6 1,830,913 + Pf1N1B4_1589 0.17 +1.4 1,831,049 + -0.1 1,831,050 - Pf1N1B4_1589 0.41 +1.0 1,831,050 - Pf1N1B4_1589 0.41 +0.1 1,831,136 - Pf1N1B4_1589 0.56 -0.3 1,831,345 - +2.2 1,831,397 + -0.1 1,831,397 + -1.1 1,831,397 + +0.4 1,831,397 + -1.9 1,831,398 - +0.9 1,831,398 - -0.6 1,831,401 + +0.1 1,831,401 + -2.1 1,831,402 - -0.1 1,831,402 - -0.2 1,831,402 - +0.2 1,831,411 + -0.6 1,831,411 + -1.1 1,831,411 + -0.6 1,831,411 + +2.0 1,831,411 + -1.6 1,831,411 + -1.1 1,831,412 - +1.0 1,831,412 - +0.4 1,831,412 - +0.4 1,831,621 + Pf1N1B4_1590 0.28 -0.6 1,831,621 + Pf1N1B4_1590 0.28 -1.9 1,831,621 + Pf1N1B4_1590 0.28 -0.4 1,831,622 - Pf1N1B4_1590 0.28 +0.4 1,831,622 - Pf1N1B4_1590 0.28 -0.6 1,831,622 - Pf1N1B4_1590 0.28 +0.4 1,831,622 - Pf1N1B4_1590 0.28 -1.1 1,831,627 + Pf1N1B4_1590 0.28 -1.1 1,831,627 + Pf1N1B4_1590 0.28 -1.3 1,831,627 + Pf1N1B4_1590 0.28 +0.8 1,831,627 + Pf1N1B4_1590 0.28 -1.4 1,831,628 - Pf1N1B4_1590 0.29 -1.1 1,831,628 - Pf1N1B4_1590 0.29 -1.5 1,831,660 + Pf1N1B4_1590 0.33 -1.1 1,831,660 + Pf1N1B4_1590 0.33 -1.1 1,831,660 + Pf1N1B4_1590 0.33 -2.1 1,831,661 - Pf1N1B4_1590 0.33 -2.1 1,831,661 - Pf1N1B4_1590 0.33 -1.6 1,831,713 - Pf1N1B4_1590 0.40 -1.1 1,831,916 + Pf1N1B4_1590 0.66 -1.9 1,831,916 + Pf1N1B4_1590 0.66 -0.1 1,831,916 + Pf1N1B4_1590 0.66 +2.0 1,831,917 - Pf1N1B4_1590 0.67 -2.4 1,832,095 + -1.1 1,832,095 + -1.9 1,832,095 + +0.4 1,832,095 + +0.1 1,832,095 + -1.1 1,832,096 - -1.0 1,832,096 - -0.8 1,832,096 - -1.1 1,832,096 - -0.1 1,832,203 + -1.6 1,832,203 + -1.9 1,832,203 + +2.0 1,832,203 + -1.0 1,832,203 + -0.9 1,832,204 - +0.4 1,832,239 + -0.0 1,832,239 + +0.4 1,832,239 + -0.7 1,832,239 + -0.8 1,832,240 - +0.2 1,832,240 - -0.8 1,832,240 - +1.4 1,832,240 - -0.3 1,832,240 - +0.4 1,832,240 - -0.4 1,832,241 + -0.1 1,832,241 + -2.1 1,832,242 - +1.8 1,832,242 - +0.4 1,832,245 + -0.5 1,832,245 + -2.1 1,832,245 + -0.1 1,832,245 + -0.4 1,832,245 + -0.6 1,832,246 - +0.4 1,832,246 - -3.1 1,832,246 - -0.1 1,832,246 - -0.6 1,832,246 - +0.9 1,832,246 - -1.1 1,832,246 - -0.0 1,832,248 - -0.7 1,832,248 - +1.4 1,832,314 + -0.3 1,832,334 + +1.4 1,832,334 + -2.1 1,832,334 + -1.1 1,832,335 - -1.1 1,832,335 - -0.4 1,832,335 - -2.1 1,832,335 - -0.8 1,832,335 - -0.6 1,832,591 + Pf1N1B4_1591 0.23 -0.1 1,832,592 - Pf1N1B4_1591 0.23 +0.2 1,832,592 - Pf1N1B4_1591 0.23 +0.4 1,832,592 - Pf1N1B4_1591 0.23 -0.6 1,832,592 - Pf1N1B4_1591 0.23 +0.9 1,832,592 - Pf1N1B4_1591 0.23 -1.6 1,832,639 + Pf1N1B4_1591 0.26 +1.2 1,832,639 + Pf1N1B4_1591 0.26 -1.6 1,832,639 + Pf1N1B4_1591 0.26 -1.6 1,832,639 + Pf1N1B4_1591 0.26 -0.1 1,832,640 - Pf1N1B4_1591 0.26 -0.6 1,832,640 - Pf1N1B4_1591 0.26 +0.6 1,832,640 - Pf1N1B4_1591 0.26 -0.3 1,832,640 - Pf1N1B4_1591 0.26 +0.4 1,832,643 + Pf1N1B4_1591 0.27 +0.1 1,832,643 + Pf1N1B4_1591 0.27 +0.6 1,832,643 + Pf1N1B4_1591 0.27 +1.4 1,832,643 + Pf1N1B4_1591 0.27 -1.0 1,832,643 + Pf1N1B4_1591 0.27 +1.8 1,832,643 + Pf1N1B4_1591 0.27 -0.1 1,832,644 - Pf1N1B4_1591 0.27 +1.4 1,832,644 - Pf1N1B4_1591 0.27 +1.0 1,832,644 - Pf1N1B4_1591 0.27 +0.4 1,832,753 + Pf1N1B4_1591 0.35 -0.4 1,832,753 + Pf1N1B4_1591 0.35 -1.9 1,832,753 + Pf1N1B4_1591 0.35 +0.8 1,832,754 - Pf1N1B4_1591 0.35 +0.4 1,832,754 - Pf1N1B4_1591 0.35 +0.4 1,832,754 - Pf1N1B4_1591 0.35 +0.4 1,832,807 - Pf1N1B4_1591 0.39 -0.8 1,832,825 + Pf1N1B4_1591 0.40 +0.1 1,832,826 - Pf1N1B4_1591 0.40 -0.3 1,832,826 - Pf1N1B4_1591 0.40 -1.7 1,833,047 + Pf1N1B4_1591 0.56 +1.0 1,833,048 - Pf1N1B4_1591 0.56 +0.5 1,833,150 + Pf1N1B4_1591 0.64 -0.1 1,833,150 + Pf1N1B4_1591 0.64 +0.8 1,833,150 + Pf1N1B4_1591 0.64 -0.7 1,833,150 + Pf1N1B4_1591 0.64 +2.0 1,833,151 - Pf1N1B4_1591 0.64 +0.0 1,833,151 - Pf1N1B4_1591 0.64 +1.4 1,833,151 - Pf1N1B4_1591 0.64 -1.6 1,833,151 - Pf1N1B4_1591 0.64 -2.1
Or see this region's nucleotide sequence