Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1564 and Pf1N1B4_1565 are separated by 90 nucleotides Pf1N1B4_1565 and Pf1N1B4_1566 overlap by 8 nucleotides Pf1N1B4_1566 and Pf1N1B4_1567 are separated by 24 nucleotides Pf1N1B4_1567 and Pf1N1B4_1568 are separated by 10 nucleotides
Pf1N1B4_1564 - Ubiquinone biosynthesis monooxygenase UbiB, at 1,801,976 to 1,803,580
_1564
Pf1N1B4_1565 - Phosphoribosyl-AMP cyclohydrolase (EC 3.5.4.19), at 1,803,671 to 1,804,072
_1565
Pf1N1B4_1566 - phosphoribosyl-ATP pyrophosphatase (EC 3.6.1.31) (from data) , at 1,804,065 to 1,804,397
_1566
Pf1N1B4_1567 - Twin-arginine translocation protein TatA, at 1,804,422 to 1,804,700
_1567
Pf1N1B4_1568 - Twin-arginine translocation protein TatB, at 1,804,711 to 1,805,136
_1568
Position (kb)
1803
1804
1805 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1803.640 kb on + strand at 1803.640 kb on + strand at 1803.640 kb on + strand at 1803.640 kb on + strand at 1803.641 kb on - strand at 1803.641 kb on - strand at 1803.641 kb on - strand at 1803.641 kb on - strand at 1803.641 kb on - strand at 1803.641 kb on - strand at 1803.641 kb on - strand at 1803.641 kb on - strand at 1803.641 kb on - strand at 1803.641 kb on - strand at 1803.641 kb on - strand at 1803.641 kb on - strand at 1803.641 kb on - strand at 1803.648 kb on + strand at 1803.648 kb on + strand at 1803.648 kb on + strand at 1803.648 kb on + strand at 1803.648 kb on + strand at 1803.649 kb on - strand at 1803.649 kb on - strand at 1803.649 kb on - strand at 1803.649 kb on - strand at 1803.649 kb on - strand at 1803.649 kb on - strand at 1803.649 kb on - strand at 1803.649 kb on - strand at 1803.818 kb on + strand, within Pf1N1B4_1565 at 1803.818 kb on + strand, within Pf1N1B4_1565 at 1803.818 kb on + strand, within Pf1N1B4_1565 at 1803.818 kb on + strand, within Pf1N1B4_1565 at 1803.819 kb on - strand, within Pf1N1B4_1565 at 1803.819 kb on - strand, within Pf1N1B4_1565 at 1803.819 kb on - strand, within Pf1N1B4_1565 at 1803.819 kb on - strand, within Pf1N1B4_1565 at 1803.819 kb on - strand, within Pf1N1B4_1565 at 1803.819 kb on - strand, within Pf1N1B4_1565 at 1803.980 kb on + strand, within Pf1N1B4_1565 at 1803.981 kb on - strand, within Pf1N1B4_1565 at 1803.981 kb on - strand, within Pf1N1B4_1565 at 1804.028 kb on - strand at 1804.028 kb on - strand, within Pf1N1B4_1565 at 1804.043 kb on - strand at 1804.043 kb on - strand at 1804.043 kb on - strand at 1804.043 kb on - strand at 1804.043 kb on - strand at 1804.046 kb on + strand at 1804.046 kb on + strand at 1804.046 kb on + strand at 1804.047 kb on - strand at 1804.047 kb on - strand at 1804.047 kb on - strand at 1804.047 kb on - strand at 1804.047 kb on - strand at 1804.047 kb on - strand at 1804.060 kb on + strand at 1804.060 kb on + strand at 1804.060 kb on + strand at 1804.060 kb on + strand at 1804.061 kb on - strand at 1804.061 kb on - strand at 1804.061 kb on - strand at 1804.061 kb on - strand at 1804.061 kb on - strand at 1804.061 kb on - strand at 1804.061 kb on - strand at 1804.061 kb on - strand at 1804.061 kb on - strand at 1804.061 kb on - strand at 1804.061 kb on - strand at 1804.061 kb on - strand at 1804.061 kb on - strand at 1804.061 kb on - strand at 1804.061 kb on - strand at 1804.061 kb on - strand at 1804.061 kb on - strand at 1804.061 kb on - strand at 1804.133 kb on + strand, within Pf1N1B4_1566 at 1804.134 kb on + strand, within Pf1N1B4_1566 at 1804.134 kb on + strand, within Pf1N1B4_1566 at 1804.134 kb on + strand at 1804.134 kb on + strand, within Pf1N1B4_1566 at 1804.134 kb on + strand, within Pf1N1B4_1566 at 1804.134 kb on + strand, within Pf1N1B4_1566 at 1804.134 kb on + strand, within Pf1N1B4_1566 at 1804.135 kb on - strand, within Pf1N1B4_1566 at 1804.135 kb on - strand, within Pf1N1B4_1566 at 1804.135 kb on - strand, within Pf1N1B4_1566 at 1804.135 kb on - strand, within Pf1N1B4_1566 at 1804.135 kb on - strand, within Pf1N1B4_1566 at 1804.135 kb on - strand, within Pf1N1B4_1566 at 1804.135 kb on - strand, within Pf1N1B4_1566 at 1804.135 kb on - strand, within Pf1N1B4_1566 at 1804.135 kb on - strand, within Pf1N1B4_1566 at 1804.135 kb on - strand, within Pf1N1B4_1566 at 1804.135 kb on - strand, within Pf1N1B4_1566 at 1804.135 kb on - strand, within Pf1N1B4_1566 at 1804.135 kb on - strand, within Pf1N1B4_1566 at 1804.135 kb on - strand, within Pf1N1B4_1566 at 1804.135 kb on - strand, within Pf1N1B4_1566 at 1804.135 kb on - strand, within Pf1N1B4_1566 at 1804.135 kb on - strand, within Pf1N1B4_1566 at 1804.135 kb on - strand, within Pf1N1B4_1566 at 1804.135 kb on - strand, within Pf1N1B4_1566 at 1804.135 kb on - strand, within Pf1N1B4_1566 at 1804.135 kb on - strand, within Pf1N1B4_1566 at 1804.135 kb on - strand, within Pf1N1B4_1566 at 1804.135 kb on - strand, within Pf1N1B4_1566 at 1804.135 kb on - strand, within Pf1N1B4_1566 at 1804.149 kb on + strand, within Pf1N1B4_1566 at 1804.150 kb on - strand, within Pf1N1B4_1566 at 1804.150 kb on - strand, within Pf1N1B4_1566 at 1804.258 kb on - strand, within Pf1N1B4_1566 at 1804.258 kb on - strand, within Pf1N1B4_1566 at 1804.258 kb on - strand, within Pf1N1B4_1566 at 1804.403 kb on + strand at 1804.403 kb on + strand at 1804.403 kb on + strand at 1804.403 kb on + strand at 1804.404 kb on - strand at 1804.404 kb on - strand at 1804.404 kb on - strand at 1804.404 kb on - strand at 1804.404 kb on - strand at 1804.404 kb on - strand at 1804.404 kb on - strand at 1804.404 kb on - strand at 1804.404 kb on - strand at 1804.404 kb on - strand at 1804.404 kb on - strand at 1804.404 kb on - strand at 1804.404 kb on - strand at 1804.404 kb on - strand at 1804.404 kb on - strand at 1804.404 kb on - strand at 1804.404 kb on - strand at 1804.404 kb on - strand at 1804.404 kb on - strand at 1804.541 kb on + strand, within Pf1N1B4_1567 at 1804.541 kb on + strand, within Pf1N1B4_1567 at 1804.541 kb on + strand, within Pf1N1B4_1567 at 1804.542 kb on - strand, within Pf1N1B4_1567 at 1804.709 kb on + strand at 1804.709 kb on + strand at 1804.709 kb on + strand at 1804.709 kb on + strand at 1804.709 kb on + strand at 1804.709 kb on + strand at 1804.709 kb on + strand at 1804.709 kb on + strand at 1804.709 kb on + strand at 1804.709 kb on + strand at 1804.710 kb on - strand at 1804.710 kb on - strand at 1804.710 kb on - strand at 1804.710 kb on - strand at 1804.710 kb on - strand at 1804.710 kb on - strand at 1804.710 kb on - strand at 1804.710 kb on - strand at 1804.710 kb on - strand at 1804.710 kb on - strand at 1804.719 kb on + strand at 1804.719 kb on + strand at 1804.719 kb on + strand at 1804.720 kb on - strand at 1804.720 kb on - strand at 1804.986 kb on - strand, within Pf1N1B4_1568 at 1805.060 kb on + strand, within Pf1N1B4_1568 at 1805.061 kb on - strand, within Pf1N1B4_1568 at 1805.061 kb on - strand, within Pf1N1B4_1568 at 1805.061 kb on - strand, within Pf1N1B4_1568 at 1805.061 kb on - strand, within Pf1N1B4_1568 at 1805.061 kb on - strand, within Pf1N1B4_1568 at 1805.061 kb on - strand, within Pf1N1B4_1568 at 1805.061 kb on - strand, within Pf1N1B4_1568
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 1,803,640 + -1.7 1,803,640 + +0.4 1,803,640 + -1.2 1,803,640 + +0.4 1,803,641 - -1.9 1,803,641 - +1.4 1,803,641 - -3.6 1,803,641 - -0.5 1,803,641 - -1.2 1,803,641 - -0.6 1,803,641 - -1.9 1,803,641 - -2.2 1,803,641 - -2.2 1,803,641 - -0.9 1,803,641 - -0.7 1,803,641 - -2.7 1,803,641 - +0.8 1,803,648 + -0.3 1,803,648 + +2.0 1,803,648 + +0.4 1,803,648 + +0.4 1,803,648 + -0.0 1,803,649 - -0.6 1,803,649 - -2.8 1,803,649 - -2.2 1,803,649 - -2.2 1,803,649 - -1.6 1,803,649 - -2.7 1,803,649 - -3.2 1,803,649 - +0.4 1,803,818 + Pf1N1B4_1565 0.37 -1.6 1,803,818 + Pf1N1B4_1565 0.37 -2.7 1,803,818 + Pf1N1B4_1565 0.37 -0.6 1,803,818 + Pf1N1B4_1565 0.37 -1.2 1,803,819 - Pf1N1B4_1565 0.37 -1.9 1,803,819 - Pf1N1B4_1565 0.37 -1.6 1,803,819 - Pf1N1B4_1565 0.37 -2.2 1,803,819 - Pf1N1B4_1565 0.37 -2.6 1,803,819 - Pf1N1B4_1565 0.37 -1.6 1,803,819 - Pf1N1B4_1565 0.37 -0.6 1,803,980 + Pf1N1B4_1565 0.77 -1.6 1,803,981 - Pf1N1B4_1565 0.77 -1.6 1,803,981 - Pf1N1B4_1565 0.77 -1.9 1,804,028 - +0.4 1,804,028 - Pf1N1B4_1565 0.89 -1.6 1,804,043 - -1.9 1,804,043 - -3.0 1,804,043 - +0.4 1,804,043 - -3.3 1,804,043 - -1.6 1,804,046 + +2.2 1,804,046 + +0.4 1,804,046 + -0.4 1,804,047 - -1.2 1,804,047 - -0.7 1,804,047 - -0.6 1,804,047 - -0.6 1,804,047 - -3.0 1,804,047 - -0.9 1,804,060 + -2.4 1,804,060 + -1.6 1,804,060 + -3.0 1,804,060 + -1.6 1,804,061 - -4.3 1,804,061 - -2.2 1,804,061 - -1.6 1,804,061 - -2.7 1,804,061 - -1.9 1,804,061 - -2.7 1,804,061 - -2.4 1,804,061 - -1.4 1,804,061 - -0.3 1,804,061 - -0.9 1,804,061 - -0.2 1,804,061 - -3.2 1,804,061 - -1.7 1,804,061 - -2.4 1,804,061 - -1.1 1,804,061 - -3.0 1,804,061 - -1.9 1,804,061 - -1.9 1,804,133 + Pf1N1B4_1566 0.20 -0.6 1,804,134 + Pf1N1B4_1566 0.21 -2.6 1,804,134 + Pf1N1B4_1566 0.21 -3.4 1,804,134 + +0.4 1,804,134 + Pf1N1B4_1566 0.21 -1.2 1,804,134 + Pf1N1B4_1566 0.21 +0.0 1,804,134 + Pf1N1B4_1566 0.21 -1.2 1,804,134 + Pf1N1B4_1566 0.21 -1.9 1,804,135 - Pf1N1B4_1566 0.21 +0.7 1,804,135 - Pf1N1B4_1566 0.21 +1.2 1,804,135 - Pf1N1B4_1566 0.21 -1.8 1,804,135 - Pf1N1B4_1566 0.21 -1.3 1,804,135 - Pf1N1B4_1566 0.21 -0.3 1,804,135 - Pf1N1B4_1566 0.21 -0.4 1,804,135 - Pf1N1B4_1566 0.21 -2.2 1,804,135 - Pf1N1B4_1566 0.21 +0.4 1,804,135 - Pf1N1B4_1566 0.21 -2.7 1,804,135 - Pf1N1B4_1566 0.21 -1.6 1,804,135 - Pf1N1B4_1566 0.21 -2.7 1,804,135 - Pf1N1B4_1566 0.21 +0.4 1,804,135 - Pf1N1B4_1566 0.21 +0.4 1,804,135 - Pf1N1B4_1566 0.21 -2.9 1,804,135 - Pf1N1B4_1566 0.21 -1.6 1,804,135 - Pf1N1B4_1566 0.21 -0.6 1,804,135 - Pf1N1B4_1566 0.21 -0.9 1,804,135 - Pf1N1B4_1566 0.21 -1.7 1,804,135 - Pf1N1B4_1566 0.21 -2.9 1,804,135 - Pf1N1B4_1566 0.21 -2.2 1,804,135 - Pf1N1B4_1566 0.21 -1.6 1,804,135 - Pf1N1B4_1566 0.21 -1.3 1,804,135 - Pf1N1B4_1566 0.21 -0.9 1,804,135 - Pf1N1B4_1566 0.21 -3.7 1,804,149 + Pf1N1B4_1566 0.25 -1.6 1,804,150 - Pf1N1B4_1566 0.26 -0.6 1,804,150 - Pf1N1B4_1566 0.26 -0.2 1,804,258 - Pf1N1B4_1566 0.58 +0.4 1,804,258 - Pf1N1B4_1566 0.58 -3.0 1,804,258 - Pf1N1B4_1566 0.58 -2.6 1,804,403 + -0.9 1,804,403 + -1.2 1,804,403 + +1.0 1,804,403 + +0.2 1,804,404 - +0.4 1,804,404 - +1.0 1,804,404 - +0.0 1,804,404 - -0.6 1,804,404 - -2.7 1,804,404 - -0.4 1,804,404 - -2.4 1,804,404 - -1.2 1,804,404 - -0.3 1,804,404 - -0.9 1,804,404 - +0.4 1,804,404 - -0.4 1,804,404 - -2.2 1,804,404 - +1.4 1,804,404 - +0.1 1,804,404 - +0.8 1,804,404 - -1.6 1,804,404 - -0.6 1,804,404 - -0.6 1,804,541 + Pf1N1B4_1567 0.43 -1.4 1,804,541 + Pf1N1B4_1567 0.43 -1.9 1,804,541 + Pf1N1B4_1567 0.43 +0.4 1,804,542 - Pf1N1B4_1567 0.43 -1.2 1,804,709 + +0.0 1,804,709 + -0.8 1,804,709 + -0.7 1,804,709 + -1.4 1,804,709 + -1.3 1,804,709 + -0.2 1,804,709 + -0.5 1,804,709 + +0.3 1,804,709 + +0.8 1,804,709 + +1.8 1,804,710 - -0.6 1,804,710 - -0.9 1,804,710 - -1.7 1,804,710 - -0.8 1,804,710 - +1.0 1,804,710 - +0.4 1,804,710 - -2.2 1,804,710 - -2.2 1,804,710 - -0.3 1,804,710 - +0.9 1,804,719 + +0.3 1,804,719 + -0.2 1,804,719 + +0.2 1,804,720 - +1.4 1,804,720 - -0.6 1,804,986 - Pf1N1B4_1568 0.65 +0.1 1,805,060 + Pf1N1B4_1568 0.82 -0.3 1,805,061 - Pf1N1B4_1568 0.82 -0.9 1,805,061 - Pf1N1B4_1568 0.82 +1.2 1,805,061 - Pf1N1B4_1568 0.82 -1.4 1,805,061 - Pf1N1B4_1568 0.82 -1.7 1,805,061 - Pf1N1B4_1568 0.82 -1.2 1,805,061 - Pf1N1B4_1568 0.82 -0.6 1,805,061 - Pf1N1B4_1568 0.82 -0.2
Or see this region's nucleotide sequence